The effects of ketorolac and morphine on articular cartilage and synovium in the rabbit knee joint

2004 ◽  
Vol 82 (7) ◽  
pp. 502-505 ◽  
Author(s):  
Nazim Dogan ◽  
Ali Fuat Erdem ◽  
Cemal Gundogdu ◽  
Husnu Kursad ◽  
Mehmet Kizilkaya
Keyword(s):  
Articular Cartilage ◽  
Knee Joint ◽  
Synovial Membrane ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Knee Joints ◽  
Cell Infiltration ◽  
Histopathological Changes ◽  
Rabbit Knee ◽  
Group I

Analgesics are commonly injected intra-articularly for analgesia after arthroscopic surgery, especially of knee joints. The aim of this study was to research the effects of ketorolac and morphine on articular cartilage and synovial membrane. This study used rabbit right and left hind knee joints. The treatments, saline, morphine, or ketorolac, were administered intra-articularly 24 h after injection, and 5 joints from animals in each drug group were chosen randomly to form Group I and subgroups of Group I. The same procedures were applied after 48 h and 10 days of injection to form Groups II and III, respectively, and subgroups of these groups. Knee joints were excised and a blinded observer evaluated the histopathology according to inflammation of the articular cartilage, inflammatory cell infiltration, hypertrophy, and hyperplasia of the synovial membrane. No histopathological changes were found in the control groups. In the ketorolac and morphine groups, there were varying degrees of synovial membrane inflammatory cell infiltration and minimal, mild, or moderate synovial membrane cell hyperplasia or hypertrophy. Except for the ketorolac group at 24 h, both ketorolac and morphine groups showed more histopathological changes than controls (p < 0.05). Morphine and ketorolac both cause mild histopathological changes in rabbit knee joints, morphine causing more than ketorolac, but both of the drugs can be used intra-articularly with safety.Key words: intra-articular analgesia, knee joint, histopathological changes, articular cartilage, synovial membrane.

scholarly journals The Possible Therapeutic Role of Platelet Rich Plasma on a Model of Osteoarthritis in Male Albino Rat. Histological and Immunohistochemical Study

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
N Gamal ◽  
N M Abourabia ◽  
F H Elebiary ◽  
G Khalaf ◽  
M H Raafat
Keyword(s):  
Articular Cartilage ◽  
Knee Joint ◽  
Rat Model ◽  
Synovial Membrane ◽  
Platelet Rich Plasma ◽  
Knee Joints ◽  
Group I ◽  
Group 15 ◽  
Group Ii ◽  
The Right

Abstract Introduction and Aim of the Study Osteoarthritis (OA) is a degenerative joint disease characterized by joint pain and progressive loss of articular cartilage. This study was conducted to evaluate the therapeutic effect of platelet-rich plasma (PRP) of the knee joint in a rat model of OA. Materials and Methods Forty adult male albino rats, weighing 200-250 gms, were used in this study, ten rats used as donors to obtain PRP. The other thirty rats were divided into two main groups. Group I: The control group (15 rats) in which the rats were subdivided into three subgroups. Subgroups IA and IB were sacrificed 4 and 6 weeks after the beginning of the experiment. Subgroup IC left for 4 weeks then received intra-articular injection of PRP in the right knee joint which was repeated three times per week for 2 weeks then the animals were sacrificed. Group II (The experimental group) (15 rats) in which OA was induced by surgical induction of cartilaginous defect in the right knee joints. The rats of group II were subdivided into three subgroups. Subgroups IIA and IIB were sacrificed 4 and 6 weeks after induction of OA respectively. Subgroup IIC received intra-articular injection of PRP (0.2 ml) in the right knee joints 4 weeks after surgery. The injection was repeated three times per week for 2 weeks then the animals were sacrificed. The right joints from all groups were collected, decalcified and processed for histological studies. Specimens were also processed for transmission electron microscopic study. Morphometric and statistical measurements were done. Results Histological examination of the right knee joints of OA (subgroups IIA and IIB) resulted in thickening of the intimal lining of the synovial membrane, cellular infiltration and increased collagen content of the subintima. The articular cartilage showed erosions, thinning of cartilage, chondrocytes and ground substance loss, and moderate expression of platelet derived growth factor (PDGF). Injection of PRP resulted in improvement of the structure of the synovial membrane and the articular cartilage and strong expression of PDGF. Conclusion Intra-articular injection of PRP resulted in a significant improvement in the histological structure of the knee joint in a rat model of OA.

