The effect of Penicillium fungi on plant growth and phosphorus mobilization in neutral to alkaline soils from southern Australia

2007 ◽  
Vol 53 (1) ◽  
pp. 106-115 ◽  
Author(s):  
S A Wakelin ◽  
V V.S.R. Gupta ◽  
P R Harvey ◽  
M H Ryder
Keyword(s):  
Plant Growth ◽  
Dry Matter ◽  
Growth Promotion ◽  
Alkaline Soils ◽  
Plant Seed ◽  
Soil P ◽  
Penicillium Sp ◽  
Extractable P ◽  
Plant P Uptake ◽  
Penicillium Bilaiae

The phosphate solubilizing fungi Penicillium radicum, Penicillium bilaiae (strain RS7B-SD1), and an unidentified Penicillium sp. designated strain KC6-W2 were tested for their ability to increase the growth and phosphorus (P) nutrition of wheat, medic, and lentil in three soils of neutral to alkaline pH reaction. The strongest plant growth promoting (PGP) strain was Penicillium sp. KC6-W2, which stimulated significant increases in shoot growth and dry mass in seven of the nine experiments conducted. Levels of PGP by Penicillium sp. KC6-W2 ranged from 6.6% to 19% and were associated with increased uptake of P to the shoot. The PGP properties of Penicillium sp. KC6-W2 were evident on each of the three different plant species and soil types, a level of reliability not observed in other strains tested. Inoculation of seed with P. radicum increased lentil growth by 5.5% (P < 0.05) in soil from Tarlee but did not affect plant growth in the eight other experiments. Inoculation of plant seed with P. bilaiae RS7B-SD1 resulted in significant PGP in two of the nine experiments conducted. However, when significant, stimulation of PGP by P. bilaiae RS7B-SD1 was strong and resulted in increases in medic dry matter (19%) and lentil shoot dry matter (15%). A soil microcosm experiment investigated the effect of Penicillium fungi on cycling of soil P. Penicillium bilaiae RS7B-SD1 was the only fungus to significantly increase HCO3-extractable P (23% increase; P < 0.05). Production of phosphatase enzymes was not associated with increased HCO3-extractable P. Addition of carbon in the form of ryegrass seed significantly increased microbial respiration and movement of P to the microbial biomass (P < 0.05), but these parameters were irrespective of Penicillium treatment. This work has established the potential for use of Penicillium inoculants to increase plant growth on alkaline soils in Australia. The role of Penicillium fungi in plant P uptake and soil P cycling requires further exploration.Key words: Penicillium, phosphorus, plant growth promotion, phosphatase, rhizobia.

Enhanced tomato plant growth in soil under reduced P supply through microbial inoculants and microbiome shifts

2019 ◽  
Vol 95 (9) ◽  
Author(s):  
Namis Eltlbany ◽  
Mohamed Baklawa ◽  
Guo-Chun Ding ◽  
Dinah Nassal ◽  
Nino Weber ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Growth ◽  
Growth Promotion ◽  
Bacterial Community Composition ◽  
Soil Microbial Communities ◽  
Rrna Gene ◽  
Pseudomonas Sp ◽  
Tomato Plants ◽  
Soil P ◽  
Bacterial Inoculants

ABSTRACT Soil microbial communities interact with roots, affecting plant growth and nutrient acquisition. In the present study, we aimed to decipher the effects of the inoculants Trichoderma harzianum T-22, Pseudomonas sp. DSMZ 13134, Bacillus amyloliquefaciens FZB42 or Pseudomonas sp. RU47 on the rhizosphere microbial community and their beneficial effects on tomato plants grown in moderately low phosphorous soil under greenhouse conditions. We analyzed the plant mass, inoculant colony forming units and rhizosphere communities on 15, 22, 29 and 43 days after sowing. Selective plating showed that the bacterial inoculants had a good rhizocompetence and accelerated shoot and root growth and nutrient accumulation. 16S rRNA gene fingerprints indicated changes in the rhizosphere bacterial community composition. Amplicon sequencing revealed that rhizosphere bacterial communities from plants treated with bacterial inoculants were more similar to each other and distinct from those of the control and the Trichoderma inoculated plants at harvest time, and numerous dynamic taxa were identified. In conclusion, likely both, inoculants and the rhizosphere microbiome shifts, stimulated early plant growth mainly by improved spatial acquisition of available nutrients via root growth promotion. At harvest, all tomato plants were P-deficient, suggesting a limited contribution of inoculants and the microbiome shifts to the solubilization of sparingly soluble soil P.

