Micropropagation with a novel pattern of adventitious rooting in Formosan sweetgum

2005 ◽  
Vol 35 (12) ◽  
pp. 2775-2780 ◽  
Author(s):  
Jaroslav Ďurkovič ◽  
Viliam Pichler ◽  
Alexander Lux
Keyword(s):  
Shoot Elongation ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Shoot Cultures ◽  
Aerial Parts ◽  
Regenerated Plants ◽  
Aerial Roots

Cultures of the Formosan sweetgum (Liquidambar formosana Hance) were established from axillary buds of intact plants and from petiole segments of in vitro grown leaves. Petiole segments were generally slower than axillary buds to form multiple shoot cultures. Thidiazuron in combination with 6-benzylaminopurine produced on average up to 9.4 shoots per explant. Shoot elongation was significantly affected by the concentration of 6-benzylaminopurine, regardless of auxin addition to the medium. Adventitious rooting occurred up to 90%. An unexpected pattern of adventitious rhizogenesis was observed from the aerial parts of microshoots not in contact with the rooting medium, including leaf blades and petiole axils. In vitro adventitious root formation from the leaf blade was associated with parenchyma cells surrounding the main vein. The regenerated plants established ex vitro did not show any detectable morphological variation and no aerial roots were formed.

scholarly journals The Effect of Culture Physical Conditions and Explant Type and Source on the Growth and Multiplication of Ficus benjamina in Vitro

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873F-873
Author(s):  
N.S. Al-Khalifah ◽  
P.G. Alderson
Keyword(s):  
Light Intensity ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Shoot Length ◽  
Axillary Buds ◽  
Shoot Cultures ◽  
Variable Response ◽  
Ficus Benjamina ◽  
Chlorophyll Contents

Shoot cultures of Ficus benjamina cv. Cleo showed a variable response to light intensity in the range of 8 to 50 μmol–m–2–s–1. The highest leaf area occurred at 15 μmol–m–2–s–1 and shoot length and chlorophyll contents increased with the decrease of light intensity. Incubating explants for 8 weeks at 25 and 35C showed that shoot length increased at 35C; however, shoot multiplication, leaf number, root number, and callus weight were higher at 25C. Shoot-tip explants were superior to axillary buds when cultured or subcultured for shoot elongation, whereas axillary buds were better for shoot multiplication. Shoot tips with two or three small leaves appeared to be the best explants for fast growth and healthy cultures of F. benjamina.

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro Shoot Cultures of Tupistra albiflora K. Larsen

2018 ◽  
Vol 17 (5) ◽  
pp. 405-411
Author(s):  
Jiraporn PALEE
Keyword(s):  
Agar Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Shoots

To evaluate an efficient protocol for the micropropagation of Tupistra albiflora K. Larsen, the effects of N6-benzylaminopurine (BA) and naphthalene acetic acid (NAA) concentrations on multiple shoot and root induction were examined. In vitro shoots were used as the explant materials which were cultured on Murashige and Skoog (MS) agar medium supplemented with 0, 1, 2, 3 and 4 mg/L BA for 4 weeks to induce multiple shoots. It was found that the MS medium containing 3 mg/L BA induced 100 % shoot formation with the highest number of 3.2 shoots per explant (2.4-fold significantly higher than the control). For root induction, in vitro shoots were cultured on MS agar medium supplemented with 0, 1, 2, 3 and 4 mg/L NAA for 8 weeks. The results showed that the MS medium containing 1 mg/L NAA induced 100 % root formation with the highest number of 6.6 roots per explant (1.8-fold significantly higher than the control).

scholarly journals Introduction Pages

2019 ◽  
Vol 11 (1) ◽  
pp. I-VI
Author(s):  
Radu E. SESTRAS
Keyword(s):  
Date Palm ◽  
Disease Status ◽  
Shoot Elongation ◽  
Phoenix Dactylifera ◽  
Effective Control ◽  
Albino Rats ◽  
Normal Male ◽  
Shoot Cultures ◽  
African Mahogany

Notulae Scientia Biologicae (http://www.notulaebiologicae.ro), Issue 1, Volume 11, 2019: The papers published in this issue (http://www.notulaebiologicae.ro/index.php/nsb/issue/current) represent interesting novelties in different topics of life science. Among the exciting researches, we invite readers to find news about: The role of DNA Methylation in perennial plants; Peste des petits ruminants: Aetiology, pathology, immunology, disease status in Africa, diagnosis, control, prevention and treatment; Phytotherapy and polycyclic logging: implication on genetic multiplicity and diversity of African mahogany in tropical rainforest; Insight into re-emergence of cassava brown streak disease: the need to explore diverse approaches for effective control; Microbiological characterization of grilled meat “Tchatchanga” in Cotonou (Southern Benin): Enumeration, isolation and resistance profile of Staphylococcus aureus and Escherichia coli; Development of shoot cultures from leaf explant of Portulaca quadrifida L.; Evaluation of in vitro shoot elongation and rooting of date palm, and determination of physiological characteristics of regenerated plantlets; Maturation and germination of date palm (Phoenix dactylifera L.) somatic embryos; Hypoglycaemic and hypolipidemic effects of black brand of lipton tea (Camellia sinensis) on normal male albino rats.

scholarly journals In Vitro Micropropagation of Orchid (Vanda tessellata L.) from Shoot Tip Explant

1970 ◽  
Vol 17 ◽  
pp. 139-144 ◽  
Author(s):  
MS Rahman ◽  
MF Hasan ◽  
R Das ◽  
MS Hossain ◽  
M Rahman
Keyword(s):  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Root Induction ◽  
Ms Medium ◽  
Culture Techniques

