Efficacy of acidic and basic electrolyzed water in eradicating Staphylococcus aureus biofilm

2012 ◽  
Vol 58 (4) ◽  
pp. 448-454 ◽  
Author(s):  
Ji-Lu Sun ◽  
Shao-Kang Zhang ◽  
Jing-Yu Chen ◽  
Bei-Zhong Han
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Medical Devices ◽  
Electrolyzed Water ◽  
Viability Assay ◽  
Diphenyltetrazolium Bromide ◽  
Biofilm Architecture ◽  
Novel Method ◽  
Scanning Electron

Staphylococcus aureus is a major pathogen. It can form biofilm on the surfaces of medical devices and food equipment, which makes it more difficult to eradicate. To develop a novel method to eradicate S. aureus biofilm, the effects of electrolyzed water on removing and killing S. aureus biofilm were investigated in this study. By using a biofilm biomass assay with safranin staining and visualization of biofilm architecture with scanning electron microscopy, it was shown that basic electrolyzed water (BEW) could effectively remove established biofilm. The pH of electrolyzed water affected removal efficacy. Using a biofilm viability assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide staining, acidic electrolyzed water (AEW) efficiently killed biofilm-imbedded S. aureus. The available chlorine in AEW may be a main contributing factor for bactericidal activity. Additionally, BEW had a removal efficacy for S. aureus biofilm equivalent to 2% NaOH, and AEW had a bactericidal capability for S. aureus in biofilm equivalent to 2% HCl. These data suggested that AEW and BEW could be applied as a bactericide and removing agent for S. aureus in biofilm, respectively.

Biological Evaluation of Selected 1,2,3-triazole Derivatives as Antibacterial and Antibiofilm Agents

2020 ◽  
Vol 20 (24) ◽  
pp. 2186-2191
Author(s):  
Lialyz Soares Pereira André ◽  
Renata Freire Alves Pereira ◽  
Felipe Ramos Pinheiro ◽  
Aislan Cristina Rheder Fagundes Pascoal ◽  
Vitor Francisco Ferreira ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Antimicrobial Agents ◽  
Public Health Problem ◽  
Biological Evaluation ◽  
Major Public Health Problem ◽  
Community Environments ◽  
Scanning Electron

Background: Resistance to antimicrobial agents is a major public health problem, being Staphylococcus aureus prevalent in infections in hospital and community environments and, admittedly, related to biofilm formation in biotic and abiotic surfaces. Biofilms form a complex and structured community of microorganisms surrounded by an extracellular matrix adhering to each other and to a surface that gives them even more protection from and resistance against the action of antimicrobial agents, as well as against host defenses. Methods: Aiming to control and solve these problems, our study sought to evaluate the action of 1,2,3- triazoles against a Staphylococcus aureus isolate in planktonic and in the biofilm form, evaluating the activity of this triazole through Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests. We have also performed cytotoxic evaluation and Scanning Electron Microscopy (SEM) of the biofilms under the treatment of the compound. The 1,2,3-triazole DAN 49 showed bacteriostatic and bactericidal activity (MIC and MBC 128 μg/mL). In addition, its presence interfered with the biofilm formation stage (1/2 MIC, p <0.000001) and demonstrated an effect on young preformed biofilm (2 MICs, p <0.05). Results: Scanning Electron Microscopy images showed a reduction in the cell population and the appearance of deformations on the surface of some bacteria in the biofilm under treatment with the compound. Conclusion: Therefore, it was possible to conclude the promising anti-biofilm potential of 1,2,3-triazole, demonstrating the importance of the synthesis of new compounds with biological activity.

scholarly journals SINTESIS NANOKOMPOSIT Nata de coco/TiO2/Ag DAN EFEKTIVITASNYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Heriyanto Tinentang ◽  
Henry F Aritonang ◽  
Harry S. J. Koleangan
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Chemical Reduction ◽  
Bacterial Activity ◽  
Energy Dispersive ◽  
X Ray ◽  
Escherichia Coli Bacteria ◽  
Scanning Electron

