Differences in needle morphology between blister rust resistant and susceptible western white pine stocks

2001 ◽  
Vol 31 (11) ◽  
pp. 1880-1886 ◽  
Author(s):  
Kwan-Soo Woo ◽  
Lauren Fins ◽  
Geral I McDonald ◽  
Maurice V Wiese
Keyword(s):  
Rust Fungus ◽  
Seed Orchard ◽  
Contact Angles ◽  
Pinus Monticola ◽  
White Pine ◽  
Blister Rust ◽  
Needle Morphology ◽  
Genetic Stocks ◽  
Western White Pine ◽  
Stomatal Length

Needle traits were evaluated on three groups of western white pine (Pinus monticola Dougl.) seedlings: four open-pollinated families that ranked high for the "reduced needle lesion frequency" type of resistance to blister rust; four blister rust susceptible families; and two bulk lots from a seed orchard selected for blister rust resistance. No statistically significant differences were found for most traits in pairwise comparisons among the three groups. However, needles of susceptible families had significantly wider and larger stomata (greater area) than did those of resistant families and seed orchard lots; their stomata were also rounder (smaller ratio of stomatal length to width) than those of the seed orchard lots. Needles of the resistant stocks were significantly shorter than those from seed orchard bulks. Contact angles of water droplets on adaxial needle surfaces were also significantly larger on resistant families compared with the other genetic stocks. Results suggest the possibility of some threshold stomatal size and (or) critical stomatal shape related to infection by the blister rust fungus, Cronartium ribicola J.C. Fisch. ex Rabenh., and possible differences among the groups in wax chemistry and (or) surface textures, both of which may alter behavior of blister rust germ tubes and (or) be altered by blister rust infection.

Resistance to Cronartium ribicola in Pinus monticola: reduced needle-spot frequency

1980 ◽  
Vol 58 (5) ◽  
pp. 574-577 ◽  
Author(s):  
R. J. Hoff ◽  
G. I. McDonald
Keyword(s):  
Mating System ◽  
Rust Fungus ◽  
Low Frequency ◽  
Resistance Factor ◽  
Cronartium Ribicola ◽  
Pinus Monticola ◽  
Horizontal Resistance ◽  
White Pine ◽  
Blister Rust ◽  
Western White Pine

Low frequency of needle spots caused by the blister rust fungus (Cronartium ribicola J. C. Fisch. ex Rabenh.) in western white pine (Pinus monticola Dougl.) appears to be an expression of a horizontal resistance factor in secondary needles. Heritability averaged 37% for two sets of a 4 tester × 10 candidate mating system. We discuss the implications of these results with respect to developing varieties of western white pine resistant to blister rust.

scholarly journals White Pine Blister Rust in British Columbia III. Effects on the Gene Pool of Western White Pine

1985 ◽  
Vol 61 (6) ◽  
pp. 484-488 ◽  
Author(s):  
R. S. Hunt ◽  
J. F. Manville ◽  
E. von Rudloff ◽  
M. S. Lapp
Keyword(s):  
Pacific Northwest ◽  
Gene Pool ◽  
Resistance Mechanisms ◽  
Geographic Range ◽  
White Pine Blister Rust ◽  
Pinus Monticola ◽  
White Pine ◽  
Blister Rust ◽  
The Pacific ◽  
Western White Pine

Cluster analyses of relative terpene abundance in foliage of western white pine (Pinus monticola Dougl.) trees from throughout the Pacific Northwest geographic range of the species were produced. Terpene patterns were randomly distributed among populations; no geographic or site trends were evident. Although blister rust is devastating to stands, the gene pool is widely distributed and may well be preserved without establishing gene banks.About 40-50 trees selected at random would yield offspring with nearly all possible terpene patterns characteristic of the species and would thus constitute a broad genetic base. Therefore seed orchards do not necessarily need to be composed of many individuals, rather, they should contain highly selected individuals with multiple desirable traits including multiple blister rust resistance mechanisms. Key words: terpenes, dendrogram

The relative susceptibility of needle- and stem-derived white pine tissue cultures to artificial inoculation with mycelium of Cronartium ribicola

