THE EFFECT OF INSULIN ADMINISTRATION ON ARTERIOVENOUS GLUCOSE DIFFERENCES IN THE ALLOXAN-DIABETIC DOG

1956 ◽  
Vol 34 (1) ◽  
pp. 763-767
Author(s):  
Doris E. Gray ◽  
H. A. DeLuca

It has been reported by others and confirmed by the present workers that following the intravenous administration of insulin in the normal animal, the glucose concentration in venous blood may exceed that of arterial blood giving rise to a negative arteriovenous difference. The simultaneous administration of glucose enhances this effect. The present investigators have extended this work to the alloxan-diabetic animal (dog) and have shown that large negative arteriovenous blood glucose differences are obtained following administration of insulin and glucose. These differences are too great and too consistent to allow the contention that negative arteriovenous differences are simply the result of analytical errors.

1956 ◽  
Vol 34 (4) ◽  
pp. 763-767
Author(s):  
Doris E. Gray ◽  
H. A. DeLuca

It has been reported by others and confirmed by the present workers that following the intravenous administration of insulin in the normal animal, the glucose concentration in venous blood may exceed that of arterial blood giving rise to a negative arteriovenous difference. The simultaneous administration of glucose enhances this effect. The present investigators have extended this work to the alloxan-diabetic animal (dog) and have shown that large negative arteriovenous blood glucose differences are obtained following administration of insulin and glucose. These differences are too great and too consistent to allow the contention that negative arteriovenous differences are simply the result of analytical errors.


2020 ◽  
Vol 17 (3) ◽  
pp. 6-16
Author(s):  
A. B. Naumov ◽  
Yu. S. Polushin ◽  
G. G. Khubulava ◽  
Yu. S. Аleksandrovich ◽  
S. P. Marchenko ◽  
...  

The objective: to identify laboratory markers of systemic perfusion in newborns with functional single ventricle on mechanical ventilation after surgical correction. Subjects and methods. Blood gas parameters were retrospectively analyzed in 52 newborns with congenital heart defects with univentricular hemodynamic after surgical correction. All samples were divided into three groups based on arterial blood saturation (SaO2): Group 1 – hypoxia (SaO2 ≤ 65%); Group 2 – normoxemia (SaO2 = 65-85%); Group 3 – hyperoxemia (SaO2 > 85%). Stroke volume and cardiac index were evaluated with echocardiography. The oxygen consumption and carbon metabolism were evaluated by arterial and venous blood gases. Results. The mixed central venous pO2 (PvO2) > 29.5 mm Hg, mixed central venous O2 (SvO2) > 54.5%, arteriovenous difference in saturation (Sa-vO2) < 15.8%, total oxygen content in venous blood (CvO2) > 119 ml/l, oxygen extraction ratio (O2ER) < 19% and the arteriovenous difference in partial pressure of carbon dioxide (dPCO2) < 5.4 mm Hg are cut off criteria for adequate systemic perfusion. PvO2 < 26 mm Hg, SvO2 < 44.5%, Sa-vO 2 > 27%, CvO2 < 88 ml/l, O2ER > 27.7%, dPCO2> 7.9 mm Hg have been associated with decreased systemic perfusion. The logistic regression model including combination of O2ER and dPCO2 predicts adequate systemic flow accuracy of 94.3% (sensitivity 87.5%, specificity 94.7%, p = 0.001). Graphics allow to adapt the mathematical model to clinical practice to verify systemic hypoperfusion in newborns with functional single ventricle. Conclusion: The following cut off parameters allow to assess systemic perfusion in newborns with functional single ventricle: PvO2, SvO2, CvO2, Sa-vO 2, O2ER, and dPCO2. The model for predicting the adequacy of systemic perfusion can be used as an effective tool to monitor hemodynamic status in newborns with functional single ventricle.


2002 ◽  
Vol 92 (4) ◽  
pp. 1677-1683 ◽  
Author(s):  
H. B. Nielsen ◽  
J. O. Clemmesen ◽  
C. Skak ◽  
P. Ott ◽  
N. H. Secher

We evaluated whether the increase in blood lactate with intense exercise is influenced by a low hepatosplanchnic blood flow as assessed by indocyanine green dye elimination and blood sampling from an artery and the hepatic vein in eight men. The hepatosplanchnic blood flow decreased from a resting value of 1.6 ± 0.1 to 0.7 ± 0.1 (SE) l/min during exercise. Yet the hepatosplanchnic O2uptake increased from 67 ± 3 to 93 ± 13 ml/min, and the output of glucose increased from 1.1 ± 0.1 to 2.1 ± 0.3 mmol/min ( P < 0.05). Even at the lowest hepatosplanchnic venous hemoglobin O2 saturation during exercise of 6%, the average concentration of glucose in arterial blood was maintained close to the resting level (5.2 ± 0.2 vs. 5.5 ± 0.2 mmol/l), whereas the difference between arterial and hepatic venous blood glucose increased to a maximum of 22 mmol/l. In arterial blood, the concentration of lactate increased from 1.1 ± 0.2 to 6.0 ± 1.0 mmol/l, and the hepatosplanchnic uptake of lactate was elevated from 0.4 ± 0.06 to 1.0 ± 0.05 mmol/min during exercise ( P < 0.05). However, when the hepatosplanchnic venous hemoglobin O2 saturation became low, the arterial and hepatosplanchnic venous blood lactate difference approached zero. Even with a marked reduction in its blood flow, exercise did not challenge the ability of the liver to maintain blood glucose homeostasis. However, it appeared that the contribution of the Cori cycle decreased, and the accumulation of lactate in blood became influenced by the reduced hepatosplanchnic blood flow.


