Stretch induced accumulation of total Ca and Na in cytosol and nucleus: a comparison between cardiac trabeculae and isolated myocytesThis paper is one of a selection of papers published in this Special Issue, entitled The Nucleus: A Cell Within A Cell.

2006 ◽  
Vol 84 (3-4) ◽  
pp. 487-498 ◽  
Author(s):  
J. Wilhelm ◽  
D. Kondratev ◽  
A. Christ ◽  
M.F. Gallitelli
Keyword(s):  
Mechanical Stimulus ◽  
Cationic Current ◽  
A Cell ◽  
Rat Myocytes ◽  
End To End ◽  
The Difference ◽  
Ventricular Trabeculae ◽  
Nonselective Cationic Current ◽  
Selection Of ◽  
Total Sodium

By means of electron probe microanalysis (EPMA), we quantified changes in total sodium [Na] and calcium [Ca] concentration owing to the following: (i) local axial stretch (LAS) of isolated rat myocytes and (ii) end-to-end stretch (ETES) of rat ventricular trabeculae. For LAS, the distance between patch pipette and a cell-attached stylus was increased by maximally 20%; this activated a nonselective cationic current ISAC of approximately –0.5 nA, which was blocked by streptomycin. Trabeculae were stretched end-to-end from 85% Lmax to Lmax. Stretch increased cytosolic [Na]total by 34% in isolated myocytes (p < 0.001) and by 43% in trabeculae (p < 0.001). The increment in nuclear [Na]total was 21% in myocytes (p < 0.01) and 20% in trabeculae (p < 0.001). Stretch increased [Ca]total in isolated myocytes, in both cytosol (from 0.63 ± 0.09 to 1.09 ± 0.20 mmol/L, p < 0.05) and nucleus (from 0.33 ± 0.05 to 0.64 ± 0.13 mmol/L, p < 0.05). In trabeculae, the stretch-induced increment of 51% in cytosolic [Ca]total remained nonsignificant (p < 0.15). In the nucleus, [Ca]total did not change. We interpret the difference of stretch on nuclear calcium in myocytes vs. trabeculae with the assumption that LAS, but not ETES, produces shear-stress components that translate the mechanical stimulus deeply into the cell where it may modulate [Ca]total by signals independent of ISAC.

COMBINATORIAL POLYNOMIALLY COMPUTABLE CHARACTERISTICS OF SUBSTITUTIONS AND THEIR PROPERTIES

2020 ◽  
Vol 7 (2) ◽  
pp. 34-41
Author(s):  
VLADIMIR NIKONOV ◽  
◽  
ANTON ZOBOV ◽  
Keyword(s):  
Mathematical Expectation ◽  
Point Of View ◽  
Small Range ◽  
Computational Point ◽  
Wide Range ◽  
Bijective Function ◽  
The Difference ◽  
Element Base ◽  
Selection Of

The construction and selection of a suitable bijective function, that is, substitution, is now becoming an important applied task, particularly for building block encryption systems. Many articles have suggested using different approaches to determining the quality of substitution, but most of them are highly computationally complex. The solution of this problem will significantly expand the range of methods for constructing and analyzing scheme in information protection systems. The purpose of research is to find easily measurable characteristics of substitutions, allowing to evaluate their quality, and also measures of the proximity of a particular substitutions to a random one, or its distance from it. For this purpose, several characteristics were proposed in this work: difference and polynomial, and their mathematical expectation was found, as well as variance for the difference characteristic. This allows us to make a conclusion about its quality by comparing the result of calculating the characteristic for a particular substitution with the calculated mathematical expectation. From a computational point of view, the thesises of the article are of exceptional interest due to the simplicity of the algorithm for quantifying the quality of bijective function substitutions. By its nature, the operation of calculating the difference characteristic carries out a simple summation of integer terms in a fixed and small range. Such an operation, both in the modern and in the prospective element base, is embedded in the logic of a wide range of functional elements, especially when implementing computational actions in the optical range, or on other carriers related to the field of nanotechnology.

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

2019 ◽  
pp. 27-33
Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov
Keyword(s):  
Phage Display ◽  
Selection Procedure ◽  
Free System ◽  
Phage Displays ◽  
High Affinity ◽  
Binding Domains ◽  
A Cell ◽  
Vhh Antibodies ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.

scholarly journals Age-dependency in mortality of Finnish family caregivers: a nationwide register-based study

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
T M Mikkola ◽  
H Kautiainen ◽  
M Mänty ◽  
M B von Bonsdorff ◽  
T Kröger ◽  
...  
Keyword(s):  
General Population ◽  
Family Caregivers ◽  
Family Caregiver ◽  
Causes Of Death ◽  
Lower Mortality ◽  
All Cause Mortality ◽  
The Difference ◽  
Caregiver Role ◽  
Selection Of

