Occurrence of parasporin-producingBacillus thuringiensisin Vietnam

2006 ◽  
Vol 52 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Koichi Yasutake ◽  
Ngo Dinh Binh ◽  
Kumiko Kagoshima ◽  
Akiko Uemori ◽  
Akira Ohgushi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cell Killing ◽  
Nucleotide Sequence Analysis ◽  
Human Cancer Cells ◽  
Soil Isolate ◽  
Mosquitocidal Activity ◽  
Anti Cancer ◽  
Larvicidal Activities

A total of 63 Bacillus thuringiensis isolates were recovered from urban soils of Hanoi, Vietnam. Of these, 34 were identified to 12 H serogroups. None of the isolates showed larvicidal activities against three lepidopterous insects. Three isolates belonging to the two serovars, colmeri (H21) and konkukian (H34), were highly toxic to larvae of the mosquito Aedes aegypti. Parasporal inclusion proteins of four isolates exhibited cytocidal activities against HeLa cells. Immunologically, proteins of four isolates were closely related to parasporin-1 (Cry31Aa), a parasporal protein that preferentially kills human cancer cells. Haemolytic activities were associated with parasporal proteins of the three mosquitocidal isolates but not with those of the four cancer-cell-killing isolates. PCR experiments and nucleotide sequence analysis revealed that the genes of four anti-cancer isolates are closely related to the gene parasporin-1 (cry31Aa) but are dissimilar to those of the three other existing parasporins. Our results suggest that the soil of northern Vietnam is a good reservoir of parasporin-producing B. thuringiensis.Key words: Bacillus thuringiensis, soil isolate, Vietnam, mosquitocidal activity, parasporin, cancer-cell-killing activity.

Characterization of the anti-cancer-cell parasporal proteins of aBacillus thuringiensisisolate

2000 ◽  
Vol 46 (10) ◽  
pp. 913-919 ◽  
Author(s):  
Satoko Yamashita ◽  
Tetsuyuki Akao ◽  
Eiichi Mizuki ◽  
Hiroyuki Saitoh ◽  
Kazuhiko Higuchi ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Cancer Cell ◽  
Human Cancer ◽  
Proteolytic Processing ◽  
Proteinase K ◽  
Major Protein ◽  
Human Cancer Cell ◽  
Soil Isolate ◽  
Anti Cancer

An unusual activity, associated with non-insecticidal and non-haemolytic parasporal inclusion proteins of a Bacillus thuringiensis soil isolate, designated 89-T-26-17, was characterized. The parasporal inclusion of this isolate was bipyramidal, rounded at both ends, containing proteins of 180, 150, 120, 100, and 88 kDa. No homologies with the Cry and Cyt proteins of B. thuringiensis were detected based on N-terminal sequences. Proteolytic processing of the inclusion proteins by proteinase K, trypsin, and chymotrypsin produced a major protein of 64 kDa exhibiting cytocidal activity against human leukaemic T cells and uterus cervix cancer (HeLa) cells. The protease-activated proteins showed no cytotoxicity to normal T cells.Key words: Bacillus thuringiensis parasporal inclusion, non-insecticidal, non-haemolytic, cytocidal activity, human cancer cell.

Identification of Parasporin Genes in Vietnamese Isolates of Bacillus thuringiensis

2008 ◽  
Vol 63 (1-2) ◽  
pp. 139-143 ◽  
Author(s):  
Koichi Yasutake ◽  
Akiko Uemori ◽  
Ngo D. Binh ◽  
Eiichi Mizuki ◽  
Michio Ohba
Keyword(s):  
Bacillus Thuringiensis ◽  
Cancer Cells ◽  
Human Cancer ◽  
Nucleotide Sequence Analysis ◽  
Hep G2 ◽  
Normal Cells ◽  
Proteolytic Activation ◽  
Human Cancer Cells ◽  
Genes Encoding

