EFFECT OF MAGNESIUM DEFICIENCY ON THE REGENERATION OF MAST CELLS AFTER TREATMENT WITH 48/80 IN RATS

1960 ◽  
Vol 38 (1) ◽  
pp. 585-589 ◽  
Author(s):  
Pierre Bois ◽  
Ernest H. Byrne ◽  
Leonard F. Bélanger
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Guinea Pig ◽  
Cell Population ◽  
Magnesium Deficiency ◽  
Guinea Pig Ileum ◽  
Deficient Diet ◽  
Cell Counts ◽  
Histamine Liberator ◽  
Mast Cell Population

The decrease in subcutaneous mast cell population observed in rats on a magnesium-deficient diet has been confirmed. Pretreatment with histamine liberator compound 48/80 accentuates the decrease in mast cell counts. This condition was apparently the result of defective regeneration. The decrease in the number of subcutaneous mast cells was accompanied by a parallel reduction of histamine as appreciated by the guinea pig ileum assay. The peripheral vasodilation, which is an early sign of magnesium deficiency, did not appear in rats pretreated with 48/80.

EFFECT OF MAGNESIUM DEFICIENCY ON THE REGENERATION OF MAST CELLS AFTER TREATMENT WITH 48/80 IN RATS

1960 ◽  
Vol 38 (6) ◽  
pp. 585-589 ◽  
Author(s):  
Pierre Bois ◽  
Ernest H. Byrne ◽  
Leonard F. Bélanger
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Guinea Pig ◽  
Cell Population ◽  
Magnesium Deficiency ◽  
Guinea Pig Ileum ◽  
Deficient Diet ◽  
Cell Counts ◽  
Histamine Liberator ◽  
Mast Cell Population

The decrease in subcutaneous mast cell population observed in rats on a magnesium-deficient diet has been confirmed. Pretreatment with histamine liberator compound 48/80 accentuates the decrease in mast cell counts. This condition was apparently the result of defective regeneration. The decrease in the number of subcutaneous mast cells was accompanied by a parallel reduction of histamine as appreciated by the guinea pig ileum assay. The peripheral vasodilation, which is an early sign of magnesium deficiency, did not appear in rats pretreated with 48/80.

Cerebral vasospasm: presence of mast cells in human cerebral arteries after aneurysm rupture

1981 ◽  
Vol 54 (6) ◽  
pp. 733-735 ◽  
Author(s):  
Luiz C. M. Faleiro ◽  
Conceição R. S. Machado ◽  
Amado Gripp ◽  
Rubens A. Resende ◽  
Pedro A. Rodrigues
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Population ◽  
Hydrolytic Enzymes ◽  
Aneurysm Rupture ◽  
Blue Staining ◽  
Control Group ◽  
Preliminary Note ◽  
Muscular Layer ◽  
Mast Cell Population

✓ Mast cells contain heparin, histamine, hydrolytic enzymes, and possibly serotonin in metachromatic cytoplasmic granules, and are not visualized in routine histological preparations. Special fixation, frozen sections, and toluidine blue staining are essential for counting the number of mast cells in tissue sections. Histological preparations for counting mast cells were made from arteries of the circle of Willis in persons who died after chest or abdominal trauma (control group) and in patients who had subarachnoid hemorrhage (SAH) after aneurysm rupture. The arteries were removed within 6 hours of death, taking care to avoid damage to their structure, and were immersed in the fixative solution. This preliminary note, reporting findings in only a few cases, is justified by the interesting discovery of a marked increase in mast cell population in the muscular layer of arteries after SAH. The series is small because of the difficulty in obtaining suitable material, since mast cells virtually disappear when autopsy is performed later than 6 hours after death. It is concluded from this study that there is an increase of mast cell population in cerebral arterial walls after SAH, mainly in the muscular layer, and that the number of mast cells is higher in arteries closer to the aneurysm.

scholarly journals Tryptase Profile of the Rat Skin Mast Cell Population During the Wound Healing

2021 ◽  
Vol 9 (4) ◽  
pp. 84-89
Author(s):  
V. V. Shishkina ◽  
S. V. Klochkova ◽  
N. T. Alexeeva ◽  
M. Yu. Soboleva ◽  
D. I. Esaulenko ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Population ◽  
Biological Activities ◽  
Repair Process ◽  
Fiber Formation ◽  
Control Group ◽  
Rat Skin ◽  
Wide Range ◽  
Mast Cell Population

