Characterization of [3H]-forskolin binding sites in young and adult rat brain cortex: identification of suramin as a competitive inhibitor of [3H]-forskolin binding

2005 ◽  
Vol 83 (7) ◽  
pp. 573-581 ◽  
Author(s):  
Jiri Stöhr ◽  
Jiri Novotny ◽  
Petr Svoboda
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Brain Cortex ◽  
Specific Binding ◽  
Age Groups ◽  
Competitive Inhibitor ◽  
Adenyl Cyclase Activity ◽  
High Affinity ◽  
Young Rats ◽  
Rat Brain Cortex

Little is know about forskolin binding in the rat brain during ontogenetic development. For this paper, we have characterized specific binding sites for [3H]-forskolin in cerebrocortical membranes from young (12-day-old) and adult (90-day-old) rats. High-affinity, as well as super-high-affinity, [3H]-forskolin binding sites were detected in samples from both age groups tested, and the binding parameters of these sites differed significantly. Whereas the number of high-affinity [3H]-forskolin binding sites was higher by about 50% in adult than in young rats, their affinity was markedly (about 4 times) lower. In the presence of AlF4–, the number high-affinity [3H]-forskolin binding sites in samples from young rats rose to the level determined in samples from adult animals, and the number of super-high-affinity sites considerably increased in both age groups. The different characteristics of [3H]-forskolin binding found in cerebrocortical membranes from young and adult rats may be closely related to markedly diminished adenyl cyclase activity in preparations from adult animals. Results of our experiments with suramin indicated that this drug may act as a competitive inhibitor of [3H]-forskolin binding. Key words: rat brain cortex; development; forskolin binding; suramin.

Identity of inhibitory presynaptic 5-hydroxytryptamine (5-HT) autoreceptors in the rat brain cortex with 5-HT1B binding sites

1986 ◽  
Vol 332 (1) ◽  
pp. 1-7 ◽  
Author(s):  
G. Engel ◽  
M. G�thert ◽  
D. Hoyer ◽  
E. Schlicker ◽  
K. Hillenbrand
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Brain Cortex ◽  
Rat Brain Cortex

Subcellular distribution of rat brain cortex high-affinity, sodium-dependent, glycine transport sites

1977 ◽  
Vol 122 (1) ◽  
pp. 95-112 ◽  
Author(s):  
Fernando Valde´s ◽  
Carlos Mun˜oz ◽  
Alfredo Feria-Velasco ◽  
Fernando Orrego
Keyword(s):  
Rat Brain ◽  
Subcellular Distribution ◽  
Brain Cortex ◽  
High Affinity ◽  
Rat Brain Cortex ◽  
Sodium Dependent ◽  
Glycine Transport

Melatonin-binding sites in the rat brain and pituitary mapped by in-vitro autoradiography

1989 ◽  
Vol 3 (1) ◽  
pp. 71-75 ◽  
Author(s):  
L. M. Williams
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Area Postrema ◽  
Specific Binding ◽  
Melatonin Receptors ◽  
Pars Tuberalis ◽  
Lower Affinity ◽  
High Affinity ◽  
In Vitro Autoradiography

ABSTRACT Using picomolar concentrations of [125I]iodomelatonin and in-vitro autoradiography, specific melatonin-binding sites have been mapped in the rat brain and pituitary. Using this same technique, high-affinity melatonin receptors had previously been identified in the suprachiasmatic nucleus (SCN) and median eminence regions of the rat hypothalamus. The presence of melatonin binding in the SCN has been confirmed, but the second area of binding has been identified as the pars tuberalis of the pituitary, and a completely novel area of binding is also reported in the area postrema. The existence of lower affinity melatonin receptors in the rat brain was also investigated using in-vitro autoradiography and higher concentrations of [125I]iodomelatonin. No further sites of specific binding were, however, disclosed.

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