Lotus corniculatuscondensed tannins decrease in vivo populations of proteolytic bacteria and affect nitrogen metabolism in the rumen of sheep

2002 ◽  
Vol 48 (10) ◽  
pp. 911-921 ◽  
Author(s):  
B R Min ◽  
G T Attwood ◽  
K Reilly ◽  
W Sun ◽  
J S Peters ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Lotus Corniculatus ◽  
Ammonia Nitrogen ◽  
Pool Size ◽  
Rumen Bacteria ◽  
Forage Legumes ◽  
Bacterial Populations ◽  
Nitrogen Pool ◽  
Proteolytic Bacteria

Condensed tannins in forage legumes improve the nutrition of sheep by reducing ruminal degradation of plant protein and increasing crude protein flow to the intestine. However, the effects of condensed tannins in forage legumes on rumen bacterial populations in vivo are poorly understood. The aim of this study was to investigate the specific effects of condensed tannins from Lotus corniculatus on four proteolytic rumen bacteria in sheep during and after transition from a ryegrass (Lolium perenne) – white clover (Trifolium repens) diet (i.e., low condensed tannins) to a Lotus corniculatus diet (i.e., higher condensed tannins). The bacterial populations were quantified using a competitive polymerase chain reaction. Lotus corniculatus was fed with or without ruminal infusions of polyethylene glycol (PEG), which binds to and inactivates condensed tannins, enabling the effect of condensed tannins on bacterial populations to be examined. When sheep fed on ryegrass – white clover, populations of Clostridium proteoclasticum B316T, Butyrivibrio fibrisolvens C211a, Eubacterium sp. C12b, and Streptococcus bovis B315 were 1.5 × 108, 1.1 × 106, 4.6 × 108, and 7.1 × 106mL–1, respectively. When the diet was changed to Lotus corniculatus, the average populations (after 8–120 h) of C. proteoclasticum, B. fibrisolvens, Eubacterium sp., and S. bovis decreased (P < 0.001) to 2.4 × 107, 1.1 × 105, 1.1 × 108, and 2.5 × 105mL–1, respectively. When PEG was infused into the rumen of sheep fed Lotus corniculatus, the populations of C. proteoclasticum, B. fibrisolvens, Eubacterium sp., and S. bovis were higher (P < 0.01–0.001) than in sheep fed Lotus corniculatus without the PEG infusion, with average populations (after 8–120 h) of 4.9 × 107, 3.8 × 105, 1.9 ×108, and 1.0 ×106, respectively. Sheep fed the Lotus corniculatus diet had lower rumen proteinase activity, ammonia, and soluble nitrogen (P < 0.05–0.001) than sheep that were fed Lotus corniculatus plus PEG. The Lotus corniculatus diet reduced rumen nitrogen digestibility (P < 0.05) and ammonia pool size and increased the flow of undegraded feed nitrogen to the abomasum. The nitrogen intake, rumen non-ammonia nitrogen pool size, rumen microbial non-ammonia nitrogen pool size, and abomasal microbial non-ammonia nitrogen fluxes were similar both in sheep fed only Lotus corniculatus and in sheep fed Lotus corniculatus plus PEG, but nonmicrobial non-ammonia nitrogen flux to the abomasum was higher (P < 0.01) for the sheep fed only Lotus corniculatus. Although condensed tannins in Lotus corniculatus reduced the populations of some proteolytic bacteria, total ruminal microbial protein and microbial protein outflow to the abomasum were unchanged, suggesting a species-specific effect of condensed tannins on bacteria in the rumen. Key words: condensed, tannin, rumen, bacteria, PCR.

Effect of condensed tannins in Lotus corniculatus on the nutritive value of pasture for sheep

1997 ◽  
Vol 128 (3) ◽  
pp. 365-372 ◽  
Author(s):  
G. C. WAGHORN ◽  
I. D. SHELTON
Keyword(s):  
Soluble Protein ◽  
Condensed Tannins ◽  
Dry Matter ◽  
Nutritive Value ◽  
Experimental Period ◽  
Lotus Corniculatus ◽  
Feeding Trial ◽  
Wool Growth

