Simplified media for spiroplasmas associated with tabanid flies

2002 ◽  
Vol 48 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Rebekah W Moulder ◽  
Frank E French ◽  
C J Chang
Keyword(s):  
Costa Rica ◽  
Growth Medium ◽  
The Other ◽  
Group Viii ◽  
Field Isolates ◽  
Sustained Growth ◽  
Significant Difference ◽  
Horse Flies ◽  
Horse Fly ◽  
Made In

Traditionally, isolation, maintenance, and testing of Spiroplasma species (Mollicutes: Entomoplasmatales) from horse flies (Tabanus spp.) and deer flies (Chrysops spp.) (Diptera: Tabanidae) have been accomplished in the complex M1D medium. A relatively inexpensive, simplified medium for tabanid spiroplasmas could expedite procedures that require large quantities of growth medium. Nine strains of spiroplasmas, eight from tabanids and one from mosquitoes, were cultured in three simplified broth media, R2, R8-1, and C-3G, and in M1D. There was no significant difference in the rate of spiroplasma growth in M1D and the three simplified media. R2medium supported the growth of tabanid spiroplasmas more consistently and with better morphology through 10 subcultures than did the other simplified media. Primary isolations were made in R2medium from tabanids collected (i) in Georgia, U.S.A., with 10 isolations from 10 flies and (ii) in coastal Costa Rica, with isolation rates of 70% (28/40) and 73% (27/37), respectively, for R2and M1D. Of the seven group VIII field isolates from Costa Rica, four were capable of sustained growth in R2, and three were triply cloned in this simplified medium. These results suggest that the simplified medium R2is suitable for many procedures with tabanid spiroplasmas.Key words: simplified media, Mollicutes, spiroplasma, tabanid, horse fly.

scholarly journals Dimensional stability of distances between posterior teeth in maxillary complete dentures

2006 ◽  
Vol 20 (3) ◽  
pp. 241-246 ◽  
Author(s):  
Rafael Leonardo Xediek Consani ◽  
Marcelo Ferraz Mesquita ◽  
Lourenço Correr-Sobrinho ◽  
Maurício Tanji
Keyword(s):  
Dimensional Stability ◽  
Storage Period ◽  
Acrylic Resin ◽  
The Other ◽  
Complete Dentures ◽  
Posterior Teeth ◽  
Anterior Teeth ◽  
Significant Difference ◽  
Cooling Methods ◽  
Made In

The aim of this study was to assess the displacement of posterior teeth in maxillary complete dentures stored in water at 37°C. Twenty acrylic resin-based maxillary complete dentures were constructed with the anterior teeth arranged in normal overlap and the posterior teeth in Angle class I. Metallic pins were placed on the labial cusp of the first premolars (PM), and on the mesiolabial cusp of the second molars (M). The final acrylic resin pressing was made in a metallic flask with aid of the RS tension system, and polymerized in a moist-hot cycle at 74°C for 9 hours. The dentures were deflasked after cooling in their own polymerizing water or after cooling in polymerizing water plus bench storage for 3 hours, and stored in water at 37°C for periods of 7, 30, and 90 days. Following deflasking and after each storage period tested, the PM-PM (premolar to premolar), M-M (molar to molar), LPM-LM (left premolar to left molar), and RPM-RM (right premolar to right molar) distances were measured with an STM Olympus microscope, with an accuracy of 0.0005 mm. Collected data were submitted to ANOVA and Tukey's test (5%). There was no statistically significant difference for the PM-PM, M-M, and LPM-LM distances after all storage periods when the flask cooling methods were considered. With exception of the RPM-RM distance after the 30-days water plus bench storage period, the other distances remained statistically stable.

scholarly journals Physical activity of school children and their parents in Vojvodina

2005 ◽  
Vol 58 (1-2) ◽  
pp. 52-56 ◽  
Author(s):  
Olja Niciforovic-Surkovic ◽  
Erzebet Ac-Nikolic ◽  
Snezana Ukropina ◽  
Vesna Mijatovic-Jovanovic
Keyword(s):  
Physical Activity ◽  
Health Promotion ◽  
Elementary Schools ◽  
Growth And Development ◽  
The Other ◽  
Young Population ◽  
Education And Health ◽  
Significant Difference ◽  
Negative Effect ◽  
Made In

Introduction. Physical activity is very important for normal physical and psychic growth and development. Recent studies in developed and developing countries have shown that today's schoolchildren are spending more and more time in front of the TV and computer, and less and less are engaged in sports. Such lifestyle has a negative effect on health. That is why it is very important for children to develop active habits for life, in order to sustain and promote their health. Material and methods. The study included, a random sample of 210 children from 4 elementary schools and their parents in Vojvodina. Specially designed questionnaires were used. Results and Discussion. The obtained results showed that 75% of schoolchildren are engaged in physical activity, but only 56.5% play sports regularly (every day or 2-3 times a week). There is a significant difference between boys and girls: about 75% of boys and 35% of girls are engaged in physical activity regularly. Similar results have been obtained by other authors (O'Reilly and Shelley, Faucette, Heath etc). Common reasons for physical activity are health, fitness and fun. Pupil's attitude to sports significantly depends on their parent's opinion about physical activity and also on their attitude to recreation in their future life. Parents are not active as their children, only 40% of fathers and 30% of mothers are engaged in various activities regularly. Conclusion. This study showed that schoolchildren are engaged in physical activity, but not enough. On the other hand, their parents are very inactive, although they see the importance of it. Considering the fact that present recreational habits are correlated with the attitude to recreation in the future, an additional effort should be made in health education and health promotion in young population. .

