The use of unsaturated salt solutions to generate conifer needle water-release curves

1991 ◽  
Vol 21 (1) ◽  
pp. 53-57 ◽  
Author(s):  
N. J. Livingston ◽  
E. de Jong

Reversible changes in cell wall water content, elasticity, and osmotic adjustment are all important drought tolerance mechanisms. Pressure chambers have been used extensively to generate water-release curves from which these basic water relations parameters are derived. Major limitations of this technique include the considerable time required to generate water-release curves and the difficulty in avoiding damage to tissue during the process. A simple and practical technique is descried that overcomes these limitations and allows the routine and rapid generation of water-release curves. Needles were removed from hydrated black spruce (Piceamariana (Mill.) B.S.P.) shoots, weighed on a precision balance, and suspended above unsaturated salt solutions of known molality in sealed test tubes held at 5 °C. Eighteen solutions were used with water potentials ranging from −0.09 to −4.67 MPa. Once needles had equilibrated with air above the salt solution (8–30 h depending on needle age), they were weighed and oven-dried. Water-release curves were obtained by plotting paired values of needle relative water content and solution water potential. Typically, 35 curves were generated over 3 days compared with 7 curves generated using a pressure chamber over the same period. There was excellent agreement between methods in estimates of elastic modulus, osmotic potential, and apoplastic water content.

HortScience ◽  
2013 ◽  
Vol 48 (2) ◽  
pp. 216-221
Author(s):  
Ariningsun P. Cinantya ◽  
Fure-Chyi Chen ◽  
Yao-Chien Alex Chang

The popularity of the nobile-type dendrobium (Dendrobium nobile hybrids) has been increasing globally. More information regarding the effects of long-distance shipping, from producing countries to destination market countries, on the post-shipping plant performance is needed. In this study, two nobile-type dendrobium cultivars were subjected to simulated dark shipping (SDS) at various temperatures and durations. Changes in net CO2 uptake rate (Pn), chlorophyll fluorescence, and leaf relative water content after plants had been treated with SDS were investigated. Furthermore, shipped plants were vernalized to investigate the effect of dark shipping on the subsequent flowering quality. Dark shipping for 7 days at 15 °C did not affect the post-shipping photosynthetic performance of D. Lan Tarn Beauty. Increasing the shipping duration from 7 to 21 days increased the time required for Pn recovery from 1 to 12 days. Dendrobium Lan Tarn Beauty recovered its Pn within 4 days when shipped for 21 days at 10 °C, and this was prolonged to 11 days when the plants were shipped at 20 °C. Changes in Fv/Fm indicated that there was no marked damage to either cultivars, and the leaf relative water content was little affected by SDS. Dendrobium Lan Tarn Beauty and D. Lucky Girl shipped at 10 °C flowered 5 and 8 days earlier, respectively, compared with unshipped plants. Regardless of the shipping conditions, shipped D. Lucky Girl had a lower flower diameter and higher total flower count than unshipped plants. No differences were found in the number of nodes with flowers or the total flower count between shipped and unshipped D. Lan Tarn Beauty. Our study suggested that dark shipping for up to 21 days is possible for nobile-type dendrobiums. We recommend shipping temperatures of 10 to 15 °C to reduce the detrimental effects caused by long-term dark shipping.


2015 ◽  
Vol 37 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Nestor Martini Neto ◽  
Claudio José Barbedo

Seed storage at room temperature is an important and low-cost tool for ex situ conservation. However, the high rates of seed deterioration could reduce the potential for storage in this condition. Therefore, the knowledge of the suitable water content for this type of storage plays a critical role. This study aimed to assess the time required to stabilize the relative humidity (RH) in sealed flasks with saturated salt solutions, with or without the introduction of seeds of Caesalpinia echinata, as well as to assess the viability of these seeds stored in environments with different hygroscopic equilibrium. The results showed that 2 and about 12 days are needed to stabilize the RH, respectively, without or with the seeds. The amount of saturated salt solutions in this airtight environment influences both the speed to equilibrate the RH and the final values of the RH. Seeds of Caesalpinia echinata tolerate drying up to 5% water content (wet basis); however, the viability of these seeds at room temperature is maintained for short periods even at low water content.


2010 ◽  
Vol 7 (1) ◽  
Author(s):  
Saraswati Prabawardani

<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables /> <w:SnapToGridInCell /> <w:WrapTextWithPunct /> <w:UseAsianBreakRules /> <w:DontGrowAutofit /> <w:UseFELayout /> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--> <!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} --> <!--[endif]--> <p class="MsoNormal" style="text-align: justify;"><span style="font-size: 10pt;">The measurement of plant water status such as leaf water potential (LWP) and leaf relative water content (RWC) is important part of understanding plant physiology and biomass production. Preliminary study was made to determine the optimum amount of leaf abrasion and equilibration time of sweet potato leaf inside the thermocouple psychrometer chambers. Based on the trial, the standard equilibration time curve of a Peltier thermocouple for sweet potato leaf was between 2 and 3 hours. To increase the water vapour conductance across the leaf epidermis the waxy leaf cuticle should be removed or broken by abrasion. The result showed that 4 times leaf rubbings was accepted as the most effective way to increase leaf vapour conductance of sweet potato in the psychrometer chambers. In calculating the leaf relative water content, unstressed water of sweet potato leaves require 4 hours imbibition, whereas water stressed of sweet potato leaves require 5 to 6 hours to reach the saturation time. Either leaf water potential or relative water content can be used as a parameter for plant water status in sweet potato.</span><span style="font-size: 10pt;"> </span></p>


