The effectiveness of mycelial slurries of mycorrhizal fungi for the inoculation of container-grown jack pine seedlings

1984 ◽  
Vol 14 (1) ◽  
pp. 140-142 ◽  
Author(s):  
R. M. Danielson ◽  
S. Visser ◽  
D. Parkinson
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Jack Pine ◽  
Considerable Time ◽  
Pine Seedlings ◽  
Inoculum Preparation

Mycelial slurries prepared from agar plates of ectomycorrhizal fungi were used to inoculate 7-week-old container-grown jack pine. Seven of 15 species formed mycorrhizae after 18 weeks and included Thelephoraterrestris Ehrhart ex Fr., Laccariaproximo Boudier, Hebeloma sp., Pisolithustinctoris (Pers.) Coker & Couch, Sphaerosporellabrunnea (Alb. & Schw. ex Fr.) Svrcek & Kubicka, Cenococcwngeophilum Fr., and an E strain (sensu Mikola) isolate. Species of Tricholoma, Suillus, Amphinema, and Hydnum failed to form mycorrhizae. The use of a mycelial slurry has the advantage of saving considerable time in inoculum preparation and should be useful for experimental purposes.

Host response to inoculation and behaviour of introduced and indigenous ectomycorrhizal fungi of jack pine grown on oil-sands tailings

1989 ◽  
Vol 19 (11) ◽  
pp. 1412-1421 ◽  
Author(s):  
R. M. Danielson ◽  
S. Visser
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Oil Sands ◽  
Host Response ◽  
Fold Increase ◽  
Growing Season ◽  
Jack Pine ◽  
Short Root ◽  
Replacement Process ◽  
Shoot Weight

Pinusbanksiana Lamb, seedlings were inoculated with nine mycorrhizal fungi and outplanted on an oil-sands containment dyke that had been amended with muskeg peat. After one growing season, E-strain (Complexipes), Hebeloma sp., Thelephoraterrestris Ehrh.:Fr., and Laccariaproximo Boudier each formed mycorrhizae with greater than 40% of the new short roots within 10 cm of the stem. Cenococcumgeophilum Fr., Pisolithustinctorius (Pers.) Coker & Couch, Astraeushygrometricus (Pers.) Morgan, Lactariusparadoxus Beardslee & Burlingham, and Sphaerosporellabrunnea (Alb. & Schw.:Fr.) Svrcek & Kubika each formed mycorrhizae with less than 6% of the short roots on egressed laterals. Of the introduced fungi, only E-strain was present in substantial quantities after 3 years. The quantity of short roots converted to mycorrhizae by indigenous fungi was 4, 33, and 72% after 1, 2, and 3 years, respectively. The change in mycorrhizal fungi appeared to be a noncompetitive replacement process, in which the original short root resident fungus died in the near absence of mycorrhizal fungi. At the end of the 3rd year, the major indigenous fungi converting short roots to mycorrhizae were E-strain, Tuber sp., Suillus-like spp., Myceliumradicisatrovirens Melin, and an unidentified basidiomycete. Inoculation with E-strain and Thelephoraterrestris resulted in a 2- to 3-fold increase in shoot weight after 2 years compared with uninoculated seedlings.

scholarly journals The effect of two ectomycorrhizal fungi, Paxillus involutus and Suillus tomentosus, and of Bacillus subtilis on Fusarium damping-off in jack pine seedlings

2005 ◽  
Vol 76 (2) ◽  
pp. 57-66 ◽  
Author(s):  
S.F. Hwang ◽  
P. Chakravarty ◽  
K.-F. Chang
Keyword(s):  
Bacillus Subtilis ◽  
Ectomycorrhizal Fungi ◽  
Seedling Survival ◽  
Jack Pine ◽  
Paxillus Involutus ◽  
In Vitro Growth ◽  
Damping Off ◽  
Pine Seedlings ◽  
Colony Forming Units

Two species of ectomycorrhizal fungi, Paxillus involutus and Suillus tomentosus, and a bacterial strain of Bacillus subtilis, were tested against Fusarium moniliforme, the causal agent of damping-off in jack pine (Pinus banksiana) seedlings. Both P. involutus and B. subtilis inhibited in vitro growth of F moniliforme. The culture filtrates of P. involutus and B. subtilis were toxic to F moniliforme, but chlamydospore formation of F moniliforme was observed. Greater jack pine seedling survival was observed when co-inoculated with P. involutus and B. subtilis than with F moniliforme alone. S. tomentosus neither inhibited in vitro growth of F moniliforme nor increased survival of jack pine seedlings against F moniliforme. F moniliforme reduced ectomycorrhiza formation on jack pine seedlings by P. involutus and S. tomentosus. The number of colony forming units of F moniliforme was significantly reduced when seedlings were inoculated with P. involutus and B. subtilis alone or in combination. S. tomentosus, on the other hand, did not reduce the number of colony forming units of F moniliforme. The suppression of F moniliforme growth by P. involutus and B. subtilis involved production of antifungal compounds.

