Evidence for cross-talk between stanniocalcins

2005 ◽  
Vol 83 (11) ◽  
pp. 953-956 ◽  
Author(s):  
Mark Paciga ◽  
Kathi James ◽  
J Ryan J Gillespie ◽  
Graham F Wagner
Keyword(s):  
Molecular Weight ◽  
Receptor Binding ◽  
Cross Talk ◽  
The Other ◽  
Luteal Cell ◽  
Binding Assays ◽  
Inhibitory Effects ◽  
Intracellular Targeting ◽  
Liver And Kidney ◽  
Tissue Receptors

There are 2 forms of stanniocalcin (STC) produced by the STC-1 gene; a 50 kDa polypeptide known as STC50 and a recently discovered group of higher molecular weight variants that are collectively referred to as big STC. Both have different tissue patterns of expression and different intracellular targeting pathways. STC50 functions locally in tissues such as muscle, liver, and kidney and is targeted to mitochondria. Big STC, on the other hand, is made by the ovaries. It signals both locally on nearby corpus luteal cells and systemically. Interestingly, however, receptor binding assays employing STC50 as the tracer have shown that the smaller ligand can bind equally to tissue receptors targeted by either form of the hormone. This suggests there may be cross-talk between ligands. The present study provides credence to this notion by demonstrating how the 2 hormones can compete for tissue receptors normally targeted by 1 form of the hormone (big STC). The results also reveal how STC50 can completely block the inhibitory effects of big STC on luteal cell progesterone release when added simultaneously. The findings therefore add credence to the possibility that there may be circumstances during which the 2 ligands functionally antagonize each other's actions.Key words: stanniocalcin (STC), STC50, big STC, receptor, antagonism, progesterone release.

Impact of IgG2 high molecular weight species on neonatal Fc receptor binding assays

2015 ◽  
Vol 489 ◽  
pp. 25-31 ◽  
Author(s):  
Yuling Zhang ◽  
Abhishek Mathur ◽  
Gwen Maher ◽  
Thomas Arroll ◽  
Robert Bailey
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Receptor Binding ◽  
Fc Receptor ◽  
Binding Assays ◽  
Neonatal Fc Receptor ◽  
High Molecular Weight Species

The Cyclic-Amp-Lowering Effect Of Pge2 And Of The PGH2 Analog, U46619

1981 ◽  
Author(s):  
B Martin ◽  
C Bonne
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Mechanism Of Action ◽  
Human Platelets ◽  
The Other ◽  
Adenylate Cyclase System ◽  
Binding Assays ◽  
Inhibitory Effects ◽  
Lowering Effect ◽  
Specific Antagonist

We have recently reported that PGE2 counteracted the anti-aggregating action of PGE1 and of other PG by inhibiting the adenylate cyclase system. But this effect is not due to the interference with anti-aggregating PG receptors. In this study, the mechanism of action of the c AMPlowering effect of PGE2 has been further investigated.Suspensions of aspirin-washed human platelets were incubated for 1 min at 37°C in the presence of papaverine (0.5mM) with PGE1 , PGE2, U46619, a chemical analog of PGH2, 13-azaprostanoic acid, a specific antagonist of TxA2, either alone or in various associations. The c AMP concentration was determined by protein binding assays in platelet extracts. PGE2 (l50nM) and U46619 (1μM) inhibited the rise in c AMP induced by PGE1 (30nM) On the other hand, when 13-azaprostanoic acid (507#x03BC;M) was added to the incubate, the inhibitory effects of these compounds were suppressed.These results support the conclusion that TxA2 and U46619 act on a unique receptor which triggers the c AMP-lowering effect and suggest that PGE2 antagonizes the anti-aggregating PG through interaction with this receptor.

The Kidney and Fibrinolysis

1970 ◽  
Vol 24 (01/02) ◽  
pp. 026-032 ◽  
Author(s):  
N. A Marsh
Keyword(s):  
Molecular Weight ◽  
Plasminogen Activator ◽  
Enzyme System ◽  
Normal Animal ◽  
Fibrinolytic Enzyme ◽  
The Other ◽  
Exclusion Chromatography ◽  
Normal Urine ◽  
Renal Origin ◽  
Molecular Exclusion Chromatography

SummaryMolecular exclusion chromatography was performed on samples of urine from normal and aminonucleoside nephrotic rats. Normal urine contained 2 peaks of urokinase activity, one having a molecular weight of 22,000 and the other around 200,000. Nephrotic urine contained three peaks of activity with MW’s 126,000, 60,000 and 30,000. Plasma activator determined from euglobulin precipitate had a MW. in excess of 200,000. The results indicate that in the normal animal, plasma plasminogen activator does not escape into the urine in substantial quantities but under the conditions of extreme proteinuria there may be some loss through the kidney. The alteration in urokinase output in nephrotic animals indicates a greatly disordered renal fibrinolytic enzyme system.The findings of this study largely support the hypothesis that plasma plasminogen activator of renal origin and urinary plasminogen activator (urokinase) are different molecular species.

