Evaluation of nitrogenous media components by Plackett–Burman statistical design for β-d-fructofuranosidase production by Saccharomyces sp. strain GVT263

2009 ◽  
Vol 55 (4) ◽  
pp. 405-409 ◽  
Author(s):  
M. Venkateshwar ◽  
K. Chaitanya ◽  
M. D. Altaf ◽  
B. Hameeda ◽  
M. Ghopal Reddy
Keyword(s):  
Experimental Design ◽  
Yeast Extract ◽  
Ammonium Acetate ◽  
Statistical Design ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Statistical Experimental Design ◽  
Meat Extract ◽  
Medium Components ◽  
Sucrose Fermentation

β-d-Fructofuranosidase (FFase), an important enzyme of the confectionery and fructose syrup industry, is produced by several microorganisms. However, yeasts are the most used source because of their high sucrose fermentation capacity. In this work, production of FFase was carried out in submerged fermentation using a high enzyme-producing yeast strain. Plackett–Burman statistical experimental design was applied to evaluate the fermentation medium components. The effects of 10 nitrogen sources were studied in a 16-run experimental design. Beef extract, yeast extract, N-Z-amine, tryptone, meat extract, and ammonium acetate were found to have significant effects on enzyme production. Among these, yeast extract, N-Z-amine, and ammonium acetate were the most significant. A maximum FFase activity of 299.4 U/mL was obtained after a 24 h fermentation period.

scholarly journals Evaluation of the process conditions for the production of microbial carotenoids by the recently isolated Rhodotorula mucilaginosa URM 7409

2019 ◽  
Vol 22 ◽  
Author(s):  
Whallans Raphael Couto Machado ◽  
Lucas Gomes da Silva ◽  
Ellen Silva Lago Vanzela ◽  
Vanildo Luiz Del Bianchi
Keyword(s):  
Experimental Design ◽  
Yeast Extract ◽  
Culture Medium ◽  
Nitrogen Sources ◽  
Process Conditions ◽  
Malt Extract ◽  
Rhodotorula Mucilaginosa ◽  
Process Characteristics ◽  
Initial Ph ◽  
Microbial Carotenoids

Abstract This study aimed to improve the physical and nutritional process conditions for the production of carotenoids by the newly isolated Rhodotorula mucilaginosa, a red basidiomycete yeast. The carotenoid bioproduction was improved using an experimental design technique, changing the process characteristics of agitation (130 rpm to 230 rpm) and temperature (25 °C to 35 °C) using seven experiments, followed by a 25-1 fractional design to determine the relevant factors that constitute the culture medium (glucose, malt extract, yeast extract, peptone and initial pH). A complete second order experimental design was then carried out to optimize the composition of the culture medium, the variables being yeast extract (0.5 to 3.5 g/L), peptone (1 to 5 g/L) and the initial pH (5.5 to 7.5), with 17 experiments. The maximum carotenoid production was 4164.45 μg/L (252.99 μg/g), obtained in 144 h in YM (yeast malt) medium with 30 g/L glucose, 10 g/L malt extract, 2 g/L yeast extract, 3 g/L peptone, an initial pH 6, 130 rpm and 25 °C, demonstrating the potential of this yeast as a source of bio-pigments. In this work, the nitrogen sources were the factors that most influenced the intracellular accumulation of carotenoids. The yeast R. mucilaginosa presented high production at a bench level and may be promising for commercial production.

Optimization of Medium Components for β-Glucosidase Production from Aspergillus Niger by Response Surface Methodology

2013 ◽  
Vol 419 ◽  
pp. 328-333 ◽  
Author(s):  
Chao Zhang ◽  
Rui Huang ◽  
Hui Tian ◽  
Ru Ming Zhao ◽  
Fa Shun Yu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Influence Factors ◽  
Mutual Effect ◽  
Tween 80 ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Malt Extract ◽  
Medium Components

β-Glucosidase is the key enzyme for the utilization of lignocellulose.But the commercial β-glucosidase can’t be produced. This paper focuses on the study of the β-glucosidase fermentation process.The fermentation medium components for β-glucosidase production from Aspergil lusniger was optimized by response surface methodology (RSM). Firstly, the three of the most important influence factors yeast extract, MnSO4•H2O and MgSO4•7H2O was obtained from Plackett-Burman design screening. Then the path of steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration and mutual effect of three factors were predicted by RSM. The results showed that the best medium composition was Malt extract 18g/L, Yeast extract 3.22g/L, KH2PO4 3g/L, MnSO4•H2O 0.58mM, Tween-80 0.5mL/L and MgSO4•7H2O 0.23g/L. Under these fermentation conditions, the activity of β-glucosidase was up to 7.33IU/mL with increasing 23.2% than before.