1969 ◽  
Vol 47 (11) ◽  
pp. 1789-1790 ◽  
Author(s):  
A. E. Harvey ◽  
J. L. Grasham
Keyword(s):  
Rust Fungus ◽  
Cronartium Ribicola ◽  
Tissue Cultures ◽  
Pinus Monticola ◽  
White Pine ◽  
Relative Susceptibility ◽  
Blister Rust ◽  
Leaf Mesophyll ◽  
Leaf Tissues ◽  
Artificial Inoculations

Tissue cultures of Pinus monticola Dougl. derived from stem cortex and leaf tissues were found susceptible to artificial inoculations with mycelium from the blister rust fungus (Cronartium ribicola Fisch. ex Rabenh.). Tissue cultures from leaf mesophyll grew slower and were colonized more rapidly by this fungus than those derived from stem cortex.

White Pine Blister Rust

2001 ◽  
Vol 2 (1) ◽  
pp. 10 ◽  
Author(s):  
Otis C. Maloy
Keyword(s):  
North America ◽  
Rust Fungus ◽  
White Pine Blister Rust ◽  
Alternate Host ◽  
White Pine ◽  
Management Tools ◽  
Disease Cycle ◽  
Blister Rust ◽  
Western White Pine ◽  
Pine Stands

White pine blister rust is probably the most destructive disease of five-needle (white) pines in North America. The rust fungus cannot spread from pine to pine but requires an alternate host, Ribes species, to complete the disease cycle. Several management tools might enable the reestablishment of western white pine stands. Accepted for publication 20 September 2001. Published 24 September 2001.

Pollination and seed production in western white pine

2007 ◽  
Vol 37 (2) ◽  
pp. 260-275 ◽  
Author(s):  
John N. Owens ◽  
Danilo D. Fernando
Keyword(s):  
Seed Production ◽  
Seed Orchard ◽  
Optimal Time ◽  
Pinus Monticola ◽  
White Pine ◽  
Clonal Seed Orchard ◽  
Seedling Seed Orchard ◽  
Western White Pine ◽  
Cone Development ◽  
Filled Seeds

The reproductive biology of western white pine ( Pinus monticola Dougl. ex D. Don) at the Saanich seed orchard (SSO; a coastal seedling seed orchard) and the Kalamalka seed orchard (KSO; an interior clonal seed orchard) is described. Seed-cone development and morphology determine seed potential and affect filled seed production. Seed potential was high, commonly over 200 seeds per cone and filled seeds per cone averaged 50–60 at both orchards in 1999 from open pollinations combined with operational supplemental mass pollinations (OP-SMP). Filled seeds per cone at SSO in 2004 averaged 115 with open pollinations and OP-SMP. About 30% of ovules aborted before pollination. Another 25% aborted probably because of self-incompatibility at fertilization. Seed efficiency (SEF) was only 5% at KSO with open pollination but increased to 30% with OP-SMP. SEF was the same at SSO with or without OP-SMP. Reproductive success was very low at KSO without OP-SMP but increased to about 10%, similar to that of SSO, with OP-SMP. The optimal time for control pollinations was at cone stages 4 and 5 and optimal amount of pollen per pollination bag was 0.3–0.4 mL. Western white pine is a very good seed producer and can be easily managed in seed orchards to give very high seed production per cone and per tree.

scholarly journals Cloning and Characterization of a Putative Antifungal Peptide Gene (Pm-AMP1) in Pinus monticola

2006 ◽  
Vol 96 (2) ◽  
pp. 164-170 ◽  
Author(s):  
A. K. M. Ekramoddoullah ◽  
J.-J. Liu ◽  
A. Zamani
Keyword(s):  
Defense Response ◽  
Rust Fungus ◽  
Antifungal Protein ◽  
Pinus Monticola ◽  
White Pine ◽  
Macadamia Integrifolia ◽  
Blastx Search ◽  
Western White Pine ◽  
Peptide Gene