Growth of containerized jack pine seedlings inoculated with different ectomycorrhizal fungi under a controlled fertilization schedule

1987 ◽  
Vol 17 (8) ◽  
pp. 840-845 ◽  
Author(s):  
J. Gagnon ◽  
C. G. Langlois ◽  
J. A. Fortin
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Nutrient Content ◽  
Jack Pine ◽  
Peat Moss ◽  
Available Phosphorus ◽  
Available Nitrogen ◽  
Available N ◽  
Pine Seedlings ◽  
Mycorrhiza Formation ◽  
Substrate Fertility

Containerized jack pine (Pinusbanksiana Lamb.) seedlings were grown in a peat moss – vermiculite substrate and inoculated at sowing with pure cultures of three ectomycorrhizal fungi. After 18 weeks of growth in the greenhouse, seedlings inoculated with Laccariabicolor had 97% of their short roots mycorrhizal, while those inoculated with Hebelomacylindrosporum had 36% of their rootlets mycorrhizal. No mycorrhizae were obtained with Rhizopogon sp. After both 12 and 18 weeks of growth with the fertilization schedule used, seedlings colonized with L. bicolor and H. cylindrosporum were significantly smaller than those inoculated with Rhizopogon sp. or control seedlings. After 18 weeks of growth, the available nitrogen (N) content of the substrate was considerably greater with L. bicolor inoculated seedlings than with control seedlings, whereas the available phosphorus (P) content of the substrate was the same for these two treatments. Also, after 18 weeks, seedlings mycorrhizal with L. bicolour had a. greater concentration (%) of N and P in their tissues than control seedlings; however, they contained fewer milligrams of N and P than control seedlings. From our results on substrate fertility of mycorrhizal seedlings, we state that it is possible to induce mycorrhiza formation of jack pine seedlings with L. bicolor when substrate fertility in available N and P is less than or equal to 39 and 60 ppm, respectively, and that it is possible to maintain L. bicolor mycorrhizae when N and P fertility is less than or equal to 24 and 28 ppm, respectively. From analysis of tissue nutrient content in mycorrhizal seedlings, it appears that the appropriate N and P concentrations to maintain mycorrhiza formation of jack pine seedlings with L. bicolor are less than or equal to 1.6 and 0.2%, respectively.

scholarly journals Ectomycorrhizal Fungi Associated with Pinus densiflora Seedlings under Flooding Stress

2021 ◽  
Vol 13 (8) ◽  
pp. 4367
Author(s):  
Yoonhee Cho ◽  
Shinnam Yoo ◽  
Myung Soo Park ◽  
Ji Seon Kim ◽  
Chang Sun Kim ◽  
...  
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Internal Transcribed Spacer ◽  
Pinus Densiflora ◽  
Red Pine ◽  
Fungal Communities ◽  
Thelephora Terrestris ◽  
Suillus Luteus ◽  
Flooding Stress ◽  
Pine Seedlings ◽  
Rhizopogon Luteolus

Flooding is an environmental stress for plants that not only limits aeration and nutrient acquisition, but also disturbs underground plant-associated fungal communities. Despite frequent flooding, red pine (Pinus densiflora) seedlings thrive in streamside environments. However, whether the compatible ectomycorrhizal fungi (EMF) of red pine are affected by natural flooding is unclear. As EMF are vital symbionts for the development of many trees and allow them to overcome various environmental stresses, in this study, the EMF species associated with red pine seedlings in a streamside environment in Korea were investigated after flooding. The EMF species in 47 seedlings collected from the streamside site were identified by observing their different morphotypes using internal transcribed spacer sequence analysis, and a total of 10 EMF species were identified. The EMF species diversity was lower than that in samples collected from a nearby forest analyzed as a control. The dominant EMF species of streamside seedlings included Amphinema spp., Rhizopogon luteolus, Suillus luteus, and Thelephora terrestris. This study could serve as a basis for investigating the mechanisms by which advantageous EMF aid plant development under flooding stress.

Distributions and Compositions of Brominated Diphenyl Ethers-209 in Pine Seedlings Inoculated with Ectomycorrhizal Fungi

2019 ◽  
Vol 230 (12) ◽  
Author(s):  
Zhugui Wen ◽  
Mengyu Chen ◽  
Huilong Lu ◽  
Shiqi Huang ◽  
Jincheng Xing ◽  
...  
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Brominated Diphenyl Ethers ◽  
Pine Seedlings ◽  
Diphenyl Ethers

Applied gibberellin A4/7 increases ovulate strobili production in accelerated growth jack pine seedlings

1981 ◽  
Vol 11 (3) ◽  
pp. 580-585 ◽  
Author(s):  
Robert A. Cecich
Keyword(s):  
Growth Conditions ◽  
Jack Pine ◽  
Fourfold Increase ◽  
Pine Seedlings ◽  
Accelerated Growth

Jack pine (Pinusbanksiana Lamb.) seeds were sown in October, January, and March, and the seedlings were cultured under accelerated growth conditions in a greenhouse. At biweekly intervals, from May 15 to August 15, they were transplanted to a nearby nursery and sprayed with GA4/7 or GA4/7 + NAA. The following spring a fourfold increase in flowering was noted in trees receiving either of the GA4/7 treatments. Trees in the March sowing did not flower. The data suggest that the increased flowering was caused by GA4/7-mediated differentiation of lateral long-branch primordia into ovulate strobili.

scholarly journals Ectomycorrhizal fungi mediate indirect effects of a bark beetle outbreak on secondary chemistry and establishment of pine seedlings

2015 ◽  
Vol 208 (3) ◽  
pp. 904-914 ◽  
Author(s):  
Justine Karst ◽  
Nadir Erbilgin ◽  
Gregory J. Pec ◽  
Paul W. Cigan ◽  
Ahmed Najar ◽  
...  
Keyword(s):  
Bark Beetle ◽  
Ectomycorrhizal Fungi ◽  
Indirect Effects ◽  
Secondary Chemistry ◽  
Pine Seedlings
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