The effect of nursery blackout application on Sitka spruce seedlings

1996 ◽  
Vol 26 (12) ◽  
pp. 2201-2213 ◽  
Author(s):  
C.D.B. Hawkins ◽  
A.M. Eastham ◽  
T.L. Story ◽  
R.Y.N. Eng ◽  
D.A. Draper

Sitka spruce (Piceasitchensis (Bong.) Carrière) seedlings were cultured using two nursery methods of blackout (short photoperiod) application: static treatment (constant day length of 14.28 h) and dynamic treatment (day length varying over length of experiment, with a mean of 14.28 h). Both methods when compared with a control (natural day length) reduced seedling height by inducing terminal bud set, increased seedling root weight, and decreased shoot to root and sturdiness ratios. Both static and dynamic blackout treatment seedlings had 26 and 30% lammas flush, respectively. Compared with control, both methods accelerated cold hardiness acquisition. After winter storage at −2 °C, treated seedlings flushed sooner than did control stock under light:dark temperatures of 25:20 °C. Under a cooler regime, 15:5 °C, differences among treatments were not significant. After planting in a common garden trial, terminal bud phenology had a range of treatment responses. Control seedlings flushed later and set bud sooner. Dynamic treatment seedlings' bud set phenology was nearest to that of controls. At a common garden trial and a reforestation site, blackout-treated seedlings had greater first-season terminal height increment. Planting check was observed for all treatments in the field during the second and third growing seasons. After five field seasons there was no height difference among treatments, and survival averaged 87%. Groundline stem diameter was never different among treatments. Biologically, the dynamic treatment is intermediate between the static and control treatments, but the static treatment is recommended because it is easier to apply in the nursery, and differences between the dynamic and static treatments were minimal.

2006 ◽  
Vol 84 (7) ◽  
pp. 1110-1121 ◽  
Author(s):  
J. Bradley St. Clair

Genetic variation in fall cold damage in coastal Douglas-fir ( Pseudotsuga menziesii (Mirb.) Franco var. menziesii ) was measured by exposing excised branches of seedlings from 666 source locations grown in a common garden to freezing temperatures in a programmable freezer. Considerable variation was found among populations in fall cold hardiness of stems, needles, and buds compared with bud burst, bud set, and biomass growth after 2 years. Variation in fall cold hardiness was strongly correlated (r = 0.67) with cold-season temperatures of the source environment. Large population differences corresponding with environmental gradients are evidence that natural selection has been important in determining genetic variation in fall cold hardiness, much more so than in traits of bud burst (a surrogate for spring cold hardiness), bud set, and growth. Seed movement guidelines and breeding zones may be more restrictive when considering genetic variation in fall cold hardiness compared with growth, phenology, or spring cold hardiness. A regional stratification system based on ecoregions with latitudinal and elevational divisions, and roughly corresponding with breeding zones used in Oregon and Washington, appeared to be adequate for minimizing population differences within regions for growth and phenology, but perhaps not fall cold hardiness. Although cold hardiness varied among populations, within-population and within-region variation is sufficiently large that responses to natural or artificial selection may be readily achieved.


1989 ◽  
Vol 19 (9) ◽  
pp. 1124-1130 ◽  
Author(s):  
Zeki Kaya ◽  
R. K. Campbell ◽  
W. T. Adams

The consequences for growth and phenology of early selection for height or its growth components were evaluated in 160 open-pollinated families of Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) from southwestern Oregon. Seedlings from two inland and two coastal populations (40 families each) were grown for two growing seasons in a common garden. Predicted response to selection suggests that risk of low juvenile–mature correlation and maladaptation with early selection would be less in the inland than in the coastal region. A phenological event that influences a common growth pattern seems to account for the difference in response. Early bud set in the 1st year was genetically correlated with larger overwintering buds in seedlings from both inland and coastal regions. These larger buds yielded a large increment of predetermined growth in the 2nd year, followed by little or no free growth and early bud set. Seedlings with late bud set in the 1st year had the converse pattern. Inland seedlings set buds much earlier on the average than did coastal seedlings; hence seedlings from the two regions had different growth patterns. Risks that can attend early selection for height generally would be decreased in both regions by selecting for predetermined growth, but several qualifications are discussed.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 548f-549
Author(s):  
Sunghee Guak ◽  
Lailiang Cheng ◽  
Leslie H. Fuchigami

