THE DEGRADATION OF DESOXYRIBONUCLEIC ACID DURING ALKALINE HYDROLYSIS

1956 ◽  
Vol 34 (1) ◽  
pp. 265-269 ◽  
Author(s):  
R. O. Hurst
Keyword(s):  
Acid Solution ◽  
Trichloroacetic Acid ◽  
Amino Nitrogen ◽  
Organic Phosphate ◽  
Alkaline Degradation ◽  
Desoxyribonucleic Acid ◽  
Trichloroacetic Acid Solution ◽  
Hydrolysis Of ◽  
Complete Formation ◽  
Hydrolysis Of Dna

The extent of alkaline degradation of desoxyribonucleic acid (DNA) was determined by following the formation of organic phosphate soluble in 10% trichloroacetic acid solution and the liberation of ammonia. Hydrolysis of DNA with 1 N sodium hydroxide at 100 °C. for four hours was required to effect complete formation of acid-soluble oligonucleotides and the ammonia liberated was equivalent to the estimated amino nitrogen content of the DNA.

THE DEGRADATION OF DESOXYRIBONUCLEIC ACID DURING ALKALINE HYDROLYSIS

1956 ◽  
Vol 34 (2) ◽  
pp. 265-269
Author(s):  
R. O. Hurst
Keyword(s):  
Acid Solution ◽  
Trichloroacetic Acid ◽  
Amino Nitrogen ◽  
Organic Phosphate ◽  
Alkaline Degradation ◽  
Desoxyribonucleic Acid ◽  
Trichloroacetic Acid Solution ◽  
Hydrolysis Of ◽  
Complete Formation ◽  
Hydrolysis Of Dna

The extent of alkaline degradation of desoxyribonucleic acid (DNA) was determined by following the formation of organic phosphate soluble in 10% trichloroacetic acid solution and the liberation of ammonia. Hydrolysis of DNA with 1 N sodium hydroxide at 100 °C. for four hours was required to effect complete formation of acid-soluble oligonucleotides and the ammonia liberated was equivalent to the estimated amino nitrogen content of the DNA.

scholarly journals Application of in situ digital holography to the study of the effect of a magnetic field on the anodic dissolution of iron in thichloroacetic acid

2006 ◽  
Vol 71 (1) ◽  
pp. 67-73 ◽  
Author(s):  
Xuegeng Yang ◽  
Shenhao Chen ◽  
Chao Wang ◽  
LI. Liang
Keyword(s):  
Magnetic Field ◽  
Acid Solution ◽  
Anodic Dissolution ◽  
Potentiodynamic Polarization ◽  
Trichloroacetic Acid ◽  
Digital Holography ◽  
Polarization Method ◽  
Trichloroacetic Acid Solution ◽  
The Magnetic Field

The effect of a magnetic field on the anodic dissolution of iron in 1.0 mol dm-3 trichloroacetic acid solution was studied by the potentiodynamic polarization method and in situ digital holography. It was found that the magnetohydrodynamic force increased the mass transport, which resulted in a faster anodic dissolution of iron. The effect of the magnetic field was analyzed by holograms and is discussed in terms of the magnetohydrodynamic force.

Spectrophotometric Determination of Vitamin A in Oils and Fats

1978 ◽  
Vol 61 (3) ◽  
pp. 753-755
Author(s):  
Teymour Kamangar ◽  
Ahmad B Fawzf
Keyword(s):  
Vitamin A ◽  
Acid Solution ◽  
Trichloroacetic Acid ◽  
Molar Absorptivity ◽  
Vitamin D2 ◽  
Cod Liver Oil ◽  
Trichloroacetic Acid Solution ◽  
Blue Reaction ◽  
Β Carotene

Abstract A spectrophotometric method for determining vitamin A based on interaction with 50% trichloroacetic acid solution in dichloromethane was developed. The blue reaction product had a maximum absorption at 620 nm and obeyed Beer's law over the concentration range of 0.5-5.0 μg retinol/ml solution. The molar absorptivity of the reaction product was 1.58 × 105. As much as 100 μg vitamin D2, and β-carotene up to 12 times the vitamin A concentration, did not interfere with the determination. The results obtained from the determination of vitamin A in cod liver oil and butter showed excellent agreement with the Carr-Price method, 43.013 (d).

scholarly journals Nucleic acid metabolism in the ruminant

1969 ◽  
Vol 23 (3) ◽  
pp. 671-682 ◽  
Author(s):  
A. B. Mcallan ◽  
R. H. Smith
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Acid Solution ◽  
Perchloric Acid ◽  
Trichloroacetic Acid ◽  
Acid Metabolism ◽  
Nucleic Acid Metabolism ◽  
Trichloroacetic Acid Solution ◽  
Rna And Dna

1. Procedures, based on those of Schmidt & Thannhauser (1945) and Schneider (1945), for the extraction and estimation of nucleic acids in bovine digesta were examined in detail.2. Final methods which were suitable for routine determination of RNA and DNA were essentially as follows. Digesta samples were extracted in the cold, first with a solution of trichloroacetic acid in ethanol, then with aqueous trichloroacetic acid solution and finally with lipid solvents. The dried residue was hydrolysed with alkali, purified by passage through a Dowex resin, and the RNA, in the form of mononucleotides, determined by U.V. absorption. DNA was determined separately in hot perchloric acid extracts of the original dried residue by colorimetric estimation of the deoxyribose content.

Evaluation of Mucociliary Function in Patients with Allergic Rhinitis Treated with Trichloroacetic Acid: Mucociliary Transit Time (Saccharin Time) Measurements

1995 ◽  
Vol 9 (6) ◽  
pp. 357-360 ◽  
Author(s):  
Kazuo Yao ◽  
Tetsuya Shitara ◽  
Hiro-omi Takahashi ◽  
Kouichiro Nishiyama ◽  
Yoshiaki Iguchi ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Acid Solution ◽  
Normal Control ◽  
Trichloroacetic Acid ◽  
Inferior Turbinate ◽  
Time Measurement ◽  
Ciliary Epithelium ◽  
Therapeutic Application ◽  
Trichloroacetic Acid Solution ◽  
Mucociliary Function

By means of the saccharin time measurement, the mucociliary function of the nasal mucosa of the inferior turbinate was evaluated in clinical cases with allergic rhinitis after the therapeutic application of trichloroacetic acid solution. It was found that the saccharin time (ST) tended to be prolonged within 1 year after the treatment, whereas it gradually became shortened to reach the level of normal control after 3 years. The result would indicate that the mucociliary function recovered correspondingly with regeneration of the ciliary epithelium.

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