scholarly journals Optimization of Penicillium Lagena Medium Cultivication on Antifungal Pathogen of Phellinus Lamaoensis Using Surface Methode

Teknik ◽  
2018 ◽  
Vol 38 (2) ◽  
pp. 58
Author(s):  
Siti Nabilah ◽  
Rofiq Sunaryanto ◽  
Khaswar Syamsu
Keyword(s):  
Yeast Extract ◽  
Active Compound ◽  
Statistical Design ◽  
Medium Composition ◽  
Quadratic Model ◽  
Ageratum Conyzoides ◽  
Medium Components ◽  
Mineral Solution ◽  
Solution Verification ◽  
Rot Disease

Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. lagena active compound by quadratic model was predicted to be 69.233%  with medium composition 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution. Verification value in laboratory is 58.365%, lower 15.7% than its prediction. Optimization increase P. lagena active compound 9 fold compared to unoptimize media.

scholarly journals Influence of Nitrogen Sources on D-Lactic Acid Biosynthesis by Sporolactobacillus laevolacticus DSM 442 Strain

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 78
Author(s):  
Alicja Katarzyna Michalczyk ◽  
Sylwia Garbaczewska ◽  
Bolesław Morytz ◽  
Arkadiusz Białek ◽  
Jerzy Zakrzewski
Keyword(s):  
Lactic Acid ◽  
Nitrogen Source ◽  
Yeast Extract ◽  
Optical Purity ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Agricultural Product ◽  
Acid Biosynthesis ◽  
Batch Cultures ◽  
Pea Seed

The purpose of this study was to explore the possibility of replacing an expensive yeast extract contained in the fermentation medium for D-lactic acid (D-LA, R-lactic acid) biosynthesis with an alternative nitrogen source. The screening studies were conducted under stationary conditions and showed that pea seed hydrolysate was the most beneficial substrate in the process of D-LA biosynthesis by the strain Sporolactobacillus laevolacticus DSM 442 among the used inorganic and organic nitrogen sources, waste materials, food and agricultural products. After 96 h, 75.5 g/L D-LA was obtained in batch cultures in a medium containing pea seed hydrolysate, with an average productivity of 0.79 g/L/h, yield of 75.5%, and optical purity of 99.4%. In batch cultures fed once, in a medium with an analogous composition, 122.6 g/L D-LA was obtained after 120 h, and the average yield, productivity and optical purity were 87.6%, 1.021 g/L/h, and 99.6%, respectively. Moreover, the amount of D-LA obtained in the fermentation medium enriched with the above-mentioned cheap agricultural product was similar to the amounts obtained in the medium containing yeast extract in both stationary and bioreactor cultures. Our research shows that hydrolyzed pea seeds, which belong to the legume family, may be a promising nitrogen source for the production of D-LA on an industrial scale.

scholarly journals Statistical experimental design screening strategies for free monomeric isocyanates determination by UPLC in materials used in cork stoppers manufacturing

ACTA IMEKO ◽  
2017 ◽  
Vol 6 (1) ◽  
pp. 50
Author(s):  
Catarina André ◽  
Inês Delgado ◽  
Isabel Castanheira ◽  
João Bordado ◽  
Ana Sofia Matos
Keyword(s):  
Experimental Design ◽  
Ammonium Acetate ◽  
Ultra Performance Liquid Chromatography ◽  
Toluene Diisocyanate ◽  
Array Detector ◽  
Statistical Experimental Design ◽  
Column Temperature ◽  
Figures Of Merit ◽  
Materials Used ◽  
Cork Stoppers

<p>A statistical experimental design was used to screen variables of the analytical procedure to quantify free monomeric isocyanates presented in polyurethane based pre-polymers in trace amounts.</p><p>For this purpose, diphenylmethane-4,4’-diisocyanate (4,4’-MDI), 2,4-toluene diisocyanate (2,4-TDI) and 2,6-toluene diisocyanate (2,6-TDI) were analysed by Ultra Performance Liquid Chromatography with a Photo Diode Array detector (UPLC-PDA). A preliminary study was performed with three derivatization agents, being 1-(2-piridyl) piperazine (1,2-PP) the most suitable one. Column temperature, flow and percentage of ammonium acetate (% NH4Ac.) were the factors studied at two levels each. A sequence of experiments was planned according to a 23 full factorial design with three replicates and two repetitions. Analysis of variance (ANOVA) was applied for the identification of significant factors and interactions.</p><p>Higher responses were achieved when the column temperature was 30 °C, a flow of 0.3 mL/min and a solvent with a percentage of ammonium acetate of 0.1 %. Figures of merit were assessed within-laboratory as a preliminary step for method validation. Similar values were obtained for TDI and MDI. Recoveries are approximately 100 %. In addition, the values of detection limits (LODs) for MDI and TDI were 0.08 and 0.11 microgram/mL, respectively, and quantification limits (LOQs) were 0.25 and 0.33 microgram/mL for MDI and TDI, respectively. The working range was between 0.01 and 10.00 microgram/mL for MDI and 0.01 – 4.95 microgram/mL for TDI. These figures of merit seemed adequate to detect low amounts of free monomeric isocyanates presented in agglomerates and foams for agglomerated cork stoppers production. This data is suitable to address the optimization of an analytical method by a response surface methodology.</p>