scholarly journals Evaluation ofStreptomycesspp. for their plant-growth-promotion traits in rice

2013 ◽  
Vol 59 (8) ◽  
pp. 534-539 ◽  
Author(s):  
Subramaniam Gopalakrishnan ◽  
Srinivas Vadlamudi ◽  
Shravya Apparla ◽  
Prakash Bandikinda ◽  
Rajendran Vijayabharathi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Dry Matter ◽  
Microbial Biomass Carbon ◽  
Growth Promotion ◽  
Dry Weight ◽  
Available Phosphorus ◽  
Carbon And Nitrogen ◽  
Highly Sensitive ◽  
Pgp Activities

Five strains of Streptomyces (CAI-17, CAI-68, CAI-78, KAI-26, and KAI-27) were previously reported to have potential for charcoal rot control and plant growth promotion (PGP) in sorghum. In this study, those 5 Streptomyces strains were characterized for their enzymatic activities and evaluated for their PGP capabilities on rice. All the Streptomyces strains were able to produce lipase and β-1,3-glucanase; grew in NaCl (up to 8%), at pH 5–13, and at temperatures 20–40 °C; and were resistant to ampicillin, sensitive to nalidixic acid, and highly sensitive to chloramphenicol, kanamycin, streptomycin, and tetracycline. They were highly tolerant to the fungicide bavistin but were highly sensitive to benlate, benomyl, and radonil. When evaluated on rice in the field, Streptomyces significantly enhanced tiller and panicle numbers, stover and grain yields, dry matter, root length, volume and dry weight, compared with the control. In the rhizosphere at harvest, microbial biomass carbon and nitrogen, dehydrogenase activity, total nitrogen, available phosphorus, and % organic carbon were also found significantly higher in Streptomyces-treated plots than in the control plots. This study further confirms that the selected Streptomyces have PGP activities.

scholarly journals Growth-Promoting Potential of Rhizobacteria Isolated From Sugarcane

2021 ◽  
Vol 5 ◽  
Author(s):  
Roberta Mendes dos Santos ◽  
Everlon Cid Rigobelo
Keyword(s):  
Plant Growth ◽  
Dry Matter ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Ribosomal Gene ◽  
Agricultural Crop ◽  
Cellulolytic Activity ◽  
Bacterial Strains ◽  
Crop Species ◽  
Growth Promoting

The search for plant growth-promoting rhizobacteria (PGPRs) addresses the ongoing need for new bioinoculants to be used on various agricultural crop species, including sugarcane. Bacterial strains were isolated from the rhizosphere of sugarcane plants and identified by sequencing the 16S ribosomal gene. The main indole acetic acid producers were Enterobacter sp. IP11, Enterobacter sp. IP14, and E. asburiae IP24. Achromobacter spanius IP23 presented the highest levels of cellulolytic activity and potassium solubilization. Bacillus thuringiensis IP21 and Staphylococcus saprophyticus IJ8 showed the highest levels of fixed nitrogen. The levels of calcium phosphate and aluminum phosphate in B. thuringiensis IP21 were notable, as this strain solubilized 481.00 and 39.33 mg of phosphorus mL−1, respectively; however, for Araxá apatite, the results for B. anthracis IP17 were notable (622.99 mg phosphorus mL−1), while for iron phosphate solubilization, Enterobacter sp. IP14, which solubilized 105.66 mg phosphorus mL−1 was notable. The B. thuringiensis IP21 and Enterobacter sp. IP11 isolates promoted the growth of the tallest sugarcane plants, inducing increases of 14.1 and 10.4% relative to the control plants, respectively. For shoot dry matter, root dry matter, and total dry matter, plants inoculated with Enterobacter sp. IP14, B. anthracis IP17, and A. spanius IP23 presented higher values than the controls. Furthermore, plants inoculated with B. thuringiensis IP21 presented higher root dry matter and total dry matter values, and those inoculated with Enterobacter sp. IP14 also presented higher total dry matter values. These results indicate that bacteria with the potential for use as future inoculants should be investigated since bacteria with plant growth-related characteristics may not impact growth promotion.

scholarly journals Bacterial Formulations in Induction of Resistance and Growth Promotion of Tomato Plants

2018 ◽  
Vol 10 (10) ◽  
pp. 493
Author(s):  
José R. M. Campos Neto ◽  
Rafael Ribeiro Chaves ◽  
Diogo Herison Silva Sardinha ◽  
Luiz Gustavo de Lima Melo ◽  
Antônia Alice Costa Rodrigues
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Seed Treatment ◽  
Dry Matter ◽  
Growth Promotion ◽  
Systemic Resistance ◽  
Tomato Plants ◽  
Vigor Index ◽  
Promote Plant Growth ◽  
Induction Of Resistance

