Interaction between gibberellin A4/7 and root-pruning on the reproductive and vegetative process in Douglas-fir. I. Effects on flowering

1985 ◽  
Vol 15 (2) ◽  
pp. 341-347 ◽  
Author(s):  
S. D. Ross ◽  
J. E. Webber ◽  
R. P. Pharis ◽  
J. N. Owens

In two seed orchard trials, profuse female flowering was induced in young, but ontogenetieally mature grafts of inherently poor-flowering clones (1979) and in 9-year-old seedling-origin trees of both good- and poor-flowering families (1981) of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) by the cultural treatment of root-pruning in conjunction with stem injections of the gibberellin A4 and A7 (GA4/7) mixture. Promotion of male flowering, however, was confined to the more sexually mature grafts. As an individual treatment in the 1981 study, root-pruning was more effective than GA4/7, particularly for the poor-flowering families which did not respond well to GA4/7 alone. The two treatments combined had a highly synergistic effect on both male and female flowering, the synergism being relatively greater for the poor-flowering than for the good-flowering families. Although GA4/7 was not tested alone on grafted propagules, its use with root-pruning enhanced an already significant increase in seed- and pollen-cone buds from root-pruning alone by 540 and 92%, respectively. These and subsequent trials have shown root-pruning +GA4/7 to be a most effective cone-bud enhancement treatment for use in young Douglas-fir breeding and seed production orchards.

1990 ◽  
Vol 20 (10) ◽  
pp. 1672-1675 ◽  
Author(s):  
V. J. Erickson ◽  
W. T. Adams

Proportions of outcrossed progeny (ti) in five ramets of five clones with variable floral phenology and pollen production were investigated in a Douglas-fir (Pseudotsugamenziesii var. menziesii (Mirb.) Franco) seed orchard. Estimates of ti[Formula: see text] were significantly heterogeneous among the five ramets, ranging in value from 0.50 to 1.07. Variation in [Formula: see text] corresponded well with variation among ramets in floral biology. Ramets that flowered much earlier than other individuals in the orchard and (or) had a high degree of synchrony in timing of male and female flowering produced the lowest [Formula: see text].


1982 ◽  
Vol 12 (2) ◽  
pp. 403-408 ◽  
Author(s):  
W. T. Adams

The mean number of pollen grains produced per pollen cone (P) ranged from 37 310 to 62 960 ([Formula: see text], SE = 1584) among eight seed-orchard clones of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) and correlated significantly (r = 0.87, p < 0.05) with the mean number of microsporophylls per cone but not significantly with pollen cone length. The number of pollen grains per milligram (G) also differed among clones (range 2323–3112; [Formula: see text], SE = 32) and correlated significantly and negatively (r = −0.86) with grain diameter. Although this study provides evidence for genetic variation in both P and G, the differences were relatively small. Adjusting pollen-cone counts for P would add little, if any, precision to estimates of pollen production in seed-orchard clones. If balanced clonal representation in pollen mixes is desired, equal weights of pollen would probably suffice for most breeding applications.


1985 ◽  
Vol 15 (2) ◽  
pp. 354-364 ◽  
Author(s):  
J. N. Owens ◽  
J. E. Webber ◽  
S. D. Ross ◽  
R. P. Pharis

The relative importance of cell division and cell elongation to shoot elongation and the anatomical changes in vegetative terminal apices were assessed for 9- and 10-year-old seedlings of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) in response to two effective cone-induction treatments, gibberellin A4/7 (GA4/7) and root-pruning (RP). Root-pruning was done in mid-April at the start of vegetative bud swelling and GA treatments were begun at vegetative bud flushing in mid-May and continued until early July. Shoot elongation before flushing resulted primarily from cell divisions and was not affected by the RP treatment. Shoot elongation after flushing resulted primarily from cell expansion which was reduced by RP treatments. Root-pruning significantly slowed mitotic activity, apical growth, and development of vegetative terminal buds from mid-June through mid-July. Apical growth then resumed during leaf initiation and the final number of leaf primordia initiated was not affected. This resulted in a delay of 2 to 4 weeks in the transition from bud-scale to leaf initiation. Retarded terminal vegetative apices anatomically resembled latent axillary apices but were never completely inhibited. GA + RP had the same effect as RP. GA4/7 alone had no effect on shoot or apical development. These results show that RP and GA + RP significantly retard shoot elongation and terminal bud development but still allow normal development of vegetative terminal buds. Retardation of bud development by a few weeks shifts the critical morphogenetic phase of transition from bud scale to leaf initiation to a later time when endogenous and environmental conditions may differ from the normal.


1981 ◽  
Vol 11 (1) ◽  
pp. 90-98 ◽  
Author(s):  
Stephen D. Ross ◽  
Richard F. Piesch ◽  
Franklin T. Portlock

Over 100-fold increases in numbers of seed cones and 6- to 24-fold increases in pollen cones were achieved for 7- and 8-year-old rooted ramets of sexually mature western hemlock (Tsugaheterophylla (Raf.) Sarg.) from stem injections of gibberellin A4/7 (GA4/7) mixtures and calcium nitrate fertilization. Female, but not male, flowering was also promoted significantly in nitrate-fertilized 6-year-old seedlings, both by stem injections (47% flowering) and foliar sprays (40% flowering) of GA4/7. Flower induction in 3-year-old potted seedlings required water stress in addition to GA4/7 foliar sprays (30% initiated seed cones and 7% pollen cones). The cones produced developed normally and set sound seeds. For mature ramets, flower promotion was roughly proportional to the logarithm of GA4/7 + GA9 concentration from 25 to 400 mg•L−1; calcium nitrate fertilization, though ineffective when used alone, significantly enhanced this response. Treatments of 6- and 12-week durations, beginning 24 May, were equally effective in promoting both male and female flowering. However, production of seed cones was only slightly enhanced and that of pollen cones not at all when treatment was delayed until 7 July (6 weeks), indicating the need to start GA applications before cone-bud differentiation has been completed (late June for pollen cones and mid-July for seed cones).


