Somatic embryogenesis in longleaf pine (Pinuspalustris)

1993 ◽  
Vol 23 (5) ◽  
pp. 873-876 ◽  
Author(s):  
R. Nagmani ◽  
A.M. Diner ◽  
G.C. Sharma
Keyword(s):  
Zygotic Embryo ◽  
Carbon Sources ◽  
Embryogenic Tissue ◽  
Zygotic Embryos ◽  
Embryogenic Cultures ◽  
Embryogenic Tissue Initiation ◽  
The Media ◽  
Basal Media ◽  
Four Levels ◽  
Dichlorophenoxy Acetic Acid

Isolated zygotic embryos and female gametophytes containing zygotic embryos were cultured on MSG and DCR basal media, supplemented with three different carbon sources added individually to the medium at four levels each. The media also contained various levels of 2,4-dichlorophenoxy acetic acid (2,4-D) and N6-benzyladenine (BA). Embryogenic tissue extruded from female gametophytes during 4 weeks in culture on media containing either glucose or maltose or sucrose. Embryogenic tissue initiation was most frequently from explants collected on July 14, 1992, when the zygotic embryos within the female gametophytes were precotyledonary. A total of 33 embryogenic cultures were initiated from 944 explants cultured. One of 192 explants cultured on basal media with no growth regulators produced embryogenic tissue. The embryogenic tissue showed numerous somatic embryos at stages 1 and 2 of development, corresponding to their zygotic embryo counterparts.

Initiation of embryogenic cultures and somatic embryo development in loblolly pine (Pinustaeda)

1990 ◽  
Vol 20 (6) ◽  
pp. 810-817 ◽  
Author(s):  
M. R. Becwar ◽  
R. Nagmani ◽  
S. R. Wann
Keyword(s):  
Somatic Embryo ◽  
Embryo Development ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Basal Medium ◽  
Embryogenic Tissue ◽  
Zygotic Embryos ◽  
Embryogenic Cultures ◽  
Somatic Embryo Development

Immature zygotic embryo explants (isolated or with intact megagametophytes) from 10 loblolly pine (Pinustaeda L.) clones (7-34, 7-56, 11-9, 11-16, 11-25, 10-1003, 10-1007, 10-1011, 10-1018, and 10-1019) were surveyed for their potential to form embryogenic tissue from the suspensor region of zygotic embryos. After over 14 000 explants were cultured, embryogenic cultures were initiated from explants of 8 of the 10 clones; only explants from clones 11-25 and 10-1019 were not responsive. Embryogenic tissue was initiated from zygotic embryos with intact megagametophytes on MSG basal medium with no exogenous plant growth regulators or with 2–5 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0–1 mg/L N6-benzyladenine (BA). The highest initiation frequency (5%) was obtained from isolated zygotic embryos of clone 7-34 less than 0.5 mm in length just prior to cotyledon primordia development on DCR basal medium with 3 mg/L 2,4-D and 0.5 mg/L BA. Two types of embryogenic cultures were maintained on medium with 2,4-D and BA: (i) those that contained pre-embryonal masses of cells interspersed with unaggregated suspensorlike cells, but which rarely contained well-formed somatic embryos, and (ii) those that frequently contained well-formed somatic embryos. Somatic embryo development from both types of cultures progressed to a precotyledonary stage on medium with 2.6 mg/L abscisic acid.

scholarly journals Plant regeneration of southern Adriatic iris by somatic embryogenesis

2009 ◽  
Vol 61 (3) ◽  
pp. 413-418 ◽  
Author(s):  
Sladjana Jevremovic ◽  
Angelina Subotic ◽  
Milana Trifunovic ◽  
Marija Nikolic
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Balkan Peninsula ◽  
Embryogenic Calli ◽  
Subsequent Decrease ◽  
Simple Protocol ◽  
The Media ◽  
Dichlorophenoxy Acetic Acid

A simple protocol has been developed for plant regeneration by somatic embryogenesis of Southern Adriatic iris (Iris pseudopallida Trinajstic), an endemic species of the Balkan Peninsula. Somatic embryogenesis was induced in zygotic embryo culture on media supplemented with 2,4-dichlorophenoxy acetic acid (2-10 mgL-1) as the sole plant growth regulator, where both embryogenic calli and somatic embryos were induced. Subsequent decrease of 2,4-D in the media promoted formation of somatic embryos. Developed somatic embryos germinated on medium without growth regulators. The regenerated plantlets had diploid chromosome number. Planted plantlets acclimatized very well under greenhouse and garden conditions.

