Inhibition of Root- and Butt-decay Fungi by Extractives of Balsam Fir Root Wood

1974 ◽  
Vol 4 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Thomas E. Sterner
Keyword(s):  
Neutral Fraction ◽  
Balsam Fir ◽  
Decay Fungi ◽  
Chloroform Extraction

Growth of the root- and butt-decay fungi, Coniophoraputeana, Polyporusbalsameus, Scytinostromagalactinum, and Odontiabicolor, was significantly less on root centerwood than on stem heartwood of balsam fir. Also, root centerwood was much more resistant to decay than was stem heartwood. The inhibitory properties of the root wood were markedly reduced by chloroform extraction, and the concentrated extracts inhibited the growth of the decay fungi. Through bioassay, most of the inhibition was found to be attributable to four of the several spots appearing on chromatograms of the extract neutral fraction.

COLONIZATION BY DECAY FUNGI OF LIVING AND DEAD STEMS OF BALSAM FIR FOLLOWING ARTIFICIAL INJURY

1963 ◽  
Vol 41 (10) ◽  
pp. 1532-1534 ◽  
Author(s):  
D. E. Etheridge ◽  
L. A. Morin
Keyword(s):  
Balsam Fir ◽  
Decay Fungi

THE MICROBIOLOGICAL CONDITION OF WOOD OF LIVING BALSAM FIR AND BLACK SPRUCE IN QUEBEC

1967 ◽  
Vol 45 (7) ◽  
pp. 1003-1010 ◽  
Author(s):  
D. E. Etheridge ◽  
L. A. Morin
Keyword(s):  
Standard Method ◽  
Black Spruce ◽  
Balsam Fir ◽  
Sampling Techniques ◽  
Ecological Studies ◽  
Decay Fungi ◽  
The Past ◽  
Negative Results ◽  
Low Activity ◽  
Standard Techniques

Determinations of bacterial and fungal microfloras were made on 2% malt agar slants from wood samples of a living balsam fir, by following standard techniques for the isolation of decay fungi. Comparative studies with other microbiological sampling techniques and with different media have demonstrated (i) the general suitability of the standard method for ecological studies of wood-inhabiting microorganisms, and (ii) the validity of negative results obtained in previous isolation studies with the standard method. These findings reveal the pith column as a major center of bacterial activity in balsam fir, with relatively low activity recorded for the heartwood, and none at all in the sapwood. There is evidence that a similar situation exists in living black spruce, although the level of microbiological activity was much lower in the heartwood of this species, and bacteria almost nonexistent. Isolation studies conducted over the past 3 years in Quebec revealed that 40% of 132 living balsam fir trees and over 90% of 42 living black spruce trees sampled had sterile heartwood and sapwood. Retinocyclus abietis (Crouan) Groves and Wells was the only fungus isolated from both heart-wood and sapwood of the two species.

Application of RT-PCR for the Detection of Enteric Viruses in Oysters

1993 ◽  
Vol 27 (3-4) ◽  
pp. 49-53 ◽  
Author(s):  
L. A. Jaykus ◽  
R. De Leon ◽  
M. D. Sobsey
Keyword(s):  
Enteric Viruses ◽  
Rt Pcr ◽  
Chloroform Extraction ◽  
Cationic Detergent

Oyster samples processed by adsorption-elution-precipitation were seeded with poliovirus 1 and HAV, and cleaned and concentrated by Freon extraction (2X), PEG precipitation and chloroform extraction. Freon extraction resulted in recoveries of 63-76% for polio and 42-52% for HAV. PEG precipitation/chloroform extraction gave recoveries of 47-50% for polio and 15-19% for HAV. Treated extracts inhibited RT-PCR at 10−2 dilutions. Inhibitors were removed by treatment with the cationic detergent CTAB or Pro-Cipitate/UF adsorption-elution-concentration. Both treatments resulted in samples on which direct RT-PCR was possible. The CTAB procedure was able to detect 78 pfu of polio and 295 pfu of HAV. The Pro-Cipitate procedure was able to detect 70 pfu polio and 2.1×103 pfu HAV.

Effectiveness of Neem (Azadirachta indica A. Juss) Oil against Decay Fungi

2017 ◽  
Vol 5 (1) ◽  
pp. 48-51 ◽  
Author(s):  
Kanchan Rawat ◽  
◽  
Uttam Kumar Sahoo ◽  
Nagaraj Hegde ◽  
Awadhesh Kumar ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Schizophyllum Commune ◽  
Antimicrobial Properties ◽  
Fusarium Proliferatum ◽  
Wood Preservatives ◽  
Neem Oil ◽  
Environment Friendly ◽  
Decay Fungi ◽  
Natural Oils ◽  
Mycelia Growth

The enormous use of metallic wood preservatives has caused destructive impact on environment as well as human health. Therefore realizing the urgency of switching to Environment friendly options such as natural oils are being tested for their antimicrobial properties. The present study aimed at investigating potential of Neem oil against the growth ofdecaying fungi. The ability of Neem oil to inhibit mycelia growth of Schizophyllum commune, Fusarium oxysporum, Fusarium proliferatum, Coniophora puteana and Alternaria alternata was tested at different concentrations of 0.25, 0.50, 0.75, 1.0, 2.0, 4.0, 6.0, 8.0 and 10%. Results of the study revealed Neem oil concentrations above 2% were significantly inhibitory to all the tested fungi.

Sign in / Sign up

Export Citation Format

Share Document