The extent to which condensed tannins (CT) in Lotus corniculatus were able to affect protein degradation and the nutritive value of pasture were evaluated at Palmerston North, New Zealand in 1992 in an indoor feeding trial with sheep and using in vitro incubations. The feeding trial involved three groups of seven young wether sheep held indoors in metabolism crates for the 32-day experimental period with one group fed freshly cut ryegrass/clover pasture (Pasture group) whilst the other two groups received a mixture of c. 37% freshly cut Lotus corniculatus and 63% pasture (dry matter (DM) basis). One of the latter groups was given twice daily oral drenches of polyethylene glycol (PEG) to bind with and remove the effects of the CT (PEG group) whilst the remaining sheep (Tannin group) were not drenched. Intakes of sheep given the mixed diets were constrained to that of the pasture group. The CT concentration in the lotus was 26·1 g/kg DM so that the mixed diets contained c. 10g CT/kg DM. Effects of CT on digestion and sheep performance were minimal. Apparent digestibility of N was lower in the Tannin group (76·4%) than PEG (79·8%) or Pasture (79·4%) sheep (P<0·001), but there were no differences in DM digestibility (75%) or wool growth from mid-side patches. Rumen ammonia and soluble protein concentrations appeared to be similar in PEG and Tannin sheep and volatile fatty acid (VFA) concentrations were similar in all groups (100 mmol/l).For incubations in vitro, several ratios of Lotus corniculatus: pasture were used to determine the effects of CT on precipitation of soluble proteins and the net yield of ammonia and gas with and without PEG. Homogenates of Lotus corniculatus leaves and pasture showed that 50% of soluble protein was precipitated by 1·3% CT in homogenate DM and there was a substantial decrease in in vitro degradation to ammonia when lotus leaf accounted for 33% or more of DM with pasture. This work emphasises the need for caution when extrapolating from in vitro to the in vivo situation and suggests that 1% CT from Lotus corniculatus may be insufficient to affect the nutritive value of fresh forages for sheep.

scholarly journals Effects of Condensed Tannins Supplementation on Animal Performance, Phylogenetic Microbial Changes, and In Vitro Methane Emissions in Steers Grazing Winter Wheat

Animals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2391
Author(s):  
Byeng R. Min ◽  
William E. Pinchak ◽  
Michael E. Hume ◽  
Robin C. Anderson
Keyword(s):  
Winter Wheat ◽  
Condensed Tannins ◽  
Average Daily Gain ◽  
Control Groups ◽  
Banding Patterns ◽  
Bacterial Populations ◽  
Gas Potential ◽  
Daily Gain

Eighteen growing rumen-cannulated steers, with initial body weight (BW) of 167.4 ± 7.10 kg, were randomly allocated to one of three treatments that included a control (0% CT) and two CT treatment levels (0.05% and 0.07% condensed tannins (CT)/kg BW) with two replicates each. Both in vivo and in vitro experiments were conducted. In Exp. 1, final BW and average daily gain were greater (p < 0.05) for the 0.07% CT treatment compared to either 0.05% CT or control groups. Rumen bacterial populations in steers fed winter wheat in the absence of CT represented large proportions of the moderate-guanines and cytosines (GC) containing bacterial clusters with similarity coefficient (SC) ranging from 64% to 92% In the presence of CT on day 0, day 20, and day 60, however, the SC was 60% or greater (90% SC) with multiple bacterial band clusters as shown by the denaturing gel gradient electrophoresis banding patterns. In Exp. 2, in vitro total gas, potential gas, and CH4 productions decreased (p < 0.01) as CT supplementation increased in steers grazing wheat forage. These results suggested that the administration of CT improved BW gain and induced bacterial community changes in the rumen of steers grazing wheat forage.

scholarly journals In vivo 13C NMR studies of glucose catabolism by isolated rumen bacteria

1989 ◽  
Vol 29 (1) ◽  
pp. 83-88 ◽  
Author(s):  
J. P. Grivet ◽  
J. Stevani ◽  
G. Hannequart ◽  
M. Durand
Keyword(s):  
13C Nmr ◽  
Rumen Bacteria ◽  
Nmr Studies ◽  
Glucose Catabolism

scholarly journals Streptococcal Adhesion and Colonization

1997 ◽  
Vol 8 (2) ◽  
pp. 175-200 ◽  
Author(s):  
H.F. Jenkinson ◽  
RJ Lamont
Keyword(s):  
Immune Modulation ◽  
Rational Design ◽  
Repeated Sequence ◽  
Host Cells ◽  
Mammalian Host ◽  
Related Factors ◽  
Bacterial Populations ◽  
Acellular Vaccines

