Proteomic insight into phenolic adaptation of a moderately halophilicHalomonassp. strain AAD12

2011 ◽  
Vol 57 (4) ◽  
pp. 295-302 ◽  
Author(s):  
Selim Ceylan ◽  
Berna Sarıyar Akbulut ◽  
Aziz Akın Denizci ◽  
Dilek Kazan
Keyword(s):  
Protein Biosynthesis ◽  
Halophilic Bacteria ◽  
General Mechanism ◽  
Lag Phase ◽  
Spectrometric Analysis ◽  
Rrna Gene ◽  
Gram Negative ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Moderately Halophilic Bacteria

A gram-negative, moderately halophilic bacterium was isolated from Çamaltı Saltern area, located in the Aegean Region of Turkey. Analysis of its 16S rRNA gene sequence and physiological characteristics showed that this strain belonged to the genus Halomonas ; hence, it was designated as Halomonas sp. strain AAD12. The isolate tolerated up to 800 mg⋅L–1phenol; however, at elevated concentrations, phenol severely retarded cell growth. The increase in lag phase with increasing phenol concentrations indicated that the microorganism was undergoing serious adaptative changes. To understand the physiological responses of Halomonas sp. strain AAD12 to phenol, a 2-dimensional electrophoresis approach combined with mass spectrometric analysis was used. This approach showed that the expression of 14 protein spots were altered as phenol concentration increased from 200 to 800 mg⋅L–1. Among the identified proteins were those involved in protein biosynthesis, energy, transport, and stress metabolism. So far, this is the first study on phenolic adaptation of a gram-negative, moderately halophilic bacteria using proteomic tools. The results provided new insights for understanding the general mechanism used by moderately halophilic bacteria to tolerate phenol and suggested the potential for using these microorganisms in bioremediation.

scholarly journals Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium

2005 ◽  
Vol 55 (5) ◽  
pp. 1789-1795 ◽  
Author(s):  
María Teresa García ◽  
Virginia Gallego ◽  
Antonio Ventosa ◽  
Encarnación Mellado
Keyword(s):  
Enrichment Culture ◽  
Phylogenetic Analyses ◽  
Halophilic Bacteria ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Gram Positive ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
Wide Range

A novel moderately halophilic bacterium, strain G-19.1T, has been isolated from a phenol enrichment of samples collected in hypersaline habitats of southern Spain. This enrichment culture was a part of a screening programme to isolate halophilic bacteria able to degrade various aromatic compounds. Strain G-19.1T has been characterized as a potential phenol-degrader over a wide range of saline conditions. Strain G-19.1T was found to be an aerobic, Gram-positive, endospore-forming, non-pigmented, moderately halophilic rod that grew optimally in media containing 7·5–10 % NaCl at pH 7·0. The DNA G+C content was 42·4 mol%. Phylogenetic analysis based on comparison of 16S rRNA gene sequences indicated that the closest relatives were Halobacillus species (96·2–97·0 %), although this novel isolate constitutes a separate line of descent within the radiation of Gram-positive rods. The cell-wall peptidoglycan contained meso-diaminopimelic acid, indicating that this strain does not share the main characteristic that differentiates members of the genus Halobacillus (which contain Orn–d-Asp) from other related genera. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. On the basis of phenotypic, genotypic and phylogenetic analyses, this isolate should be classified in a novel genus and species, for which the name Thalassobacillus devorans gen. nov., sp. nov. is proposed. The type strain is strain G-19.1T (=DSM 16966T=CECT 7046T=CCM 7282T).

scholarly journals Megaplasmids in Gram-negative, moderately halophilic bacteria

2003 ◽  
Vol 227 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Montserrat Argandoña ◽  
Fernando Martı́nez-Checa ◽  
Inmaculada Llamas ◽  
Emilia Quesada ◽  
Ana Moral
Keyword(s):  
Halophilic Bacteria ◽  
Gram Negative ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacteria

scholarly journals From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov.

