Improved AFLP analysis of tree species

2000 ◽  
Vol 30 (10) ◽  
pp. 1608-1616 ◽  
Author(s):  
M T Cervera ◽  
D Remington ◽  
J -M Frigerio ◽  
V Storme ◽  
B Ivens ◽  
...  
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Tree Species ◽  
Prunus Persica ◽  
Genome Mapping ◽  
Woody Species ◽  
Forest Tree ◽  
Aflp Analysis ◽  
Tree Genetics ◽  
Pinus Taeda L

Amplified fragment length polymorphism (AFLP) is a high-throughput, molecular-marker technique that is used increasingly in a variety of genetic analyses. Here, the conditions for carrying out AFLP analysis have been established for different tree species, including both angiosperm and gymnosperm trees, with genome sizes ranging from 0.54 to 38 pg DNA/2C. Specific parameters have been determined to provide informative and reproducible AFLP fingerprints of peach (Prunus persica L.), eucalypt, oak, poplar, and loblolly pine (Pinus taeda L.). Typically, 80-130 amplified DNA fragments (i.e., loci analyzed per primer combination) were obtained. Subsequently, these AFLP conditions were evaluated for intra- and inter-specific genetic variability studies as well as for genome mapping purposes of woody species. This work demonstrates that AFLP is a powerful tool in forest tree genetics.

scholarly journals The Association of a Longidorus Species with Stunting and Root Damage of Loblolly Pine (Pinus taeda L.) Seedlings

Plant Disease ◽  
2002 ◽  
Vol 86 (7) ◽  
pp. 803-807 ◽  
Author(s):  
Stephen W. Fraedrich ◽  
Michelle M. Cram
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Dry Weight ◽  
Forest Tree ◽  
Root Weight ◽  
Extraction Techniques ◽  
Seedling Root ◽  
Pine Seedlings ◽  
Proper Diagnosis ◽  
Pinus Taeda L

A Longidorus species was consistently associated with patches of stunted and chlorotic loblolly pine seedlings at a forest-tree nursery in Georgia. Seedlings from affected areas had poorly developed root systems that lacked lateral and feeder roots. Longidorus population densities in composite soil samples from the margins of patches ranged from 9 to 67 nematodes per 100 cm3 of soil. In a growth chamber experiment, seedling root dry weight decreased with respect to the initial Longidorus dose as well as the final Longidorus populations in containers. The dry root weight of seedlings were 0.117, 0.090, 0.066, and 0.065 g in containers initially infested with 0, 50, 100, and 200 Longidorus, respectively. Lateral and fine roots were lacking on seedlings at the highest doses. Populations of Longidorus increased in all containers during the experiment. Damage to loblolly pine seedlings caused by Longidorus is a previously undescribed problem in southern pine nurseries. Proper diagnosis of the problem by nematode testing laboratories may require the use of extraction techniques specific for larger nematodes such as Longidorus.

scholarly journals Controlled mass pollination in loblolly pine to increase genetic gains

1998 ◽  
Vol 74 (2) ◽  
pp. 185-189 ◽  
Author(s):  
F. E. Bridgwater ◽  
D. L. Bramlett ◽  
T. D. Byram ◽  
W. J. Lowe
Keyword(s):  
Key Words ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Forest Tree ◽  
Pilot Scale ◽  
Tree Improvement ◽  
Improvement Program ◽  
Seed Orchards ◽  
Genetic Gains ◽  
Pinus Taeda L

Controlled mass pollination (CMP) is one way to increase genetic gains from traditional wind-pollinated seed orchards. Methodology is under development by several forestry companies in the southern USA. Costs of CMP depend on the efficient installation, pollination, and removal of inexpensive paper bags. Even in pilot-scale studies these costs seem reasonable. Net present values from CMP in a sample of sixty-seven loblolly pine (Pinus taeda L.) seed orchards in the Western Gulf Forest Tree Improvement Program are conservatively expected to average $108 per acre of plantation established with seedlings from CMP among the best six parents in each of five breeding regions and $154 per acre for CMP among the best pair of parents in each breeding region. Key words: supplemental mass pollination, expected genetic gains, pollen contamination