Weed Science ◽  
2015 ◽  
Vol 63 (4) ◽  
pp. 928-935 ◽  
Author(s):  
Sarah T. Berger ◽  
Jason A. Ferrell ◽  
Diane L. Rowland ◽  
Theodore M. Webster

Palmer amaranth is a troublesome weed in cotton production. Yield losses of 65% have been reported from season-long Palmer amaranth competition with cotton. To determine whether water is a factor in this system, experiments were conduced in 2011, 2012, and 2013 in Citra, FL, and in Tifton, GA. In 2011, infrequent rainfall lead to drought stress. The presence of Palmer amaranth resulted in decreased soil relative water content up to 1 m in depth. Cotton stomatal conductance (gs) was reduced up to 1.8 m from a Palmer amaranth plant. In 2012 and 2013 higher than average rainfall resulted in excess water throughout the growing season. In this situation, no differences were found in soil relative water content or cottongsas a function of proximity to Palmer amaranth. A positive linear trend was found in cotton photosynthesis and yield; each parameter increased as distance from Palmer amaranth increased. Even in these well-watered conditions, daily water use of Palmer amaranth was considerably higher than that of cotton, at 1.2 and 0.49 g H20 cm−2d−1, respectively. Although Palmer amaranth removed more water from the soil profile, rainfall was adequate to replenish the profile in 2 of the 3 yr of this study. However, yield loss due to Palmer amaranth was still observed despite no change ings, indicating other factors, such as competition for light or response to neighboring plants during development, are driving yield loss.


2021 ◽  
Vol 53 (4) ◽  
Author(s):  
Juan José Reyes-Pérez ◽  
Bernardo Murillo-Amador ◽  
Alejandra Nieto-Garibay ◽  
Luis G. Hernández-Montiel ◽  
Francisco H. Ruiz-Espinoza ◽  
...  

2012 ◽  
Vol 32 (2) ◽  
pp. 366-373 ◽  
Author(s):  
María Roberta Ansorena ◽  
María Victoria Agüero ◽  
María Grabriela Goñi ◽  
Sara Roura ◽  
Alejandra Ponce ◽  
...  

During postharvest, lettuce is usually exposed to adverse conditions (e.g. low relative humidity) that reduce the vegetable quality. In order to evaluate its shelf life, a great number of quality attributes must be analyzed, which requires careful experimental design, and it is time consuming. In this study, the modified Global Stability Index method was applied to estimate the quality of butter lettuce at low relative humidity during storage discriminating three lettuce zones (internal, middle, and external). The results indicated that the most relevant attributes were: the external zone - relative water content, water content , ascorbic acid, and total mesophilic counts; middle zone - relative water content, water content, total chlorophyll, and ascorbic acid; internal zone - relative water content, bound water, water content, and total mesophilic counts. A mathematical model that takes into account the Global Stability Index and overall visual quality for each lettuce zone was proposed. Moreover, the Weibull distribution was applied to estimate the maximum vegetable storage time which was 5, 4, and 3 days for the internal, middle, and external zone, respectively. When analyzing the effect of storage time for each lettuce zone, all the indices evaluated in the external zone of lettuce presented significant differences (p < 0.05). For both, internal and middle zones, the attributes presented significant differences (p < 0.05), except for water content and total chlorophyll.


1999 ◽  
Vol 34 (7) ◽  
pp. 1151-1157
Author(s):  
Adaucto Bellarmino de Pereira-Netto ◽  
Antonio Celso Novaes de Magalhães ◽  
Hilton Silveira Pinto

Tropical kudzu (Pueraria phaseoloides (Roxb.) Benth., Leguminosae: Faboideae) is native to the humid Southeastern Asia. Tropical kudzu has potential as a cover crop in regions subjected to dryness. The objective of this paper was to evaluate the effect of soil water depletion on leaflet relative water content (RWC), stomatal conductance (g) and temperature (T L) in tropical kudzu. RWC of waterstressed plants dropped from 96 to 78%, following a reduction in SWC from 0.25 to 0.17 g (H2O).g (dry soil)-1.Stomatal conductance of stressed plants decreased from 221 to 98 mmol.m-2.s-1, following the reduction in soil water content (SWC). The day after re-irrigation, g of water stressed plants was 15% lower than g of unstressed plants. Differences in T L between waterstressed and unstressed plants (deltaT L) rose linearly from 0.1 to 2.2ºC following progressive water deficit. RWC and T L of waterstressed plants paralled RWC and T L of unstressed plants the day after reirrigation. The strong decrease in SWC found in this study only induced moderate water stress in tropical kudzu. In addition, tropical kudzu recover rapidly from the induced water stress after the re-irrigation.


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