Exploration of members ofAspergillussectionsNigri,Flavi, andTerreifor feruloyl esterase production

2006 ◽  
Vol 52 (9) ◽  
pp. 886-892 ◽  
Author(s):  
Ourdia Bouzid ◽  
Eric Record ◽  
Michèle Asther ◽  
Mireille Haon ◽  
David Navarro ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Methyl Esters ◽  
Type A ◽  
Sugar Beet Pulp ◽  
Feruloyl Esterase ◽  
Type B ◽  
Original Activity ◽  
Beet Pulp ◽  
Feruloyl Esterases ◽  
Oat Spelt

The ability of members of Aspergillus sections Nigri, Flavi, and Terrei to produce feruloyl esterases was studied according to their substrate specificity against synthetic methyl esters of hydroxycinnamic acids. Type A feruloyl esterases (FAEA), induced during growth on cereal-derived products, show a preference for the phenolic moiety of substrates that contain methoxy substitutions, as found in methyl sinapinate, whereas type B feruloyl esterases (FAEB) show a preference for the phenolic moiety of substrates that contain hydroxyl substitutions, as occurs in methyl caffeate. All the strains of Aspergillus section Nigri (e.g., A. niger and A. foetidus) were able to produce feruloyl esterases with activity profiles similar to those reported for FAEA and FAEB of A. niger when grown on oat–spelt xylan and sugar beet pulp, respectively. The two genes encoding these proteins, faeA and faeB, were identified by Southern blot analysis. The strains of Aspergillus sections Flavi (e.g., A. flavus, A. flavo-furcatus, and A. tamarii) and Terrei (e.g., A. terreus) were able to produce type A and type B enzymes. faeA was revealed in genomic DNA of these strains, and FAEA was determined by immunodetection in cultures grown in oat–spelt xylan. In addition, type B enzymes, not related to faeB, were efficiently induced by oat–spelt xylan and exhibited very original activity profiles on sugar beet pulp. This work confirms that the members of the genus Aspergillus are good feruloyl esterase producers.Key words: Aspergillus, Nigri, Flavi, Terrei, feruloyl esterase.

scholarly journals A non-modular type B feruloyl esterase from Neurospora crassa exhibits concentration-dependent substrate inhibition

2003 ◽  
Vol 370 (2) ◽  
pp. 417-427 ◽  
Author(s):  
Valerie F. CREPIN ◽  
Craig B. FAULDS ◽  
Ian F. CONNERTON
Keyword(s):  
Cell Wall ◽  
Sugar Beet ◽  
Ferulic Acid ◽  
Neurospora Crassa ◽  
Plant Cell Wall ◽  
Substrate Inhibition ◽  
Type B ◽  
Ferulic Acid Esterase ◽  
Beet Pulp ◽  
Feruloyl Esterases

Feruloyl esterases, a subclass of the carboxylic acid esterases (EC 3.1.1.1), are able to hydrolyse the ester bond between the hydroxycinnamic acids and sugars present in the plant cell wall. The enzymes have been classified as type A or type B, based on their substrate specificity for aromatic moieties. We show that Neurospora crassa has the ability to produce multiple ferulic acid esterase activities depending upon the length of fermentation with either sugar beet pulp or wheat bran substrates. A gene identified on the basis of its expression on sugar beet pulp has been cloned and overexpressed in Pichia pastoris. The gene encodes a single-domain ferulic acid esterase, which represents the first report of a non-modular type B enzyme (fae-1 gene; GenBank accession no. AJ293029). The purified recombinant protein has been shown to exhibit concentration-dependent substrate inhibition (Km 0.048mM, Ki 2.5mM and Vmax 8.2units/mg against methyl 3,4-dihydroxycinnamate). The kinetic behaviour of the non-modular enzyme is discussed in terms of the diversity in the roles of the feruloyl esterases in the mobilization of plant cell wall materials and their respective modes of action.

Obtaining of sorbing material based on sugar beet pulp

2019 ◽  
pp. 10-16
Author(s):  
S.V. Meshcheryakov ◽  
◽  
I.S. Eremin ◽  
D.O. Sidorenko ◽  
M.S. Kotelev ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Sugar Beet Pulp ◽  
Beet Pulp

Kinetic and thermodynamic study of the adsorption of calcium onto sugar beet pulp

2016 ◽  
pp. 565-570
Author(s):  
Huang Qin ◽  
Zhu Si-ming ◽  
Zeng Di ◽  
Yu Shu-juan
Keyword(s):  
Sugar Beet ◽  
Adsorption Equilibrium ◽  
Ph Value ◽  
Hard Water ◽  
Sugar Beet Pulp ◽  
Order Kinetic ◽  
Factors Affecting ◽  
Isotherm Equation ◽  
Equilibrium Data ◽  
Beet Pulp

Sugar beet pulp (SBP) was used as low value adsorbent for the removal of calcium from hard water. Batch experiments were conducted to determine the factors affecting adsorption of the process such as pH value and Ca concentration. The adsorption equilibrium of Ca2+ by the SBP is reached after 100min and a pseudo second-order kinetic model can describe the adsorption process. The initial concentrations of Ca varied from 927 to 1127mgCa2+/L. A dose of 30g/L sugar beet pulp was sufficient for the optimum removal of calcium. The overall uptake of Ca ions by sugar beet pulp has its maximum at pH=8. The adsorption equilibrium data fitted well with the Langmuir adsorption isotherm equation.