Telah dilakukan penelitian tentang kemampuan aktivitas anti bakteri untuk bakteri Staphylococcus aureus (gram positif) dan Escherichia coli (gram negatif) dengan menggunakan nanokomposit nata de coco/TiO2, nata de coco/Ag, dan nata de coco/TiO2/Ag dengan variasi konsentrasi Ag 0,5 M; 0,6 M; 0,7 M; 0,8 M dan 0,9 M  menggunakan metode reduksi kimia. Nanopartikel tersebut dikarakterisasi menggunakan X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy  (SEM-EDS) dan anti bakteri. Untuk uji aktivitas antibakteri menggunakan metode kertas cakram dan dilakukan sebanyak dua kali ulangan untuk tiap-tiap sampel dan bakteri yang diuji. Hasil penelitian menunjukan, aktivitas anti bakteri nanokomposit yang paling baik dalam menghambat pertumbuhan bakteri adalah nanokomposit Nata de coco/TiO2/Ag mampu menghambat pertumbuhan bakteri Escherichiacoli dan Staphylococcusaureus, namun nanokomposit tersebut lebih efektif menghambat pertumbuhan bakteri Escherichiacoli.ABSRACT Research on the ability of anti-bacterial activity for Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) bacteria using nata de coco / nanocomposites TiO2, nata de coco / Ag, and nata de coco / TiO2 / Ag with variations of Ag 0,5 M; 0.6 M; 0.7 M; 0.8 M and 0.9 M using the chemical reduction method. Nanoparticles were characterized using X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spctroscopy  (SEM-EDS) and anti-bacterial actvity. Test the antibacterial activity using the paper disc method and repeated two times for each sample and bacteria tested. The results showed that the good anti-bacterial activity of nanocomposites in inhibiting bacterial growth was nanocomposite nata de coco /TiO2/Ag  able to inhibit the growth of Escherichia coli and S. aureus, but the nanocomposite is more effective in inhibiting the growth of Escherichia  coli bacteria.

The Characterization of Bovine Bone-Derived Hydroxyapatite Isolated Using Novel Non-Hazardous Method

2020 ◽  
Vol 45 ◽  
pp. 49-56
Author(s):  
Aniek Setiya Budiatin ◽  
Samirah ◽  
Maria Apriliani Gani ◽  
Wenny Putri Nilamsari ◽  
Chrismawan Ardianto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Extraction Method ◽  
Bovine Bone ◽  
X Ray Diffraction ◽  
X Ray ◽  
Ft Ir ◽  
Novel Method ◽  
Scanning Electron

Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800°C, 900°C and 1,000°C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 ± 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.

A novel method for assessing the role of iron and its functional chelation in fibrin fibril formation: the use of scanning electron microscopy

2013 ◽  
Vol 23 (5) ◽  
pp. 352-359 ◽  
Author(s):  
Etheresia Pretorius ◽  
Natasha Vermeulen ◽  
Janette Bester ◽  
Boguslaw Lipinski ◽  
Douglas B. Kell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fibril Formation ◽  
Novel Method ◽  
Scanning Electron

Influence of Titanium Alloying Element Substrata on Bacterial Adhesion

2012 ◽  
Vol 535-537 ◽  
pp. 992-995
Author(s):  
Kun Mediaswanti ◽  
Vi Khanh Truong ◽  
Jafar Hasan ◽  
Elena P. Ivanova ◽  
Francois Malherbe ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Bacterial Adhesion ◽  
Infection Risk ◽  
Bacterial Attachment ◽  
Bacterial Strains ◽  
Alloying Element ◽  
Bacteria Adhesion ◽  
Scanning Electron

Titanium and titanium alloys have been widely employed in many load-bearing orthopaedic applications due to their excellent strength and corrosion resistance. However, postimplantation infections might occur even though considerable studies have been made. Choosing a bio-friendly alloying element is one way to reduce infection risk. The aim of this study is to evaluate the extent of bacterial attachment on titanium, tantalum, niobium and tin surfaces. Two pathogenic bacterial strains, namely Staphylococcus aureus CIP 65.8T and Pseudomonas aeruginosa ATCC 9027, were used in this study. Quantification of bacterial attachment was performed using scanning electron microscopy. Results indicated that the surface chemistry and topography of the investigated materials significantly influence the degree of P. aeruginosa and S. aureus adhesion; however, surface wettability did not show a significant impact upon bacterial retention. In this study, tin was shown to be the most attractive material for bacteria adhesion but tantalum limits the bacterial adhesion. Therefore, it is suggested to limit the amount of tin as an titanium alloying element due to its nature to attract P. aeruginosa and S. aureus adhesion.

