Salmonella landau as a live vaccine against Escherichia coli O157:H7 investigated in a mouse model of intestinal colonization

1999 ◽  
Vol 45 (9) ◽  
pp. 723-731 ◽  
Author(s):  
J Wayne Conlan ◽  
Rhonda KuoLee ◽  
Ann Webb ◽  
Malcolm B Perry
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Mouse Model ◽  
Mucosal Immunity ◽  
Host Resistance ◽  
Mucosal Immune Response ◽  
Escherichia Coli O157 ◽  
Local Immune Response ◽  
Intestinal Colonization ◽  
E Coli

The present study was performed to assess the potential of a humoral mucosal immune response directed against the O157 antigen of Escherichia coli O157:H7 to prevent intestinal colonization by the pathogen. To this end, mice were gavaged with inocula of Salmonella landau, a Salmonella strain that naturally expresses the O157 antigen. Salmonella landau was avirulent for mice. Despite this, mice exposed to S. landau developed high titres of serum and coproantibodies against the O157 antigen. These mice, compared with controls, demonstrated some ability to resist transient intestinal colonization by an oral inoculum of an isolate of E. coli O157:H7. These findings suggest that a local immune response directed against the O157 antigen might increase host resistance to this pathogen.Key words: Salmonella landau, Escherichia coli O157:H7, mucosal immunity, mice.

Ability ofEscherichia coliO157:H7 isolates to colonize the intestinal tract of conventional adult CD1 mice is transient

2001 ◽  
Vol 47 (1) ◽  
pp. 91-95 ◽  
Author(s):  
J Wayne Conlan ◽  
Sonia L Bardy ◽  
Rhonda KuoLee ◽  
Ann Webb ◽  
Malcolm B Perry
Keyword(s):  
Escherichia Coli ◽  
Mouse Model ◽  
Intestinal Tract ◽  
Vaccine Development ◽  
Escherichia Coli O157 ◽  
Intestinal Colonization ◽  
E Coli ◽  
Cd1 Mice ◽  
A Current

In an attempt to improve upon a current mouse model of intestinal colonization by Escherichia coli O157:H7 used in this laboratory for vaccine development, nine clinical isolates of the pathogen were screened for their ability to persist in the intestinal tract of conventional adult CD-1 mice. None of the test isolates of E. coli O157:H7 were capable of colonizing these mice for a period of more than two weeks. Most of the isolates appeared to be benign for the experimental host, but one isolate was lethal. This virulence correlated with the ability of the latter isolate to produce large quantities of Shiga-like toxin 2 in vitro.

Parenteral immunization with a glycoconjugate vaccine containing the O157 antigen of Escherichia coli O157:H7 elicits a systemic humoral immune response in mice, but fails to prevent colonization by the pathogen

1999 ◽  
Vol 45 (4) ◽  
pp. 279-286
Author(s):  
J Wayne Conlan ◽  
Andrew D Cox ◽  
Rhonda KuoLee ◽  
Ann Webb ◽  
Malcolm B Perry
Keyword(s):  
Escherichia Coli ◽  
Intestinal Tract ◽  
Horse Serum ◽  
Convincing Evidence ◽  
Escherichia Coli O157 ◽  
Intestinal Colonization ◽  
E Coli ◽  
Humoral Immune ◽  
Parenteral Immunization ◽  
Glycoconjugate Vaccine

The results of the present study show that whereas both BALB/c and C57BL/6 mice parenterally inoculated with a horse serum albumin - Escherichia coli O157 antigen conjugate vaccine develop systemic, specific antibodies to the carrier protein, only the former mice routinely develop antibodies to the carbohydrate O157 moiety. However, little convincing evidence was found to show that these antibodies transuded into the intestinal tract either naturally or in response to an oral inoculum of the pathogen. Moreover, this vaccination procedure failed to protect mice against intestinal colonization following oral challenge with the pathogen. Thus, the results of this study suggest that parenteral vaccination might be an unsuitable strategy for combatting E. coli O157:H7 organisms located in the gut.Key words: Escherichia coli, glycoconjugate vaccine, mice.

