Effect of aromatic compounds on growth and ligninolytic enzyme production of two white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus

1998 ◽  
Vol 44 (9) ◽  
pp. 872-885 ◽  
Author(s):  
Anand Sethuraman ◽  
Danny E Akin ◽  
Jason G Eisele ◽  
Karl-Erik L Eriksson
Keyword(s):  
Cinnamic Acid ◽  
Aromatic Compounds ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Hyphal Growth ◽  
White Rot ◽  
Ceriporiopsis Subvermispora ◽  
Cyathus Stercoreus ◽  
Benzaldehyde Derivatives

Seven benzoic acid, ten cinnamic acid, and five benzaldehyde derivatives were tested for their effects on hyphal growth and production of laccase and manganese peroxidase by Ceriporiopsis subvermispora FP 90031-sp and Cyathus stercoreus ATCC 36910. Derivatives tested included phenolic compounds and their corresponding unsubstituted and O-methylated derivatives. Benzaldehyde derivatives were more toxic to both fungi than the corresponding benzoic and cinnamic acid derivatives. Hyphal growth was generally increased at a low concentration of 1 mM, while higher concentrations of 5-10 mM mostly resulted in less or no growth. Hyphal growth and enzyme production response were compound specific. However, generally monomethoxylated compounds were more toxic than compounds with an additional methoxyl group. Cyathus stercoreus was more sensitive than Ceriporiopsis subvermispora to most of the compounds tested and thus showed poorer growth. Cyathus stercoreus produced higher concentrations of manganese peroxidase than Ceriporiopsis subvermispora for all the compounds tested, whereas laccase activity was higher in Ceriporiopsis subvermispora for most of the compounds tested. Di- and tri-methoxylated compounds induced more laccase and manganese peroxidase activities than the corresponding hydroxylated derivatives. At 1 mM levels, 3,4-dimethoxycinnamic acid induced the greatest increase in laccase production for Ceriporiopsis subvermispora and Cyathus stercoreus (245 and 290% of control, respectively). Syringic acid induced manganese peroxidase (MnP) to 536% of that in control for Ceriporiopsis subvermispora, and both 3,4-dimethoxycinnamic acid and 3,4,5-trimethoxycinnamic acid induced MnP to over 300% of control for Cyathus stercoreus. The results provide a body of information on the effects of specific aromatic compounds on two potentially industrially important fungi. Key words: biomass conversion, aromatic compounds, white rot fungi, fungal growth, enzyme production.

scholarly journals Laccase and Manganese Peroxidase (MnP) Activities in the White-Rot Fungus Trametes hirsuta in Response to Aromatic Compounds

2020 ◽  
Vol 23 (2) ◽  
pp. 66
Author(s):  
Isa Nuryana ◽  
Zidny Ilmiah ◽  
Ade Andriani ◽  
Yopi Yopi
Keyword(s):  
Gallic Acid ◽  
Caffeic Acid ◽  
Cinnamic Acid ◽  
Aromatic Compounds ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
Veratryl Alcohol ◽  
Syringic Acid ◽  
White Rot ◽  
Trametes Hirsuta

The current interest in exploring white-rot fungi has been concentrated in increasing their ligninolityc enzyme production such as laccase and manganese peroxidase (MnP) due to the great value in industrial application. The presence of appropiate inducers can enhance the enzyme production. Hence, the aim of the study was to investigate the activity of  laccase and MnP in response to various aromatic compounds. The fungus Trametes hirsuta was cultured in Glucose Yeast Peptone (GYP) broth (pH 5.0) with the addition of 0.5% of sorghum biomass as substrate. The cultures were then incubated on rotary shaker at 150 rpm at 27oC. The 7-day-old cultures were then supplemented with different aromatic compounds, namely caffeic acid, gallic acid, syringic acid, trans-cinnamic acid, vanillin, and veratryl alcohol. Our results demonstrated that veratryl alcohol, gallic acid and vanillin gave an inductive effect on the value of laccase and MnP activities, with vanillin showing the highest induction. On the contrary, the level of laccase and MnP activities remained low in the presence of aromatic compound such as syringic acid, trans-cinnamic acid and caffeic acid. To conclude, our study reveals that aromatic compounds can be potential as inducers and may contribute to the improvement of laccase and MnP productivity by T. hirsuta.