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

Barricyclin D1—a dimeric ellagitannin with a macrocyclic structure—and accompanying tannins from Barringtonia racemosa

2021 ◽  
Author(s):  
Shinji Yoshikawa ◽  
Lih-Geeng Chen ◽  
Morio Yoshimura ◽  
Yoshiaki Amakura ◽  
Tsutomu Hatano ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Natural Resource ◽  
The Other ◽  
Hydrolysable Tannin ◽  
The Family ◽  
Macrocyclic Structure

Abstract Our examination of high molecular weight polyphenolic constituents in the leaves of Barringtonia racemosa of the family Lecythidaceae uncovered five previously undescribed ellagitannins. One, barringtin M1 (1), among them was a hydrolysable tannin monomer, while remaining four, barringtins D1 (2), D2 (3), D3 (4) and barricyclin D1 (5), were all dimers. Barricyclin D1 had a first macrocyclic structure formed from casuarictin (6) and tellimagrandin I (7), and the other ellagitannins had structures related to 5. Two additional known phenolics, valoneic acid dilactone (8) and schimawalin A (9), were also isolated from the leaves. These results suggested that the leaves of B. racemosa is a natural resource rich in hydrolysable tannin oligomers.

scholarly journals Responses of the Pheromone-Binding Protein of the Silk Moth Bombyx mori on a Graphene Biosensor Match Binding Constants in Solution

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 499
Author(s):  
Caroline Bonazza ◽  
Jiao Zhu ◽  
Roger Hasler ◽  
Rosa Mastrogiacomo ◽  
Paolo Pelosi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Binding Protein ◽  
Binding Constants ◽  
Isoamyl Acetate ◽  
The Other ◽  
Oxide Surface ◽  
Binding Assays ◽  
Pheromone Binding Protein ◽  
Silk Moth ◽  
Good Agreement

An electronic biosensor for odors was assembled by immobilizing the silk moth Bombyx mori pheromone binding protein (BmorPBP1) on a reduced graphene oxide surface of a field-effect transistor. At physiological pH, the sensor detects the B. mori pheromones, bombykol and bombykal, with good affinity and specificity. Among the other odorants tested, only eugenol elicited a strong signal, while terpenoids and other odorants (linalool, geraniol, isoamyl acetate, and 2-isobutyl-3-methoxypyrazine) produced only very weak responses. Parallel binding assays were performed with the same protein and the same ligands, using the common fluorescence approach adopted for similar proteins. The results are in good agreement with the sensor’s responses: bombykol and bombykal, together with eugenol, proved to be strong ligands, while the other compounds showed only poor affinity. When tested at pH 4, the protein failed to bind bombykol both in solution and when immobilized on the sensor. This result further indicates that the BmorPBP1 retains its full activity when immobilized on a surface, including the conformational change observed in acidic conditions. The good agreement between fluorescence assays and sensor responses suggests that ligand-binding assays in solution can be used to screen mutants of a binding protein when selecting the best form to be immobilized on a biosensor.

Characterisation of Persistent Anti-Xa Activity Following Administration of the Low Molecular Weight Heparin Enoxaparin Sodium (Clexane)

1994 ◽  
Vol 72 (02) ◽  
pp. 275-280 ◽  
Author(s):  
David Brieger ◽  
Joan Dawes
Keyword(s):  
Molecular Weight ◽  
Enoxaparin Sodium ◽  
Antithrombin Iii ◽  
Anticoagulant Activity ◽  
Active Material ◽  
Biologically Active ◽  
Size Exclusion ◽  
Low Molecular Weight ◽  
Exclusion Chromatography ◽  
Liver And Kidney

SummaryIt is widely reported that persistent anti-Xa activity follows administration of low molecular weight heparins. To identify the effectors of this activity we have injected 125I-labelled Enoxaparin sodium into rabbits and subsequently analysed the circulating radiolabelled material and anti-Xa activity by affinity and size exclusion chromatography. Antithrombin III-binding material derived from the injected drug was responsible for all the anti-Xa amidolytic activity. At early times after injection additional anticoagulant activity which was largely attributable to tissue factor pathway inhibitor was measured by the Heptest clotting assay after removal of glycosaminoglycans from plasma samples. Small radiolabelled fragments, including penta/hexasaccharide with affinity for antithrombin III, were detectable in the circulation 1 week later, and sulphated oligosaccharides persisted for 3-4 weeks. Significant quantities of radiolabel remained in the liver and kidney several weeks post-injection; these organs may sequester some of the injected drug and give rise to circulating biologically active material by degradation and secretion of catabolic products into the plasma.

