The effect of sonication on the growth of some tree seeds
Air-dry seeds of jack pine (Pinusbanksiana Lamb.), red pine (P. resinosa Ait.), tamarack (Larixeuropaea L.), and white spruce (Piceaglauca (Moench) Voss) were subjected for 30 min to 1 MHz ultrasound at one of three intensities of ultrasound ranging from 0.5 to 6.0 W/cm2. Subsequent germination was assessed in terms of percent germination, mean daily germination (MDG), and peak velocity of germination (PV). From these, germination values (GV) were obtained. Only jack pine responded to the ultrasonic treatments by giving rise to higher MDG and GV values. The seeds were equally stimulated by all the intensities used. The rates and percent germination of the other tree species were unaffected by any of the sonication treatments. Seeds of jack pine were also sonicated at 25, 50, 100, 250, and 750 kHz at an intensity in the range of 0.5–1.0 W/cm2. None of these treatments affected the course of germination.Subsequent seedling growth of all the seeds in all treatment sets was also monitored by summing the total length of all the seedlings following 8 and 14 days of growth. The seedlings were divided into five length-range categories. The jack pine seedlings alone, sonicated at 1 MHz, had significantly more seedlings in the larger size group than the untreated seedlings.