Abstract Purpose Mortality appears to be lower in family caregivers than in the general population. However, there is lack of knowledge whether the difference in mortality between family caregivers and the general population is dependent on age. The purpose of this study was to analyze all-cause mortality in relation to age in family caregivers and to study their cause-specific mortality using data from multiple Finnish national registers. Methods The data included all individuals, who received family caregiver's allowance in Finland in 2012 (n = 42 256, mean age 67 years, 71% women) and a control population matched for age, sex, and municipality of residence (n = 83 618). Information on dates and causes of death between 2012 and 2017 were obtained from the Finnish Causes of Death Register. Flexible parametric survival modeling and competing risk regression adjusted for socioeconomic status were used. Results The total follow-up time was 717 877 person-years. Family caregivers had lower all-cause mortality than the controls over the follow-up (8.1% vs. 11.6%) both among women (hazard ratio [HR]: 0.64, 95% CI: 0.61-0.68) and men (HR: 0.73, 95% CI: 0.70-0.77). Younger adult caregivers had equal or only slightly lower mortality than their controls, but after age 60, the difference increased markedly resulting in over 10% lower mortality in favor of the caregivers in the oldest age groups. Caregivers had lower mortality for all the causes of death studied, namely cardiovascular, cancer, neurological, external, respiratory, gastrointestinal and dementia than the controls. Of these, the lowest was the risk for dementia (subhazard ratio=0.29, 95%CI: 0.25-0.34). Conclusions Older family caregivers have lower mortality than the age-matched controls from the general population while younger caregivers have similar mortality to their peers. This age-dependent advantage in mortality is likely to reflect selection of healthier individuals into the family caregiver role. Key messages The difference in mortality between family caregivers and the age-matched general population varies considerably with age. Advantage in mortality observed in family caregiver studies is likely to reflect the selection of healthier individuals into the caregiver role, which underestimates the adverse effects of caregiving.

scholarly journals A microfluidic device enabling drug resistance analysis of leukemia cells via coupled dielectrophoretic detection and impedimetric counting

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yağmur Demircan Yalçın ◽  
Taylan Berkin Töral ◽  
Sertan Sukas ◽  
Ender Yıldırım ◽  
Özge Zorlu ◽  
...  
Keyword(s):  
Drug Resistance ◽  
K562 Cells ◽  
Leukemia Cells ◽  
Drug Resistant ◽  
Wild Type ◽  
Gene Expressions ◽  
Before And After ◽  
The Difference ◽  
Selection Of ◽  
Resistant Cells

AbstractWe report the development of a lab-on-a-chip system, that facilitates coupled dielectrophoretic detection (DEP-D) and impedimetric counting (IM-C), for investigating drug resistance in K562 and CCRF-CEM leukemia cells without (immuno) labeling. Two IM-C units were placed upstream and downstream of the DEP-D unit for enumeration, respectively, before and after the cells were treated in DEP-D unit, where the difference in cell count gave the total number of trapped cells based on their DEP characteristics. Conductivity of the running buffer was matched the conductivity of cytoplasm of wild type K562 and CCRF-CEM cells. Results showed that DEP responses of drug resistant and wild type K562 cells were statistically discriminative (at p = 0.05 level) at 200 mS/m buffer conductivity and at 8.6 MHz working frequency of DEP-D unit. For CCRF-CEM cells, conductivity and frequency values were 160 mS/m and 6.2 MHz, respectively. Our approach enabled discrimination of resistant cells in a group by setting up a threshold provided by the conductivity of running buffer. Subsequent selection of drug resistant cells can be applied to investigate variations in gene expressions and occurrence of mutations related to drug resistance.

scholarly journals Weightage Effect during Back-Calculation of Rock-Mass Quality from the Installed Tunnel Support in Rock-Mass Rating and Tunneling Quality Index System

2019 ◽  
Vol 9 (10) ◽  
pp. 2065 ◽  
Author(s):  
Jonguk Kim ◽  
Hafeezur Rehman ◽  
Wahid Ali ◽  
Abdul Muntaqim Naji ◽  
Hankyu Yoo
Keyword(s):  
Rock Mass ◽  
Quality Index ◽  
Rock Bolt ◽  
Rock Mass Rating ◽  
Rock Mass Quality ◽  
Back Calculation ◽  
The Difference ◽  
Bolt Spacing ◽  
Q System ◽  
Selection Of

In extensively used empirical rock-mass classification systems, the rock-mass rating (RMR) and tunneling quality index (Q) system, rock-mass quality, and tunnel span are used for the selection of rock bolt length and spacing and shotcrete thickness. In both systems, the rock bolt spacing and shotcrete thickness selection are based on the same principle, which is used for the back-calculation of the rock-mass quality. For back-calculation, there is no criterion for the selection of rock-bolt-spacing-based rock-mass quality weightage and shotcrete thickness along with tunnel-span-based rock-mass quality weightage. To determine this weightage effect during the back-calculation, five weightage cases are selected, explained through example, and applied using published data. In the RMR system, the weightage effect is expressed in terms of the difference between the calculated and back-calculated rock-mass quality in the two versions of RMR. In the Q system, the weightage effect is presented in plots of stress reduction factor versus relative block size. The results show that the weightage effect during back-calculation not only depends on the difference in rock-bolt-spacing-based rock-mass quality and shotcrete along with tunnel-span-based rock-mass quality, but also on their corresponding values.

