Differentially expressed genes after hyper- and hypo-salt stress in the halophilic archaeonMethanohalophilus portucalensis

2010 ◽  
Vol 56 (4) ◽  
pp. 295-307 ◽  
Author(s):  
Chao-Jen Shih ◽  
Mei-Chin Lai
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Differentially Expressed Genes ◽  
Transmembrane Protein ◽  
Stress Protein ◽  
Differentially Expressed ◽  
Transcriptional Analysis ◽  
Salt Stress Response ◽  
Salt Shock

Methanohalophilus portucalensis FDF1 can grow over a range of external NaCl concentrations, from 1.2 to 2.9 mol/L. Differential gene expression in response to long-term hyper-salt stress (3.1 mol/L of NaCl) and hypo-salt stress (0.9 mol/L of NaCl) were compared by differential display RT-PCR. Fourteen differentially expressed genes responding to long-term hyper- or hypo-salt stress were detected, cloned, and sequenced. Several of the differentially expressed genes were related to the unique energy-acquiring methanogenesis pathway in this organism, including the transmembrane protein MttP, cobalamin biosynthesis protein, methenyl-H4MPT cyclohydrolase and monomethylamine methyltransferase. One signal transduction histidine kinase was identified from the hyper-salt stress cultures. Moreover, 3 known stress-response gene homologues — the DNA mismatch repair protein, MutS, the universal stress protein, UspA, and a member of the protein-disaggregating multichaperone system, ClpB — were also detected. The transcriptional analysis of these long-term salt stress response and adaptation-related genes for cells immediately after salt stress indicated that the expression of the energy metabolism genes was arrested during hyper-salt shock, while the chaperone clpB gene was stimulated by both hypo- and hyper-salt shock.

scholarly journals Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12492
Author(s):  
Xun Liu ◽  
Xinxia Yang ◽  
Bin Zhang
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Tolerance ◽  
Transcriptome Analysis ◽  
Molecular Mechanisms ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Functional Identification ◽  
Salt Stress Response ◽  
Soybean Seedlings

Salinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcriptome analysis revealed 1,235 differentially expressed genes (DEGs) under salt stress. Several important pathways and key candidate genes were identified by KEGG enrichment. A total of 116 differentially expressed transcription factors (TFs) were identified, and 17 TFs were found to belong to MYB families. Phylogenetic analysis revealed that these TFs may be involved in salt stress adaptation. Further analysis revealed that GmMYB46 was up-regulated by salt and mannitol and was localized in the nucleus. The salt tolerance of transgenic Arabidopsis overexpressing GmMYB46 was significantly enhanced compared to wild-type (WT). GmMYB46 activates the expression of salt stress response genes (P5CS1, SOD, POD, NCED3) in Arabidopsis under salt stress, indicating that the GmMYB46 protein mediates the salt stress response through complex regulatory mechanisms. This study provides information with which to better understand the molecular mechanism of salt tolerance in soybeans and to genetically improve the crop.

The role of antioxidative metabolism of tomato leaves in long-term salt-stress response

2017 ◽  
Vol 180 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Ivana Koleška ◽  
Dino Hasanagić ◽  
Ivana Maksimović ◽  
Borut Bosančić ◽  
Biljana Kukavica
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response ◽  
Antioxidative Metabolism

scholarly journals Short-Term Temporal Metabolic Behavior in Halophilic Cyanobacterium Synechococcus sp. Strain PCC 7002 after Salt Shock

Metabolites ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 297 ◽  
Author(s):  
Shimpei Aikawa ◽  
Atsumi Nishida ◽  
Tomohisa Hasunuma ◽  
Jo-Shu Chang ◽  
Akihiko Kondo
Keyword(s):  
Gene Expression ◽  
Mass Spectrometry ◽  
Salt Stress ◽  
Stress Response ◽  
Tricarboxylic Acid Cycle ◽  
Compatible Solutes ◽  
Uptake System ◽  
Salt Stress Response ◽  
Salt Shock ◽  
Synechococcus Sp

