Serological profiling with Chlamycheck, a commercial multiplex recombinant antigen Western blot assay of chlamydial infections

2007 ◽  
Vol 53 (12) ◽  
pp. 1360-1368 ◽  
Author(s):  
Fouzia Radouani ◽  
Julia Maile ◽  
Fotini Betsou
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydophila Pneumoniae ◽  
Test System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elementary Body ◽  
Western Blot Assay ◽  
Recombinant Antigens ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Single Antigen

A new chlamydial test system, the Chlamycheck assay, which uses 4 purified recombinant antigens of Chlamydia trachomatis and Chlamydophila pneumoniae and one antigen of Chlamydophila psittaci , has been developed and commercialized. We investigated the reactivities of the recombinant antigens with sera from a group of 30 patients with acute Chlamydia trachomatis infection, 88 patients consulting for sexually transmitted infections, and 46 patients with serological evidence of Chlamydophila pneumoniae infection. The results obtained from human and infected mouse sera suggest that Chlamycheck serology against multiple proteins may provide additional useful information that is not available by conventional whole elementary body microimmunofluorescence or single-antigen enzyme-linked immunosorbent assay serology. Specific serological profiles were associated with acute versus past Chlamydia trachomatis infection or with Chlamydia trachomatis primo-infection versus infection in a Chlamydophila pneumoniae history context.

scholarly journals Prevalence of Sexually Transmitted Diseases and Transmission of HIV in Dhaka, Bangladesh

2009 ◽  
Vol 3 (1) ◽  
pp. 27-33
Author(s):  
Tahmina Shirin ◽  
Saidur Rahman ◽  
Fareha Jesmin Rabbi ◽  
Md Humayun Kabir ◽  
KZ Mamun
Keyword(s):  
Human Immunodeficiency Virus ◽  
Herpes Simplex ◽  
Chlamydia Trachomatis ◽  
Sexually Transmitted Diseases ◽  
Treponema Pallidum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sexually Transmitted ◽  
Immunodeficiency Virus ◽  
Simplex Virus

The prevalence of sexually transmitted diseases (STDs) among patients attending out patients department of Skin and Venereal diseases of Dhaka Medical College Hospital, Dhaka and Shahid Sohrawardy Hospital, Dhaka was studied. A total of 230 patients were enrolled in the study during the period of July, 2006 to May, 2007. Urethral and endocervical swabs were collected from the participants for detection of Neisseria gonorrheae (by culture), Chlamydia trachomatis (by immunochromatoghraphy) and blood samples for the detection of Treponema pallidum antibody (by rapid plasma regain and Treponema pallidum haemagglutination assay), Herpes simplex virus type 2 antibody (both IgM and IgG by enzyme linked immunosorbent assay) and Human Immunodeficiency virus antibody (by enzyme linked immunosorbent assay). Socio-demographic data and data regarding high-risk sexual behavior were also collected. Out of 230 participants, 199 (86.5%) were positive for STDs pathogens studied, among them, 98 (42.6%) were infected with single pathogen and 101 (43.9%) were suffering from multiple infections. The prevalences of N. gonorrheae, C. trachomatis, T. pallidum, and HSV type 2 were 90 (39.1%), 110 (47.8%), 28 (12.2%) and 88 (38.2%) respectively. However, none of them were positive for HIV infection. Use of condom was significantly associated with protection of the participants against STDs. Keywords: Sexually Transmitted Diseases, Neisseria gonorrhoeae, Chlamydia trachomatis, Treponema pallidum, Herpes simplex virus type-2, Human Immunodeficiency virus   doi: 10.3329/bjmm.v3i1.2968 Bangladesh J Med Microbiol 2009; 03 (01): 27-33

scholarly journals Effects ofMentha suaveolensEssential Oil onChlamydia trachomatis

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Rosa Sessa ◽  
Marisa Di Pietro ◽  
Fiorenzo De Santis ◽  
Simone Filardo ◽  
Rino Ragno ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Substantial Reduction ◽  
Developmental Cycle ◽  
Elementary Body ◽  
Promising Candidate ◽  
Common Cause ◽  
Sexually Transmitted ◽  
Reticulate Body ◽  
Preventative Strategy