Mast cells cyclically synthesize and excrete a wide range of biogenesis products with different biological activities into the extracellular matrix and are regulators of local homeostasis both in normal conditions and in pathology – inflammation, oncogenesis, etc. The relative specificity of classical histochemical methods for detecting mast cells in relation to chromogenic to substrates causes certain difficulties in the selective study of the components of the secretome of mast cells, for example, heparin, histamine, chymase or tryptase. Therefore, immunomorphological techniques have become very popular, which identify specific substrates and allow differentiation of the components of the mast cell secretome. Mediators produced by mast cells promote neoangiogenesis, fibrillogenesis and re-epithelialization during the repair process.The aim of our work was to study the tryptase profile of the mast cell population of rat skin during the wound processusing an original combined method of immunohistochemical staining.Material and methods. The experiment involved 12 Wistar rats divided into two groups – intact (n=6) and with the existing wound process of the skin in the withers (n=6). The tryptase profile of mast cells was assessed on the 7th day of the wound process in comparison with the control group.Results. The results obtained showed a significant increase in the number of tryptase-positive mast cells on the 7th day of the wound process in the skin against the background of a general increase in the population of mast cells. Intragranular tryptase reserve was significantly increased. In contrast to the control, where mast cells with single tryptase-positive granules dominated, during the wound process, cells of this type were practically not detected in the skin (43.69±2.9% and 8.55±0.9%). The content of tryptase-positive mast cells with complete filling of the cytoplasm in the control group and the group of animals with a wound process was 14.24±1.2% and 38.03±2.9%, respectively.Conclusion. Thus, when modeling a wound, an increase in tryptase synthesis is detected both in individual MCs and within the entire MC population. This fact indicates that mast cell proteases can become a potential therapeutic target for improving wound regeneration by correcting immunogenesis, inflammation and fiber formation.

Mast Cell Population of Lung of the Guinea Pig and other Tissues

Nature ◽  
1959 ◽  
Vol 184 (4693) ◽  
pp. 1154-1155 ◽  
Author(s):  
W. G. SMITH
Keyword(s):  
Mast Cell ◽  
Guinea Pig ◽  
Cell Population ◽  
Mast Cell Population

THE OXYTOCIN-INACTIVATING ACTIVITY OF THE RAT UTERUS

1972 ◽  
Vol 52 (3) ◽  
pp. 451-457
Author(s):  
M. N. BURJORJEE ◽  
U. MALHOTRA ◽  
R. R. CHAUDHURY
Keyword(s):  
Mast Cell ◽  
Cell Count ◽  
Cell Population ◽  
Intrauterine Devices ◽  
Rat Uterus ◽  
Cell Counts ◽  
Mast Cell Count ◽  
Series Of Experiments ◽  
Mast Cell Population ◽  
Intact Rats

SUMMARY The oxytocin-inactivating activity (OIA) of rat uterus homogenates was studied in intact animals and in animals with bilateral intrauterine devices (IUD). In another series of experiments the OIA of the rat uterus was related to the mast cell count of uteri from intact rats and from rats with bilateral intrauterine devices. The OIA of the homogenates was significantly higher at oestrus than at dioestrus. No such increase was observed in homogenates from rats at oestrus with bilateral IUD's. The IUD caused an increase in the mast cell population at oestrus and the increase in mast cell counts observed in uterine homogenates of intact rats at dioestrus was not observed in the presence of a device. No correlation between the OIA of uterine homogenates and the mast cell population was observed in animals with or without IUD at oestrus or dioestrus.

scholarly journals Flow Cytometry-Based Characterization of Mast Cells in Human Atherosclerosis

Cells ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 334 ◽  
Author(s):  
Kritikou ◽  
Depuydt ◽  
de Vries ◽  
Mulder ◽  
Govaert ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mast Cell ◽  
Mast Cells ◽  
Cell Population ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Atherosclerotic Plaques ◽  
Human Atherosclerosis ◽  
Mast Cell Population

The presence of mast cells in human atherosclerotic plaques has been associated with adverse cardiovascular events. Mast cell activation, through the classical antigen sensitized-IgE binding to their characteristic Fcε-receptor, causes the release of their cytoplasmic granules. These granules are filled with neutral proteases such as tryptase, but also with histamine and pro-inflammatory mediators. Mast cells accumulate in high numbers within human atherosclerotic tissue, particularly in the shoulder region of the plaque. These findings are largely based on immunohistochemistry, which does not allow for the extensive characterization of these mast cells and of the local mast cell activation mechanisms. In this study, we thus aimed to develop a new flow-cytometry based methodology in order to analyze mast cells in human atherosclerosis. We enzymatically digested 22 human plaque samples, collected after femoral and carotid endarterectomy surgery, after which we prepared a single cell suspension for flow cytometry. We were able to identify a specific mast cell population expressing both CD117 and the FcεR, and observed that most of the intraplaque mast cells were activated based on their CD63 protein expression. Furthermore, most of the activated mast cells had IgE fragments bound on their surface, while another fraction showed IgE-independent activation. In conclusion, we are able to distinguish a clear mast cell population in human atherosclerotic plaques, and this study establishes a strong relationship between the presence of IgE and the activation of mast cells in advanced atherosclerosis. Our data pave the way for potential therapeutic intervention through targeting IgE-mediated actions in human atherosclerosis.