Oral immunization of mice with a glycoconjugate vaccine containing the O157 antigen of Escherichia coli O157:H7 admixed with cholera toxin fails to elicit protection against subsequent colonization by the pathogen

2000 ◽  
Vol 46 (3) ◽  
pp. 283-290 ◽  
Author(s):  
J Wayne Conlan ◽  
Rhonda KuoLee ◽  
Ann Webb ◽  
Andrew D Cox ◽  
Malcolm B Perry
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Cholera Toxin ◽  
Horse Serum ◽  
Oral Immunization ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Humoral Immune ◽  
Unvaccinated Control ◽  
Glycoconjugate Vaccine

It has been postulated that a humoral immune response directed against the O157 antigen of Escherichia coli O157:H7, and expressed in the intestine, might afford protection from colonization and consequent infection by this enteric pathogen. The present study was conducted to determine whether such an immune response can be experimentally generated in mice. To this end, mice were orally immunized with a glycoconjugate vaccine consisting of horse serum albumin and the O157 polysaccharide admixed with the mucosal adjuvant, cholera toxin. Mice consistently developed robust local and systemic immune responses to the cholera toxin adjuvant, but were far from uniformly reactive to the test vaccine. Moreover, vaccinated mice were as susceptible to transient intestinal colonization following challenge with an isolate of E. coli O157:H7 as unvaccinated control mice. These results indicate that this vaccination approach is unlikely to be straightforward in target bovine or human hosts.Key words: Escherichia coli O157:H7, glycoconjugate vaccine, mucosal immunity, mice.

scholarly journals Long Polar Fimbriae Contribute to Colonization by Escherichia coli O157:H7 In Vivo

2004 ◽  
Vol 72 (10) ◽  
pp. 6168-6171 ◽  
Author(s):  
Dianna M. Jordan ◽  
Nancy Cornick ◽  
Alfredo G. Torres ◽  
Evelyn A. Dean-Nystrom ◽  
James B. Kaper ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Parent Strain ◽  
Escherichia Coli O157 ◽  
Intestinal Colonization ◽  
E Coli ◽  
Gnotobiotic Piglets

ABSTRACT The contribution of long polar fimbriae to intestinal colonization by Escherichia coli O157:H7 was evaluated in sheep, conventional pigs, and gnotobiotic piglets. E. coli O157:H7 strains with lpfA1 and lpfA2 mutated were recovered in significantly lower numbers and caused fewer attachment and effacement lesions than the parent strain.

scholarly journals Escherichia coli O157:H7 and Other E. coli Strains Share Physiological Properties Associated with Intestinal Colonization

2009 ◽  
Vol 75 (13) ◽  
pp. 4633-4635 ◽  
Author(s):  
Lisa Jacobsen ◽  
Lisa Durso ◽  
Tyrell Conway ◽  
Kenneth W. Nickerson
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Growth Parameters ◽  
Escherichia Coli O157 ◽  
Intestinal Colonization ◽  
E Coli ◽  
Physiological Properties ◽  
Gastrointestinal Colonization

ABSTRACT Escherichia coli isolates (72 commensal and 10 O157:H7 isolates) were compared with regard to physiological and growth parameters related to their ability to survive and persist in the gastrointestinal tract and found to be similar. We propose that nonhuman hosts in E. coli O157:H7 strains function similarly to other E. coli strains in regard to attributes relevant to gastrointestinal colonization.

Susceptibility of three strains of conventional adult mice to intestinal colonization by an isolate of Escherichia coli O157:H7

1998 ◽  
Vol 44 (8) ◽  
pp. 800-805 ◽  
Author(s):  
J Wayne Conlan ◽  
Malcolm B Perry
Keyword(s):  
Escherichia Coli ◽  
Vaccine Development ◽  
Immunoglobulin A ◽  
Bacterial Pathogen ◽  
Mouse Strains ◽  
Escherichia Coli O157 ◽  
Intestinal Colonization ◽  
E Coli ◽  
Adult Mice ◽  
Enhanced Resistance

Three mouse strains were assessed for their susceptibility to intestinal colonization by a strain of the enteric bacterial pathogen Escherichia coli O157:H7. Following intragastric inoculation of E. coli O157:H7, the intestines of young adult female CD1, BALB/c, and C57BL/6 mice became colonized, as evidenced by faecal shedding of the pathogen for periods of up to 5 weeks. None of the three mouse strains examined developed overt disease in response to colonization by the organism. Following clearance of the primary inoculum, BALB/c mice, but not CD1 or C57BL/6 mice, appeared to acquire enhanced resistance to recolonization by E. coli O157:H7, as evidenced by a decreased faecal shedding period. This enhanced resistance correlated with the presence and persistence of immunoglobulin A, but not immunoglobulin G, in the serum and faeces directed against the O157 antigen. The implications of these findings to vaccine development against E. coli O157:H7 are discussed.Key words: E. coli O157:H7, CD1 mice, BALB/c mice, C57BL/6 mice, faecal shedding.