scholarly journals Enhanced Interferon-α2b Production in Periplasmic Space of Escherichia coli through Medium Optimization using Response Surface Method

2009 ◽  
Vol 3 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Joo Shun Tan ◽  
Ramakrishnan Nagasundara Ramanan ◽  
Siti Nor Ani Azaman ◽  
Tau Chuan Ling ◽  
Mustapha Shuhaimi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Response Surface ◽  
Yeast Extract ◽  
Nitrogen Sources ◽  
Luria Bertani ◽  
Human Interferon ◽  
Shake Flask Culture ◽  
Periplasmic Space ◽  
Medium Components ◽  
Interferon Α2b

The influence of different carbon and nitrogen sources on growth of recombinant Escherichia coli and human interferon-α2b (IFN-α2b) production in periplasmic space was studied in shake flask culture. A statistical method based on Plackett-Burman design was used to screen the main medium components that greatly influenced the performance of the fermentation process. The optimization of medium was performed using response surface methodology (RSM) where three critical factors (glucose, yeast extract and peptone) were optimized using central composite design. The highest yield of periplasmic recombinant human interferon-α2b (PrIFN-α2b) (335.8 μg/L) was predicted to be obtained in optimized medium containing 5.47 g/L glucose, 55.24 g/L yeast extract and 42.27 g/L peptone.. The production of IFN-α2b in periplasmic space in optimized medium was about 2.5, 11.7 and 124.4 times higher than Terrific broth (TB), Luria-Bertani (LB), and minimal medium (M9), respectively.

scholarly journals Investigations into Enzymatic Bioconversion to Form Rebaudioside A from Stevioside

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Réka Czinkóczky ◽  
Áron Németh
Keyword(s):  
Experimental Design ◽  
Statistical Design ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Statistical Experimental Design ◽  
Rebaudioside A ◽  
Stevia Rebaudiana Bertoni ◽  
Perennial Shrub ◽  
Enzymatic Bioconversion ◽  
Full Factorial

Stevia rebaudiana Bertoni is a perennial shrub from South America that produces steviol glycosides which are 200-300 times sweeter than sugar. Stevioside and rebaudioside A are the main sweetening components of its leaves. Steviol glycosides are diterpenoids whose biosynthetic pathways have four steps in common with gibberellic acid formation. The most important enzyme in the biosynthetic pathway expressed by the gene UGT76G1 is referred to as UDP-glycosyltransferase 76G1. It converts stevioside into rebaudioside A. The former has a bitter aftertaste and is a poorer sweetener but is most abundant. This enzyme can be produced in a next generation recombinant way by Escherichia coli and Saccharomyces cerevisiae. Trichoderma longibrachiatum produces the enzyme β-1,3-glucanase enzyme, which can perform a transglycosylation between stevioside to gain rebaudioside A. In our study, a full-factorial statistical experimental design that applies different glycosyl donors, temperatures, enzyme-to-substrate ratios and pH's as factors in order to achieve higher Reb A ratios in S. rebaudiana extracts after transglycosylation is reported. The presented statistical design was appropriate to indicate relevant and significant factors, providing a good basis for an upcoming experimental design of a real-world optimization.

scholarly journals OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in The Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 248
Author(s):  
Mekala Venkatachalam ◽  
Alain Shum-Chéong-Sing ◽  
Yanis Caro ◽  
Laurent Dufossé ◽  
Mireille Fouillaud
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Maximum Yield ◽  
Nitrogen Sources ◽  
Industrial Applications ◽  
Fungal Species ◽  
Pigment Production ◽  
Interactive Effects ◽  
Medium Components

Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions. The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications. Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays. The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources. Nutrient combinations were screened through the one-variable-at-a-time (OVAT) analysis. Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L). Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production. A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients. The experimental results were well fitted with the chosen statistical model. The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O. Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.

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