Chlamydia trachomatis, the most common cause of sexually transmitted bacterial infection worldwide, has a unique biphasic developmental cycle alternating between the infectious elementary body and the replicative reticulate body.C. trachomatisis responsible for severe reproductive complications including pelvic inflammatory disease, ectopic pregnancy, and obstructive infertility. The aim of our study was to evaluate whetherMentha suaveolensessential oil (EOMS) can be considered as a promising candidate for preventingC. trachomatisinfection. Specifically, we investigated thein vitroeffects of EOMS towardsC. trachomatisanalysing the different phases of chlamydial developmental cycle. Our results demonstrated that EOMS was effective towardsC. trachomatis, whereby it not only inactivated infectious elementary bodies but also inhibited chlamydial replication. Our study also revealed the effectiveness of EOMS, in combination with erythromycin, towardsC. trachomatiswith a substantial reduction in the minimum effect dose of antibiotic. In conclusion, EOMS treatment may represent a preventative strategy since it may reduceC. trachomatistransmission in the population and, thereby, reduce the number of new chlamydial infections and risk of developing of severe sequelae.

Chlamydia trachomatis infection in women living in remote areas in Amazonas, Brazil—a self-collection screening experience

2018 ◽  
Vol 30 (4) ◽  
pp. 336-343
Author(s):  
Danielle AP Rocha ◽  
Cássia de Oliveira Moraes ◽  
Adriene F Araújo ◽  
Êmille dos Santos Beltrão ◽  
Lainara Castelo dos Santos ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Screening Program ◽  
Consistent Condom ◽  
Consistent Condom Use ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
First Sexual Intercourse ◽  
Organized Screening Program ◽  
Polymerase Chain ◽  
Actual Prevalence

Chlamydia trachomatis infection is the most common sexually transmitted bacterial infection among women. In Brazil, there is no organized screening program for C. trachomatis, and the actual prevalence of infection is unknown. This study aimed to determine the prevalence of C. trachomatis infection in women living in riverside communities in the Amazon, using self-collection employing the Evalyn® Brush and polymerase chain reaction. A total of 299 riverine women aged 18–81 years, mean age 35.7 (±12.8) years, predominantly agricultural workers, with low schooling and living with a partner, participated in this study. The prevalence of C. trachomatis infection was found to be 3.7% (95% CI 1.8–6.5), most of them being symptomatic. The mean age of the first sexual intercourse reported by women was 15.2 (±2.3) years, and the majority reported having had none or only one partner in the last 12 months, with very low adherence to consistent condom use (15.4%). Most women (98.3%) reported having approved using the vaginal self-collecting brush, and only 4.7% reported having difficulty in handling the brush. We consider that a vaginal self-collecting device is adequate for diagnosing C. trachomatis infection in women living in remote, hard-to-reach areas.

scholarly journals Chlamydia trachomatis infection: a challenge for the urologist

2011 ◽  
Vol 2 (1) ◽  
pp. 14 ◽  
Author(s):  
Tommaso Cai ◽  
Sandra Mazzoli ◽  
Nicola Mondaini ◽  
Gianni Malossini ◽  
Riccardo Bartoletti
Keyword(s):  
Chlamydia Trachomatis ◽  
Bacterial Infections ◽  
Reproductive Tract ◽  
Diagnosis And Treatment ◽  
Everyday Clinical Practice ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
New Findings ◽  
Asymptomatic Infections

<p>The role of <em>Chlamydia trachomatis</em> (Ct) in everyday clinical practice is now on the increase because Ct infections are the most prevalent sexually transmitted bacterial infections worldwide. Ct can cause urethritis, cervicitis, pharyngitis, or epididymitis, although asymptomatic infections are quite common. Ct infection remains asymptomatic in approximately 50% of infected men and 70% of infected women, with risk for reproductive tract sequelae both in women and men. A proper early diagnosis and treatment is essential in order to prevent persistent consequences. An accurate comprehension of the pathology, diagnosis and treatment of this entity is essential for the urologist. We review the literature about the new findings in diagnosis and treatment of Ct infection in sexually active young men.</p>

