URINARY EXCRETION PRODUCTS OF MENADIONE (VITAMIN K3)

1954 ◽  
Vol 32 (1) ◽  
pp. 240-250 ◽  
Author(s):  
F. C. G. Hoskin ◽  
J. W. T. Spinks ◽  
L. B. Jaques
Keyword(s):  
Body Weight ◽  
Guinea Pig ◽  
Urinary Excretion ◽  
Absorption Spectra ◽  
Vitamin K ◽  
Paper Chromatography ◽  
Ultraviolet Absorption ◽  
Chemical Analyses ◽  
Vitamin K3 ◽  
Dosage Range

Radioactive vitamin K3 (2-methyl-C14-1,4-naphthoquinone) was administered to rats and the radioactive urinary products separated by paper chromatography and identified by ultraviolet absorption spectra and chemical analyses. Over the dosage range of 2.6 to 11.0 mgm. per kgm. of body weight, 2-methyl-C14-1,4-naphthoquinone was excreted, in order of decreasing relative concentrations as the diglucuronide (Product 1), the monosulphate (Product 2), and a third partially identified derivative of the vitamin (Product 3). Following the administration of vitamin K3, these products were excreted by normal rats, a dicoumarol-poisoned rat, and a guinea pig. The relative concentrations in the urine of Products 1 and 2 varied directly with the size of dose of vitamin K3. At these dosages, little or no vitamin K3 is excreted in urine as such.

URINARY EXCRETION PRODUCTS OF MENADIONE (VITAMIN K3)

1954 ◽  
Vol 32 (3) ◽  
pp. 240-250 ◽  
Author(s):  
F. C. G. Hoskin ◽  
J. W. T. Spinks ◽  
L. B. Jaques
Keyword(s):  
Body Weight ◽  
Guinea Pig ◽  
Urinary Excretion ◽  
Absorption Spectra ◽  
Vitamin K ◽  
Paper Chromatography ◽  
Ultraviolet Absorption ◽  
Chemical Analyses ◽  
Vitamin K3 ◽  
Dosage Range

Radioactive vitamin K3 (2-methyl-C14-1,4-naphthoquinone) was administered to rats and the radioactive urinary products separated by paper chromatography and identified by ultraviolet absorption spectra and chemical analyses. Over the dosage range of 2.6 to 11.0 mgm. per kgm. of body weight, 2-methyl-C14-1,4-naphthoquinone was excreted, in order of decreasing relative concentrations as the diglucuronide (Product 1), the monosulphate (Product 2), and a third partially identified derivative of the vitamin (Product 3). Following the administration of vitamin K3, these products were excreted by normal rats, a dicoumarol-poisoned rat, and a guinea pig. The relative concentrations in the urine of Products 1 and 2 varied directly with the size of dose of vitamin K3. At these dosages, little or no vitamin K3 is excreted in urine as such.

URINARY EXCRETION OF TRYPTOPHAN METABOLITES IN THE HEALTHY INFANT

PEDIATRICS ◽  
1962 ◽  
Vol 30 (4) ◽  
pp. 585-591
Author(s):  
Franco Vassella ◽  
Bo Hellström ◽  
Bo Wengle
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Paper Chromatography ◽  
Kynurenic Acid ◽  
Healthy Infant ◽  
Xanthurenic Acid ◽  
Two Dimensional ◽  
Healthy Infants ◽  
Tryptophan Metabolites ◽  
Hydroxyanthranilic Acid

Urinary excretion of tryptophan metabolites was studied qualitatively by two-dimensional paper chromatography in a group of 50 healthy infants with no tryptophan supplementation. Twenty-two infants of this group were given 100 mg of L-tryptophan per kilogram of body-weight, and the 24-hour urinary excretions of kynurenine, kynurenic acid, 3-hydroxykynurenine, 3-hydroxyanthranilic acid, and xanthurenic acid were estimated by quantitative paper chromatography. A high excretion of kynurenine was found to be a distinguishing feature. Various possibilities to explain this difference as compared to adults are discussed.

scholarly journals Evaluation of Vitamin K3 Feed Additive for Prevention of Sweet Clover Disease

1989 ◽  
Vol 1 (2) ◽  
pp. 116-119 ◽  
Author(s):  
Howard H. Casper ◽  
Arnold D. Alstad ◽  
David B. Tacke ◽  
Ladon J. Johnson ◽  
W. Eugene Lloyd
Keyword(s):  
Body Weight ◽  
Vitamin K ◽  
Feed Additive ◽  
Sweet Clover ◽  
Vitamin K3

Sweet clover poisoning in cattle is caused by an anticoagulant (dicumarol) that is formed in moldy sweet clover hay. Previous experiments with vitamin K3 and vitamin K3 in therapy trials indicated that vitamin K3 was effective in reducing prothrombin times but vitamin K3 was not. As a possible alternative in the use of toxic sweet clover hays, vitamin K3 was evaluated to see if it would prevent hemorrhagic crises when fed to cattle consuming toxic sweet clover hay. Vitamin K3 levels of 0, 0.45, 4.5, 11, and 45 mg/kg body weight/day were fed to 173-235-kg steers consuming toxic (40–50 ppm dicumarol) sweet clover. The 45-mg K3/kg/day supplement was not palatable and had to be discontinued. The 0.45, 4.5, and 11-mg K3/kg/day supplements did not significantly reduce the prothrombin times as compared to the 0-mg K3/kg/day group.

