Application of the GUS marker gene technique to high-throughput screening of rhizobial competition

1999 ◽  
Vol 45 (8) ◽  
pp. 678-685 ◽  
Author(s):  
Kate J Wilson ◽  
Adriana Parra ◽  
Lina Botero
Keyword(s):  
Phaseolus Vulgaris ◽  
Sample Size ◽  
Microbial Ecology ◽  
High Throughput ◽  
High Throughput Screening ◽  
Marker Gene ◽  
Nodule Occupancy ◽  
Gus Gene ◽  
Gus Assay ◽  
Rhizobium Strains

The GUS marker gene system has been developed for the study of bacterial ecology, particularly rhizobial competition. For high-throughput field screening of rhizobial competition, the technique must be robust and reliable under diverse conditions, with diverse cultivars and strains. Here we demonstrate its applicability to the evaluation of competition on five different Phaseolus vulgaris cultivars with 10 different Rhizobium strains. We describe refinements of the GUS assay, which make it more affordable and applicable to field-based studies, and use the assay to examine the effect of sample size on the accuracy of nodule occupancy measurements.Key words: GUS gene, Rhizobium, rhizobial competition, microbial ecology.

scholarly journals Freeze-Float System for High-throughput Measurement of Ice Nucleation

2021 ◽  
Author(s):  
Yuki Kamijo ◽  
Ratmir Derda
Keyword(s):  
Temperature Distribution ◽  
Sample Size ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cooling System ◽  
Ice Nucleation ◽  
Uniform Temperature ◽  
Detection Program ◽  
Accuracy Of Measurement ◽  
Platform System

In this publication, we developed the high throughput screening implementation of freeze-float selection platform system we established in the previous publication. The goal of this publication is to expand the system to higher throughput and accuracy. In the following sections, we describe the steps for automated droplets generations, adaptation of freeze-float selection using controlled and uniform temperature cooling system, and semi-automated droplets detection program. We aimed to improve previously published system to add functional advantages, such as; a) increased efficiency of the screening with fewer manipulation steps; b) increased accuracy of measurement due to the increased sample size; c) increased uniformity of temperature distribution by incorporating the controlled-rate freezer. In the following sections, we describe the steps for automated droplets generations, freeze-float system adaptations using controlled and uniform temperature cooling system, and semi-automated droplets detection program.

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome

High-throughput screening revealed a clinically relevant drug to induce sperm motility

2014 ◽  
Author(s):  
Clair Cochrane ◽  
Halil Ruso ◽  
Anthony Hope ◽  
Rosemary G Clarke ◽  
Christopher Barratt ◽  
...  
Keyword(s):  
Sperm Motility ◽  
High Throughput ◽  
High Throughput Screening
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