Correspondence between Breeding Values of the Same Pinus taeda L. Genotypes from Clonal Trials and Half-Sib Seedling Progeny Trials

2020 ◽  
Vol 66 (5) ◽  
pp. 600-611
Author(s):  
Mohammad Nasir Shalizi ◽  
Salvador Alejandro Gezan ◽  
Steven E McKeand ◽  
Joshua R Sherrill ◽  
W Patrick Cumbie ◽  
...  
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Additive Genetic Variance ◽  
Tree Height ◽  
Forest Tree ◽  
Stem Volume ◽  
Breeding Values ◽  
Progeny Tests ◽  
Pinus Taeda L ◽  
Analytical Approaches

Abstract The correspondence between breeding values of 65 loblolly pine (Pinus taeda L.) genotypes from clonal genetic tests and half-sib seedling progeny tests was studied in the southern United States. The two experiments were established separately, 10 years apart. Additive genetic variance estimates from clonal tests were larger compared with the estimates from the half-sib progeny tests, regardless of the covariance structure used in the statistical models and the traits. However, clone-mean and half-sib family-mean heritability estimates were comparable for all traits, ranging between 0.88 and 0.99. Based on the independent analysis, the correlation between the breeding values of the same genotypes from two propagule types was moderate (0.59) for tree height and stem volume. The combined analysis resulted in a strong genetic correlation (>0.93) between the breeding values of two propagule types. Herein the large discrepancy is mainly the outcome of different data analytical approaches. Conclusively, selecting genotypes for deployment based on clonal testing may not be optimal, but forest tree breeders can use the results from clonal tests to make some informed decisions.

scholarly journals Fumigation with Chloropicrin, Metham Sodium, and EPTC as Replacements for Methyl Bromide in Southern Pine Nurseries

2000 ◽  
Vol 24 (3) ◽  
pp. 135-139 ◽  
Author(s):  
William A. Carey
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Unit Area ◽  
Forest Tree ◽  
Soil Fumigation ◽  
Average Potential ◽  
Collar Diameter ◽  
Pinus Taeda L ◽  
Root Collar ◽  
Metham Sodium

Abstract The effects of soil fumigation with chloropicrin alone or in combination with metham sodium and or with EPTC on the growth of loblolly pine (Pinus taeda L.) seedlings and on nutsedge (Cyperus spp.) control were investigated at three forest tree nurseries. Fumigation with chloropicrin plus metham sodium (CMS) significantly increased seedling numbers and growth compared to controls. Averaged among nurseries, mean root-collar-diameter increased from 0.14 to 0.17 in., and biomass from 0.14 to 0.20 lb/ft2. This produced seven more plantable seedlings and 6.5 more grade 1 seedlings/ft2 in CMS compared to not fumigated plots. Fumigation increased average potential sales $7,100/ac and increased present values, projected for the growth increases for additional grade 1 seedlings, by $25,900/ac. Fumigation increased biomass per unit area similarly at tested bed densities (10 to 29/ft2), but at low bed densities (≤20/ft2) the present values associated with grade 1 seedlings increased more than at higher densities. South. J. Appl. For. 24(3):135-139.

scholarly journals A Consensus Map for Loblolly Pine (Pinus taeda L.). I. Construction and Integration of Individual Linkage Maps From Two Outbred Three-Generation Pedigrees

Genetics ◽  
1999 ◽  
Vol 151 (1) ◽  
pp. 321-330 ◽  
Author(s):  
Mitchell M Sewell ◽  
Bradley K Sherman ◽  
David B Neale
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Mapping Function ◽  
Genetic Maps ◽  
Linkage Data ◽  
Linkage Maps ◽  
Consensus Map ◽  
Pinus Taeda L

