An inexpensive portable freezer for in situ freezing in the field

2002 ◽  
Vol 32 (12) ◽  
pp. 2160-2168 ◽  
Author(s):  
Yves Dubuc ◽  
Jean Dubuc ◽  
Francine J Bigras

A portable freezer was developed to apply frost to branches of large trees to study their growth and recuperation after frost application under natural conditions. The freezer measures 37.5 × 63.5 × 31.5 cm and weighs approximately 3 kg. It consists of two compartments, a freezing compartment and a dry ice compartment. The portable freezer provides a ramp-and-soak freezing pattern using a programmable controller. The nonfreezing temperature plateaus can be set from 1 to 6°C and maintained for 0 to 12 h. The cooling and warming rates can be programmed from 1 to 12°C·h–1. Test temperatures can be maintained for a period of time ranging from 0 to 12 h at set temperatures. Freezers were tested without samples under controlled conditions at ambient temperatures of 0, –5, –10, 5, 15, 20, and 25°C. Under these conditions, the cooling and warming rates showed a deviation of less than ±1°C·h–1 at a set rate of 2°C·h–1. The freezer provides test temperatures as low as –38°C and –47°C at ambient temperatures of 20 and –10°C, respectively. Freezers were also tested under field conditions on attached branches in mature white spruce (Picea glauca (Moench) Voss) trees under hardening conditions.

Author(s):  
Juliana Marcia Rogalski ◽  
Daniela da Silva ◽  
Júlio Tagliari Balestrin ◽  
Kaliandra Severina Mattei ◽  
Angela Julia Dorn ◽  
...  

The objective of this study was to evaluate the seed germination of Tropaeolum pentaphyllum Lam., an endangered geophyte native of southern Brazil with agriculture potential due its edible tubers. Two experiments were carried out in 2017: germination of T. pentaphyllum under natural conditions and under controlled conditions. In the experiments under natural and controlled conditions, the germination of 1,100 and 100 seeds was evaluated, respectively. In the experiment under controlled conditions, two treatments (natural light and dark) were evaluated and each treatment contained 50 seeds. In the natural conditions experiment, in 2018 (first year), of the 1,100 seeds, 5.6% germinated and 76.5% persisted in the soil; and in 2019 (second year), 5.3% of the seeds germinated and no seeds remained in the soil. In natural conditions experiment, 10.9% of seeds germinated over two years. In controlled conditions experiment, in 2018 the germination in dark was higher (48%) compared to natural light (18%); and in 2019, despite the absence of statistical differences, the dark showed a higher value of germination (12%) in relation to natural light (6%). Over two years, dark treatment showed higher germination (60%) compared to natural light (24%) and no seeds remained in the substrate. The production of tubers in plants obtained by seed germination was 98.4% and 100%, in natural and controlled conditions, respectively. The highest germination rates occurred under dark conditions indicating that the species is preferential negative photoblastic. In addition, this species forms a seed bank in the soil, in which the seeds remained for a maximum of two years. The seed germination can contribute to the genetic diversity of crops and the production of seed-tubers, decreasing the collection of tubers in situ, contributing to the conservation and agricultural use of T. pentaphyllum.


1996 ◽  
Vol 72 (2) ◽  
pp. 176-180 ◽  
Author(s):  
David W. Ip ◽  
Irene L. Pines ◽  
A. Richard Westwood

Mature and over mature white spruce [Picea glauca (Moench) Voss] repeatedly defoliated by spruce budworm (Choristoneura fumiferana Clem.) in eastern Manitoba, were investigated for variation in moisture content (MC) which is known to influence pulping quality. In the fall of 1991, 100 trees in the Abitibi-Price Inc. Forest Management Licence area were selected randomly in two size classes and four defoliation classes. Heartwood and sapwood MC (% dry weight) was measured in 5-cm thick disks cut at 2.5 to 5.0 m intervals for small and large trees, respectively. Living trees with more sapwood than heartwood had a mean MC of 117%, and those with more heartwood than sapwood had a mean MC of 77%. Both of these groups contained sufficient overall moisture (> 45%) for pulping. The repeated defoliation did not affect the overall MC in live trees. Mean MC of trees killed by spruce budworm defoliation was 42% but this level varied with diameter and height above ground: > 45% in stems greater than 36 cm diameter inside bark, 30–60% in sections 17–36 cm, and < 45% in sections less than 17 cm. A minimum DBH of 30 cm is suggested as a criterion for accepting dead trees. Key words: insect defoliation impact, wood moisture content, sapwood, heartwood, dead tree fibre, Manitoba


1997 ◽  
Vol 129 (2) ◽  
pp. 291-318 ◽  
Author(s):  
Robert K. Lawrence ◽  
William J. Mattson ◽  
Robert A. Haack

AbstractSynchrony of insect and host tree phenologies has often been suggested as an important factor influencing the susceptibility of white spruce, Picea glauca (Moench) Voss, and other hosts to the spruce budworm, Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae). We evaluated this hypothesis by caging several cohorts of spruce budworm larvae on three white spruce populations at different phenological stages of the host trees, and then comparing budworm performance with host phenology and variation of 13 foliar traits. The beginning of the phenological window of susceptibility in white spruce occurs several weeks prior to budbreak, and the end of the window is sharply defined by the end of shoot growth. Performance was high for the earliest budworm cohorts that we tested. These larvae began feeding 3–4 weeks prior to budbreak and completed their larval development prior to the end of shoot elongation. Optimal synchrony occurred when emergence preceded budbreak by about 2 weeks. Larval survival was greater than 60% for individuals starting development 1–3 weeks prior to budbreak, but decreased to less than 10% for those starting development 2 or more weeks after budbreak and thus completing development after shoot elongation ceased. High performance by the budworm was most strongly correlated with high levels of foliar nitrogen, phosphorous, potassium, copper, sugars, and water and low levels of foliar calcium, phenolics, and toughness. These results suggest that advancing the usual phenological window of white spruce (i.e. advancing budbreak prior to larval emergence) or retarding budworm phenology can have a large negative effect on the spruce budworm’s population dynamics.