scholarly journals Attenuation of lipopolysaccharide-induced lung inflammation by ascorbic acid in rats: Histopathological and ultrastructural study

2019 ◽  
Vol 7 ◽  
pp. 205031211982826 ◽  
Author(s):  
Hazem Abdelhamid Mohamed ◽  
Yasser M Elbastawisy ◽  
Wael M Elsaed
Keyword(s):  
Ascorbic Acid ◽  
Basement Membrane ◽  
Lung Injury ◽  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Group Iii ◽  
Ultrastructural Studies ◽  
Group I ◽  
Group Ii

Introduction: Lipopolysaccharide is a bacterial endotoxin that induces acute lung injury in experimental animals, which is similar to acute respiratory distress syndrome in humans. The induced tissue trauma ends in fibrosis. Understanding the pathogenesis is important in the prevention and treatment of the complications. This study was assigned to investigate the long-term lipopolysaccharide-induced lung injury and the postulated protective effect of ascorbic acid on these changes. Materials and methods: Twenty-four adult male albino rats were divided into three groups. Group I was the controls, group II received lipopolysaccharide and group III received lipopolysaccharide and ascorbic acid. After 30 days of starting treatment, lung tissue samples were obtained. Results: Group II lung tissues showed marked thickening of the alveolar septa with collapsed alveolar sacs, detached bronchial epithelium, inflammatory cell infiltration and excessive deposition of collagen. Group III showed mild thickening of the alveolar walls, scanty inflammatory cell infiltration, mild parabronchial fibrosis and less marked collagen deposition. α-Smooth muscle actin staining of group II showed marked expression of the actin-positive cells. Less potential expression of the dye was found in group III. Ultrastructural examination of group II showed evident structural changes in pneumocytes with capillary basement membrane irregularity and interruption compared to uniform basement membrane in group III with less prominent intracellular changes in pneumocytes. Conclusion: Ascorbic acid attenuated the inflammatory response and fibrosis in the lungs of rats treated with lipopolysaccharide as evidenced by the histological, immunohistochemical and ultrastructural studies.

EFFECT OF POVIDONE-IODINE ON THE SYNOVIAL MEMBRANE OF THE KNEE JOINT IN RATS

2000 ◽  
Vol 04 (04) ◽  
pp. 249-255
Author(s):  
Jeong-Lim Moon ◽  
Jang-Cheol Sihn ◽  
Myung-Sang Moon
Keyword(s):  
Knee Joint ◽  
Synovial Membrane ◽  
Povidone Iodine ◽  
Knee Joints ◽  
Group I ◽  
Group Ii

The effect of two different concentrations of povidone-iodine (PVI) solution, an antiseptic, on joint synovium was investigated. In Group I, 0.05 ml of 10% PVI was used, while in Group II, 0.05 ml of 2.5% of PVI was used. PVI solution was injected twice into both knee joints with one week interval. Ten rats were used as control and 70 rats as experimental. In the two experimental groups four rats were sacrificed after 6, 12, 24 hours and three day and six rats at the end of the week after the second PVI injection. Synovial reaction was assessed histologically in both groups, based on the pathological parameters. The results suggest that intra-articular injection of 10% and 2.5% PVI induces synovitis with focal ulceration which gradually subsides, and finally, the synovium becomes normal, though various degrees of subsynovial dense fibrosis complication arise.