2014 ◽  
Vol 80 (13) ◽  
pp. 3850-3857 ◽  
Author(s):  
María José León ◽  
Ana B. Fernández ◽  
Rohit Ghai ◽  
Cristina Sánchez-Porro ◽  
Francisco Rodriguez-Valera ◽  
...  
Keyword(s):  
Pure Culture ◽  
Culture Media ◽  
Halophilic Bacteria ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Moderately Halophilic ◽  
Isolation And Characterization ◽  
Moderately Halophilic Bacterium

ABSTRACTRecent metagenomic studies on saltern ponds with intermediate salinities have determined that their microbial communities are dominated by bothEuryarchaeotaand halophilic bacteria, with a gammaproteobacterium closely related to the generaAlkalilimnicolaandArhodomonasbeing one of the most predominant microorganisms, making up to 15% of the total prokaryotic population. Here we used several strategies and culture media in order to isolate this organism in pure culture. We report the isolation and taxonomic characterization of this new, never before cultured microorganism, designated M19-40T, isolated from a saltern located in Isla Cristina, Spain, using a medium with a mixture of 15% salts, yeast extract, and pyruvic acid as the carbon source. Morphologically small curved cells (young cultures) with a tendency to form long spiral cells in older cultures were observed in pure cultures. The organism is a Gram-negative, nonmotile bacterium that is strictly aerobic, non-endospore forming, heterotrophic, and moderately halophilic, and it is able to grow at 10 to 25% (wt/vol) NaCl, with optimal growth occurring at 15% (wt/vol) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that strain M19-40Thas a low similarity with other previously described bacteria and shows the closest phylogenetic similarity with species of the generaAlkalilimnicola(94.9 to 94.5%),Alkalispirillum(94.3%), andArhodomonas(93.9%) within the familyEctothiorhodospiraceae. The phenotypic, genotypic, and chemotaxonomic features of this new bacterium showed that it constitutes a new genus and species, for which the nameSpiribacter salinusgen. nov., sp. nov., is proposed, with strain M19-40T(= CECT 8282T= IBRC-M 10768T= LMG 27464T) being the type strain.

Study on biocharacteristics and puruvate production of moderately halophilic bacteria isolated from dunalliella tertiolecta’s culture medium

2018 ◽  
Vol 40 (4) ◽  
Author(s):  
Hoang Thi Lan Anh ◽  
Luu Thi Tam ◽  
Dang Diem Hong
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Culture Medium ◽  
Sequence Similarity ◽  
Halophilic Bacteria ◽  
Rrna Gene ◽  
Wild Type ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacteria

A moderately halophilic bacteria designed strain D34 was isolated from the culture medium of green microalga Dunaliella tertiolecta. The isolate was Gram-negative, aerobic, rod-shaped, approximately 0.45–0.60 mm wide and 1.25–5.10 mm long, occuring singly, non-motile, and flagellum- less. Colonies on solid media are cream, circular, and smooth. This strain was able to produce exopolysaccharide, poly hydroxybutyrate, oxidase and catalase positive. Growth occurred in a temparature range of 20–40°C, a salts concentration of 0.1–25% (w/v), and pH range 6–12. The major fatty acids were C16:0 (35.59%), C16:1w-7 (20.54%), C18: 1w-7 (30.14%), and C12:0 (10.03% of total fatty acids). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain D34 belonged to the genus Halomonas. The highest levels of 16S rRNA gene sequence similarity were found between the strain D34 and H. aquamarina (sequence similarity 98.6 %).Pyruvate, a central intermediate in metabolism processes in all organisms, is widely used for the synthesis of various chemicals and polymers as well as ingredient or additive in food, cosmetics, and pharmaceuticals. In this study, pyruvate production by strain D34 following changes in culture medium, glucose and nitrate concentrations and culture temperature were also studied. In 84 hours of batch- cultivation, pyruvate production by wild-type Halomonas sp. D34 reached 37.24 g/L at 37°C with 20% glucose and 30 g/L sodium nitrate adding to SOT medium. These data provided evidences for pyruvate production using novel wild-type strains. 

scholarly journals Chromohalobacter nigrandesensis sp. nov., a moderately halophilic, Gram-negative bacterium isolated from Lake Tebenquiche on the Atacama Saltern, Chile

