MOLECULAR WEIGHT AND DISSOCIATION BEHAVIOR OF α- AND β-LIPOVITELLIN IN ACID SOLVENTS

1962 ◽  
Vol 40 (1) ◽  
pp. 877-883 ◽  
Author(s):  
J. P. Kratohvil ◽  
W. G. Martin ◽  
W. H. Cook
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Hydrochloric Acid ◽  
Formic Acid ◽  
Quantitative Study ◽  
Acid Solutions ◽  
Solubility Behavior ◽  
And Diffusion

Solutions of α-lipovitellin in 1 M formic acid and 0.01 M hydrochloric acid had two sedimenting components of ca. 2.0 S and 4.6 S. Initially, acid solutions of β-lipovitellin contained only the 4.6 S component but, after about 4 days, the 2.0 S component was also present. Using freshly prepared solutions the molecular weight (M) of this 4.6 S component of β-lipovitellin was established as 3.9 × 105 by both light scattering and sedimentation and diffusion measurements. This component appears, therefore, to be the associated form of β-lipovitellin of 11 S and M = 4.0 × 105 in neutral solvents. The 2.0 S component must therefore be a dissociated subunit of comparable or smaller size than the subunit of 7 S and M = 2.0 × 105 in alkaline solvents. Evidently both lipovitellins dissociate in acid, as they do in alkaline solvents, but the solubility behavior and irreversible changes that occur in acid precluded a quantitative study.

MOLECULAR WEIGHT AND DISSOCIATION BEHAVIOR OF α- AND β-LIPOVITELLIN IN ACID SOLVENTS

1962 ◽  
Vol 40 (7) ◽  
pp. 877-883 ◽  
Author(s):  
J. P. Kratohvil ◽  
W. G. Martin ◽  
W. H. Cook
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Hydrochloric Acid ◽  
Formic Acid ◽  
Quantitative Study ◽  
Acid Solutions ◽  
Solubility Behavior ◽  
And Diffusion

Solutions of α-lipovitellin in 1 M formic acid and 0.01 M hydrochloric acid had two sedimenting components of ca. 2.0 S and 4.6 S. Initially, acid solutions of β-lipovitellin contained only the 4.6 S component but, after about 4 days, the 2.0 S component was also present. Using freshly prepared solutions the molecular weight (M) of this 4.6 S component of β-lipovitellin was established as 3.9 × 105 by both light scattering and sedimentation and diffusion measurements. This component appears, therefore, to be the associated form of β-lipovitellin of 11 S and M = 4.0 × 105 in neutral solvents. The 2.0 S component must therefore be a dissociated subunit of comparable or smaller size than the subunit of 7 S and M = 2.0 × 105 in alkaline solvents. Evidently both lipovitellins dissociate in acid, as they do in alkaline solvents, but the solubility behavior and irreversible changes that occur in acid precluded a quantitative study.

scholarly journals COMPLEXATION OF POLYETHYLENE-GLYCOL WITH THE SODIUM SALTS OF CITRIC AND SUCCINIC ACIDS IN THE AQUEOUS SOLUTIONS. STUDIES BY DYNAMIC LIGHT SCATTERING AND UV/VIS SPECTROPHOTOMETRY

2015 ◽  
Vol 11 (8) ◽  
pp. 3866-3872
Author(s):  
E.A. Masimov ◽  
Etibar Hummat Ismailov ◽  
S.Y. Odzhaqverdiyeva
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Aqueous Solutions ◽  
Polyethylene Glycols ◽  
Hydrodynamic Diameter ◽  
Absorption Bands ◽  
Sodium Salts ◽  
And Diffusion ◽  
Formation Of Complexes

Dynamic light scattering (DLS) method in combination with the UV/VIS spectrophotometry is used to study the interaction of polyethylene- glycols with a molecular weight  6000 ( PEG6000 ) with sodium salts of citric and succinic acids in aqueous solutions. The values of density, viscosity, refractive and diffusion indexes, the values of the hydrodynamic diameter, wavelength electronic absorption bands for PEG6000 aqueous solutions, their mixtures with succinic and citric acids are determined. It was shown that depending on the composition of the solutions the values of hydrodynamic diameter for aqueous solutions containing 1-5 wt.% PEG6000 and their mixtures with succinic and citric acids (~ 1 wt%) ranges from 3.6 to 5.2 nm. It is assumed that the formation of complexes with the sizes  that are within the above range is due to the features of interaction  and the structure of the complexes formed in solution.

The Applications of Light Scattering

1971 ◽  
Vol 25 (5) ◽  
pp. 509-516 ◽  
Author(s):  
C. C. Gravatt
Keyword(s):  
Molecular Weight ◽  
Air Pollution ◽  
Light Scattering ◽  
Scattered Radiation ◽  
Time Dependent ◽  
Molecular Weight Determination ◽  
Scattered Intensity ◽  
Static Properties ◽  
Diffusion Phenomena ◽  
And Diffusion

Light scattering provides information concerning the size, shape, number, and time dependence of the physical nonuniformities of a system. Measurements of the scattered intensity as a function of both scattering angle and wavelength describe static properties, whereas the spectral distribution of the scattered radiation is related to time dependent phenomena. The applications of light scattering are discussed, including critical phenomena, molecular weight determination, air pollution analysis, and diffusion phenomena.

scholarly journals Polymeric structure of a high-molecular-weight glycoprotein from bovine cervical mucus

1983 ◽  
Vol 215 (3) ◽  
pp. 701-704 ◽  
Author(s):  
F A Meyer
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
High Molecular Weight ◽  
Bond Cleavage ◽  
Cervical Mucus ◽  
Disulphide Bond ◽  
Polymeric Structure ◽  
Diffusion Data ◽  
And Diffusion

A 16 X 10(6)-Mr glycoprotein isolated from bovine oestrus cervical mucus when reduced under conditions where disulphide-bond cleavage is essentially quantitative produces chains whose Mr from light-scattering and from sedimentation and diffusion data is some 4 X 10(6)-5 X 10(6). Pronase digestion of the chains indicates that glycosylated sequences of Mr 0.3 X 10(6)-0.5 X 10(6) are interspersed with enzyme-susceptible non-glycosylated peptide sequences.

Sign in / Sign up

Export Citation Format

Share Document