Dietary Copper Deficiency Increases the Mast Cell Population of the Rat

1994 ◽  
Vol 207 (3) ◽  
pp. 274-277 ◽  
Author(s):  
D. A. Schuschke ◽  
J. T. Saari ◽  
C. A. West ◽  
F. N. Miller
Keyword(s):  
Mast Cell ◽  
Cell Population ◽  
Copper Deficiency ◽  
Dietary Copper ◽  
Mast Cell Population

Mass cells contribute to O3-induced epithelial damage and proliferation in nasal and bronchial airways of mice

1996 ◽  
Vol 80 (4) ◽  
pp. 1322-1330 ◽  
Author(s):  
M. Longphre ◽  
L. Y. Zhang ◽  
J. R. Harkema ◽  
S. R. Kleeberger
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Dna Synthesis ◽  
Measured Parameter ◽  
Epithelial Injury ◽  
Epithelial Damage ◽  
Proinflammatory Mediators ◽  
Cell Counts ◽  
Air Control

Ozone (O3) exposure produces inflammation in the airways of humans and animal models. However, the mechanism by which O3 affects these changes is uncertain. Mast cells are strategically located below the epithelium of the airways and are capable of releasing a number of proinflammatory mediators. We tested the hypothesis that mast cells contribute to inflammation, epithelial sloughing, and epithelial proliferation in the nasal and terminal bronchiolar murine airways after O3 exposure. Mast cell-sufficient (+/+), mast cell-deficient (W/Wv), and mast cell-repleted [bone marrow-transplanted (BMT) W/Wv] mice were exposed to 2 ppm O3 or filtered air for 3 h. Nasal and bronchoalveolar lavage fluids were collected 6 and 24 h after exposure. Differential cell counts and protein content of the lavage fluids were used as indicators of inflammation and permeability changes in the airways. O3-induced epithelial injury was assessed by light microscopy, and O3-induced DNA synthesis in airway epithelium was estimated by using a 5-bromo-2′-deoxyuridine-labeling index in the nasal and terminal bronchiolar epithelia. Relative to air control mice, O3 caused significant increases in inflammation, epithelial injury, and epithelial DNA synthesis in +/+ mice. There was no significant effect of O3 exposure on any measured parameter in the W/Wv mice. To further assess the role of mast cells in O3-induced epithelial damage, mast cells were restored in W/Wv mice by BMT from +/+ congeners. Relative to sham-transplanted W/Wv mice, O3 caused significant increases in epithelial damage and DNA synthesis as well as inflammatory indicators in BMT W/Wv mice. These observations are consistent with the hypothesis that mast cells significantly modulate the inflammatory and proliferative responses of the murine airways to O3.

Photoelectric Method for Quantitative Evaluation of Mast-Cell-Associated Enzyme Activity in Human Gingival Tissues

1970 ◽  
Vol 49 (3) ◽  
pp. 480-486
Author(s):  
F.M. Sorenson ◽  
J.S. Bennett ◽  
D. Fujita ◽  
F.R. Poindexter ◽  
W.B. Hall
Keyword(s):  
Mast Cell ◽  
Enzyme Activity ◽  
Mast Cells ◽  
Quantitative Evaluation ◽  
Photoelectric Method ◽  
Scanning Method ◽  
Cell Counts ◽  
Biologic Activity ◽  
Large Numbers ◽  
Human Gingiva

Simple counts of mast cells per unit of human gingiva are often difficult to interpret because of the large numbers and varying sizes and shapes of the counted structures. The relatively simple photoelectric scanning method described herein eliminates tedious counting procedures while providing a measure of the relative quantity of stainable mast cell granules within the area scanned. Thus, the method may provide a better estimate of the total biologic activity than would simple mast cell counts.

scholarly journals Quantitation of mast cell heparin by flow cytofluorometry.

1976 ◽  
Vol 24 (12) ◽  
pp. 1231-1238 ◽  
Author(s):  
L Enerbäck ◽  
G Berlin ◽  
I Svensson ◽  
I Rundquist
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Mixed Cell ◽  
Flow Cytofluorometry ◽  
Frequency Distributions ◽  
Cell Counts ◽  
Mixed Cell Population ◽  
Number Of Cells ◽  
Excellent Reproducibility

Mast cells can be automatically identified in a mixed cell population by flow cytofluorometry after Berberine sulphate staining. Volume specific counts of the total number of cells and number of mast cells, as well as frequency distributions of fluorescence intensities of mast cells, based on a large number of cells, can be rapidly obtained. Results obtained by microscope fluorometry of cells identified by phase contrast microscopy showviously published results it may be inferred that the fluorescence intensity of individual mast cells is proportional to mast cell heparin content. The automated cell counts correlated very well with manual hemocytometer counts. Both cell counts and the determination of mean mast cell fluorescence showed excellent reproducibility.

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