Streptococci express arrays of adhesins on their cell surfaces that facilitate adherence to substrates present in their natural environment within the mammalian host. A consequence of such promiscuous binding ability is that streptococcal cells may adhere simultaneously to a spectrum of substrates, including salivary glycoproteins, extracellular matrix and serum components, host cells, and other microbial cells. The multiplicity of streptococcal adherence interactions accounts, at least in part, for their success in colonizing the oral and epithelial surfaces of humans. Adhesion facilitates colonization and may be a precursor to tissue invasion and immune modulation, events that presage the development of disease. Many of the streptococcal adhesins and virulence-related factors are cell-wall-associated proteins containing repeated sequence blocks of amino acids. Linear sequences, both within the blocks and within non-repetitive regions of the proteins, have been implicated in substrate binding. Sequences and functions of these proteins among the streptococci have become assorted through gene duplication and horizontal transfer between bacterial populations. Several adhesins identified and characterized through in vitro binding assays have been analyzed for in vivo expression and function by means of animal models used for colonization and virulence. Information on the molecular structure of adhesins as related to their in vivo function will allow for the rational design of novel acellular vaccines, recombinant antibodies, and adhesion agonists for the future control or prevention of streptococcal colonization and streptococcal diseases.

scholarly journals The availability of inorganic sulphate in blood for sulphate conjugation of drugs in rat liver in vivo. (35S)Sulphate incorporation into harmol sulphate

1978 ◽  
Vol 172 (2) ◽  
pp. 247-251 ◽  
Author(s):  
G J Mulder ◽  
E Scholtens
Keyword(s):  
Plasma Concentration ◽  
Rat Liver ◽  
Biliary Excretion ◽  
Time Course ◽  
Specific Radioactivity ◽  
Pool Size ◽  
Inorganic Sulphate ◽  
Initial Decrease ◽  
Sulphate Conjugate

1. When Na235SO4 is injected intravenously in rats, it is immediately available for sulphate conjugation of the phenolic drug harmol (7-hydroxyl-1-methyl-9H-pyrido[3,4-b]indole) in the liver. This was established by following the time course of the biliary excretion of the sulphate conjugate of harmol, and the incorporation of [35S]sulphate into harmol sulphate. 2. During the 10min immediately after injection of Na235SO4 re-distribution of [35S]sulphate took place, which resulted in a rapid initial decrease in the plasma concentration of [35S]sulphate; a concomitant decrease in the amount of [35S]sulphate incorporated into harmol sulphate was observed, indicating that the co-substrate of sulphation, adenosine 3′-phosphate 5′-sulphatophosphate, equilibrates rapidly with [35S]sulphate in plasma. 3. The results suggest that the pool size of adenosine 3′-phosphate 5′-sulphatophosphate is very small; therefore the specific radioactivity of [35S]sulphate in plasma determines the specific radioactivity incorporated into sulphate esters at any time.

scholarly journals Antimicrobial Activity of Non-steroidal Anti-inflammatory Drugs on Biofilm: Current Evidence and Potential for Drug Repurposing

2021 ◽  
Vol 12 ◽  
Author(s):  
Rodrigo Cuiabano Paes Leme ◽  
Raquel Bandeira da Silva
Keyword(s):  
Bacterial Species ◽  
Drug Repurposing ◽  
Current Evidence ◽  
Pharmacokinetic Studies ◽  
Bacterial Populations ◽  
Human Pharmacokinetic ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

It has been demonstrated that some non-steroidal anti-inflammatory drugs (NSAIDs), like acetylsalicylic acid, diclofenac, and ibuprofen, have anti-biofilm activity in concentrations found in human pharmacokinetic studies, which could fuel an interest in repurposing these well tolerated drugs as adjunctive therapies for biofilm-related infections. Here we sought to review the currently available data on the anti-biofilm activity of NSAIDs and its relevance in a clinical context. We performed a systematic literature review to identify the most commonly tested NSAIDs drugs in the last 5 years, the bacterial species that have demonstrated to be responsive to their actions, and the emergence of resistance to these molecules. We found that most studies investigating NSAIDs’ activity against biofilms were in vitro, and frequently tested non-clinical bacterial isolates, which may not adequately represent the bacterial populations that cause clinically-relevant biofilm-related infections. Furthermore, studies concerning NSAIDs and antibiotic resistance are scarce, with divergent outcomes. Although the potential to use NSAIDs to control biofilm-related infections seems to be an exciting avenue, there is a paucity of studies that tested these drugs using appropriate in vivo models of biofilm infections or in controlled human clinical trials to support their repurposing as anti-biofilm agents.