Bench-grafted `Gala'/M26 apple trees were grown in pots and fertigated weekly with 150 ppm N starting from 10 May. N supply was terminated on 21 Aug., 18 Sept., and 9 Oct. for the early, mid, and late N cut-off treatment, respectively. Half of the trees in the early N cut-off treatment were sprayed twice with 3% urea at 6-day intervals in early October. Half of the trees in the mid and late N cut-off treatment were sprayed twice with 1000 ppm ABA at 5-day intervals in early and mid-October, respectively. Early N cut-off significantly enhanced terminal bud set, defoliation, and induction of cold acclimation compared to the late N cut-off. Foliar urea increased the reserve N level in all tissues of the early N cut-off trees, almost to the level of late N cut-off trees. Soluble protein concentrations were increased by foliar urea, which accounted for about 23%, 10%, and 17% of the increase in the total N concentration in bark, wood, and roots, respectively. ABA treatment enhanced leaf senescence and N mobilization in the mid and late N cut-off trees, but did not affect terminal bud set and cold hardiness.


Plant Disease ◽  
2020 ◽  
Author(s):  
Anna Wallis ◽  
Mario R. Miranda-Sazo ◽  
Kerik Cox

The adoption of mechanical thinning and pruning in commercial apple orchards has largely been limited by the risk of development and spread of fire blight. This devastating disease, caused by the bacterial pathogen Erwinia amylovora, may be transmitted by mechanical injury such as pruning, especially under warm, moist conditions conducive to bacterial growth, infection, and disease development. However, risk may be mitigated by avoiding highest risk times and applying a bactericide, such as streptomycin, following mechanical thinning or pruning. In ‘Gala’ and ‘Idared’ orchards, we evaluated the risk of fire blight development and spread following mechanical thinning early in bloom (20% bloom), when seasonal temperatures are cooler and there are few open flowers available for infection. In both orchards, we also evaluated the spread and development of fire blight by mechanical pruning in July and in August, before and after terminal bud set when shoot growth is slowed and less susceptible to infection. We also assessed the potential efficacy of a streptomycin or Bacillus subtilis biopesticide application following mechanical thinning and pruning to mitigate the spread of fire blight. In the ‘Gala’ orchard, disease never developed beyond the inoculated tree following thinning or pruning, which was unexpected for this highly susceptible cultivar. In the ‘Idared’ orchard, incidence of blossom or shoot blight from the point source, represented as relative area under the disease progress curve (rAUDPC) was rarely different for trees that received mechanical thinning or mechanical pruning compared to untreated trees, and was frequently eliminated or reduced when the antibiotic streptomycin or the B. subtilis biopesticide was applied within 24 h of mechanical thinning or pruning. For both thinning and pruning, incidence of fire blight dropped off quickly beyond the inoculated tree in the ‘Idared’ orchard and generally was not observed in trees beyond 10-15 m from the inoculated point source or predicted beyond 10 m by exponential and power law models fit to the disease progress curves. The results of this work demonstrate the low risk for fire blight development and spread by mechanical thinning and pruning when practiced under low-risk conditions—early in bloom for mechanical thinning, and after terminal bud set (in August) for mechanical pruning—especially when paired with a subsequent bactericide application. This study demonstrates the safe use of mechanical thinning and pruning in commercial apple production, corroborated by anecdotal evidence from apple growers in Western New York State.


