Response surface methodology for the optimization of keratinase production in culture medium containing feathers produced by Kocuria rosea

2006 ◽  
Vol 52 (5) ◽  
pp. 445-450 ◽  
Author(s):  
C Bernal ◽  
I Diaz ◽  
N Coello
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Batch Fermentation ◽  
Culture Medium ◽  
Medium Optimization ◽  
Fold Increase ◽  
Medium Composition ◽  
Central Composite Experimental Design ◽  
Kocuria Rosea ◽  
Central Composite

A 43-fold increase in keratinase production by Kocuria rosea was achieved in batch fermentation using response surface methodology. Factorial designs were used to select the components of a culture medium that showed a significant effect on keratinase production. An orthogonal–central composite experimental design was performed, with only two (feathers and magnesium) from nine initial compounds being further analyzed by response surface methodology. An optimum keratinase production of 14 886.9 U/mg was obtained with the following medium composition (per litre): NH4Cl, 0.3 g; NaCl, 0.3 g; K2HPO4, 3.2 g; KH2PO4, 4.0 g; MgSO4·6H2O, 0.5 g; yeast extract, 0.1 g; and finely milled feathers, 30 g. The medium was shaken at 400 r/min with an incubation period of 14 h at 40 °C.Key words: feathers, keratinases, Kocuria rosea, medium optimization, response surface methodology.

scholarly journals Chitinase from a Novel Strain ofSerratia marcescensJPP1 for Biocontrol of Aflatoxin: Molecular Characterization and Production Optimization Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Kai Wang ◽  
Pei-sheng Yan ◽  
Li-xin Cao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Pcr Amplification ◽  
Fold Increase ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Production Optimization ◽  
Reading Frame ◽  
Mycolytic Enzymes ◽  
Burman Design

Chitinase is one of the most important mycolytic enzymes with industrial significance, and produced by a number of organisms. A chitinase producing isolateSerratia marcescensJPP1 was obtained from peanut hulls in Jiangsu Province, China, and exhibited antagonistic activity against aflatoxins. In this study, we describe the optimization of medium composition with increased production of chitinase for the selected bacteria using statistical methods: Plackett-Burman design was applied to find the key ingredients, and central composite design of response surface methodology was used to optimize the levels of key ingredients for the best yield of chitinase. Maximum chitinase production was predicted to be 23.09 U/mL for a 2.1-fold increase in medium containing 12.70 g/L colloidal chitin, 7.34 g/L glucose, 5.00 g/L peptone, 1.32 g/L (NH4)2SO4, 0.7 g/L K2HPO4, and 0.5 g/L MgSO4·7H2O. Polymerase chain reaction (PCR) amplification of the JPP1 chitinase gene was performed and obtained a 1,789 bp nucleotide sequence; its open reading frame encoded a protein of 499 amino acids named as ChiBjp.

scholarly journals Application of Response Surface Methodology for Optimizing Arginine Deiminase Production Medium forEnterococcus faeciumsp. GR7

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Baljinder Kaur ◽  
Rajinder Kaur
Keyword(s):  
Response Surface Methodology ◽  
Central Composite Design ◽  
Response Surface ◽  
Specific Activity ◽  
Basal Medium ◽  
Fold Increase ◽  
Arginine Deiminase ◽  
Arginine Metabolism ◽  
Central Composite ◽  
Fermentation Media

Arginine metabolism inEnterococcus faeciumsp. GR7 was enhanced via arginine deiminase pathway. Process parameters including fermentation media and environmental conditions were optimized using independent experiments and response surface methodology (central composite design). Fermentation media (EAPM) were optimized using independent experiments which resulted in 4-fold increase in arginine deiminase specific activity as compared to basal medium. To further enhance arginine deiminase activity inE. faeciumsp. GR7 and biomass production including a five-level central composite design (CCD) was employed to study the interactive effect of three-process variables. Response surface methodology suggested a quadratic model which was further validated experimentally where it showed approximately 15-fold increase in arginine metabolism (in terms of arginine deiminase specific activity) over basal medium. By solving the regression equation and analyzing the response surface cartons, optimal concentrations of the media components (g/L) were determined as arginine 20.0; tryptone 15.0; lactose 10.0; K2HPO43.0; NaCl 1.0, MnSO40.6 mM; Tween 80 1%; pH 6.0 for achieving specific arginine deiminase activity of 4.6 IU/mG with concomitant biomass production of 12.1 mg/L. The model is significant as the coefficient of determination (R2) was 0.87 to 0.90 for all responses. Enhanced arginine deiminase yield fromE. faecium, a GRAS lactic acid bacterial strain, is desirable to explorein vitrotherapeutic potential of the arginine metabolizingE. faeciumsp. GR7.

scholarly journals Statistical optimization of culture medium composition for enhanced zeaxanthin production by Cyanophycean microalgae Trichodesmium thiebautii (NIOT 152)

