Fourier-transform infrared spectroscopy: a pharmacotoxicologic tool for in vivo monitoring radical aggression

2001 ◽  
Vol 79 (2) ◽  
pp. 158-165 ◽  
Author(s):  
Anne-Marie Melin ◽  
Annie Perromat ◽  
Gérard Deleris
Keyword(s):  
Fourier Transform ◽  
Carbon Tetrachloride ◽  
Ir Spectroscopy ◽  
Liver Microsomes ◽  
Fourier Transform Infrared ◽  
Conformational Structure ◽  
Ft Ir ◽  
The Brain ◽  
Ft Ir Spectroscopy

Among the physico-chemical methods that can be used to investigate induced peroxidation in living cells, Fourier transform infrared (FT-IR) spectroscopy appears to be a valuable technique as it is non-destructive and sensitive for monitoring changes in the vibrational spectra of samples. We examined microsomal fractions from rat liver and brain by FT-IR to study the effect of radical aggression induced in vivo by carbon tetrachloride (CCl4). The length of the acyl chains was increased as a consequence of peroxidation induced by the xenobiotic. Moreover, an enhanced level of cholesterol esters and an increase in phospholipids were observed in the liver and the brain, respectively. The conformational structure of the membrane proteins was changed in both the liver and the brain. In the polysaccharide region, we observed an important loss in glucidic structures, such as a decrease in liver glycogen and in some brain glycolipids. These alterations are probably due to the interactions between cells and CCl4and the metabolic changes caused by CCl4. Thus, FT-IR spectroscopy appears to be an useful tool and an accurate means for rapidly investigating the in vivo biochemical alterations induced by CCl4in microsomes, and for correlating them with biochemical and physiological data.Key words: brain, carbon tetrachloride, FT-IR, liver, microsomes.

The in vivo toxicity of carbon tetrachloride and carrageenan on heart microsomes: analysis by Fourier transform infrared spectroscopy

2001 ◽  
Vol 79 (9) ◽  
pp. 799-804 ◽  
Author(s):  
Anne-Marie Melin ◽  
Annie Perromat ◽  
Gérard Deleris
Keyword(s):  
Fourier Transform ◽  
Carbon Tetrachloride ◽  
Ir Spectroscopy ◽  
Rat Heart ◽  
Great Part ◽  
Total Lipid Content ◽  
Fourier Transform Infrared ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

We investigated the sensitivity of rat heart microsomes to free radical attack using Fourier transform infrared (FT-IR) spectroscopy. This physico-chemical method seemed a valuable technique: quite sensitive to changes in the vibrational spectra. The spectral variations observed between normal and treated rats were in great part due to reactive oxygen species that led to changes in protein conformation involving β-sheets, aggregation of proteins, and modification of protein synthesis. Carrageenan-induced inflammation slightly enhanced the total lipid content; rearrangement of acyl chains and accumulation of cholesterol esters and phospholipids also occurred in the treated rats. Carbon tetrachloride induced a decrease in both lipid and protein contents. The level of glucidic substrates was diminished with carbon tetrachloride and enhanced with carrageenan; these changes were due to metabolic interactions between cell components and drugs. FT-IR spectroscopy provided an accurate means to monitor, in rat heart, the in vivo effects of inflammatory and peroxidative damages, to discriminate and classify the affected cells, and to correlate the findings with known physiological and biochemical data in close relationship with metabolic disruptions induced by the two xenobiotics. Key words: heart microsomes, carbon tetrachloride, carrageenan, peroxidation, FT-IR.

scholarly journals Bacterial Typing and Identification Based on Fourier Transform Infrared Spectroscopy

2020 ◽  
Author(s):  
Huayan Yang ◽  
Fangling Wu ◽  
Fuxin Xu ◽  
Keqi Tang ◽  
Chuanfan Ding ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Ir Spectroscopy ◽  
Bacterial Culture ◽  
Clinical Laboratory ◽  
Fourier Transform Infrared ◽  
Identification Accuracy ◽  
Bacterial Typing ◽  
Typical Experiment ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Abstract Fourier transform infrared (FT-IR) spectroscopy is a label-free and highly sensitive technique that provides complete information on the chemical composition of biological samples. The bacterial FT-IR signals are extremely specific and highly reproducible fingerprint-like patterns, making FT-IR an efficient tool for bacterial typing and identification. Due to the low cost and high flux, FT-IR has been widely used in hospital hygiene management for infection control, epidemiological studies, and routine bacterial determination of clinical laboratory values. However, the typing and identification accuracy could be affected by many factors, and the bacterial FT-IR data from different laboratories are usually not comparable. A standard protocol is required to improve the accuracy of FT-IR-based typing and identification. Here, we detail the principles and procedures of bacterial typing and identification based on FT-IR spectroscopy, including bacterial culture, sample preparation, instrument operation, spectra collection, spectra preprocessing, and mathematical data analysis. Without bacterial culture, a typical experiment generally takes <2 h.

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