Antimicrobial Effects of Zataria multiflora and Ocimum basilicum on Escherichia coli O157:H7 During Ripening of Traditional Lighvan Cheese

2020 ◽  
Vol 16 (3) ◽  
pp. 373-380
Author(s):  
Mohammad B. Zendeh ◽  
Vadood Razavilar ◽  
Hamid Mirzaei ◽  
Khosrow Mohammadi
Keyword(s):  
Escherichia Coli ◽  
Essential Oils ◽  
Dairy Products ◽  
Antimicrobial Agents ◽  
Escherichia Coli O157 ◽  
Zataria Multiflora ◽  
Antimicrobial Effects ◽  
E Coli ◽  
Common Causes ◽  
Lighvan Cheese

Background: Escherichia coli O157:H7 is one of the most common causes of contamination in Lighvan cheese processing. Using from natural antimicrobial essential oils is applied method to decrease the rate of microbial contamination of dairy products. The present investigation was done to study the antimicrobial effects of Z. multiflora and O. basilicum essential oils on survival of E. coli O157:H7 during ripening of traditional Lighvan cheese. Methods: Leaves of the Z. multiflora and O. basilicum plants were subjected to the Clevenger apparatus. Concentrations of 0, 100 and 200 ppm of the Z. multiflora and 0, 50 and 100 ppm of O. basilicum essential oils and also 103 and 105 cfu/ml numbers of E. coli O157:H7 were used. The numbers of the E. coli O157:H7 bacteria were analyzed during the days 0, 30, 60 and 90 of the ripening period. Results: Z. multiflora and O. basilicum essential oils had considerable antimicrobial effects against E. coli O157:H7. Using the essential oils caused decrease in the numbers of E. coli O157:H7 bacteria in 90th days of ripening (P <0.05). Using from Z. multiflora at concentration of 200 ppm can reduce the survival of E. coli O157:H7 in Lighvan cheese. Conclusion: Using Z. multiflora and O. basilicum essential oils as good antimicrobial agents can reduce the risk of foodborne bacteria and especially E. coli O157:H7 in food products.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Bacteriophages reduce Escherichia coli O157:H7 levels in experimentally inoculated sheep

2009 ◽  
Vol 89 (2) ◽  
pp. 285-293 ◽  
Author(s):  
S J Bach ◽  
R P Johnson ◽  
K. Stanford ◽  
T A McAllister
Keyword(s):  
Escherichia Coli ◽  
Oral Administration ◽  
Coliform Bacteria ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Total Coliforms ◽  
E Coli ◽  
Oral Inoculation ◽  
Experimental Treatments ◽  
And Control

Bacteriophage biocontrol has potential as a means of mitigating the prevalence of Escherichia coli O157:H7 in ruminants. The efficacy of oral administration of bacteriophages for reducing fecal shedding of E. coli O157:H7 by sheep was evaluated using 20 Canadian Arcott rams (50.0 ± 3.0) housed in four rooms (n = 5) in a contained facility. The rams had ad libitum access to drinking water and a pelleted barley-based total mixed ration, delivered once daily. Experimental treatments consisted of administration of E. coli O157:H7 (O157), E. coli O157:H7+bacteriophages (O157+phage), bacteriophages (phage), and control (CON). Oral inoculation of the rams with 109 CFU of a mixture of four nalidixic acid-resistant strains of E. coli O157:H7 was performed on day 0. A mixture of 1010 PFU of bacteriophages P5, P8 and P11 was administered on days -2, -1, 0, 6 and 7. Fecal samples collected on 14 occasions over 21 d were analyzed for E. coli O157:H7, total E. coli, total coliforms and bacteriophages. Sheep in treatment O157+phage shed fewer (P < 0.05) E. coli O157:H7 than did sheep in treatment O157. Populations of total coliforms and total E. coli were similar (P < 0.05) among treatments, implying that bacteriophage lysis of non-target E. coli and coliform bacteria in the gastrointestinal tract did not occur. Bacteriophage numbers declined rapidly over 21 d, which likely reduced the chance of collision between bacteria and bacteriophage. Oral administration of bacteriophages reduced shedding of E. coli O157:H7 by sheep, but a delivery system that would protect bacteriophages during passage through the intestine may increase the effectiveness of this strategy as well as allow phage to be administered in the feed.Key words: Escherichia coli O157:H7, bacteriophage, sheep, environment, coliforms

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