The effect of OK-432 (Picibanil) injection on the histopathology of nasal turbinate

2015 ◽  
Vol 129 (12) ◽  
pp. 1208-1212
Author(s):  
S Sengul ◽  
İ Kaygusuz ◽  
M M Akin ◽  
Ş Yalcin ◽  
T Karlidag ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Cell Loss ◽  
Treated Group ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Saline Control ◽  
Histopathological Changes ◽  
Nasal Turbinate ◽  
Treatment Groups ◽  
Significant Difference

AbstractObjective:This study aimed to assess the histopathological effect of OK-432 (Picibanil) on rabbit nasal turbinates.Methods:A total of 21 rabbits were divided into 3 treatment groups and various parts of both nasal turbinates were injected with 0.5 ml OK-432, 0.2 ml OK-432 or 0.6 ml saline (control). Bilateral nasal turbinates were later excised and studied under light microscopy to assess any histopathological changes.Results:Animals in the 0.2 ml and 0.5 ml OK-432 groups exhibited mild ciliary loss, goblet cell loss and epithelial damage, and a marked increase in inflammatory cell infiltration, submucosal vascularisation and fibrosis. There was a significant difference in histopathological changes between the two OK-432 treated groups. In addition, each OK-432 treated group had significantly more inflammatory cell infiltration, increased submucosal vascularisation and fibrosis compared with controls.Conclusion:The marked fibrosis observed in OK-432-injected turbinates may be responsible for a reduction in turbinate size.

Effects of Osmium Tetroxide on the Rabbit Knee Joint Normal Synovial Membrane

1987 ◽  
Vol 16 (1) ◽  
pp. 121-129
Author(s):  
M. Möttönen ◽  
M. Pantio ◽  
T. Nevalainen
Keyword(s):  
Knee Joint ◽  
Synovial Membrane ◽  
Osmium Tetroxide ◽  
Rabbit Knee

A Case of Bullous Amyloidosis Associated with an Inflammatory Cell Infiltration and Prominent Pruritus

2008 ◽  
Vol 70 (3) ◽  
pp. 269-273
Author(s):  
Taisuke KAMIYAMA ◽  
Yoshihiro KAWAGUCHI ◽  
Masami SASAKI ◽  
Masamichi SATOU ◽  
Kumiko MIURA ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration

scholarly journals The Symmetric 3D Organization of Connective Tissue around Implant Abutment: A Key-Issue to Prevent Bone Resorption

Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1126
Author(s):  
Giovanna Iezzi ◽  
Francesca Di Lillo ◽  
Michele Furlani ◽  
Marco Degidi ◽  
Adriano Piattelli ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Connective Tissue ◽  
Inflammatory Cell ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Collagen Fibers ◽  
Inflammatory Cell Infiltration ◽  
Cell Infiltration ◽  
Oral Epithelium ◽  
Implant Abutment

Symmetric and well-organized connective tissues around the longitudinal implant axis were hypothesized to decrease early bone resorption by reducing inflammatory cell infiltration. Previous studies that referred to the connective tissue around implant and abutments were based on two-dimensional investigations; however, only advanced three-dimensional characterizations could evidence the organization of connective tissue microarchitecture in the attempt of finding new strategies to reduce inflammatory cell infiltration. We retrieved three implants with a cone morse implant–abutment connection from patients; they were investigated by high-resolution X-ray phase-contrast microtomography, cross-linking the obtained information with histologic results. We observed transverse and longitudinal orientated collagen bundles intertwining with each other. In the longitudinal planes, it was observed that the closer the fiber bundles were to the implant, the more symmetric and regular their course was. The transverse bundles of collagen fibers were observed as semicircular, intersecting in the lamina propria of the mucosa and ending in the oral epithelium. No collagen fibers were found radial to the implant surface. This intertwining three-dimensional pattern seems to favor the stabilization of the soft tissues around the implants, preventing inflammatory cell apical migration and, consequently, preventing bone resorption and implant failure. This fact, according to the authors’ best knowledge, has never been reported in the literature and might be due to the physical forces acting on fibroblasts and on the collagen produced by the fibroblasts themselves, in areas close to the implant and to the symmetric geometry of the implant itself.

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