The objective of this work was to evaluate the effectiveness of seed treatment with fresh suspensions and powder formulations with Bacillus methylotrophicus to promote plant growth and induction of resistance against fusarium wilt (Fusarium oxysporum f. sp. lycopersici) in tomato plants under greenhouse conditions, verifying the occurrence of morphological and biochemical changes in the evaluated plants. Powder formulations based on Cassava (Manihot esculenta), Arrowroot (Maranta arundinacea) and sodium alginate containing Bacillus, in addition to the commercial product Quartz&reg;, were used to microbiolize the tomato seeds of the cultivar Santa Cruz. The formulations promoted plant growth, with a seedling vigor index greater than 50% for all treatments containing B. mthylotrophicus, in addition to a significant increase in total dry matter. The treatments induced systemic resistance, controlling the fusarium wilt with a 75% reduction of the disease and activation of enzymes such as peroxidase and polyphenoloxidase, only &beta;-1,3-glucanase presented less activity than controls (treatments without B. mthylotrophicus). Thus, the use of formulations containing Bacillus are efficient in promoting plant growth of tomato plants and in inducing resistance to the control of fusarium wilt.

scholarly journals Four soil phosphorus (P) tests evaluated by plant P uptake and P balancing in the Ultuna long-term field experiment

2018 ◽  
Vol 64 (No. 9) ◽  
pp. 441-447 ◽  
Author(s):  
Jarosch Klaus A ◽  
Santner Jakob ◽  
Parvage Mohammed Masud ◽  
Gerzabek Martin Hubert ◽  
Zehetner Franz ◽  
...  
Keyword(s):  
Soil Phosphorus ◽  
P Uptake ◽  
P Availability ◽  
Soil P ◽  
Extractable P ◽  
Plant P Uptake ◽  
Soil P Tests ◽  
Water Extractable ◽  
Term Field

Soil phosphorus (P) availability was assessed with four different soil P tests on seven soils of the Ultuna long-term field experiment (Sweden). These four soil P tests were (1) P-H<sub>2</sub>O (water extractable P); (2) P-H<sub>2</sub>O<sub>C10</sub> (water extractable P upon 10 consecutive extractions); (3) P-AL (ammonium lactate extractable P) and (4) P-C<sub>DGT</sub> (P desorbable using diffusive gradients in thin films). The suitability of these soil P tests to predict P availability was assessed by correlation with plant P uptake (mean of preceding 11 years) and soil P balancing (input vs. output on plot level for a period of 54 years). The ability to predict these parameters was in the order P-H<sub>2</sub>O<sub>C10</sub> &gt; P-C<sub>DGT</sub> &gt; P-H<sub>2</sub>O &gt; P-AL. Thus, methods considering the P-resupply from the soil solid phase to soil solution performed clearly better than equilibrium-based extractions. Our findings suggest that the P-AL test, commonly used for P-fertilizer recommendations in Sweden, could not predict plant P uptake and the soil P balance in a satisfying way in the analysed soils.

scholarly journals Effects of biotite syenite on the nutrient levels and electrical charges in a Brazilian Savanna Ferralsol

2021 ◽  
Vol 51 ◽  
Author(s):  
Luiz Fernando dos Santos ◽  
Fernando Fabriz Sodré ◽  
Éder de Souza Martins ◽  
Cícero Célio de Figueiredo ◽  
Jader Galba Busato
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Environmental Impacts ◽  
Dry Matter ◽  
Brazilian Savanna ◽  
Natural Fertility ◽  
Alternative Source ◽  
Soil P ◽  
Maize Leaves ◽  
Maize Plants

ABSTRACT In the Brazilian Savanna, most of the soils have a low natural fertility. In order to overcome this limitation, farmers use large amounts of soluble fertilizers, causing environmental impacts. In the present study, finely ground biotite syenite (0; 600; 1,200; 1,800; and 2,400 kg ha-1) was evaluated as an alternative source of nutrients and electrical charges in a weathered Ferralsol from the Brazilian Savanna. Additionally, the development and nutrition of maize plants were analyzed. The values of Ca + Mg, T, t, Zn, Cu, Fe and Mn in the soil were not affected by the addition of biotite syenite, but the available P and K and the permanent charges were increased. The biotite did not increase the contents of P, Ca and Cu in the maize leaves, but it increased the contents of N and K. Increases of 103, 125 and 128 % were also detected, respectively, for plant height, stem diameter and leaf dry matter. The biotite syenite was able to increase the soil P, K and permanent charges, as well as the maize uptake of N and K, thus helping in the plant growth.

