Esterase activity and release of ethyl esters of medium-chain fatty acids by Saccharomyces cerevisiae during anaerobic growth

1998 ◽  
Vol 44 (12) ◽  
pp. 1171-1176 ◽  
Author(s):  
Laura Bardi ◽  
Cristina Crivelli ◽  
Mario Marzona
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Ethyl Ester ◽  
Anaerobic Fermentation ◽  
Ethyl Esters ◽  
Anaerobic Growth ◽  
Ester Synthesis ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Chain Fatty Acids

During anaerobic fermentation, Saccharomyces cerevisiae releases large amounts of medium-chain fatty acids (MCFAs) and related ethyl esters which are very important for aromatic quality of fermented beverages. The physiological function of ester synthesis is not yet understood. As MCFAs are toxic, their conversion to esters has been proposed to be a detoxification mechanism. Esterases possess ester synthesizing ability. Throughout an anaerobic fermentation of a lipid-free synthetic medium carried out with a S. cerevisiae strain selected for wine making, we have monitored MCFA and ethyl ester production and, at the same time, measured growth and esterasic activity of intact cells. Because no correlation was found between the concentration of each fatty acid and its ethyl ester, there is no evidence that ester synthesis reduces the toxicity of MCFAs. Esterasic activity did not show any correlation with ester synthesis, but it was related to the release of MCFAs. A model is proposed in which ester synthesis is a consequence of the arrest of lipid biosynthesis resulting from a lack of oxygen. Under these conditions, an excess of acyl coenzyme A is produced, and acyl esters are formed as secondary products of reactions aimed at recovering free coenzyme A.Key words: yeast, esterase, medium-chain fatty acids, toxicity, ethyl esters.

Multidimensional engineering of Saccharomyces cerevisiae for efficient synthesis of medium-chain fatty acids

2020 ◽  
Vol 3 (1) ◽  
pp. 64-74 ◽  
Author(s):  
Zhiwei Zhu ◽  
Yating Hu ◽  
Paulo Gonçalves Teixeira ◽  
Rui Pereira ◽  
Yun Chen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Efficient Synthesis ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Chain Fatty Acids

scholarly journals EFFICACY OF MEDIUM CHAIN FATTY ACIDS AND SACCHAROMYCES CEREVISIAE ON GROWTH PERFORMANCE OF BROILER

2018 ◽  
Vol 16 (1) ◽  
pp. 31-38
Author(s):  
M. S. Islam ◽  
M. S. Rahman ◽  
M. A. Islam ◽  
S. M. S. H. Belal
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Treatment Group ◽  
Feed Consumption ◽  
Control Group ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Significant Difference ◽  
Group 2 ◽  
Chain Fatty Acids

The study was conducted to determine the effect of medium chain fatty acids and Saccharomyces cerevisiae on performance in broiler birds. A field trial was carried out on 200 broiler chicks ((Lohman) dividing them into four treatment groups each having 50 birds such as treatment group 1 (T1), treatment group 2 (T2), treatment group 3 (T3), and a control group (C). The treatment group 1 (T1) was supplied with a standard feed containing medium chain fatty acids (MCFA) and Saccharomyces cerevisiae (S. cerevisiae type boulardii 2.0x1010 CFU/gm).Treatment group 2 (T2) was supplied with a standard feed rich in medium chain fatty acids (MCFA). Treatment group 3 (T3) was supplied with a standard feed having Saccharomyces cerevisiae (S. cerevisiae type boulardii 2.0x1010 CFU/gm. MCFA and S. cerevisiae lacked standard feed was given to the control group(C).  The inclusion rate of MCFA per kilogram of feed was 2 gm for 10 days, 1.5 gm for successive 10 days and 1gm for next 8 days, and of Saccharomyces cerevisiae was 0.2gm for 10 days, 0.15 gm for successive 10 days and 0.10 gm for next 8 days during the study period of 4 weeks. Weekly weight gain and, at the end of the study, body weight, feed consumption, FCR and mortality rate were recorded. Analysis of data revealed that the final average body weight and average weight gain of the birds belonging to the groups T1, T2 and T3 were higher (p< 0.01) than the control group. FCR was 1.36, 1.37, and 1.38 for the treatment group T1, T3 and T2 respectively which were better than (p<0.05) control group. There was no significant difference between the treatment group T2 and T3 but there was significant difference between the treatment group and control group (p<0.10) in terms of feed consumption. In case of livability, there was significant difference among bird groups T1, T2, T3 and control group (p<0.01). Data analyses regarding organ weight showed that the addition of MCFA and SC in the diet of treatment group one (T1) significantly increased (p<0.01) the weight of intestine with chymus, intestine without chymus, gizzard, head and neck by 41%, 17%, 5%, 8%  and 11% respectively compared with  control group. No significant differences were observed among the experimental groups for the weight of liver, spleen, and pancreas.

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