2006 ◽  
Vol 56 (3) ◽  
pp. 647-651 ◽  
Author(s):  
Bernardo Prado ◽  
Catherine Lizama ◽  
Margarita Aguilera ◽  
Alberto Ramos-Cormenzana ◽  
Susana Fuentes ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Halophilic Bacteria ◽  
Rrna Gene ◽  
Gram Negative ◽  
Moderately Halophilic ◽  
Nutritional Characteristics ◽  
Ph Range ◽  
Black Pigmentation

A total of 52 strains of moderately halophilic bacteria isolated from hypersaline sediment of Lake Tebenquiche on the Atacama Saltern, Chile, were subjected to a taxonomic study. The morphological, physiological, biochemical and nutritional characteristics of the strains matched those described for the genus Chromohalobacter. Cells were Gram-negative, non-spore-forming, rod-shaped and motile. A black pigmentation was produced. One strain, designated LTS-4NT, grew optimally at 32 °C. Growth occurred in media containing 0·5–25 % (w/v) total salts; the optimum was 7·5 % (w/v) total salts. The pH range for growth was 5–10. The G+C content of the DNA of strain LTS-4NT was 59·8 mol%. Analysis of 16S rRNA gene sequence similarity revealed that strain LTS-4NT was closely related to Chromohalobacter species; however, DNA–DNA hybridization of representative strain LTS-4NT failed to associate the strain with any species of the genus Chromohalobacter with validly published names. Therefore, the name Chromohalobacter nigrandesensis sp. nov. is proposed. The type strain is LTS-4NT (=CECT 5315T=DSM 14323T).

scholarly journals Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China

2005 ◽  
Vol 55 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Pei-Gen Ren ◽  
Pei-Jin Zhou
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Salt Lake ◽  
Sequence Similarity ◽  
Halophilic Bacteria ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Neutral Salt ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

Two Gram-positive, rod-shaped, spore-forming and moderately halophilic bacteria (strains 28-1T, 28-4), isolated from a soil sample from a neutral salt lake in Xin-Jiang, China, were characterized polyphasically. On the basis of fasta (ungapped) analyses of 16S rRNA gene sequences, strains 28-1T and 28-4 were shown to belong to the Bacillaceae and to be closely related to Filobacillus milensis DSM 13259T (97·0 %) and Bacillus haloalkaliphilus DSM 5271T (95·7 %). 16S rRNA gene sequence similarity with other recognized species was not more than 94·1 %. Phylogenetic, chemotaxonomic, physiological and biochemical data supported the differentiation of these novel strains from F. milensis and B. haloalkaliphilus. Therefore these two previously unidentified strains are considered to represent a new genus and species, for which the name Tenuibacillus multivorans gen. nov., sp. nov. is proposed. The type strain is 28-1T (=AS 1.3442T=NBRC 100370T).

scholarly journals Halomonas urmiana sp. nov., a moderately halophilic bacterium isolated from Urmia Lake in Iran

2020 ◽  
Vol 70 (4) ◽  
pp. 2254-2260 ◽  
Author(s):  
Shehzad Abid Khan ◽  
Sepideh Zununi Vahed ◽  
Haleh Forouhandeh ◽  
Vahideh Tarhriz ◽  
Nader Chaparzadeh ◽  
...  
Keyword(s):  
Type Species ◽  
Halophilic Bacteria ◽  
Polar Lipids ◽  
Urmia Lake ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Moderately Halophilic ◽  
Link Type ◽  
Moderately Halophilic Bacterium

In the course of screening halophilic bacteria in Urmia Lake in Iran, which is being threatened by dryness, a novel Gram-negative, moderately halophilic, heterotrophic and short rod-shaped bacteria was isolated and characterized. The bacterium was isolated from a water specimen and designated as TBZ3T. Colonies were found to be creamy yellow, with catalase- and oxidase-positive activities. The growth of strain TBZ3T was observed to be at 10–45 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 0.5–20 % (w/v) NaCl (optimum, 7.5 %). Strain TBZ3T contained C16 : 0, cyclo-C19 : 0  ω8c, summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c) and summed feature 8 (comprising C18 : 1  ω7c and/or C18 : 1  ω6c) as major fatty acids and ubiquinone-9 as the only respiratory isoprenoid quinone. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, glycolipid, unidentified phospholipid and unidentified polar lipids were detected as the major polar lipids. Strain TBZ3T was found to be most closely related to Halomonas saccharevitans AJ275T , Halomonas denitrificans M29T and Halomonas sediminicola CPS11T with the 16S rRNA gene sequence similarities of 98.93, 98.15 and 97.60 % respectively and in phylogenetic analysis strain TBZ3T grouped with Halomonas saccharevitans AJ275T contained within a large cluster within the genus Halomonas . Based on phenotypic, chemotaxonomic and molecular properties, strain TBZ3T represents a novel species of the Halomonas genus, for which the name Halomonas urmiana sp. nov. is proposed. The type strain is TBZ3T (=DSM 22871T=LMG 25416T).