1966 ◽  
Vol 16 (01/02) ◽  
pp. 032-037 ◽  
Author(s):  
D Ogston ◽  
C. M Ogston ◽  
N. B Bennett

Summary1. The concentration of the major components of the fibrinolytic enzyme system was compared in venous and arterial blood samples from male subjects.2. The plasminogen activator concentration was higher in venous blood and the arterio-venous difference increased as its concentration rose, but the ratio of the arterial to venous level remained constant.3. No arterio-venous difference was found for anti-urokinase activity, antiplasmin, plasminogen and fibrinogen.4. It is concluded that venous blood determinations of the components of the fibrinolytic enzyme system reflect satisfactorily arterial blood levels.


Author(s):  
A. G. Belova ◽  
E. V. Zimina ◽  
N. P. Simbirtsev

During a pathoanatomic autopsy, it is very important to correctly assess the color change of the organs. However, it is not always clear because the color depends on the spectrum of the incident light. There is also a subjective assessment of color. In addition, in animals with large amounts of circulating blood, for example, dogs, early imbibition occurs, which makes it difficult to assess the color of the organ and pathoanatomical diagnosis. We have proposed a simple and visual method of recognition of two pathological processes – inflammation and edema using colored filters. This technique also allows to accurately differentiate inflammation from postmortem imbibition, to recognize fibrin and hemorrhage well. Postmortem examination of different types of animals (predacious families of mustelids, canids, felids) was performed in accordance with Shore’s method in the prosectorium of the Pathonomy Department, K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology visual analysis – under various artificial lights (fluorescent lamps with banded spectrum and halogen lamps). In the red filter are well identified the pathological processes associated with the venous blood presence in the tissues (venous hyperemia and pulmonary edema). The focus of venous hyperemia or edema in the red filter looks like a dark zone, and tissues, where arterial bloods predominated, aren’t detected in red filter. In the yellow – green filter the inflammation is clearly detected: the zone is brightly red and surrounding tissues become dark. Red colour filters have rather narrow band of transmittance from 600 to 700 nm. Yellow-green have a width zone – from 500 to 700 nm, including both red, and yellow-green part of spectrum. Oxidized hemoglobin in red part of spectrum absorbs ten time weaker, has more high reflectivity and looks red. Surrounding tissues reflect the red rays, which incident on them also red. Therefore, the zone of edema, venous hyperemia and hemorrhaging, containing venous blood, are detected the dark spot, and inflammation zone merges with the red background. Oxidized hemoglobin in the red spectrum part absorbs ten time weaker than reduced hemoglobin, has high reflectivity of the red spectrum part and looks brightly red, surrounding tissues reflect yellow-green spectrum part and look green. Therefore, the zones of inflammation, active hyperemia and hemorrhaging, containing arterial blood, sharp contrast with green background and are clearly visible. Diagnoses made with the help of color filters are confirmed by histological studies.


2019 ◽  
pp. 203-206
Author(s):  
Mevlut Demir ◽  
◽  
Muslum Sahin ◽  
Ahmet Korkmaz ◽  
◽  
...  

Carbon monoxide intoxication occurs usually via inhalation of carbon monoxide that is emitted as a result of a fire, furnace, space heater, generator, motor vehicle. A 37-year-old male patient was admitted to the emergency department at about 5:00 a.m., with complaints of nausea, vomiting and headache. He was accompanied by his wife and children. His venous blood gas measures were: pH was 7.29, partial pressure of carbon dioxide (pCO2) was 42 mmHg, partial pressure of oxygen (pO2) was 28 mmHg, carboxyhemoglobin (COHb) was 12.7% (reference interval: 0.5%-2.5%) and oxygen saturation was 52.4%. Electrocardiogram (ECG) examination showed that the patient was not in sinus rhythm but had atrial fibrillation. After three hours the laboratory examination was repeated: Troponin was 1.2 pg/ml and in the arterial blood gas COHb was 3%. The examination of the findings on the monitor showed that the sinus rhythm was re-established. The repeated ECG examination confirmed the conversion to the sinus rhythm. He was monitored with the normobaric oxygen administration.


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