We have been working on proteins that are involved in the defense response of western white pine (WWP) (Pinus monitcola) to the blister rust fungus Cronartium ribicola. Our objective was to identify candidate genes that could be used for improving resistance of WWP to this rust pathogen. During proteomic analysis of bark proteins extracted from WWP trees exhibiting slow-canker-growth (SCG) resistance, a 10.6-kDa peptide, termed Pm-AMP1, was found to be enriched at the receding canker margin. The cDNA encoding this peptide was cloned and characterized. A BLASTX search revealed that the Pm-AMP1 encoded by its cDNA has a 50% homology with MiAMP1, a broad-spectrum antifungal protein isolated from Macadamia integrifolia. Based on the deduced amino acid sequence, an antibody was produced against the Pm-AMP1. Immunochemical quantification of the Pm-AMP1 in bark samples of susceptible WWP trees revealed this protein to be barely detectable in the cankered tissues, but occurring in higher concentrations in healthy tissues away from canker margins. Foliage of SCG-resistant trees contained higher concentrations of the Pm-AMP1 than foliage from susceptible cankered trees. Both wounding and methyl jasmonate treatment of WWP needles induced the expression of this protein, further supporting its putative role as a defense response protein.

A protein associated with frost hardiness of western white pine is up-regulated by infection in the white pine blister rust pathosystem

1998 ◽  
Vol 28 (3) ◽  
pp. 412-417 ◽  
Author(s):  
Abul KM Ekramoddoullah ◽  
Joanne J Davidson ◽  
Doug W Taylor
Keyword(s):  
Frost Hardiness ◽  
White Pine Blister Rust ◽  
Pinus Monticola ◽  
White Pine ◽  
Blister Rust ◽  
Pine Seedlings ◽  
Western White Pine ◽  
Pine Trees ◽  
High Level ◽  
Stem Cankers

A 19-kDa protein, Pin m III, was recently shown to be associated with overwintering and frost hardiness of western white pine (Pinus monticola Dougl. ex D. Don) seedlings. Here, we report that this protein is up-regulated by the fungus Cronartium ribicola Fisch, the causal agent of white pine blister rust in western white pine trees. Between 1991 and 1994, bark samples of mature western white pine trees (resistant with no stem cankers and susceptible with stem cankers) were collected in winter, spring, and fall. Proteins were extracted and analyzed by Western immunoblot utilizing specific rabbit polyclonal anti-Pin l I (a homologue of Pin m III) antibodies. During all collection dates, but particularly in the spring, susceptible trees had more Pin m III than resistant trees. In July 1995, 43 previously inoculated 7-year-old white pine seedlings were also analyzed. In all susceptible seedlings (cankered) tested, cankered tissue had high levels of Pin m III, and samples collected from the outside edge of the canker margin had low levels of Pin m III; this protein was also detected in some healthy bark of cankered trees. Since the level of Pin m III in healthy white pine trees is normally lowest in summer months, the high level Pin m III in summer samples of infected tissues is a consequence of the fungal infection.

Protection of Disease-Resistant Western White Pine Seed from Insect Damage

1988 ◽  
Vol 3 (1) ◽  
pp. 18-20 ◽  
Author(s):  
Michael I. Haverty ◽  
Patrick J. Shea ◽  
Lawrence E. Stipe
Keyword(s):  
Seed Orchard ◽  
Insect Damage ◽  
Pinus Monticola ◽  
Botanical Insecticide ◽  
White Pine ◽  
Dioryctria Abietivorella ◽  
Western White Pine ◽  
Insect Populations ◽  
Repeated Applications ◽  
Seed Crops

Abstract Seed produced in blister rust-resistant western white pine (Pinus monticola Douglas) seed orchards is in high demand. The fir coneworm, Dioryctria abietivorella (Groté), and the lodgepole pine coneworm, Eucosma recissoriana (Heinrich), cause considerable damage to seed crops in the Moscow Arboretum seed orchard. Single (May or June) and repeated (May and June) applications of 0.025% fenvalerate, and repeated applications (May and June) of 0.0125% fenvalerate, a synthetic botanical insecticide, were evaluated in 1986 for protection of cone crops. Results in 1986 confirm results in 1984 (Haverty et al. 1986): an application of 0.025% fenvalerate in May and June significantly reduces insect damage, regardless of the relative abundance of cones or insects. The decision to use a single or repeated application depends on demand for seed, projected seed crop and insect populations, and socioeconomic costs of the treatment. West. J. Appl. For. 3(1):18-20, January 1988.

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