scholarly journals A Dual Isotope Protocol to Separate the Contributions to Phosphorus Content of Maize Shoots and Soil Phosphorus Fractions from Biosolids, Fertilizer and Soil

2018 ◽  
Vol 6 (3) ◽  
pp. 236-242
Author(s):  
JEAN DAVIS ◽  
RICHARD J FLAVEL ◽  
GRAEME BLAIR
Keyword(s):  
Soil Phosphorus ◽  
Application Rate ◽  
P Uptake ◽  
Soil P ◽  
Dual Isotope ◽  
Extractable P ◽  
Plant P Uptake ◽  
The Difference ◽  
Biosolids Application ◽  
Total P

Separation of the phosphorus (P) contributions from soil, fertilizer and biosolids to plants has not been possible without the aid of radioisotopes. Dual labelling of soil with 32P and fertilizer with 33P isotopes has been used to partition the sources of P in maize (Zea mays) shoots and in soil P pools. Biosolids containing 4.1% P that had been prepared using Fe and Al were applied to a Kurosol soil from Goulburn, NSW, Australia. The biosolids were applied at five rates up to 60 dry t/ha with and without P fertilizer. Phosphorus derived from fertilizer was determined directly with33 P and that from soil by32 P reverse dilution. Phosphorus derived from biosolids was estimated as the difference between total P and that derived from the soil plus fertilizer calculated from isotope data. Yield and P content of maize shoots increased linearly with the rate of biosolidsapplication. The proportion of P in the plant derived from biosolids also increased with application rate up to 88% for the soil receiving biosolids at 60 dry t/ha with no fertilizer. The corresponding value with fertilizer applied at 80 kg P/ha was 69%. The proportion of P in the maize shoots derived from soil and fertilizer decreased as biosolids application rate increased. Soil total P, bicarbonate extractable P, Al-P, Fe-P and Ca-P increased with biosolids application rate. The increase in plant P uptake and in bicarbonate extractable P in the soil shows that biosolids P provides a readily available source of P. A decrease in uptake of fertilizer and soil P with increasing biosolids application is attributed to the decrease in the proportion of P from these sources in the total pool of available P, rather than to immobilization of P by Fe and Al in the biosolids.

Predicting the response of wheat (Triticum aestivum L.) to liquid and granular phosphorus fertilisers in Australian soils

Soil Research ◽  
2007 ◽  
Vol 45 (6) ◽  
pp. 448 ◽  
Author(s):  
T. M. McBeath ◽  
M. J. McLaughlin ◽  
R. D. Armstrong ◽  
M. Bell ◽  
M. D. A. Bolland ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Calcium Carbonate ◽  
Dry Matter ◽  
Calcareous Soils ◽  
Triticum Aestivum L ◽  
Soil Test ◽  
Alkaline Soils ◽  
Test Procedures ◽  
Soil P ◽  
Yield Responses

Liquid forms of phosphorus (P) have been shown to be more effective than granular P for promoting cereal growth in alkaline soils with high levels of free calcium carbonate on Eyre Peninsula, South Australia. However, the advantage of liquid over granular P forms of fertiliser has not been fully investigated across the wide range of soils used for grain production in Australia. A glasshouse pot experiment tested if liquid P fertilisers were more effective for growing spring wheat (Triticum aestivum L.) than granular P (monoammonium phosphate) in 28 soils from all over Australia with soil pH (H2O) ranging from 5.2 to 8.9. Application of liquid P resulted in greater shoot biomass, as measured after 4 weeks’ growth (mid to late tillering, Feeks growth stage 2–3), than granular P in 3 of the acidic to neutral soils and in 3 alkaline soils. Shoot dry matter responses of spring wheat to applied liquid or granular P were related to soil properties to determine if any of the properties predicted superior yield responses to liquid P. The calcium carbonate content of soil was the only soil property that significantly contributed to predicting when liquid P was more effective than granular P. Five soil P test procedures (Bray, Colwell, resin, isotopically exchangeable P, and diffusive gradients in thin films (DGT)) were assessed to determine their ability to measure soil test P on subsamples of soil collected before the experiment started. These soil test values were then related to the dry matter shoot yields to assess their ability to predict wheat yield responses to P applied as liquid or granular P. All 5 soil test procedures provided a reasonable prediction of dry matter responses to applied P as either liquid or granular P, with the resin P test having a slightly greater predictive capacity on the range of soils tested. The findings of this investigation suggest that liquid P fertilisers do have some potential applications in non-calcareous soils and confirm current recommendations for use of liquid P fertiliser to grow cereal crops in highly calcareous soils. Soil P testing procedures require local calibration for response to the P source that is going to be used to amend P deficiency.

scholarly journals Co-inoculation With Tropical Strains of Azospirillum and Bacillus Is More Efficient Than Single Inoculation for Improving Plant Growth and Nutrient Uptake in Maize.