Diploid and haploid embryogenesis in Larixleptolepis, L. decidua, and their reciprocal hybrids

1990 ◽  
Vol 20 (1) ◽  
pp. 9-14 ◽  
Author(s):  
P. von Aderkas ◽  
K. Klimaszewska ◽  
J. M. Bonga
Keyword(s):  
Growth Regulators ◽  
Zygotic Embryo ◽  
Embryogenic Tissue ◽  
Zygotic Embryos ◽  
Immature Zygotic Embryos ◽  
Binucleate Cells ◽  
Meristematic Cells ◽  
Reciprocal Hybrids ◽  
Morphological Differences ◽  
Haploid Embryogenesis

Diploid and haploid embryogenesis was induced in two Larix species (L. decidua and L. leptolepis) and their reciprocal hybrids. Diploid embryogenic tissue was initiated in immature zygotic embryos isolated with the micropylar half of the megagametophyte left attached. These were placed either on modified LM or MSG medium supplemented with the growth regulators 2,4-D and 6-benzyladenine. MSG medium was solidified with either gellan gum or agar. There was no appreciable difference in response between the two. Haploid embryogenesis was induced in isolated megagametophytes placed on modified LM medium supplemented with 2,4-D and 6-benzyladenine. Diploid embryogenic tissue was subcultured on medium with growth regulators, but haploid embryogenic tissue grew well on medium without growth regulators. There were few morphological differences between the diploid and haploid embryogenic tissue. In all species and hybrids, haploid cultures contained more coenocytic long cells. Binucleate cells were most common, but tetranucleate and octanucleate cells were also present. Haploid cultures showed poorer organization than the diploid ones, with only a few cultures having well-developed embryoids. Haploid tissue originated from expanded cells of the megagametophyte. Diploid tissue originated from the suspensor region of the zygotic embryo; it proliferated from isolated clusters of meristematic cells in early embryoids. Diploid and haploid cultures differed not only from the outset, but also in the mature embryoids they produced.

Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation

2009 ◽  
Vol 39 (8) ◽  
pp. 1566-1574 ◽  
Author(s):  
Cathy L. Hargreaves ◽  
Cathie B. Reeves ◽  
Jens I. Find ◽  
Keiko Gough ◽  
Puthiyaparambil Josekutty ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Pinus Radiata ◽  
Zygotic Embryo ◽  
Embryogenic Tissue ◽  
Zygotic Embryos ◽  
Embryogenic Tissues ◽  
Preparation Techniques ◽  
Embryo Maturity ◽  
Collection Time ◽  
Excised Embryos

The principal aim of this investigation was to improve somatic embryogenesis initiation and to enhance representation of families and genotypes within those families of Pinus radiata D. Don. A total of 19 open-pollinated seed families, many with unrelated and weakly related parents, were tested. Optimum stage of cone maturity for initiation success was tested by five collections made at 1 week intervals, spanning the developmental period from pro-embryo to cotyledonary embryos. Two media were compared; embryo-development media (EDM6) and a modified Litvay medium (Glitz). Two zygotic embryo explant-preparation techniques were tested; embryos with retained megagametophytes and excised embryos. Proliferating embryogenic tissues were obtained from all four treatments (2850 explants per treatment, 570 per collection time) for the 19 families. The best initiation rates were achieved with a combination of Glitz medium with excised zygotic embryos, with 55% of explants from all collections and all families combined giving rise to proliferating embryogenic tissue. At the optimal collection time for each of the families, this treatment gave a range of 47%–97% initiation success with an average of 70% per family.