Biological delignification of plant components by the white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus

1996 ◽  
Vol 63 (1-4) ◽  
pp. 305-321 ◽  
Author(s):  
D.E. Akin ◽  
W.H. Morrison ◽  
L.L. Rigsby ◽  
G.R. Gamble ◽  
A. Sethuraman ◽  
...  
Keyword(s):  
White Rot Fungi ◽  
White Rot ◽  
Ceriporiopsis Subvermispora ◽  
Cyathus Stercoreus ◽  
Plant Components

scholarly journals Bioremediation of Direct Blue 14 and Extracellular Ligninolytic Enzyme Production by White Rot Fungi:PleurotusSpp.

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
M. P. Singh ◽  
S. K. Vishwakarma ◽  
A. K. Srivastava
Keyword(s):  
Enzymatic Activity ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Dye Decolorization ◽  
Ligninolytic Enzyme ◽  
Enzymatic Activities ◽  
White Rot ◽  
Direct Blue

In the present investigation, four species of white rot fungi (Pleurotus), that is,P. flabellatus, P. florida, P. ostreatusandP. sajor-cajuwere used for decolorization of direct blue 14 (DB14). Among all four species ofPleurotus,P. flabellatusshowed the fastest decolorization in petri plates on different concentration, that is, 200 mg/L, 400 mg/L, and 600 mg/L. All these four species were also evaluated for extracellular ligninolytic enzymes (laccase and manganese peroxidase) production and it was observed that the twelve days old culture ofP. flabellatusshowed the maximum enzymatic activity, that is, 915.7 U/mL and 769.2 U/mL of laccase and manganese peroxidase, respectively. Other threePleurotusspecies took more time for dye decolorization and exhibited less enzymatic activities. The rate of decolorization of DB14 dye solution (20 mg/L) by crude enzymes isolated fromP. flabellatuswas very fast, and it was observed that up to 90.39% dye solution was decolorized in 6 hrs of incubation.

Effect of growth conditions on the production of manganese peroxidase by three strains of Bjerkandera adusta

2001 ◽  
Vol 47 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Yuxin Wang ◽  
Rafael Vazquez-Duhalt ◽  
Michael A Pickard
Keyword(s):  
Rice Bran ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
Scale Up ◽  
Growth Conditions ◽  
White Rot ◽  
Stirred Tank ◽  
Bjerkandera Adusta ◽  
Stirred Tank Reactors ◽  
Cereal Bran

We were looking for a strain of Bjerkandera adusta that produces high titres of manganese peroxidase under optimal conditions for large-scale enzyme purification. We have chosen two strains from the University of Alberta Microfungus Collection and Herbarium, UAMH 7308 and 8258, and compared the effects of growth conditions and medium composition on enzyme production with the well-characterized strain BOS55 (ATCC 90940). Of four types of cereal bran examined, rice bran at 3% (w/v) in 60 mM phosphate buffer pH 6 supported the highest levels of enzyme production. Using 100 mL medium in 500-mL Erlenmeyer flasks, maximum enzyme levels in the culture supernatant occurred after about 10 days of growth; 5.5 U·mL–1 for UAMH 7308, 4.4 U·mL–1 for UAMH 8258, and 1.7 U·mL–1 for BOS55, where units are expressed as micromoles of Mn-malonate formed per minute. Growth as submerged cultures in 10-L stirred tank reactors produced 3.5 U·mL–1 of manganese peroxidase (MnP) by UAMH 8258 and 2.5 U·mL–1 of MnP by 7308, while enzyme production by BOS55 was not successful in stirred tank reactors but could be scaled up in 2-L shake flasks containing 400 mL rice bran or glucose – malt – yeast extract (GMY) – Mn-glycolate medium to produce MnP levels of 1.7 U·mL–1. These results show that the two strains of B. adusta, UAMH 7308 and 8258, can produce between two and three times the manganese peroxidase level of B. adusta BOS55, that they are good candidates for scale up of enzyme production, and that the rice bran medium supports higher levels of enzyme production than most previously described media.Key words: growth conditions, cereal bran, manganese peroxidase, Bjerkandera adusta, white rot fungi.

Production of laccase and manganese peroxidase by white-rot fungi from sugarcane bagasse in solid bed: Use for dyes decolourisation

Sugar Tech ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 260-264 ◽  
Author(s):  
Gilda Guerra ◽  
Osmel Domínguez ◽  
Miguel Ramos-Leal ◽  
Ana M. Manzano ◽  
María I. Sánchez ◽  
...  
Keyword(s):  
Sugarcane Bagasse ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
White Rot
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