scholarly journals Streptohalobacillus salinus gen. nov., sp. nov., a moderately halophilic, Gram-positive, facultative anaerobe isolated from subsurface saline soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1127-1132 ◽  
Author(s):  
Xiaowei Wang ◽  
Yanfen Xue ◽  
Yanhe Ma
Keyword(s):  
Saline Soil ◽  
Qaidam Basin ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
The Family ◽  
Diamino Acid

A Gram-stain-positive, rod-shaped, non-sporulating, motile and moderately halophilic bacterium, designated strain H96B60T, was isolated from a saline soil sample of the Qaidam basin, China. The strain was facultatively anaerobic. Major end products formed from glucose fermentation were acetate, ethanol and lactic acid. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone component was menaquinone-6 (MK-6). The predominant cellular fatty acids were C16 : 0, anteiso-C13 : 0 and anteiso-C15 : 0. The genomic DNA G+C content of strain H96B60T was 36.2 mol%. Phylogenetic analysis based on comparative 16S rRNA gene sequences indicated that strain H96B60T represented a novel phyletic lineage within the family Bacillaceae and was related most closely to Halolactibacillus species (96.1–96.4 % similarity). Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain H96B60T is considered to represent a novel species of a new genus, for which the name Streptohalobacillus salinus gen. nov., sp. nov. is proposed. The type strain of Streptohalobacillus salinus is H96B60T ( = DSM 22440T  = CGMCC 1.7733T).

scholarly journals Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep

2007 ◽  
Vol 57 (6) ◽  
pp. 1243-1249 ◽  
Author(s):  
Ngoc-Phuc Hua ◽  
Atsuko Kanekiyo ◽  
Katsunori Fujikura ◽  
Hisato Yasuda ◽  
Takeshi Naganuma
Keyword(s):  
Deep Sea ◽  
Sequence Similarity ◽  
Halophilic Bacteria ◽  
Cold Seep ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
High Sequence Similarity ◽  
Moderately Halophilic ◽  
Type Strains ◽  
Moderately Halophilic Bacteria

Two Gram-positive, rod-shaped, moderately halophilic bacteria were isolated from a deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll, Japan. These bacteria, strains IS-Hb4T and IS-Hb7T, were spore-forming and non-motile. They were able to grow at temperatures as low as 9 °C and hydrostatic pressures up to 30 MPa. Based on high sequence similarity of their 16S rRNA genes to those of type strains of the genus Halobacillus, from 96.4 % (strain IS-Hb7T to Halobacillus halophilus NCIMB 9251T) to 99.4 % (strain IS-Hb4T to Halobacillus dabanensis D-8T), the strains were shown to belong to this genus. DNA–DNA relatedness values of 49.5 % and 1.0–33.0 %, respectively, were determined between strains IS-Hb4T and IS-Hb7T and between these strains and other Halobacillus type strains. Both strains showed the major menaquinone MK7 and l-orn–d-Asp cell-wall peptidoglycan type. Straight-chain C16 : 0, unsaturated C16 : 1 ω7c alcohol and C18 : 1 ω7c and cyclopropane C19 : 0 cyc fatty acids were predominant in both strains. The DNA G+C contents of IS-Hb4T and IS-Hb7T were respectively 43.3 and 42.1 mol%. Physiological and biochemical analyses combined with DNA–DNA hybridization results allowed us to place strains IS-Hb4T (=JCM 14154T=DSM 18394T) and IS-Hb7T (=JCM 14155T=DSM 18393T) in the genus Halobacillus as the respective type strains of the novel species Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov.

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