2020 ◽  
Vol 13 (41) ◽  
pp. 4307-4318
Author(s):  
Priyanka Srinivasan ◽  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sea Water ◽  
Fold Increase ◽  
Medium Composition ◽  
Statistical Optimization ◽  
Age Related Macular Degeneration ◽  
Medium Components ◽  
Age Related ◽  
Trichodesmium Thiebautii

Background/Objectives: Zeaxanthin is a xanthophyll carotenoid revered for its role in the prevention of age related macular degeneration. The study evaluated the zeaxanthin accumulation of the marine Cyanophycean alga Trichodesmium thiebautii (NIOT 152). A sequential statistical technique was applied to optimize the Artificial Sea Water nutrient medium (ASN-III) components for enhancing the zeaxanthin accumulation in T. thiebautii. Methods: A two-level statistical approach involving Plackett-Burman (PB) design to screen the most important nutrients influencing the zeaxanthin accumulation followed by Response surface methodology (RSM) was employed. The results of PB design revealed sodium nitrate, disodium EDTA, magnesium sulphate and sodium carbonate as the crucial medium components for increasing zeaxanthin accumulation. Further, RSM was employed to study the interaction between these factors and identified an optimum concentration of the ingredients for higher zeaxanthin production. Findings: The optimized medium components resulted in 2.33 fold increase in zeaxanthin accumulation (4.3 ± 1.29 mg L-1) as compared to ASN III medium (1.84 ± 0.12 mg L-1). Novelty: There are only few studies on laboratory cultured Trichodesmium and only very few reports are available regarding pigment production from Trichodesmium sp. The present study successfully demonstrated the statistical optimization of ASN III medium to improve zeaxanthin accumulation by Trichodesmium thiebautii. Keywords: ASN III medium; zeaxanthin; Trichodesmium thiebautii; Plackett-Burman; response surface methodology REFERENCE

Research on Blanching Pretreatment of Quick Frozen Sword Bean (Canavalia gladiate)

2011 ◽  
Vol 140 ◽  
pp. 416-420
Author(s):  
Chen Wei Zhou ◽  
Juan Xu ◽  
Qing Qing Li ◽  
Rui Zhi Wang ◽  
Lu Yang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Statistical Model ◽  
Response Surface ◽  
Peroxidase Activity ◽  
Primary Determinant ◽  
Central Composite Experimental Design ◽  
Main Index ◽  
Temperature Time ◽  
Central Composite

Quick frozen could preserve the maximum micro-texture and taste quality of vegetables, and blanching pretreatment is primary determinant of quick frozen. In this study, the central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of blanching pretreatment on the quality of sword bean (Canavalia gladiate), with peroxidase activity as main index. The pretreatment process optimized with response surface methodology was blanching at 96 °C for 1.03 min. Through optimization, a statistical model was established for the relation between blanching temperature, time and peroxidase activity, which would facilitate the prediction of the effects of blanching on the quality of sword bean and could play a guidance role in the blanching pretreatment of many other vegetables.

scholarly journals Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology

2017 ◽  
Vol 26 (1) ◽  
pp. 57-64
Author(s):  
Abd-El Aziem Farouk ◽  
Anis Shobirin Meor Hussin ◽  
Ralf Greiner ◽  
Shareef Mohideen Ismail ◽  
Hamadah Mohd Nur Lubis
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Temperature ◽  
Expression System ◽  
Optimal Conditions ◽  
Phytase Production ◽  
E Coli ◽  
Central Composite Experimental Design ◽  
Full Factorial ◽  
Central Composite

The production of phytase in a recombinant E.coli using the pBAD expression  system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3  central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature.  Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.   

scholarly journals Statistical Optimization of the Medium Composition by Response Surface Methodology to Enhance Schizophyllan Production by Schizophyllum commune

2011 ◽  
Vol 66 (3-4) ◽  
pp. 173-181 ◽  
Author(s):  
Wenbing Li ◽  
Pengpeng Zhou ◽  
Longjiang Yu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Schizophyllum Commune ◽  
Fermentation Medium ◽  
Medium Composition ◽  
Excellent Correlation ◽  
Predicted Values ◽  
Optimal Medium ◽  
Central Composite

The response surface methodology (RSM) involving central composite design (CCD) was employed to optimize the fermentation medium for the cell growth and schizophllan production by Schizophyllum commune CGMCC 5.113 in submerged culture at pH 6.5 and 26°C. The four variables involved in this study were glucose, yeast extract, ammonium nitrate, and magnesium sulfate. The statistical analysis of the results showed that, in the range studied, glucose and yeast extract had a highly significant effect on schizophyllan production. The optimal medium for schizophyllan production calculated from the regression model of RSM was as follows: glucose, 18 g/l; yeast extract, 0.5 g/l; NH4NO3, 0.48 g/l; and MgSO4, 0.05 g/l, with a predicted maximum schizophyllan production of 11.74 g/l. These predicted values were experimentally validated. The excellent correlation between predicted and measured values justifies the validity of the response model. The results of bioreactor fermentation also show that the optimized medium enhanced schizophyllan production (12.80 g/l) by S. commune in a 5-l fermenter

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