Possibilities of joint treatment of spent sugar beet pulp and waste water from a sugar factory

2012 ◽  
pp. 756-761 ◽  
Author(s):  
Miroslav Hutnan ◽  
Štefan Tóth ◽  
Igor Bodík ◽  
Nina Kolesárová ◽  
Michal Lazor ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Sugar Beet ◽  
Pilot Plant ◽  
Biogas Production ◽  
Sugar Beet Pulp ◽  
Spare Capacity ◽  
Anaerobic Reactor ◽  
Sugar Factory ◽  
Beet Pulp ◽  
Joint Treatment

The possibility of joint treatment of spent sugar beet pulp and wastewater from a sugar factory was studied in this work. Works focused on processing of spent sugar beet pulp separately or together with other substrates can be found in the literature. In the case of some sugar factories, which have spare capacity in the anaerobic reactor on an anaerobic-aerobic wastewater treatment plant, joint processing of spent sugar beet pulp and wastewater from the sugar factory might be an interesting option. The results of the operation of a pilot plant of an anaerobic reactor with a capacity of 3.5 m3 are discussed. Operation of the pilot plant confirmed the possibility of cofermentation of these materials. The organic loading rate achieved in the anaerobic reactor was higher than 6 kg/(m3·d) (COD), while more than half of the load was provided by spent sugar beet pulp. The addition of sugar beet pulp decreased the concentration of ammonia nitrogen in the anaerobic reactor and it was even necessary to add nitrogen. However, the nitrogen content in sludge water depends on the C:N ratio in the processed sugar beet pulp, therefore this knowledge cannot be generalized. About 1.5 to 2-fold biogas production can be expected from the cofermentation of wastewater with sugar beet pulp in an anaerobic reactor, compared with the biogas production from just wastewater treatment.

Pomelo peel and sugar beet pulp as novel biosorbents in purification of biodiesel

Biofuels ◽  
2021 ◽  
pp. 1-8
Author(s):  
Saida Ibragić ◽  
Narcisa Smječanin ◽  
Ranko Milušić ◽  
Mirza Nuhanović
Keyword(s):  
Sugar Beet ◽  
Sugar Beet Pulp ◽  
Pomelo Peel ◽  
Beet Pulp

scholarly journals The Influence of Extruded Sugar Beet Pulp on Cookies' Nutritional, Physical and Sensory Characteristics

2021 ◽  
Vol 13 (9) ◽  
pp. 5317
Author(s):  
Sonja Simić ◽  
Jovana Petrović ◽  
Dušan Rakić ◽  
Biljana Pajin ◽  
Ivana Lončarević ◽  
...  
Keyword(s):  
Particle Size ◽  
Sugar Beet ◽  
Dietary Fiber ◽  
Wheat Flour ◽  
Nutritional Quality ◽  
Sugar Beet Pulp ◽  
Sensory Characteristics ◽  
Total Dietary Fiber ◽  
Beet Pulp

Sugar beet pulp (SBP) is a by-product of the sugar industry in which the dietary fiber content ranges from 73% to 80%. Compared to cereal fibers mainly used in biscuit production, sugar beet fibers are gluten free and have a perfect ratio of 2/3 insoluble fiber. In this work, sugar beet pulp was extruded with corn grits (ratios of corn grits to sugar beet pulp in extrudates were 85:15, 70:30, and 55:45), and the obtained sugar beet pulp extrudates (SBPEs) were used for improving the nutritional quality of cookies. The wheat flour in cookies was replaced with SBPEs in the amount of 5, 10, and 15%. The influence of three factors (the percentage of sugar beet pulp in the SBPEs, the size of the SBPE particles, and the percentage of wheat flour substituted with SBPEs) and their interactions on the nutritional quality of cookies, as well as their physical and sensory characteristics are examined using the Box–Behnken experimental design. The addition of extruded sugar beet pulp (SBPEs) significantly increased the amount of total dietary fiber and mineral matter of cookies. On the whole, the addition of SBPEs increased cookie hardness, but the hardness decreased with an increase in extrudate particle size. Sensory characteristics (except for the taste) were the most influenced by extrudate particle size.

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