The Gynecologic Health Consequences of Chlamydia trachomatis Infection in Military Servicewomen

2018 ◽  
Vol 36 (06) ◽  
pp. 340-350 ◽  
Author(s):  
Christine Nadeau ◽  
Dennis Fujii ◽  
Jessica Lentscher ◽  
Amanda Haney ◽  
Richard Burney
Keyword(s):  
Chlamydia Trachomatis ◽  
The United States ◽  
Chlamydia Infection ◽  
Tubal Infertility ◽  
Military Health ◽  
Vitro Fertilization ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
The Military

Abstract Chlamydia trachomatis is the most common sexually transmitted bacterial infection in the United States. Within the U.S. military, the age- and race-adjusted chlamydia infection rates among female service members are consistently higher than civilian rates, with a 20% annual acquisition rate among young active-duty women. The sequelae of chlamydia disproportionately impact women in terms of severity and cost. Untreated chlamydia progresses to pelvic inflammatory disease in 40% of cases, and is a leading cause of fallopian tube damage and pelvic adhesive disease resulting in ectopic pregnancy, tubal infertility, and acute and chronic pelvic pain. Tubal infertility is among the leading indications for in vitro fertilization (IVF) nationally and rates among couples undergoing IVF at military treatment centers are double the national average. Collectively, chlamydia infection represents a significant resource burden to the military health care system and, in view of the serious gynecologic health sequelae, a significant threat to the readiness of servicewomen. In this review, we discuss the gynecologic impact of chlamydia infection within the military, the critical gaps for research funding, and opportunities for intervention.

scholarly journals Comparison of Performances of Two Commercially Available Tests, a PCR Assay and a Ligase Chain Reaction Test, in Detection of Urogenital Chlamydia trachomatis Infection

1998 ◽  
Vol 36 (6) ◽  
pp. 1489-1493 ◽  
Author(s):  
Mirja Puolakkainen ◽  
Eija Hiltunen-Back ◽  
Timo Reunala ◽  
Satu Suhonen ◽  
Pekka Lähteenmäki ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Clinical Laboratory ◽  
Transmitted Disease ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Chlamydia Trachomatis Infection ◽  
Sexually Transmitted ◽  
Transport Medium ◽  
Sexually Transmitted Disease Clinic ◽  
Chain Reaction Test

The diagnostic performance of a PCR test (Roche Cobas Amplicor CT/NG Test) and that of a ligase chain reaction (LCR) test (Abbott LCxChlamydia trachomatis assay) were compared by using endocervical and urethral swab specimen culture as a reference test. First-void urine (FVU) and endocervical and urethral swab specimens were collected from 1,015 unselected patients attending a sexually transmitted disease clinic and a clinic for adolescents in Helsinki, Finland. Chlamydia trachomatis was cultured from samples from the endocervix or urethra. PCR was performed with fresh and frozen urine and the culture transport medium. LCR was performed with fresh and frozen urine and LCx swab transport medium. Diagnostic consistency and diagnostic accuracy were statistically tested. The test results were identical for 984 patients (97%). Discrepant results were observed for 31 patients. Overall, LCR and PCR showed excellent kappa coefficients of consistency for both swab and FVU specimens (0.93 and 0.95, respectively). Sixty-one patients (6%) were culture positive. Testing of FVU by LCR or PCR increased the overall positivity rates to 7.0 and 7.7%, respectively. While PCR of FVU detected the greatest number of C. trachomatis infections (sensitivity, 96.1%), for some PCR-positive FVU specimens the results could not be confirmed (specificity, 99.6%). PCR and LCR were more sensitive than culture (sensitivities, 92 and 93% versus 79% for culture) in the diagnosis of genital C. trachomatis infection. In conclusion, both tests can be recommended for use in the clinical laboratory and for the screening of asymptomatic C. trachomatis infections.

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