Radioprotective effect of Curcuma longa extract on γ-irradiation-induced oxidative stress in rats

2012 ◽  
Vol 90 (4) ◽  
pp. 415-423 ◽  
Author(s):  
Ahmed S. Nada ◽  
Asrar M. Hawas ◽  
Nour El-Din Amin ◽  
Magdy M. Elnashar ◽  
Zakaria Y. Abd Elmageed
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Antioxidant Status ◽  
Curcuma Longa ◽  
Radioprotective Effect ◽  
Male Rats ◽  
Sublethal Dose ◽  
Γ Irradiation ◽  
Protein Levels ◽  
Radiation Induced

This study was conducted to evaluate the modulatory effect of aqueous extract of Curcuma longa (L.) against γ-irradiation (GR), which induces biochemical disorders in male rats. The sublethal dose of GR was determined in primary hepatocytes. Also, the effect of C. longa extract was examined for its activity against GR. In rats, C. longa extract was administered daily (200 mg/kg body mass) for 21 days before, and 7 days after GR exposure (6.5 Gy). The lipid profile and antioxidant status, as well as levels of transaminases, interleukin-6 (IL-6), and tumour necrosis factor α (TNFα) were assessed. The results showed that in hepatocytes, the aqueous extract exhibited radioprotective activity against exposure to GR. Exposure of untreated rats to GR resulted in transaminase disorders, lipid abnormalities, elevation of lipid peroxidation, trace element alterations, release of IL-6 and TNF, and decrease in glutathione and protein level of superoxide dismutase-1 (SOD-1) and peroxiredoxin-1 (PRDX-1). However, treatment of rats with this extract before and after GR exposure improved antioxidant status and minimized the radiation-induced increase in inflammatory cytokines. Changes occurred in the tissue levels of trace elements, and the protein levels of SOD-1 and PRDX-1 were also modulated by C. longa extract. Overall, C. longa exerted a beneficial radioprotective effect against radiation-induced oxidative stress in male rats by alleviating pathological disorders and modulating antioxidant enzymes.

Radioprotective effect of diethylcarbamazine on radiation-induced acute lung injury and oxidative stress in mice

2019 ◽  
Vol 52 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Soghra Farzipour ◽  
Fereshteh Talebpour Amiri ◽  
Ehsan Mihandoust ◽  
Fatemeh Shaki ◽  
Zohreh Noaparast ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Radioprotective Effect ◽  
Radiation Induced ◽  
And Oxidative Stress

Oral administration of Centella asiatica (L.) Urb leave aqueous extract ameliorates cerebral oxidative stress, inflammation, and apoptosis in male rats with type-2 diabetes

2020 ◽  
Vol 28 (6) ◽  
pp. 1599-1622
Author(s):  
Nelli Giribabu ◽  
Kamarulzaman Karim ◽  
Eswar Kumar Kilari ◽  
Srinivasa Rao Nelli ◽  
Naguib Salleh
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Centella Asiatica ◽  
Male Rats

scholarly journals Attenuation of Oxidative Stress-Associated Hematological Alterations and Hepatic Injury Induced by D-Dalactose or γ-Irradiation Using β-Hydroxybutyrate in Male Rats

2021 ◽  
Author(s):  
Mahmoud El Sayed Habieb ◽  
Marwa Abd El Hameed Mohamed ◽  
Doaa Mohamed ElGamal ◽  
Asrar Mohamed Hawas ◽  
Tarek Mohamed Mohamed
Keyword(s):  
Oxidative Stress ◽  
Hepatic Injury ◽  
Complete Blood Count ◽  
G6pd Activity ◽  
Male Rats ◽  
Protective Agent ◽  
Γ Irradiation ◽  
Sod Activity ◽  
Irradiation Group ◽  
Hematological Alterations

Abstract This work aims to investigate the possible inhibitory action of β-hydroxybutyrate (βOHB) against hematological alterations and hepatic injury associated with oxidative stress caused by D-galactose or γ-irradiation in rats. Six groups of male rats were used as the control, irradiated group (5 Gy), D-galactose (150 mg/kg b.wt), β-hydroxybutyrate (72.8 mg/kg b.wt), γ-irradiation plus βOHB, and D-galactose plus βOHB. Complete blood count and glucose-6-phosphate-dehydrogenase (G6PD) activity were determined in whole-blood samples. In addition, the hepatic malondialdehyde (MDA), nitric oxide (NO), and glutathione (GSH) levels and the superoxide dismutase (SOD) activity were evaluated. Moreover, certain elements were measured in liver tissue (iron (Fe), copper (Cu), and zinc (Zn)). The G6PD activity significantly diminished post exposure to D-galactose or γ-irradiation. In the βOHB, D-galactose, or γ-irradiation groups, liver MDA levels and SOD activity were significantly increased. Meanwhile, NO and GSH levels were significantly increased relative to normal control levels in the γ-irradiation group. The findings showed that βOHB alleviated hematological alterations, enhanced the altered biochemical indices, and modulated the change in Cu, Fe, and Zn elements in D-galactose or γ-irradiation group. These results highlight the role of βOHB as a powerful protective agent against hematological alterations and liver impairment by reducing G6PD-mediated oxidative stress and controlling the measured elements.