2021 ◽  
Author(s):  
Vitoria Palhares Ribeiro ◽  
Eliane Aparecida Gomes ◽  
Sylvia Morais de Sousa ◽  
Ubiraci Gomes de Paula Lana ◽  
Antonio Marcos Coelho ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Plant Growth ◽  
Surface Area ◽  
Growth Promotion ◽  
Cropping Systems ◽  
Dry Weight ◽  
Total Surface Area ◽  
Soil P ◽  
Maize Growth ◽  
Root Dry Weight

Abstract Usage of Bacillus and Azospirillum as new eco-friendly microbial consortium inoculants is a strategy to increase plant growth and crop yield by improving nutrient availability in agricultural systems. In this study, we designed a multispecies inoculum containing B. thuringiensis (strain B116), B. subtillis (strain B2084) and Azospirillum brasilense (strains A1626 and A2142) to investigate their individual or co-inoculated ability to solubilize and mineralize phosphate, produce indole acetic acid (IAA) and their effect on maize growth promotion in hydroponics and in a non-sterile soil. All strains showed significant IAA production, P mineralization (sodium phytate) and Ca-P, Fe-P (tricalcium phosphate and iron phosphate, respectively) solubilization. In hydroponics, co-inoculation with A1626 x A2142, B2084 x A2142, B2084 x A1626 resulted in higher root total length, total surface area, and surface area of roots with diameter between 0 and 1 mm than other treatments with single inoculant, except B2084. In a greenhouse experiment, maize inoculated with the two Azospirillum strains exhibited enhanced shoot dry weight, shoot P and K content, root dry weight, root N and K content and acid and alkaline phosphatase activities than the other treatments. There was a significant correlation between soil P and P shoot, alkaline phosphatase and P shoot and between acid phosphatase and root dry weight. It may be concluded that co-inoculations are most effective than single inoculants strains, mainly between two selected Azospirillum strains. Thus, they could have synergistic interactions during maize growth, and be useful in the formulation of inoculants to improve the cropping systems sustainable.

scholarly journals Tree seedling growth promotion by dual inoculation with Rhizoglomus fasciculatum (Thaxt.) Sieverding, Silva & Oehl and Mortierella sp., rhizosphere fungi for reforestation purposes, to promote plant P uptake and growth at the nursery state

2016 ◽  
Vol 65 (3) ◽  
pp. 239-247 ◽  
Author(s):  
Josaly Moreno ◽  
Juan Diego León ◽  
Nelson Walter Osorio
Keyword(s):  
Plant Growth ◽  
Plant Species ◽  
Seedling Growth ◽  
Growth Promotion ◽  
Mycorrhizal Fungus ◽  
P Uptake ◽  
Plant Performance ◽  
P Deficiency ◽  
Plant P Uptake ◽  
Dual Inoculation

<p>One of the most promising techniques to promote seedling growth of tree species at nursery is the use of rhizosphere microorganisms. This is particularly relevant in enhancing plant performance in reforestation of degraded lands. A series of experiments were conducted to evaluate the effectiveness of either individual inoculation with the arbuscular mycorrhizal fungus Rhizoglomus fasciculatum (Thaxt.) Sieverding, Silva &amp; Oehl or dual inoculation with R. fasciculatum and the phosphate solubilizing fungus Mortierella sp., at two doses (50 and 100 kg of inoculum per m3 of substrate), on plant growth and phosphorus (P) uptake of Tecoma stans, Dodonaea viscosa, Fraxinus chinensis, and Lafoensia speciosa. These plant species are commonly used in Colombia in reforestation programs. The results indicated that treatments were effective to increase plant performance of the four plant species; however, the effect was significantly higher when both fungi R. fasciculatum and Mortierella sp. were concomitantly inoculated than when only the mycorrhizal fungus was applied. Overall, the dual inoculation at the dose of 50 kg m-3 had higher or similar effects than at 100 kg m-3. The results suggest that the dual inoculation was more effective in order to prevent plant P deficiency and stimulate plant growth.</p>

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