Somatic embryogenesis in mature zygotic embryos of Picea likiangensis

Biologia ◽  
2010 ◽  
Vol 65 (5) ◽  
Author(s):  
Shaoyu Chen ◽  
Shanna Chen ◽  
Fang Chen ◽  
Tao Wu ◽  
Yinbin Wang ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Clonal Propagation ◽  
Zygotic Embryos ◽  
Dichlorophenoxyacetic Acid ◽  
Embryogenic Cultures ◽  
Peg 4000 ◽  
Half Strength ◽  
Basal Media ◽  
2,4 Dichlorophenoxyacetic Acid

AbstractSomatic embryogenesis (SE) was successfully induced from mature zygotic embryos of seven families of Picea likiangensis (Franch.) Pritz after 20 weeks culture on initiation medium. Three basal media (one-half strength LM medium, one-half strength LP medium and improved LP medium) with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (6-BA) were tested but only one-half strength LM medium supplemented with 2,4-D and 6-BA was successful for the embryogenic cultures (EC) initiation. The initiation frequencies of EC varied greatly from different families when culturing on the same initiation medium. The highest frequency (41.3%) was induced from one of the families on one-half strength LM medium supplemented with 3 mg L−1 2,4-D and 1.5 mg L−1 6-BA and 16.83% on average for seven families. EC were subcultured and proliferated on the same medium as the initiation one every 10 days. 3 lines of EC induced from the same family were applied in maturation experiment. Cotyledonary somatic embryos were observed after EC were transferred to maturation media of one-half strength LM medium containing 20-80 mg L−1 abscisic acid and 7.5% polyethylene glycol (PEG-4000). However, one-half strength LM medium supplemented with 40 mg L−1 or 60 mg L−1 ABA and 7.5% PEG gave the best maturation and the 3 lines showed different ability in maturation. Over 80% cotyledonary somatic embryos germinated normally on DCR medium containing 0.2% activated carbon. The success on SE induction of the species has provided an effective clonal propagation method for this important tree’s genetic improvement.

scholarly journals Induction of somatic embryogenesis in Pinus heldreichii culture

2007 ◽  
Vol 59 (3) ◽  
pp. 199-202 ◽  
Author(s):  
Dragana Stojicic ◽  
Branka Uzelac ◽  
Dusica Janosevic ◽  
Ljubinka Culafic ◽  
Snezana Budimir
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Embryogenic Tissue ◽  
Zygotic Embryos ◽  
Dichlorophenoxyacetic Acid ◽  
Immature Zygotic Embryos ◽  
Induction Frequency ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Further Development

The potential for somatic embryogenesis in zygotic embryo and megagametophyte cultures of Pinus heldreichii was examined. Somatic embryogenesis was initiated from megagametophytes containing immature zygotic embryos at early stages of development. An induction frequency of up to 6.7% was obtained on Gresshoff and Doy medium in the presence of 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/l benzyladenine (BA). Formation and further proliferation of embryogenic tissue were achieved upon transfer of explants to a medium with reduced levels of growth regulators. Somatic embryos are being cultured for further development. .

scholarly journals In Vitro Flowering of Plantlets Regenerated from Zygotic Embryo-derived Somatic Embryos of Ginseng

HortScience ◽  
1990 ◽  
Vol 25 (12) ◽  
pp. 1652-1654 ◽  
Author(s):  
Haeng S. Lee ◽  
Jang R. Liu ◽  
Seung G. Yang ◽  
Young H. Lee ◽  
Kwang-W. Lee
Keyword(s):  
Somatic Embryos ◽  
Zygotic Embryo ◽  
Ginseng Root ◽  
Zygotic Embryos ◽  
Ms Medium ◽  
Root Explants ◽  
Half Strength ◽  
Dichlorophenoxy Acetic Acid ◽  
Somatic Embryo Induction