In response to salt stress, cyanobacteria increases the gene expression of Na+/H+ antiporter and K+ uptake system proteins and subsequently accumulate compatible solutes. However, alterations in the concentrations of metabolic intermediates functionally related to the early stage of the salt stress response have not been investigated. The halophilic cyanobacterium Synechococcus sp. PCC 7002 was subjected to salt shock with 0.5 and 1 M NaCl, then we performed metabolomics analysis by capillary electrophoresis/mass spectrometry (CE/MS) and gas chromatography/mass spectrometry (GC/MS) after cultivation for 1, 3, 10, and 24 h. Gene expression profiling using a microarray after 1 h of salt shock was also conducted. We observed suppression of the Calvin cycle and activation of glycolysis at both NaCl concentrations. However, there were several differences in the metabolic changes after salt shock following exposure to 0.5 M and 1 M NaCl: (i): the main compatible solute, glucosylglycerol, accumulated quickly at 0.5 M NaCl after 1 h but increased gradually for 10 h at 1 M NaCl; (ii) the oxidative pentose phosphate pathway and the tricarboxylic acid cycle were activated at 0.5 M NaCl; and (iii) the multi-functional compound spermidine greatly accumulated at 1 M NaCl. Our results show that Synechococcus sp. PCC 7002 acclimated to different levels of salt through a salt stress response involving the activation of different metabolic pathways.

scholarly journals Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress

2021 ◽  
Vol 22 (11) ◽  
pp. 5957
Author(s):  
Hyun Jin Chun ◽  
Dongwon Baek ◽  
Byung Jun Jin ◽  
Hyun Min Cho ◽  
Mi Suk Park ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Salt Stress ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Related Gene ◽  
Plant Adaptation ◽  
Salt Stress Response ◽  
Network Analyses ◽  
Cell Wall Biogenesis

Although recent studies suggest that the plant cytoskeleton is associated with plant stress responses, such as salt, cold, and drought, the molecular mechanism underlying microtubule function in plant salt stress response remains unclear. We performed a comparative proteomic analysis between control suspension-cultured cells (A0) and salt-adapted cells (A120) established from Arabidopsis root callus to investigate plant adaptation mechanisms to long-term salt stress. We identified 50 differentially expressed proteins (45 up- and 5 down-regulated proteins) in A120 cells compared with A0 cells. Gene ontology enrichment and protein network analyses indicated that differentially expressed proteins in A120 cells were strongly associated with cell structure-associated clusters, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed that expressions of cytoskeleton-related genes, such as FBA8, TUB3, TUB4, TUB7, TUB9, and ACT7, and a cell wall biogenesis-related gene, CCoAOMT1, were induced in salt-adapted A120 cells. Moreover, the loss-of-function mutant of Arabidopsis TUB9 gene, tub9, showed a hypersensitive phenotype to salt stress. Consistent overexpression of Arabidopsis TUB9 gene in rice transgenic plants enhanced tolerance to salt stress. Our results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress. The modulation of microtubule-related gene expression can be an effective strategy for developing salt-tolerant crops.

Progress in understanding salt stress response in plants using biotechnological tools

2021 ◽  
Vol 329 ◽  
pp. 180-191
Author(s):  
Ulkar İbrahimova ◽  
Pragati Kumari ◽  
Saurabh Yadav ◽  
Anshu Rastogi ◽  
Michal Antala ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Salt Stress Response

scholarly journals Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii

BMC Genomics ◽  
2012 ◽  
Vol 13 (1) ◽  
pp. 215 ◽  
Author(s):  
Guido Mastrobuoni ◽  
Susann Irgang ◽  
Matthias Pietzke ◽  
Heike E Aßmus ◽  
Markus Wenzel ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Chlamydomonas Reinhardtii ◽  
Salt Stress Response ◽  
Photosynthetic Organism
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