Low levels of genetic diversity in red pine confirmed by random amplified polymorphic DNA markers

1992 ◽  
Vol 22 (9) ◽  
pp. 1332-1337 ◽  
Author(s):  
A. Mosseler ◽  
K.N. Egger ◽  
G.A. Hughes
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Black Spruce ◽  
Random Amplified Polymorphic Dna ◽  
Red Pine ◽  
Gene Markers ◽  
Island Populations ◽  
Oligonucleotide Primers ◽  
Low Levels

Random amplified polymorphic DNA (RAPD) markers were used to characterize genetic variation in disjunct Newfoundland populations of red pine (Pinusresinosa Ait.) for comparison with individuals from throughout the mainland range of red pine. Red pine demonstrated a largely monomorphic profile for 69 arbitrary oligonucleotide primers. DNA samples from white spruce (Piceaglauca (Moench) Voss) and black spruce (Piceamariana (Mill.) B.S.P.) that were screened together with red pine for 11 oligonucleotide primers showed abundant polymorphisms, confirming the genetic heterogeneity that characterizes these Boreal Zone spruces. Results with RAPD markers correspond with genetic diversity estimates using isozyme gene markers for both spruce species and red pine. RAPD markers provided further confirmation of low levels of genetic variation for a random sample of the red pine genome. A period of between 8000 and 10 000 years of isolation on the island of Newfoundland has resulted in very little detectable genetic differentiation of island populations from mainland populations, and the mainland populations have not recovered from losses of genetic diversity following a hypothesized genetic bottleneck that may have been experienced during glacial episodes of the Holocene. The low levels of genetic variation observed in red pine demonstrate the long time periods required for recovery following a loss of genetic diversity in long-lived, long-generation organisms like trees.

scholarly journals Genetic Similarity of Commerson’s Anchovy across Segara Anakan Cilacap Assessed Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Jurnal Biota ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Muhammad Khoerol Anam ◽  
Adi Amurwanto ◽  
Kusbiyanto Kusbiyanto ◽  
Hendro Pramono ◽  
M Husein Sastranegara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Differences ◽  
Population Genetic Study ◽  
Randomly Amplified Polymorphic Dna ◽  
High Genetic Diversity ◽  
Impressive Result

Segara Anakan areas can be divided into three different regions according to their salinity. Salinity differences suggested that Commerson’s anchovy population in that area can be divided into three subpopulations due to genetic differences. Genetic differences among subpopulation can be assessed through a population genetic study using random amplified polymorphic DNA. This study aims to evaluate the genetic variation and differences of Commerson's anchovy (Stolephorus commersonnii) collected at three different water salinities in Segara Anakan estuary Cilacap Indonesia. Total genomic DNA was isolated using the Chelex method. Genetic diversity and differences were assessed using RAPD markers and were analyzed statistically using an analysis of molecular variance, as implemented in Arlequin software.  The results showed that high genetic diversity was observed within the subpopulations. However, no significant genetic differences were observed among subpopulations which indicate genetic similarity. A high number of offspring are likely to cause high genetic variation within subpopulations.  Adult and larvae migration is the cause of genetics similarity across Segara Anakan. Another impressive result is that water salinity did not affect the genetic characteristic of Commerson,s anchovy. Genetic similarity of Commerson’s anchovy indicates that Segara Anakan forms a single genetic conservation unit.

scholarly journals Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

2005 ◽  
Vol 48 (4) ◽  
pp. 511-521 ◽  
Author(s):  
Leandro Eugênio Cardamoni Diniz ◽  
Claudete de Fátima Ruas ◽  
Valdemar de Paula Carvalho ◽  
Fabrício Medeiros Torres ◽  
Eduardo Augusto Ruas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Variability ◽  
Clustering Analysis ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Pcr Amplification ◽  
Restriction Digestion ◽  
Agronomic Features

The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.

Genetic diversity of Kensington mango in Australia

1996 ◽  
Vol 36 (2) ◽  
pp. 243 ◽  
Author(s):  
ISE Bally ◽  
GC Graham ◽  
RJ Henry
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Environmental Factors ◽  
Genetic Improvement ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Mangifera Indica ◽  
Genetic Variations ◽  
Oligonucleotide Primers

The genetic diversity of Kensington mangoes (Mangifera indica L.) was investigated using random amplified polymorphic DNA (RAPD) analysis. DNA was extracted from leaves of 27 'Kensington Pride', 2 'R2E2' and 1 seedling. RAPD analysis with 10 oligonucleotide primers allowed the scoring of 107 markers. The R2E2 trees (20% dissimilarity) and the seedling (10% dissimilarity) were distinct from the Kensington Pride. However, there was very little evidence of significant genetic variation within Kensington Pride selections. Fifteen of the selections were identical in all 107 markers. Only 2 selections, WEAN2 and ML2N1, differed by more than 5%. These plants provide the best options for use in genetic improvement of the Kensington Pride mango. Many of the differences found in Kensington mango orchards may be due to environmental factors not genetic variations.

scholarly journals Genetic Diversities within Camellia Species Confirmed by Random Amplified Polymorphic DNA (RAPD) Markers

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1105A-1105
Author(s):  
Lianghong Chen ◽  
Shizhou Wang ◽  
Mack Nelson
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Camellia Japonica ◽  
Average Percentage ◽  
Study Research ◽  
Camellia Reticulata ◽  
Camellia Species