Abstract A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree were recoded to reflect the two independent populations of parental meioses, and genetic maps were constructed to represent each parent. The rate of meiotic recombination was significantly greater for males than females, as was the average estimate of genome length for males {1983.7 cM [Kosambi mapping function (K)]} and females [1339.5 cM(K)]. The integration of individual maps allows for the synthesis of genetic information from independent sources onto a single consensus map and facilitates the consolidation of linkage groups to represent the chromosomes (n = 12) of loblolly pine. The resulting consensus map consists of 357 unique molecular markers and covers ∼1300 cM(K).

Long-term effects of stand density management and genotype on wood properties of loblolly pine (Pinus taeda L.) in the mid-South USA

2021 ◽  
Vol 491 ◽  
pp. 119176
Author(s):  
Michael A. Blazier ◽  
Thomas Hennessey ◽  
Laurence Schimleck ◽  
Scott Abbey ◽  
Ryan Holbrook ◽  
...  
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Stand Density ◽  
Wood Properties ◽  
Long Term Effects ◽  
Stand Density Management ◽  
Pinus Taeda L

scholarly journals Four Site-Preparation Techniques for Regenerating Pine-Hardwood Mixtures in the Piedmont

1997 ◽  
Vol 21 (3) ◽  
pp. 116-122 ◽  
Author(s):  
Thomas A. Waldrop
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Height Growth ◽  
Site Preparation ◽  
Site Conditions ◽  
Growing Seasons ◽  
Southern Appalachian ◽  
Medium Quality ◽  
Pinus Taeda L ◽  
Preparation Techniques

Abstract Four variations of the fell-and-burn technique, a system developed to produce mixed pine-hardwood stands in the Southern Appalachian Mountains, were compared in the Piedmont region. All variations of this technique successfully improved the commercial value of low-quality hardwood stands by introducing a pine component. After six growing seasons, loblolly pine (Pinus taeda L.) occupied the dominant crown position and oaks the codominant position in fell-and-burn treated stands on poor to medium quality sites. The precise timing of felling residual stems, as prescribed by the fell-and-burn technique, may be flexible because winter and spring felling produced similar results. Although summer site preparation burns reduced hardwood height growth by reducing the length of the first growing season, they did not improve pine survival or growth. Pines were as tall as hardwoods within four growing seasons in burned plots and within six growing seasons in unburned plots. Additional research is needed to determine the level or intensity of site preparation needed to establish pine-hardwood mixtures over a range of site conditions. South. J. Appl. For. 21(3):116-122.

scholarly journals Genotypic Stability Effects on Predicted Family Responses to Silvicultural Treatments in Loblolly Pine

1997 ◽  
Vol 21 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Steven E. McKeand ◽  
Robert P. Crook ◽  
H. Lee Allen
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Growth Responses ◽  
Intensive Culture ◽  
Genotype By Environment ◽  
Silvicultural Treatments ◽  
Genotypic Stability ◽  
Superior Genotypes ◽  
Using Data ◽  
Pinus Taeda L

Abstract The lack of rank change in growth characteristics when open-pollinated families of loblolly pine (Pinus taeda L.) are planted on different sites in the Southeast has greatly simplified breeding for superior genotypes. Although family rank does not usually change, genotype by environment interactions (GxE) may be very important in operational deployment of families in regeneration programs. Using data from GxE trials and two site preparation-fertilization-herbicide trials, we estimated the growth that different families should achieve following application of these silvicultural practices. Better performing families tend to be most responsive to site changes (i.e. genetically unstable). Growth responses to silvicultural treatment will be overestimated if only the most responsive families are used in silvicultural research trials. Similarly, genetic gains will be overestimated if gain trials are planted on only the best sites or receive intensive culture. South. J. Appl. For. 21(2):84-89.

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