SEPARATION OF ENDOMYCINS A AND B, AND THEIR IDENTIFICATION AS MEMBERS OF THE POLYENE GROUPS OF ANTIFUNGAL ANTIBIOTICS

1957 ◽  
Vol 35 (12) ◽  
pp. 1461-1466 ◽  
Author(s):  
L. C. Vining ◽  
W. A. Taber
Keyword(s):  
Absorption Spectra ◽  
Paper Chromatography ◽  
Ultraviolet Absorption ◽  
Countercurrent Distribution ◽  
Double Bonds ◽  
Active Components ◽  
Streptomyces Species ◽  
Antifungal Antibiotics

An unidentified Streptomyces species isolated from platings of "honey dew" of the fungus Clavicepspurpurea produced two antifungal antibiotics which were shown by paper chromatography to be identical with the two active components of endomycin, and also with helixins A and B. An examination of the ultraviolet absorption spectra of the three complexes showed maxima at wavelengths associated with two unsaturated systems containing four and six conjugated double bonds respectively. The two unsaturated components were separated by countercurrent distribution and shown to correspond to the two active fractions. The tetraene has been named endomycin A, and the hexaene endomycin B. Flavacid, a polyene complex also containing tetraene and hexaene components, is not identical with endomycin.

scholarly journals The uptake of D-araboascorbic acid (D-isoascorbic acid) by guinea-pig tissues

1969 ◽  
Vol 23 (2) ◽  
pp. 211-216 ◽  
Author(s):  
R. E. Hughes ◽  
R. J. Hurley
Keyword(s):  
Ascorbic Acid ◽  
Body Weight ◽  
Guinea Pig ◽  
Paper Chromatography ◽  
Guinea Pigs ◽  
Intestinal Tract ◽  
No Conversion ◽  
Isoascorbic Acid ◽  
Gastro Intestinal Tract ◽  
Oral Supplement

1. Guinea-pigs on a low, controlled intake of ascorbic acid (0.3 mg/100 g body-weight) were given daily an oral supplement of L-ascorbic acid (AA) or of D-isoascorbic acid (isoAA) (both 1.5 mg/100 g body-weight). The AA was deposited in the tissues, the isoAA was not.2. Intramuscularly administered isoAA was deposited in the tissues but not to the same extent as equivalent amounts of intramuscularly administered AA.3. Paper chromatography confirmed that intramuscularly administered isoAA was deposited in the tissues as isoAA, i.e. there was no conversion into AA.4. It is concluded that in guinea-pigs (a)AA is absorbed from the gastro-intestinal tract much more readily than isoAA and (b)AA is either more readily abstracted from the blood or is more readily retained by the tissues or both, than is isoAA.

Tryptophan-Niacin Metabolism in Rat with Puromycin Aminonucleoside-Induced Nephrosis

2006 ◽  
Vol 76 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Yukari Egashira ◽  
Shin Nagaki ◽  
Hiroo Sanada
Keyword(s):  
Body Weight ◽  
Urinary Excretion ◽  
Enzyme Activities ◽  
Treated Group ◽  
Control Group ◽  
Puromycin Aminonucleoside ◽  
Low Level ◽  
Key Enzyme

We investigated the change of tryptophan-niacin metabolism in rats with puromycin aminonucleoside PAN-induced nephrosis, the mechanisms responsible for their change of urinary excretion of nicotinamide and its metabolites, and the role of the kidney in tryptophan-niacin conversion. PAN-treated rats were intraperitoneally injected once with a 1.0% (w/v) solution of PAN at a dose of 100 mg/kg body weight. The collection of 24-hour urine was conducted 8 days after PAN injection. Daily urinary excretion of nicotinamide and its metabolites, liver and blood NAD, and key enzyme activities of tryptophan-niacin metabolism were determined. In PAN-treated rats, the sum of urinary excretion of nicotinamide and its metabolites was significantly lower compared with controls. The kidneyα-amino-β-carboxymuconate-ε-semialdehyde decarboxylase (ACMSD) activity in the PAN-treated group was significantly decreased by 50%, compared with the control group. Although kidney ACMSD activity was reduced, the conversion of tryptophan to niacin tended to be lower in the PAN-treated rats. A decrease in urinary excretion of niacin and the conversion of tryptophan to niacin in nephrotic rats may contribute to a low level of blood tryptophan. The role of kidney ACMSD activity may be minimal concerning tryptophan-niacin conversion under this experimental condition.

ÉTUDE DE L'ACTIVITÉ BIOLOGIQUE IN VITRO DES HORMONES GONADOTROPES

1960 ◽  
Vol XXXIII (III) ◽  
pp. 444-450 ◽  
Author(s):  
Maria de la Luz Suarez Soto ◽  
Jean Legault Démare
Keyword(s):  
Paper Chromatography ◽  
Incubation Medium ◽  
Ultraviolet Absorption ◽  
Rat Ovary

ABSTRACT Serum gonadotrophin (PMS) when added to the incubation medium of rat ovary slices increases the amount of Δ4-3-ketosteroids produced. This enhancement is proportional to the logarithm of dose. The ketosteroids were determined by their ultraviolet absorption; paper chromatography has shown that only androst-4-en-3,17-dione is present.

FAR ULTRAVIOLET ABSORPTION SPECTRA OF N3AND N2+GENERATED BY ELECTRONS IMPACTING GASEOUS N2

2013 ◽  
Vol 779 (1) ◽  
pp. 40 ◽  
Author(s):  
Yu-Jong Wu ◽  
Hui-Fen Chen ◽  
Shiang-Jiun Chuang ◽  
Tzu-Ping Huang
Keyword(s):  
Absorption Spectra ◽  
Ultraviolet Absorption ◽  
Far Ultraviolet
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