scholarly journals Ultrastructural Study of Elaeis guineensis (Oil Palm) Leaf and Antimicrobial Activity of its Methanol Extract against Staphylococcus Aureus

2016 ◽  
Vol 12 (3) ◽  
pp. 419-423 ◽  
Author(s):  
Amala Rajoo ◽  
Sreenivasan Sasidharan ◽  
Subramanion L Jothy ◽  
Surash Ramanathan ◽  
Sharif M Mansor
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Transmission Electron Microscopy ◽  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Elaeis Guineensis ◽  
Transmission Electron ◽  
Inhibition Concentration ◽  
Scanning Electron

Purpose: To evaluate the antimicrobial activity of the methanol extract of Elaeis guineensis leaf against Staphylococcus aureus and to determine the effect of extract treatment on the microstructure of the microbeMethods: The antimicrobial activity of the methanol leaf extract of the plant against S. aureus was examined using disc diffusion and broth dilution methods. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were carried out to determine the major alterations in the microstructure of S. aureus after treatment with the extract.Results: The extract showed a good antimicrobial activity against S. aureus with a minimum inhibition concentration (MIC) of 6.25 mg/mL and for Chloramphenicol was 30.00 ìg/mL. The main changes observed under SEM and TEM were structural disorganization of the cell membrane which occurred after 12 h and total collapse of the cell 36 h after exposure to the extract.Conclusion: We concluded that the methanolic extract of E. guineensis leaf exhibited good antimicrobial activity against S. aureus and this is supported by SEM and TEM.Keywords: Antimicrobial activity, Elaeis guineensis, Staphylococcus aureus, Scanning electron microscopy, Transmission electron microscopy

Preparation of Polyaniline @ Polypyrrole Conductive Composite via In Situ Polymerization

2013 ◽  
Vol 562-565 ◽  
pp. 1137-1142
Author(s):  
Hui Xia Feng ◽  
Bing Wang ◽  
Lin Tan ◽  
Na Li Chen
Keyword(s):  
Electron Microscopy ◽  
Electrical Conductivity ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
In Situ Polymerization ◽  
Conductive Composite ◽  
The Core ◽  
Novel Method ◽  
Scanning Electron

We prepared the polyaniline@polypyrrole (PAn@PPy) conductive composite by a novel method. The struction like Pre-prepared PAn as the core and PPy as the shell for the composite has been prepared by in-situ polymerization. The PAn@PPy conductive composite presents an electrical conductivity of 12.5 S/cm, which is much higher than pure PAn. The synthesized polymer composites are characterized by Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Thermogravimetric analysis (TG). The results indicated that PPy successfully grafted on PAn and the heat resistance of nanocomposite is remarkably increased.

scholarly journals Study of Staphylococcus aureus Biofilm Ability to Destroy Bone Matrix in Absences of Host Immunity

2020 ◽  
Author(s):  
ahmed Al Ghaithi ◽  
Sultan Al Mastari ◽  
John Husband ◽  
Mohammed al kindi ◽  
Atika Al Bimani
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Raman Spectroscopy ◽  
Immune System ◽  
Bone Resorption ◽  
Bone Quality ◽  
Host Cells ◽  
Immune System Activation ◽  
Scanning Electron

AbstractPurposeOsteomyelitis is an infectious bone process leading to bone necrosis and destruction. Published reports on pathogen biofilm thus far have focused on indirect bone resorption mediated by host cells and factors secondary to immune system activation. However, direct bone resorption due to biofilm pathogen has not been adequately studied yet. This study aims to investigate the effect of biofilm pathogen in ex-vivo human bones in the absence of the host immune response using Raman spectroscopy and Scanning electron microscopy.MethodsBone samples collected from patients who underwent knee replacement surgeries were inoculated with Staphylococcus aureus bacteria. Bacterial direct effects on the bone quality were then examined, at various time intervals, using Raman spectroscopy and scanning electron microscopy.ResultsRaman spectroscopy and scanning electron demonstrated the destruction of bone structure and drop in bone quality.ConclusionThis experiment shows the direct effect of bacteria on bone during osteomyelitis in addition to the recognised destruction caused by the host immune system.

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