scholarly journals In vivo evidences of Curcuma longa on oxidative stress in STZ-induced diabetes on sperm parameters in male wistar rats

2017 ◽  
Vol 6 (3) ◽  
pp. 1045-1051
Author(s):  
A.J. Olanrewaju ◽  
S.Y. Olatunji ◽  
J.O. Owolabi ◽  
A.T. Oluwatosin ◽  
W.C. Amaechi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sperm Quality ◽  
Curcuma Longa ◽  
Diabetic Control ◽  
Positive Control ◽  
Sperm Parameters ◽  
Male Rats ◽  
Control Group ◽  
Alternative Source

Several conditions such as chemotherapy and toxins can interfere with spermatogenesis and reduce sperm quality and production. In the case where natural antioxidant response cannot manage oxidative stress and free radical overload, oxidative damage occurs and this begins the genesis of many diseases of which diabetes is one of them. This has awakened the interest of researchers to the use of an alternative source of medicine and herbal medicine. Medicinal use of Curcuma longa dates back to ancient China and India; its constituents are stated to have anti-hepatotoxic, anti-inflammatory, stimulant, and antioxidant and used since ancient time as medicinal and nutritive origins knowing to possess androgenic activities and have well effect in diseases treatment in more countries world-wide. As an antioxidant Curcuma longa possible has a useful effect on spermatogenesis and sperm parameters. Wistar male rats (n=24) were allocated into six groups, positive control (n=4), diabetic control (n=4) and experimental groups (n=20), that subdivided into groups of 4 that received treatment of Curcuma longa rhizome powder with or without STZ-induced diabetes in the dosages (25 and 100mg/kg/day) for 21 consecutive days. In twenty-second day, the testes were removed and semen was collected from epididymis and prepared for analysis. The percentage of sperm viability and motility in the treatment groups increased mildly with a significance of: (p<0.05) in comparison to control group and with the diabetic group being critically lower than those in control group. This suggested that Curcuma longa may be promising in enhancing sperm health parameters.Keywords: Turmeric, oxidative stress, semen, STZ-induced diabetes, antioxidant

Melatonin Modulates Regulation of NOX2 and NOX4 Following Irradiation in the Lung

2019 ◽  
Vol 14 (3) ◽  
pp. 224-231 ◽  
Author(s):  
Masoud Najafi ◽  
Alireza Shirazi ◽  
Elahe Motevaseli ◽  
Ghazale Geraily ◽  
Peyman Amini ◽  
...  
Keyword(s):  
Gamma Rays ◽  
Continuous Production ◽  
Male Rats ◽  
Whole Body ◽  
Oxygen Species ◽  
Local Irradiation ◽  
Nadph Oxidases ◽  
Melatonin Treatment ◽  
Protein Levels ◽  
Radiation Induced

Background: Exposure to ionizing radiation may lead to chronic upregulation of inflammatory mediators and pro-oxidant enzymes, which give rise to continuous production of reactive oxygen species (ROS). NADPH oxidases are among the most important ROS producing enzymes. Their upregulation is associated with DNA damage and genomic instability. In the present study, we sought to determine the expressions of NADPH oxidases; NOX2 and NOX4, in rat’s lung following whole body or pelvis irradiation. In addition, we evaluated the protective effect of melatonin on the expressions of NOX2 and NOX4, as well as oxidative DNA injury. Materials and Methods: 35 male rats were divided into 7 groups, G1: control; G2: melatonin (100 mg/kg) treatment; G3: whole body irradiation (2 Gy); G4: melatonin plus whole body irradiation; G5: local irradiation to pelvis area; G6: melatonin treatment plus 2 Gy gamma rays to pelvis area; G7: scatter group. All the rats were sacrificed after 24 h. afterwards, the expressions of TGFβR1, Smad2, NF- κB, NOX2 and NOX4 were detected using real-time PCR. Also, the level of 8-OHdG was detected by ELISA, and NOX2 and NOX4 protein levels were detected by western blot. Results: Whole body irradiation led to the upregulation of all genes, while local pelvis irradiation caused upregulation of TGFβR1, NF-κB, NOX2 and NOX4, as well as protein levels of NOX2 and NOX4. Treatment with melatonin reduced the expressions of these genes and also alleviated oxidative injury in both targeted and non-targeted lung tissues. Results also showed no significant reduction for NOX2 and NOX4 in bystander tissues following melatonin treatment. Conclusion: It is possible that upregulation of NOX2 and NOX4 is involved in radiation-induced targeted and non-targeted lung injury. Melatonin may reduce oxidative stress following upregulation of these enzymes in directly irradiated lung tissues but not for bystander.

