SPECTROPHOTOMETRIC ANALYSIS OF NUCLEIC ACIDS IN PROTEIN SOLUTIONS AND IN CRUDE ORGAN EXTRACTS

1955 ◽  
Vol 33 (1) ◽  
pp. 1010-1017
Author(s):  
E. Annau
Keyword(s):  
Absorption Spectrum ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mouse Liver ◽  
Spectrophotometric Analysis ◽  
Protein Solutions ◽  
Spectrophotometric Procedure ◽  
Acid Protein ◽  
Calf Thymus ◽  
Nucleoprotein Complexes

A spectrophotometric procedure has been presented by which an absorption spectrum, essentially characteristic for nucleic acids, could be obtained from mixed nucleic acid protein solutions, and mouse liver extracts. To examine the efficiency of the method for nucleoprotein complexes, spectra from purified calf thymus nucleohiston were prepared showing the absorption curves of both of its components: nucleic acids and histon.

SPECTROPHOTOMETRIC ANALYSIS OF NUCLEIC ACIDS IN PROTEIN SOLUTIONS AND IN CRUDE ORGAN EXTRACTS

1955 ◽  
Vol 33 (6) ◽  
pp. 1010-1017 ◽  
Author(s):  
E. Annau
Keyword(s):  
Absorption Spectrum ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mouse Liver ◽  
Spectrophotometric Analysis ◽  
Protein Solutions ◽  
Spectrophotometric Procedure ◽  
Acid Protein ◽  
Calf Thymus ◽  
Nucleoprotein Complexes

A spectrophotometric procedure has been presented by which an absorption spectrum, essentially characteristic for nucleic acids, could be obtained from mixed nucleic acid protein solutions, and mouse liver extracts. To examine the efficiency of the method for nucleoprotein complexes, spectra from purified calf thymus nucleohiston were prepared showing the absorption curves of both of its components: nucleic acids and histon.

Snapshot blotting: The transfer of nucleic acids and nucleoprotein complexes from electrophoresis gels to EM grids

1992 ◽  
Vol 50 (1) ◽  
pp. 762-763
Author(s):  
Stephen D. Jett
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Binding ◽  
Mobility Shift ◽  
Carbon Coated ◽  
Nucleoprotein Complexes ◽  
Mobility Shift Assay ◽  
Protein Nucleic Acid ◽  
Gel Mobility Shift ◽  
Nucleic Acid Interactions

The electrophoresis gel mobility shift assay is a popular method for the study of protein-nucleic acid interactions. The binding of proteins to DNA is characterized by a reduction in the electrophoretic mobility of the nucleic acid. Binding affinity, stoichiometry, and kinetics can be obtained from such assays; however, it is often desirable to image the various species in the gel bands using TEM. Present methods for isolation of nucleoproteins from gel bands are inefficient and often destroy the native structure of the complexes. We have developed a technique, called “snapshot blotting,” by which nucleic acids and nucleoprotein complexes in electrophoresis gels can be electrophoretically transferred directly onto carbon-coated grids for TEM imaging.

scholarly journals Nucleic acid protein crystallography facilitated by selenium nucleic acids (SeNA)

2019 ◽  
Vol 75 (a1) ◽  
pp. a158-a158
Author(s):  
Zhen Huang ◽  
Andrey Kovalevsky ◽  
Qianwei Zhao ◽  
Lillian Hu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Protein Crystallography ◽  
Acid Protein

scholarly journals Helicase Mechanisms During Homologous Recombination inSaccharomyces cerevisiae

2019 ◽  
Vol 48 (1) ◽  
pp. 255-273 ◽  
Author(s):  
J. Brooks Crickard ◽  
Eric C. Greene
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleic Acid ◽  
Homologous Recombination ◽  
Nucleic Acids ◽  
Acid Metabolism ◽  
Model Organism ◽  
Nucleic Acid Metabolism ◽  
Repair Pathway ◽  
Unidirectional Movement ◽  
Nucleoprotein Complexes

Helicases are enzymes that move, manage, and manipulate nucleic acids. They can be subdivided into six super families and are required for all aspects of nucleic acid metabolism. In general, all helicases function by converting the chemical energy stored in the bond between the gamma and beta phosphates of adenosine triphosphate into mechanical work, which results in the unidirectional movement of the helicase protein along one strand of a nucleic acid. The results of this translocation activity can range from separation of strands within duplex nucleic acids to the physical remodeling or removal of nucleoprotein complexes. In this review, we focus on describing key helicases from the model organism Saccharomyces cerevisiae that contribute to the regulation of homologous recombination, which is an essential DNA repair pathway for fixing damaged chromosomes.

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