Context: Orchid produces a huge number of minute seeds but the seeds can not germinate easily in nature due to the lack of endosperm in the seeds is an incompatibility barrier that limits its propagation in nature. Objectives: To develop in vitro culture techniques for quick propagation of Vanda tessellate, a commercially important orchid species. Materials and Methods: Shoot tips were used as experimental materials. The explants were surface sterilized and the shoot tips were excised. The isolated shoot tips were cultured in MS medium supplemented with different concentration and combinations of auxin and cytokinin. Results: The combination of 1.5 mgl-1 NAA and 1.0 mgl-1 BAP was proved to be the best medium formulation for multiple shoot formation as well as maximum shoot elongation. The single shoots were isolated from the multiple shoots and subcultured in MS medium having NAA and IBA individually and in combinations for root induction. Maximum root induction was obtained in MS agarified medium having 0.5 mgl-1NAA and 1.0 mgl-1IBA. The well rooted plantlets were hardened successfully in the potting mixture containing coconut husk, perlite, charcoal, brick pieces in the ratio of 2:1:1:1 and eventually established under natural condition.Conclusion: An efficient regeneration protocol for micropropagation in V. tessellata through shoot tip culture has been established.Key words: Shoot tip; micropropagation; orchid.DOI: 10.3329/jbs.v17i0.7122J. bio-sci. 17: 139-144, 2009

scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

scholarly journals Multiple Shoot Production In Vitro of the Tropical Timber Tree, Sentang (Azadirachta excelsa Linn.)

HortScience ◽  
1998 ◽  
Vol 33 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
T.K. Liew ◽  
C.K.H. Teo
Keyword(s):  
Sodium Hypochlorite ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Tropical Timber ◽  
Shoot Production ◽  
Timber Tree

Axillary buds from 5-month-old seedlings of Azadirachta excelsa Linn. were surface-sterilized twice with 1.35% (m/v) and 1.05% (m/v) of sodium hypochlorite for 25 and 15 minutes, respectively, before culturing on Murashige and Skoog (MS) medium containing combinations of BA and NAA. A combination of 4.4 μM BA + 0.5 μM NAA induced the most axillary buds to grow (eight per explant). Subsequent proliferation of the micropropagated shoots on this medium yielded abnormal shoots. The best medium for maximum proliferation of these micropropagated shoots contained 3.3 μM BA and 0.27 μM NAA. On this medium about four normal shoots were produced per explant. These findings indicate that two different media are needed for successful micropropagation of sentang. Chemical names used: N6-benzylaminopurine (BA); 1-naphthaleneacetic acid (NAA).

scholarly journals In vitro Mass Propagation from Shoot Tip of Dendrobium Red Bull - An Endangered Epiphytic Orchid

2018 ◽  
Vol 28 (2) ◽  
pp. 161-169 ◽  
Author(s):  
AA Mamun ◽  
MM Islam ◽  
M Ahmed ◽  
GC Ghosh
Keyword(s):  
Growth Regulators ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Good Growth ◽  
Multiple Shoot Induction ◽  
Best Response ◽  
Regenerated Plants ◽  
Shoot Number ◽  
Red Bull

An experiment was carried out to optimize the doses and combinations of growth regulators for in vitro propagation of Dendrobium Red Bull through shoot tip culture. The experiment was laid out according to completely randomized design with 15 treatments and three replications. For multiple shoot induction, newly grown shoot buds (keikies) were cultured on MS supplemented with various combinations of NAA and BAP. The regenerated plants were transferred to ex vitro in different substrates and acclimatized. Best response for the shoot length (21.19 mm) was obtained from the medium supplemented with 3.0 mg/l BAP with 1.5 mg/l NAA. Similarly maximum number of leaves was observed from the same combination at all the recorded days except 60 days, whereas the maximum shoot number (7.66) was obtained from the medium containing 3.0 mg/l BAP + 1.0 mg/l NAA. The shoots were then transferred to root inducing medium and well rooted plants were transferred to different substrates. Survivability of the regenerated plants varied from 60 to 92%. The substrate containing cocodust showed maximum survivability (92%) whereas minimum (60%) was observed from the substrate containing course sand. BAP and NAA proved to be good growth regulators for in vitro multiplication of Dendrobium. Plant Tissue Cult. & Biotech. 28(2): 161-169, 2018 (December)

scholarly journals Glycowithanolides accumulation in in Vitro Shoot Cultures of Indian Ginseng (Withania somnifera Dunal)

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ashok Ahuja ◽  
Devinder Kaur ◽  
Mallubhotla Sharada ◽  
Arun Kumar ◽  
Krishan Avtar Suri ◽  
...  
Keyword(s):  
Spectroscopic Data ◽  
Withania Somnifera ◽  
Renewable Resource ◽  
Multiple Shoot ◽  
Biologically Active ◽  
Shoot Cultures ◽  
Indian Ginseng ◽  
Multiple Shoot Cultures ◽  
In Vitro Shoot Cultures

Phytochemical investigations of multiple shoot cultures of selected accessions AGB002 and AGB025 of Withania somnifera. established in vitro utilizing shoot tip apices cultured on Murashige and Skoog's medium supplemented with BAP (1 mg/L) have been carried out. This has lead to isolation of four glycowithanolides viz. Withanoside IV (WSG-3), Withanoside VI (WSG-3A), Physagulin D (WSG-P) and Withastraronolide (WSC-O). The structures of these have been confirmed on the basis of spectroscopic data. Multiple shoot cultures could be an alternative renewable resource for production of these biologically active molecules.

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