Mature zygotic embryos dissected from ginseng (Panax ginseng C.A. Meyer) seeds were cultured on Murashige and Skoog (MS) medium containing various concentrations of 2,4-D and kinetin. Somatic embryos were induced directly from cotyledonary tissue and from intervening callus. The frequency of somatic embryo induction was up to 55% of zygotic embryo explants. Upon transfer onto half-strength MS medium supplemented with 1 mg BA/liter and 1 mg GA3/liter, most somatic embryos developed into plantlets. More than 50% of the plantlets flowered after 4 weeks of culture, and some developed immature fruits in vitro. These results indicate that adulthood of ginseng root explants is not a prerequisite for flowering of plantlets regenerated through somatic embryogenesis. Chemical names used: (2,4 -dichlorophenoxy) acetic acid (2,4-D); N-(2-furanylmethyl) -1H-purin-6-amine(kinetin); N-(phenylmethyl) -1H-purin-6-amine (BA); gibberellic acid (GA3).

scholarly journals Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse

2021 ◽  
Vol 9 (3) ◽  
pp. 533
Author(s):  
Alex Graça Contato ◽  
Tássio Brito de Oliveira ◽  
Guilherme Mauro Aranha ◽  
Emanuelle Neiverth de Freitas ◽  
Ana Claudia Vici ◽  
...  
Keyword(s):  
Carbon Sources ◽  
Temporal Analysis ◽  
Industrial Applications ◽  
Lignocellulolytic Enzymes ◽  
Tamarindus Indica ◽  
Acetyl Xylan Esterase ◽  
Trametes Hirsuta ◽  
Hymenaea Courbaril ◽  
The Media ◽  
Main Components

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.

scholarly journals Plant regeneration and transformation of Trachyspermum ammi using Agrobacterium tumefaciens and zygotic embryos

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Masoumeh Nomani ◽  
Masoud Tohidfar
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Regeneration ◽  
Genetic Transformation ◽  
Zygotic Embryo ◽  
Plant Biotechnology ◽  
Hplc Method ◽  
Zygotic Embryos ◽  
Naphthaleneacetic Acid ◽  
Transformed Plants ◽  
Trachyspermum Ammi

Abstract Background Trachyspermum ammi is one of the key medicinal plant species with many beneficial properties. Thymol is the most important substance in the essential oil of this plant. Thymol is a natural monoterpene phenol with high anti-microbial, anti-bacterial, and anti-oxidant properties. Thymol in the latest research has a significant impact on slowing the progression of cancer cells in human. In this research, embryos were employed as convenient explants for the fast and effectual regeneration and transformation of T. ammi. To regenerate this plant, Murashige and Skoog (MS) and Gamborg's B5 (B5) media were supplemented with diverse concentrations of plant growth regulators, such as 6-benzyladenine (BA), 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), and kinetin (kin). Transgenic Trachyspermum ammi plants were also obtained using Agrobacterium-mediated transformation and zygotic embryos explants. Moreover, two Agrobacterium tumefaciens strains (EHA101 and LBA4404) harboring pBI121-TPS2 were utilized for genetic transformation to Trachyspermum ammi. Results According to the obtained results, the highest plant-regeneration frequency was obtained with B5 medium supplemented with 0.5 mg/l BA and 1 mg/l NAA. The integrated gene was also approved using the PCR reaction and the Southern blot method. Results also showed that the EHA101 strain outperformed another strain in inoculation time (30 s) and co-cultivation period (1 day) (transformation efficiency 19.29%). Furthermore, HPLC method demonstrated that the transformed plants contained a higher thymol level than non-transformed plants. Conclusions In this research, a fast protocol was introduced for the regeneration and transformation of Trachyspermum ammi, using zygotic embryo explants in 25–35 days. Our findings confirmed the increase in the thymol in the aerial part of Trachyspermum ammi. We further presented an efficacious technique for enhancing thymol content in Trachyspermum ammi using Agrobacterium-mediated plant transformation system that can be beneficial in genetic transformation and other plant biotechnology techniques.

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