Four genes encoding parasporins, cytotoxins preferentially killing human cancer cells in vitro, were isolated from four Vietnamese strains of Bacillus thuringiensis. Nucleotide sequence analysis revealed that: (1) three genes fall into the two known classes, ps1Aa and ps1Ab, and (2) another one belongs to ps1Ac, a novel gene class established in this study. Upon proteolytic activation, parasporal protein of the organism with ps1Ac exhibited strong cytocidal activity against human cancer cells, HeLa and Hep G2, but not to non-cancer normal cells, UtSMC and HC.

scholarly journals Secretoglobin 3A2 eliminates human cancer cells through pyroptosis

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Shigetoshi Yokoyama ◽  
Shun Nakayama ◽  
Lei Xu ◽  
Aprile L. Pilon ◽  
Shioko Kimura
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Innate Immune ◽  
Innate Immune Cells ◽  
Lung Cancer Cell ◽  
Small Cell Lung ◽  
Human Cancer Cells

AbstractNon-canonical inflammasome activation that recognizes intracellular lipopolysaccharide (LPS) causes pyroptosis, the inflammatory death of innate immune cells. The role of pyroptosis in innate immune cells is to rapidly eliminate pathogen-infected cells and limit the replication niche in the host body. Whether this rapid cell elimination process of pyroptosis plays a role in elimination of cancer cells is largely unknown. Our earlier study demonstrated that a multi-functional secreted protein, secretoglobin (SCGB) 3A2, chaperones LPS to cytosol, and activates caspase-11 and the non-canonical inflammasome pathway, leading to pyroptosis. Here we show that SCGB3A2 exhibits marked anti-cancer activity against 5 out of 11 of human non-small cell lung cancer cell lines in mouse xenographs, while no effect was observed in 6 of 6 small cell lung cancer cell lines examined. All SCGB3A2-LPS-sensitive cells express syndecan 1 (SDC1), a SCGB3A2 cell surface receptor, and caspase-4 (CASP4), a critical component of the non-canonical inflammasome pathway. Two epithelial-derived colon cancer cell lines expressing SDC1 and CASP4 were also susceptible to SCGB3A2-LPS treatment. TCGA analysis revealed that lung adenocarcinoma patients with higher SCGB3A2 mRNA levels exhibited better survival. These data suggest that SCGB3A2 uses the machinery of pyroptosis for the elimination of human cancer cells via the non-canonical inflammasome pathway, and that SCGB3A2 may serve as a novel therapeutic to treat cancer, perhaps in combination with immuno and/or targeted therapies.

scholarly journals Chimeric antigen receptors that trigger phagocytosis

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Meghan A Morrissey ◽  
Adam P Williamson ◽  
Adriana M Steinbach ◽  
Edward W Roberts ◽  
Nadja Kern ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Immune Cell ◽  
Human Cancer ◽  
Antibody Fragment ◽  
Cell Number ◽  
Antigen Receptors ◽  
Chimeric Antigen Receptors ◽  
Cell Therapies ◽  
Human Cancer Cells

Chimeric antigen receptors (CARs) are synthetic receptors that reprogram T cells to kill cancer. The success of CAR-T cell therapies highlights the promise of programmed immunity and suggests that applying CAR strategies to other immune cell lineages may be beneficial. Here, we engineered a family of Chimeric Antigen Receptors for Phagocytosis (CAR-Ps) that direct macrophages to engulf specific targets, including cancer cells. CAR-Ps consist of an extracellular antibody fragment, which can be modified to direct CAR-P activity towards specific antigens. By screening a panel of engulfment receptor intracellular domains, we found that the cytosolic domains from Megf10 and FcRɣ robustly triggered engulfment independently of their native extracellular domain. We show that CAR-Ps drive specific engulfment of antigen-coated synthetic particles and whole human cancer cells. Addition of a tandem PI3K recruitment domain increased cancer cell engulfment. Finally, we show that CAR-P expressing murine macrophages reduce cancer cell number in co-culture by over 40%.