1989 ◽  
Vol 19 (8) ◽  
pp. 1004-1013 ◽  
Author(s):  
Robert K. Campbell ◽  
William A. Pawuk ◽  
Arland S. Harris

Microgeographic genetic variation among populations of Sitka spruce on Mitkof Island in southeastern Alaska is described. In two common-garden environments, we evaluated genotypes of 208 parent trees from 114 locations in a 17 000-ha area. Two principal components accounted for most of the variation among locations in 11 traits measured to evaluate growth vigor and rhythm of 2-year-old seedlings. Regression analyses of factor scores derived from principal components revealed genetic gradients associated with elevation, slope, aspect, and west–east and north–south direction. Large amounts of additive genetic variation in factor scores occurred among trees within locations. When this variation within locations was used as a scale, variation among locations was also large. In an extreme case, locations differed in factor scores of the first principal component by about 3.0 units of the standard deviation of additive genetic variation in factor scores. Of the total differentiation in this case, elevational range (600 m) contributed 0.7 units of standard deviation, aspect contributed 0.9 units, and distance (16 km) from north central to southeastern parts of the island contributed 1.4 units.


2018 ◽  
Vol 48 (9) ◽  
pp. 1001-1006 ◽  
Author(s):  
Johanna Riikonen

Terminal bud set can be prevented by interrupting night with short pulses of light when the natural photoperiod is too short to maintain growth. Norway spruce (Picea abies Karst.) seedlings originating from 61°N and 64°N were grown in growth chambers under conditions that mimic growth conditions in a heated greenhouse in early spring in Finland (experiment 1) or under constant growth conditions (experiment 2). The seedlings were exposed to the following night interruption (NI) treatments using light-emitting diodes (LEDs) that generated red (R, peak at 660 nm) and far-red (FR, peak at 735 nm) wavelengths in 20 s pulses at 15 min intervals: (i) red light alone (R); (ii) R combined with FR (R + FR); and (iii) control (no NI treatment). The R + FR treatment was more effective in preventing terminal bud set than the R treatment. Seedling responses depended on the provenance and growth conditions. The R treatment reduced the proportion of seedlings with terminal buds in the 61°N seedlings and delayed bud set in the 64°N seedlings. The fluctuating growth conditions or longer dark period between the photoperiod and NI treatments reduced the efficiency of the R + FR treatment. A combination of R and FR LEDs with adequate light intensity and duration is suitable for intermittent NI treatment in Norway spruce seedlings.


2008 ◽  
Vol 38 (6) ◽  
pp. 1526-1535 ◽  
Author(s):  
Douglass F. Jacobs ◽  
Anthony S. Davis ◽  
Barrett C. Wilson ◽  
R. Kasten Dumroese ◽  
Rosa C. Goodman ◽  
...  

We tested effects of shortened day length during nursery culture on Douglas-fir ( Pseudotsuga menziesii var. menziesii (Mirb.) Franco) seedling development at dormancy release. Seedlings from a 42°N source were grown either under ambient photoperiods (long-day (LD)) or with a 28 day period of 9 h light : 15 h dark photoperiods (short-day (SD)). Seedlings were periodically removed from freezer storage from January to May. Sensitivity of plant tissues to cold temperatures was investigated via electrolyte leakage at nine test temperatures ranging from 2 to –40 °C. New root growth was assessed with rhizosphere temperatures of 10, 15, 20, and 25 °C. From 2 to –13 °C, there was no difference between treatments in cold hardiness. However, at or below –18 °C, LD seedlings exhibited higher indices of damage than SD seedlings. The LT50 (temperature at which 50% cell electrolyte leakage occurred) was consistently lower for SD than LD seedlings. Rhizosphere temperature differentially influenced new root proliferation: LD seedlings had greater new root production than SD seedlings at 20 °C, whereas the opposite response was detected at 10 °C. Our results confirm photoperiod sensitivity of Douglas-fir sources from relatively low (i.e., <45°N) latitudes. Increased spring cold hardiness and greater rooting at lower rhizosphere temperatures may improve field performance potential of SD-treated seedlings.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 449A-449
Author(s):  
Sunghee Guak ◽  
Leslie H. Fuchigami