STUDIES ON THE ANTITHROMBOTIC ACTION OF A LOW MOLECULARWEIGHT HEPARIN OBTAINED FROM BEEF MUCOSAL ORIGIN AND PEROXIDATIVE CLEAVAGE

1987 ◽  
Author(s):  
P Bianchini ◽  
R Nonn ◽  
J Fareed ◽  
J M Walenga ◽  
A Kumar
Keyword(s):  
Molecular Weight ◽  
Structural Integrity ◽  
Factor Xa ◽  
Inhibitory Effects ◽  
Heparin Cofactor Ii ◽  
At Iii ◽  
Antithrombotic Effects ◽  
In Vitro Systems ◽  
Mean Molecular Weight

We have studied a low molecular weight heparin (LMWH) obtained by acontrolled peroxidative depolymerization of beef mucosal heparin (OP 2123, Opocrin, Corlo, Italy). This product was found to be significantly different from other LMWHs in that it exhibits the same COO-/SO2- ratios as unfractionated heparin, contains reducing end groups composed of 2-sulfated iduronic acid or 6-disulfated glucosamine and retains an identical structural integrity as that of native heparin. As opposed to most other LMWHs the oligosaccharide components of OP 2123 consist of homogeneous progressive units. In addition, the relative amount of AT-IIIaffinity components in OP 2123 were 20-30% less than other LMWHs. OP 2123 has a mean molecular weight of 6200 daltons with a potency of 90 anti-factor Xa U/mg and 68 USP U/mg. This agent produced strong antithrombotic actions in a rabbit stasis model against both an activated prothrombin complex and a prothrombin complex concentrate/Russell's viper venomcombination (ED50:(IV) 30-70 ug/kg;(SC) 0.6-1.5 mg/kg). The antithrombotic effects were comparable to other LMWHs in normal rabbits: however, in AT III depleted rabbits (immunodepleted and y thrombin depleted), OP 2123 produced stronger antithrombotic effects than most other LMWHs. The in vitro systems in contrast to other LMWHs, CP 2123 produced stronger inhibitory effects in AT III depleted plasma as measured by fibrinopeptide A generation and amidolytic anti-factor Xa and anti-factor Ila methods. The relative heparin cofactor II activity as measured by amidolytic method was also found to be higher than with most LMWHs. These results suggest that OP 2123, unlike most LMWHs, non AT III mediated actions play a major role in themendiation of the antithrombotic actions.

CO-ORDINATION COMPLEXES OF TITANIUM (IV) HALIDES: II. PREPARATION AND INFRARED SPECTRA OF THE COMPLEXES OF TITANIUM TETRACHLORIDE WITH DIESTERS OF α,ω-DICARBOXYLIC ACIDS AND COMPARISON WITH ANALOGOUS COMPLEXES OF ZIRCONIUM TETRACHLORIDE AND TIN TETRACHLORIDE

1961 ◽  
Vol 39 (11) ◽  
pp. 2343-2352 ◽  
Author(s):  
Ernest Rivet ◽  
Real Aubin ◽  
Roland Rivest
Keyword(s):  
Molecular Weight ◽  
Infrared Spectra ◽  
Titanium Tetrachloride ◽  
Complex Molecule ◽  
Dicarboxylic Acids ◽  
The Other ◽  
Zirconium Tetrachloride ◽  
Melting Points ◽  
Zirconium Complexes ◽  
Tin Tetrachloride

Co-ordination complexes between diesters of α,ω-dicarboxylic acids and titanium tetrachloride, tin tetrachloride, and zirconium tetrachloride have been prepared. The analytical results, the infrared spectra, the melting points, and the molecular-weight determinations indicate that for the titanium and zirconium complexes, two types of complexes are obtained, one having a general formula MX4•1 diester in which chelate rings from five to nine atoms are formed and the other one, 2MX4•1 diester in which there are two 4-membered rings per complex molecule. With tin tetrachloride only one type of complex is formed, which has two tin tetrachlorides and two diesters per complex molecule.

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