Botany ◽  
2011 ◽  
Vol 89 (5) ◽  
pp. 323-338 ◽  
Author(s):  
A. Tsuneda ◽  
M.L. Davey ◽  
R.S. Currah

An endoconidial, black meristematic taxon Atramixtia arboricola gen. et. sp. nov. (Dothideales) from the black subicula found on twigs of declining white spruce, Picea glauca (Moench) Voss, in Alberta is described. It is morphologically distinguishable from other endoconidial taxa by the conidioma composed of clumps of endoconidial conidiogenous cells, scattered meristematically dividing cells, dematiaceous hyphae, abundant brown, granular matrix materials, and sometimes plant tissue. Endoconidia also occur in conidiogenous cellular clumps that are not organized into a conidioma but develop directly from stromatic cells on the bark. In culture, it forms similar endoconidial conidiomata and also a mycelial, blastic synanamorph that superficially resembles Hormonema . Atramixtia arboricola is a member of the Dothideales and shows phylogenetic affinities to a clade of conifer-stem and -needle pathogens, including Sydowia and Delphinella , although no teleomorph was found either on the natural substrate or in culture. It has not been determined whether A. arboricola is pathogenic to its host, but the occurrence of abundant intracellular hyphae in the host periderm suggests that the fungus is at least parasitic.


2004 ◽  
Vol 34 (7) ◽  
pp. 1538-1542 ◽  
Author(s):  
Heidi Steltzer

Soil carbon (C) and nitrogen (N) pools were measured under the canopy of 29 white spruce (Picea glauca (Moench) Voss) trees and in the surrounding tundra 3 and 6 m away from each tree at three sites of recent forest expansion along the Agashashok River in northwestern Alaska. The aim was to characterize the potential for forest expansion to lead to increased soil C pools across diverse tundra types. Soil C beneath the trees correlated positively with tree age, suggesting that tree establishment has led to C storage in the soils under their canopy at a rate of 18.5 ± 4.6 g C·m–2·year–1. Soil C in the surrounding tundra did not differ from those under the trees and showed no relationship to tree age. This characterization of the soil C pools at the 3-m scale strengthens the assertion that the pattern associated with the trees is an effect of the trees, because tree age cannot explain variation among tundra sampling locations at this scale. Potential mechanisms by which these white spruce trees could increase soil C pools include greater production and lower litter quality.


Author(s):  
Andrei Lapenis ◽  
George Robinson ◽  
Gregory B. Lawrence

Here we investigate the possible<sup></sup> future response of white spruce (Picea glauca) to a warmer climate by studying trees planted 90 years ago near the southern limit of their climate tolerance in central New York, 300 km south of the boreal forest where this species is prevalent. We employed high-frequency recording dendrometers to determine radial growth phenology of six mature white spruce trees during 2013-2017. Results demonstrate significant reductions in the length of radial growth periods inversely proportional to the number of hot days with air temperature exceeding 30 oC. During years with very hot summers, the start of radial growth began about 3 days earlier than the 2013-2017 average. However, in those same years the end of radial growth was also about 17 days earlier resulting in a shorter (70 versus 100 day), radial growth season. Abundant (350-500 mm) summer precipitation, which resulted in soil moisture values of 20-30% allowed us to dismiss drought as a factor. Instead, a likely cause of reduced radial growth was mean temperature that exceeded daily average of 30<sup> o</sup>C that lead to photoinhibition.


Sensors ◽  
2018 ◽  
Vol 18 (12) ◽  
pp. 4406 ◽  
Author(s):  
Rafael Sola-Guirado ◽  
Sergio Bayano-Tejero ◽  
Antonio Rodríguez-Lizana ◽  
Jesús Gil-Ribes ◽  
Antonio Miranda-Fuentes

Canopy characterization has become important when trying to optimize any kind of agricultural operation in high-growing crops, such as olive. Many sensors and techniques have reported satisfactory results in these approaches and in this work a 2D laser scanner was explored for measuring canopy trees in real-time conditions. The sensor was tested in both laboratory and field conditions to check its accuracy, its cone width, and its ability to characterize olive canopies in situ. The sensor was mounted on a mast and tested in laboratory conditions to check: (i) its accuracy at different measurement distances; (ii) its measurement cone width with different reflectivity targets; and (iii) the influence of the target’s density on its accuracy. The field tests involved both isolated and hedgerow orchards, in which the measurements were taken manually and with the sensor. The canopy volume was estimated with a methodology consisting of revolving or extruding the canopy contour. The sensor showed high accuracy in the laboratory test, except for the measurements performed at 1.0 m distance, with 60 mm error (6%). Otherwise, error remained below 20 mm (1% relative error). The cone width depended on the target reflectivity. The accuracy decreased with the target density.


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