Spring-grafted potted `Fuji'/M26 apple (Malus domestica Borkh.) trees were fertigated with Plantex (20N–10P–20K) weekly until 28 Aug., and sprayed with 1000 ppm abscisic Acid (ABA) two times at 5-day intervals in early September. Nitrogen concentrations of leaves, bark, wood, and root tissues were analyzed using near-infrared reflectance (NIR) spectroscopy at 20to 30-day intervals beginning in August. In general, during leaf senescence, the content of leaf nitrogen decreased and stem nitrogen increased. ABA enhanced leaf senescence and the mobilization of nitrogen from the leaves to the stem tissues. ABA significantly enhanced terminal bud set, endodormancy induction, and cold acclimation. Eventually, the controls attained the similar degree of nitrogen concentration in the stem, terminal bud set, endodormancy, and hardiness.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 557E-558 ◽  
Author(s):  
Christopher L. Owens ◽  
Eddie W. Stover

Early fruit production and control of tree size are important factors in the economic viability of high-density apple orchards. A horticultural tool permitting growers to induce terminal budset should provide greater control over the balance between vegetative growth and reproduction, increasing orchard production and profitability. With this goal, the experimental GA-biosynthesis inhibitor, BAS-125W, is being evaluated for effects on enhancing floral initiation and controlling tree size in young orchards. In nursery stock, the effect of inducing earlier terminal budset is also being studied for influence on storage carbohydrates and performance after planting. Studies in 1996 showed that 250 ppm BAS-125W induced terminal bud set on actively growing second-leaf `Macoun', `Delicious', and `Fuji' trees. Seven application dates from 17 June to 9 Sept. were compared to determine how time of treatment would effect degree and distribution of flowering the following year. Terminal budset typically occurred 2 weeks after application, with shoot growth resuming in 4 to 5 weeks. At two dates, treatment of growing tips only was compared with entire tree application to distinguish the direct effect of GA-inhibition on floral initiation from the effect of redistributing photosynthate. Treatment from 17 June to 29 July significantly reduced total annual shoot growth compared to the untreated controls, while later treatments had no significant effect on shoot length. Treatments of nursery stock with BAS-125W on 1 Sept. accelerated terminal bud set by at least 7 days compared to untreated controls of both `Fuji' and `Golden Delicious'. Effects of treatments on flowering and tree growth in 1997 will be discussed.


2021 ◽  
Author(s):  
Zhi Wang ◽  
Xiao-Qi Jiang ◽  
Xing-xing Cai ◽  
Qi-Yu Xia ◽  
Bao-Rong Lu

Abstract Background: Temperatures and photoperiods can profoundly affect plant growth and development and play vital roles in the local adaptation of plant species. Weedy rice (Oryza sativa f. spontanea) is a conspecific weed of cultivated rice, and it was found in the same rice fields (sympatry) of early and late rice-cultivation seasons in Leizhou, Guangdong Province of China. Generally, the phenological conditions, such as temperature and photoperiod, are different in the two seasons. Therefore, the early- and late-season weedy rice populations in the same rice fields provide a perfect system for estimating sympatric divergence in plant species. The previous study had demonstrated considerable genetic divergence between the early- and late-season weedy rice populations. Here, we designed in situ common garden experiments to estimate the phenotypical differences between the two-season weedy rice populations and disclose the local adaptation in weedy rice populations associated with their ambient temperature and photoperiod.Results: Distinct air temperature and day length variation patterns were recognized between the early and late rice-cultivation seasons, based on the 10-year historical climate data. More stressful conditions, indicated by low air temperature and long day length, were found for weedy rice growth in the early seasons. Noticeably, significant differences in plant heights, the number of tillers, flowering time, and reproductive traits were detected between the two-season weedy rice populations according to the early-season common garden experiment. The early-season populations showed evident higher plant heights, more tillers, and earlier flowering time than the late-season populations. However, such differences were not detected in the late-season common garden experiment. In addition, evident local adaptation represented by the traits such as plant heights, flowering time, and reproductive traits was only detected in the early-season weedy rice populations. The principal component analysis also showed clear population clusters between the two-season populations using the phenotypical data.Conclusions: This study provided clear evidence of phenotypic differentiation between the sympatric early- and late-season weedy rice populations, probably associated with the local adaptation to their ambient temperature and photoperiod. Our findings also have potential roles in facilitating the design of strategies for effective weedy rice control practices.


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