The anti-cancer effect of amygdalin on human cancer cell lines

2019 ◽  
Vol 46 (2) ◽  
pp. 2059-2066 ◽  
Author(s):  
Asghar Arshi ◽  
Sayed Mostafa Hosseini ◽  
Fataneh Saleh Khaje Hosseini ◽  
Zahra Yousefnejad Amiri ◽  
Fatemeh Sadat Hosseini ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Anti Cancer

scholarly journals The role of membrane-bound metal ions in toxicity of a human cancer cell-active pore-forming toxin Cry41Aa from Bacillus thuringiensis

Toxicon ◽  
2019 ◽  
Vol 167 ◽  
pp. 123-133 ◽  
Author(s):  
Barbara Domanska ◽  
Eva Fortea ◽  
Michelle J. West ◽  
Jean-Louis Schwartz ◽  
Neil Crickmore
Keyword(s):  
Bacillus Thuringiensis ◽  
Metal Ions ◽  
Cancer Cell ◽  
Human Cancer ◽  
Human Cancer Cell ◽  
Membrane Bound

scholarly journals T-cell co-stimulation in combination with targeting FAK drives enhanced anti-tumor immunity

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Marta Canel ◽  
David Taggart ◽  
Andrew H Sims ◽  
David W Lonergan ◽  
Irene C Waizenegger ◽  
...  
Keyword(s):  
T Cell ◽  
Cancer Cell ◽  
Tumor Immunity ◽  
Checkpoint Inhibitors ◽  
Human Cancer ◽  
Complete Regression ◽  
Murine Tumors ◽  
Epithelial Cancers ◽  
Human Cancer Cells ◽  
Cell Expression

Focal Adhesion Kinase (FAK) inhibitors are currently undergoing clinical testing in combination with anti-PD-1 immune checkpoint inhibitors. However, which patients are most likely to benefit from FAK inhibitors, and what the optimal FAK/immunotherapy combinations are, is currently unknown. We identify that cancer cell expression of the T-cell co-stimulatory ligand CD80 sensitizes murine tumors to a FAK inhibitor and show that CD80 is expressed by human cancer cells originating from both solid epithelial cancers and some hematological malignancies in which FAK inhibitors have not been tested clinically. In the absence of CD80, we identify that targeting alternative T-cell co-stimulatory receptors, in particular OX-40 and 4-1BB in combination with FAK, can drive enhanced anti-tumor immunity and even complete regression of murine tumors. Our findings provide rationale supporting the clinical development of FAK inhibitors in combination with patient selection based on cancer cell CD80 expression, and alternatively with therapies targeting T-cell co-stimulatory pathways.

scholarly journals Momordica charantiaExtract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Chia-Jung Li ◽  
Shih-Fang Tsang ◽  
Chun-Hao Tsai ◽  
Hsin-Yi Tsai ◽  
Jong-Ho Chyuan ◽  
...  
Keyword(s):  
Cell Death ◽  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Dna Fragmentation ◽  
Human Cancer ◽  
Momordica Charantia ◽  
Carcinoma Cells ◽  
Human Cancer Cells ◽  
Adenocarcinoma Cells ◽  
Anti Cancer

Plants are an invaluable source of potential new anti-cancer drugs.Momordica charantiais one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness ofMomordica charantia, methanol extract ofMomordica charantia(MCME) was used to evaluate the cytotoxic activity on four human cancer cell lines, Hone-1 nasopharyngeal carcinoma cells, AGS gastric adenocarcinoma cells, HCT-116 colorectal carcinoma cells, and CL1-0 lung adenocarcinoma cells, in this study. MCME showed cytotoxic activity towards all cancer cells tested, with the approximate IC50ranging from 0.25 to 0.35 mg/mL at 24 h. MCME induced cell death was found to be time-dependent in these cells. Apoptosis was demonstrated by DAPI staining and DNA fragmentation analysis using agarose gel electrophoresis. MCME activated caspase-3 and enhanced the cleavage of downstream DFF45 and PARP, subsequently leading to DNA fragmentation and nuclear condensation. The apoptogenic protein, Bax, was increased, whereas Bcl-2 was decreased after treating for 24 h in all cancer cells, indicating the involvement of mitochondrial pathway in MCME-induced cell death. These findings indicate that MCME has cytotoxic effects on human cancer cells and exhibits promising anti-cancer activity by triggering apoptosis through the regulation of caspases and mitochondria.

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