In this study research was conducted to evaluate the feasibility of characterizing genetic variation within camellia species using random amplified polymorphic DNAs (RAPD) markers. Eight varieties of species Camellia japonica and four varieties of species Camellia reticulata, provided by the America Camellia Society, Fort Valley, Ga., were investigated. RAPD profiles generated by five selected 10-based random primers (out of 20 primers) exhibited distinct patterns of amplified bands for all 12 tested varieties. A total of 344 bands were produced among the eight varieties of species C. japonica, with an average of 8.6 bands, ranging from 220 to 2072 bp in size, scored per primer. Among the 344 amplified bands, 74.4% of the bands presented polymorphic. The four varieties of species C. reticulata produced a total of 180 markers, with an average percentage of 57.8% polymorphisms. The amplified bands were in the range of 236–1760 bp. An average of nine amplified bands was generated per primer. The large percentages of polymorphisms displayed among 12 varieties within the two different species indicate that the expected genetic diversity among varieties within camellia species existed. It was concluded that the RAPD molecular markers are capable of revealing appreciable levels of genetic variation within camellia species.

scholarly journals Evaluation of genetic diversity of Brassica napus germplasm from China and Europe assessed by RAPD markers

2011 ◽  
Vol 49 (No. 3) ◽  
pp. 106-113 ◽  
Author(s):  
Shengwu Hu ◽  
J. Ovesná ◽  
L. Kučera ◽  
V. Kučera ◽  
M. Vyvadilová
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Diversity Index ◽  
Random Amplified Polymorphic Dna ◽  
Pairwise Distance ◽  
Winter Rape ◽  
The Third ◽  
Best Fit

The genetic diversity and the relationships among rapeseed germplasm, including a collection of 20 Chinese, 25 Czech, 2 German, 2 French, and 1 English cultivars and breeding materials were evaluated using random amplified polymorphic DNA (RAPD) markers. A total of 79 different polymorphic amplification products were obtained using10 selected decamer primers. RAPDs revealed a significant level of polymorphism among the accessions. The diversity index (DI) ranged from 1.390 to 3.491, showing a sufficient potential of selected primers to differentiate among studied genotypes. Three different metrics were used to assess genetic diversity. The best fit between a priori knowledge about germplasm origin and a posteriori grouping was found using Hamman metrics. Cluster analysis based on Hamman pairwise distance comparison divided the studied accessions into three main clusters. The first group included only accessions fromChina, the second group only that fromEurope with the exception of Zhongshuang No. 2, a Chinese winter rape possessing European cultivars in the pedigree. The third group included accessions both fromChina andEurope. The results indicate the occurrence of a considerable genetic variation between Chinese and European accessions.

scholarly journals RAPD profiling in detecting genetic variation in Stellaria L. (Caryophyllaceae)

Genetika ◽  
2021 ◽  
Vol 53 (1) ◽  
pp. 349-362
Author(s):  
Xiaobang Peng ◽  
Majid Khayyatnezhad ◽  
Leila Ghezeljehmeidan
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Genetic Difference ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Data ◽  
Shannon Index ◽  
Neighbor Joining ◽  
Medicinal Value ◽  
Main Center

Stellaria species are common herbs, preferred humid mountainously slopes, but some grew in desert. Main center of diversification for Stellaria is Eurasia, with a center of distribution in the mountains of central Asia. Some species are also cosmopolitan. It is represented by 9 species in Iran. The genus has high medicinal value. To determine the genetic diversity and understand the species? limits within the Iranian Stellaria, we produced molecular data using 139 randomly collected plants representing 8 species from five provinces of Iran. A total of 122 reproducible bands were generated by 10 of 25 random amplified polymorphic DNA (RAPD) primers, with an average of 12.2 bands/primer and 33% polymorphism. Largest number of effective alleles (Ne), genetic diversity (H), and Shannon Index (I) were shown by S. media. Our data depicted highest similarity between S. media and S. pallida and lowest between S. media and S. graminea. S. pallida showed relatively low level of genetic variation. Finally, the Neighbor Joining (NJ) trees based on RAPD markers data divided the populations into two different clusters, indicating their genetic difference which is discussed in details.

scholarly journals Detection and analysis of genetic variation in Salicornieae (Chenopodiaceae) using random amplified polymorphic DNA (RAPD) markers

Taxon ◽  
1995 ◽  
Vol 44 (1) ◽  
pp. 53-63 ◽  
Author(s):  
T. Luque ◽  
C. Ruiz ◽  
J. Avalos ◽  
I. L. Calderón ◽  
M. E. Figueroa
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna

Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 995-999 ◽  
Author(s):  
H I Amadou ◽  
P J Bebeli ◽  
P J Kaltsikes
Keyword(s):  
Genetic Diversity ◽  
Cluster Analysis ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Bambara Groundnut ◽  
International Institute ◽  
Tropical Agriculture ◽  
Vigna Subterranea ◽  
Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (2) ◽  
pp. 201-210 ◽  
Author(s):  
F. N. Wachira ◽  
R. Waugh ◽  
W. Powell ◽  
C. A. Hackett
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Southeast Asia ◽  
Camellia Sinensis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Systematic Assessment ◽  
Tree Crop ◽  
Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

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