1977 ◽  
Vol 1 (2) ◽  
pp. 20-23 ◽  
Author(s):  
J. D. Gregory ◽  
C. B. Davey

Abstract Subsoiling in a loblolly pine seed orchard was studied in relation to female flowering, cone production, root growth, and soil bulk density. Subsoiling was conducted in late July 1973 in a nine-year-old seed orchard on sandy soil. The average number of female flowers per tree in 1974 and cones per tree in 1975 was considerably greater in the subsoiled plots than in the control plots. There was large tree-to-tree variability. The 1975 flower crop was not affected by the subsoiling treatments. Root excavations made two years after the subsoiling was done indicated vigorous generation and growth of new roots near the ends of cut roots. Soil bulk density was reduced in the upper soil horizons in and adjacent to the subsoiler slits. The stimulating effects on flowering by subsoiling are thought to result principally from the immediate physiological effects of root pruning.


1985 ◽  
Vol 15 (2) ◽  
pp. 348-353 ◽  
Author(s):  
J. E. Webber ◽  
S. D. Ross ◽  
R. P. Pharis ◽  
J. N. Owens

Shoot elongation and female flowering response were assessed for gibberellin A4/7 (GA4/7) and root-pruning (RP) treatments in 9- and 10-year-old Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings of families with good- and poor-flowering histories. In families with a poor-flowering history, stem injections of GA4/7 significantly enhanced elongation of third-whorl terminal shoots but produced no flowering response. In families with a good-flowering history, GA4/7 treatment had no effect on shoot elongation but did result in a significant increase in seed-cone buds. In contrast, root-pruning significantly retarded shoot growth in families with both good- and poor-flowering histories and was also the single most effective treatment for enhancing flowering. Combined, GA4/7 plus RP had a synergistic effect on flowering and GA4/7 partially overcame the inhibition of shoot growth caused by RP alone. These results are consistent with a hypothesis that exogenous and endogenous gibberellins are used preferentially for vegetative growth processes, with increased flowering occurring only after a threshold concentration of effector gibberellins is reached.


1986 ◽  
Vol 16 (2) ◽  
pp. 211-221 ◽  
Author(s):  
J. N. Owens ◽  
J. E. Webber ◽  
S. D. Ross ◽  
R. P. Pharis

The anatomy, mitotic frequency, size, and total insoluble carbohydrate histochemistry was studied in axillary apices from 9- and 10-year-old Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) trees after cone induction treatments of root-pruning and (or) stem injections of a gibberellin A4 and A7 (GA4/7) mixture. Axillary buds were initiated at the time of root-pruning, but root-pruning treatment had no effect on axillary bud initiation. Axillary apices from control and gibberellin-treated trees were similar and followed the normal sequence of bud-scale initiation, differentiation, and leaf initiation (described previously) and no cone buds differentiated. Early development of axillary apices from root-pruned and root-pruned, gibberellin-treated trees was normal, but development became retarded near the time of vegetative bud flush. Retarded apices were small with low mitotic frequency and developed many features characteristics of latent apices. Retardation of axillary apices continued until mid-July when normal development resumed and apices differentiated into reproductive buds or vegetative buds, or became latent. The trees in which the greatest retardation of apical development occurred during lateral shoot elongation produced the most cone buds. These results are discussed in relation to hypotheses proposed to explain how cultural and gibberellin treatments affect cone induction in the Pinaceae.


2017 ◽  
Vol 4 (8) ◽  
pp. 301-305
Author(s):  
Matouke M. Moise

The investigation of length-weight relationship and condition factor are very relevant indices to ascertain the health of fish in the water body. Length-weigh relationship and condition factor of Hyperopisus bebe (Lacepede, 1803) (Actinopterygii: Osteoglossiformes: Mormyridae) in River Galma, Zaria, Kaduna State, Nigeria, were assessed. The mean length and mean weigh in male and female were 26.703 cm +/- 0.51 cm and 25.32 cm +/- 0.898 cm; 158.2 g +/- 10.1 g and 95.91 g +/- 9.26 g, respectively. The exponent or growth pattern 'b' values for male and female were 2.009 and 2.176. The condition factor for male and female were 0.59 and 0.83, respectively. The negative allometric growth pattern and poor condition factor might be due to the poor water quality in the fish ecosystem and should be regarded with concern.


2014 ◽  
Vol 45 (125) ◽  
pp. 107
Author(s):  
Ignacio Madera Vargas

A fé dos pobres possui uma lógica interna que é necessário aprofundar para identificar o que dita lógica pode oferecer aos processos de nova evangelização na Igreja e da missão continental proposta por Aparecida. No interior da confissão de fé, compreendida em seus níveis epistemológico e ético, caracteriza-se a fé dos pobres em suas dimensões de minoridade, resistência e maravilha e suas relações com a fé e a esperança. Uma nova dinâmica ministerial emerge como necessária para uma nova evangelização do continente que inclui o ministério dos teólogos e teólogas em relação adulta com os pastores e o magistério da Igreja.ABSTRACT: The faith of the poor has an internai logic that is necessary to deepen in order to identify the contribution that logic can offer to the processes of the new evangelization in the Church and of the continental mission proposed by Aparecida. Within the confession of faith understood in its epistemological and ethical leveis, the faith of the poor is characterized in its dimensions of minority, resistance and wonder and its relationship with faith and hope. A new ministerial dynamic emerges as necessary for a new evangelization of the continent which includes the Ministry of theologians (male and female) in an adult relation with the pastors and the Magisterium of the Church. 


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