scholarly journals THE POTENTIAL OF HIBISCUS SABDARIFFA LINN. (ROSELLE) POLYPHENOL-RICH EXTRACT AS A CARDIOPROTECTIVE AGENT IN MYOCARDIAL INFARCTION MODEL

2019 ◽  
Vol 81 (5) ◽  
Author(s):  
Siti Balkis Budin ◽  
Nor Anita Sharifuddin ◽  
Fatin Farhana Jubaidi ◽  
Satirah Zainalabidin
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Normal Saline ◽  
Antioxidant Status ◽  
Histological Study ◽  
Cardiac Injury ◽  
Male Rats ◽  
Control Group ◽  
Hibiscus Sabdariffa ◽  
Significant Difference

Myocardial infarction (MI) is a common cause of death due to interrupted blood supply to the heart. Roselle calyx (Hibiscus sabdariffa Linn.) is rich in polyphenols and has a potential in alleviating the risk of cardiovascular disease through its antioxidant activity. This study was aimed to investigate the effects of Hibiscus sabdariffa Linn. or roselle polyphenol-rich extract (HPE) supplementation in oxidative stress and cardiac injury biomarkers as well as cardiac histological changes following isoprenaline (ISO)-induced myocardial infarction (MI). Twenty-one male rats were randomly divided into three groups: control, MI, and HPE+MI. Normal saline or HPE (20 mg/kg BW) was given to MI and HPE+MI groups, respectively, for 14 consecutive days via force feeding. On the 15th and 16th day, 85 mg/kg body weight of ISO was administered subcutaneously to induce MI. Control group was only given normal saline throughout this 16-day duration of study. The results showed that HPE reduced the oxidative stress markers malondialdehyde (MDA) and nitrite oxide (NO) in HPE+MI group when compared with MI group (p<0.05) while increased reduced glutathione (GSH) level reflexes the improvement in antioxidant status. Cardiac injury biomarkers analysis showed no significant difference in HPE+MI group when compared to MI group. Histological study showed that HPE managed to reduce cardiac muscle fibre damage and infiltration of inflammatory cells in ISO-induced MI rats. In conclusion, HPE has the potential in protecting the heart against ISO-induced MI by reducing the oxidative stress and increasing antioxidant status. 

scholarly journals Aqueous Extract ofPhyllanthus niruriLeaves DisplaysIn VitroAntioxidant Activity and Prevents the Elevation of Oxidative Stress in the Kidney of Streptozotocin-Induced Diabetic Male Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Nelli Giribabu ◽  
Pasupuleti Visweswara Rao ◽  
Korla Praveen Kumar ◽  
Sekaran Muniandy ◽  
Somesula Swapna Rekha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Rat Kidney ◽  
Male Rats ◽  
Diabetic Rat ◽  
Activity Levels ◽  
Leaf Aqueous Extract ◽  
Gpx Activity

P. nirurihas been reported to possess antidiabetic and kidney protective effects. In the present study, the phytochemical constituents andin vitroantioxidant activity ofP. nirurileaf aqueous extract were investigated together with its effect on oxidative stress and antioxidant enzymes levels in diabetic rat kidney.Results. Treatment of diabetic male rats withP. nirurileaf aqueous extract (200 and 400 mg/kg) for 28 consecutive days prevents the increase in the amount of lipid peroxidation (LPO) product, malondialdehyde (MDA), and the diminution of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity levels in the kidney of diabetic rats. The amount of LPO showed strong negative correlation with SOD, CAT, and GPx activity levels.P. nirurileaf aqueous extract exhibitsin vitroantioxidant activity with IC50slightly lower than ascorbic acid. Phytochemical screening of plant extract indicates the presence of polyphenols.Conclusion.P. nirurileaf extract protects the kidney from oxidative stress induced by diabetes.

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