Effect of Bilingualism on Multitasking: A Pilot Study

2015 ◽  
Vol 22 (3) ◽  
pp. 94-101 ◽  
Author(s):  
Li Hsieh
Keyword(s):  
Executive Control ◽  
Positive Impact ◽  
Reaction Times ◽  
Language Experience ◽  
Bilingual Advantage ◽  
Bilingual Speakers ◽  
Proficiency Level ◽  
Linguistic Representations ◽  
A Cell ◽  
The Difference

Bilingual speakers rely on attentional and executive control to continuously inhibit or activate linguistic representations of competing languages, which leads to an increased efficiency known as “bilingual advantage”. Both monolingual and bilingual speakers were asked to perform multiple tasks of talking on a cell phone while simultaneously attending to simulated driving events. This study examined the effect of bilingualism on participants' performance during a dual-task experiment based on 20 monolingual and 13 bilingual healthy adults. The within-subject and between-subject comparisons were conducted on reaction times of a visual event detection task for (a) only driving and (b) driving while simultaneously engaged in a phone conversation. Results of this study showed that bilingual speakers performed significantly faster than monolingual speakers during the multitasking condition, but not during the driving only condition. Further, bilingual speakers consistently showed a bilingual advantage in reaction times during the multitasking condition, despite varying degrees on a bilingual dominance scale. Overall, experiences in more than one language yield bilingual advantage in better performance than monolingual speakers during a multitasking condition, but not during a single task condition. Regardless of the difference in bilingual proficiency level, such language experience reveals a positive impact on bilingual speakers for multitasking.

scholarly journals A Robust Rerank Approach for Feature Selection and Its Application to Pooling-Based GWA Studies

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jia-Rou Liu ◽  
Po-Hsiu Kuo ◽  
Hung Hung
Keyword(s):  
Characteristic Curve ◽  
Real Data ◽  
Tuning Parameter ◽  
Practical Implementation ◽  
Tuning Parameters ◽  
Genome Wide ◽  
Feature Based ◽  
The Difference ◽  
Gwa Studies ◽  
Selection Of

Large-p-small-ndatasets are commonly encountered in modern biomedical studies. To detect the difference between two groups, conventional methods would fail to apply due to the instability in estimating variances int-test and a high proportion of tied values in AUC (area under the receiver operating characteristic curve) estimates. The significance analysis of microarrays (SAM) may also not be satisfactory, since its performance is sensitive to the tuning parameter, and its selection is not straightforward. In this work, we propose a robust rerank approach to overcome the above-mentioned diffculties. In particular, we obtain a rank-based statistic for each feature based on the concept of “rank-over-variable.” Techniques of “random subset” and “rerank” are then iteratively applied to rank features, and the leading features will be selected for further studies. The proposed re-rank approach is especially applicable for large-p-small-ndatasets. Moreover, it is insensitive to the selection of tuning parameters, which is an appealing property for practical implementation. Simulation studies and real data analysis of pooling-based genome wide association (GWA) studies demonstrate the usefulness of our method.

scholarly journals Do experienced physiotherapists apply equal magnitude of force during a grade I central pa on the cervical spine?

2004 ◽  
Vol 60 (4) ◽  
Author(s):  
M. Conradie ◽  
E. Smit ◽  
M. Louw ◽  
M. Prinsloo ◽  
L. Loubser ◽  
...  
Keyword(s):  
Cervical Spine ◽  
Spinal Disorders ◽  
Joint Mobilization ◽  
Treatment Techniques ◽  
Average Maximum ◽  
Manual Examination ◽  
The Mean ◽  
The Difference ◽  
Posterior Anterior ◽  
Selection Of

Background and purpose: Physiotherapists frequently use central posterior-anterior (PA) joint mobilization techniques for assessing and managing spinal disorders. Manual examination findings provide the basis for the selection of treatment techniques. From the literature it is evident that the level of reliability varies when physiotherapists perform different mobilization techniques. Repeatability of mobilization techniques is important for better physiotherapy management. The aim of the study was to determine whether experienced physiotherapists apply equal magnitude of force during a grade I central PA mobilisation technique on the cervical spine. Another aim was to determine the variation in the magnitude of forceapplied by each individual physiotherapist. Subjects: Sample of convenience, consisting of sixteen (n=16) selected qualified physiotherapists with experience inOrthopaedic Manual Therapy.Methods: A grade I central PA was performed on the Flexiforce TM sensors positioned on C6 of the same asymptomatic model to measure the applied magnitude of force. Two separate measurements, each lasting 30-seconds, were obtained.Results: The average maximum peak force applied by the majority of physiotherapists (87.5%) was between 10.95gand 72g. The difference in the forces applied for the two measurements ranged between 0.64g and 24.4g. The BlandAltman scatterplot determined the mean of the difference between measurement one and two, calculated for the group, was zero. When comparing the two measurements, little variation was noted in the forces applied, as well as the coefficient of variation for each physiotherapist.Conclusion and Discussion: Current results demonstrated good intra-therapist and moderate to good inter-therapist repeatability. Further research is required to generalize results.

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