scholarly journals Use of Tannin-Containing Plants as Antimicrobials Influencing the Animal Health

2022 ◽  
Vol 45 (2) ◽  
pp. 33-40
Author(s):  
Mohammed M Dakheel ◽  
Afnan A Al-Mnaser ◽  
Jessica Quijada ◽  
Martin J Woodward ◽  
Caroline Rymer
Keyword(s):  
Nutritional Quality ◽  
Condensed Tannins ◽  
Animal Health ◽  
Degree Of Polymerization ◽  
Gram Negative Bacteria ◽  
Animal Feeding ◽  
The Subject

The antimicrobial effects of diverse tannin-containing plants, particularly condensed tannins (CTs) produced from various plants, are the subject of this study. CT components can be determined using CT-specific procedures such the HCl-Butanol Acetone assay, Thiolysis reaction, and HPLC/MS analysis. These methods indicate CT contents, including mean degree of polymerization, the procyanidins and prodelphinidins ratio (PC/PD%), the isomers of trans- and cis-, and CT concentration. Tannin-containing plants possess antibacterial action, which can be attributed to their protein linkage technique, and tannin-type variations, particularly CTs extract and their PC/PD%. The effects of CT components on the development of Gram-positive and Gram-negative bacteria have been documented for their relative PC/PD%; this is regarded to be a key predictor of tannin characteristics in terms of antimicrobials. In conclusion, tannins, more specific CT compositions, have significant impacts on in vivo trials of animal productions and utilization of metabolites and fermentation in vitro experiments. These findings need further investigations to fully understand how CT-types act on animal feeding in terms of enhanced nutritional quality of animal diets, which may have implications for human and animal health.

scholarly journals The three dimensional structure of Bovine Salivary Protein 30b (BSP30b) and its interaction with specific rumen bacteria

2018 ◽  
Author(s):  
Heng Zhang ◽  
Judith Burrows ◽  
Graeme L. Card ◽  
Graeme Attwood ◽  
Thomas T. Wheeler ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Binding Proteins ◽  
Bacterial Species ◽  
Fluorescent Microscopy ◽  
Salivary Protein ◽  
Lipid Binding ◽  
Dimensional Structure ◽  
Rumen Bacteria ◽  
Abundant Protein

AbstractBovine Salivary Protein 30b (BSP30b) is a member of the tubular lipid-binding (TULIP) superfamily that includes the human bactericidal/permeability-increasing proteins (BPI), lipopolysaccharide binding proteins (LBP) and palate, lung, and nasal epithelium carcinoma-21 associated proteins (PLUNC). BSP30b is most closely related to the PLUNC family and is predominantly found in bovine saliva. There are four BSP30 isoforms (BSP30a-d) and collectively, they are the most abundant protein component of bovine saliva. The PLUNC family members are proposed to be lipid binding proteins, although in most cases their lipid ligands are unknown. Here, we present the X-ray crystal structure of BSP30b at 2.0 Å resolution. We used a double methionine mutant and Se-Met SAD phasing to solve the structure. The structure adopts a curved cylindrical form with a hydrophobic channel formed by an α/β wrap, which is consistent with the TULIP superfamily. The structure of BSP30b in complex with oleic acid is also presented where the ligand is accommodated within the hydrophobic channel. The electron density for oleic acid suggests that the ligand is only partially occupied in the binding site implying that oleic acid may not be the preferred ligand. GFP-tagged BSP30b binds to the surface of olive oil droplets, as observed under fluorescent microscopy, and acts as a surfactant consistent with its association with decreased susceptibility to bloat in cattle. Bacteria extracted directly from bovine rumen contents indicate that the GFP_BSP30b fusion protein binds to a small number of selected bacterial species in vivo. These results suggest that BSP30b may bind to bacterial lipids from specific species and that this abundant protein may have important biological roles via interacting with rumen bacteria during feeding and rumination.

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