scholarly journals In vitro study of effect of solvent on root canal retreatment

2002 ◽  
Vol 13 (3) ◽  
pp. 208-211 ◽  
Author(s):  
Kazumi Onaga Nagayama Oyama ◽  
Evandro Luiz Siqueira ◽  
Marcelo dos Santos
Keyword(s):  
In Vitro Study ◽  
The Other ◽  
Orange Oil ◽  
Root Canals ◽  
Effect Of Solvent ◽  
Gutta Percha ◽  
Root Canal Retreatment ◽  
Significant Difference ◽  
Made In

The aim of this study was to assess the effectiveness of five different solvents: xylol, eucalyptol, halothane, chloroform and orange oil on softening gutta-percha in simulated root canals. One drop of solvent was placed into a reservoir made in a simulated canal whose channel was previously instrumented and filled with gutta-percha and N-Rickert sealer. After 5 min, softening was evaluated for each solvent by the penetration of a spreader while applying force with a 442 Instron apparatus to reach a depth of 5 mm. The results were analyzed statistically by the Kruskal-Wallis test. Xylol and orange oil were better in softening gutta-percha than the other solvents. There was no significant difference between xylol and orange oil, but these were statistically different from eucalyptol, halothane and chloroform (p<0.01).

scholarly journals A study of transferable skills for Work based Learning (WBL) Assessment

2015 ◽  
Vol 6 (4) ◽  
pp. 59-65
Author(s):  
Abdool Qaiyum Mohabuth
Keyword(s):  
University Students ◽  
The Other ◽  
Good Representation ◽  
Male And Female ◽  
Transferable Skills ◽  
Quantitative Technique ◽  
Formal Assessment ◽  
Work Settings ◽  
Significant Difference ◽  
Made In

Transferable skills are learnt abilities which are mainly acquired when experiencing work. University students have the opportunities to develop the knowledge and aptitude at work when they undertake WBL placement during their studies. There is a range of transferable skills which students may acquire at their placement settings. Assessing the achievement of students on practice learning based on the transferable skills is regarded as being complex and tedious due to the variability of placement settings. No attempt has been made in investigating whether these skills are assessable at practice settings. This study seeks to define a set of generic transferable skills that can be assessed during WBL practice. Quantitative technique was used involving the design of two questionnaires. One was administered to University of Mauritius students who have undertaken WBL practice and the other was slightly modified, destined to mentors who have supervised and assessed students at placement settings. To obtain a good representation of the student’s population, the sample considered was stratified over four Faculties. As for the mentors, probability sampling was considered. Findings revealed that transferable skills may be subject to formal assessment at practice settings. Hypothesis tested indicate that there was no significant difference among male and female as regards to the application of transferable skills for formal assessment. A list of core transferable skills that are assessable at any practice settings has been defined after taking into account their degree of being generic, extent of acquisition at work settings and their consideration for formal assessment. Both students and mentors assert that these transferable skills are accessible at work settings and require commitment and energy to be acquired successfully.

scholarly journals Livros de ocorrência: Violência e indisciplina em escolas de território vulnerável

2016 ◽  
Vol 24 ◽  
pp. 126
Author(s):  
Claudia Lemos Vóvio ◽  
Vanda Mendes Ribeiro ◽  
Luiz Carlos Novaes ◽  
Maria Helena Bravo
Keyword(s):  
School Environment ◽  
Exposure To Violence ◽  
External Factors ◽  
The Other ◽  
Moral Integrity ◽  
School Professionals ◽  
Significant Difference ◽  
The One ◽  
Disciplinary System ◽  
Made In

This article suggests categories for the analysis of registries made in books of occurrences within state managed schools. Furthermore it verifies the categories’ pertinence for the comprehension of schooling environment in these institutions. In a context of vulnerable territories in which these institutions are set, violence and indiscipline are considered relevant to grasp what goes on in their midst. The document basis counts seven such books in each school, produced between 2007 and 2013. A significant difference between schools springs up, both in terms of quantity of registries and in terms of categories distribution. Bearing in mind these differences and the weight of external factors on each school environment, both frequently notify events that threaten the physical and moral integrity of students, which, in the perception of those making the reports, denote unsafe environments with exposure to violence and incidence of problems relating to the mediation of conflicts and democratic bonding. In one of the schools there is a greater emphasis on indiscipline notifications which may express, on the one hand, the attention of school professionals to events that put at risk the maintenance of authority and the fulfilment of conviviality norms in the sphere of relations between students and teachers and, on the other, the relation between students via-a-vis the established disciplinary system. In the other school there are a noteworthy number of notifications relating to the schooling routine relevant to the overall organization of the institution. The results of the research suggest the necessity for administrative departments’ support so that schools in vulnerable territories are able to efficiently face situations that affect their schooling environments. 

Writing in Aphasia Rehabilitation: Cursive vs Manuscript

1976 ◽  
Vol 41 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Daniel R. Boone ◽  
Harold M. Friedman
Keyword(s):  
Correct Response ◽  
The Other ◽  
Read Aloud ◽  
Writing Style ◽  
Reading And Writing ◽  
Manual Responses ◽  
Significant Difference ◽  
Written Form ◽  
The Individual ◽  
Read Number

Reading and writing performance was observed in 30 adult aphasic patients to determine whether there was a significant difference when stimuli and manual responses were varied in the written form: cursive versus manuscript. Patients were asked to read aloud 10 words written cursively and 10 words written in manuscript form. They were then asked to write on dictation 10 word responses using cursive writing and 10 words using manuscript writing. Number of words correctly read, number of words correctly written, and number of letters correctly written in the proper sequence were tallied for both cursive and manuscript writing tasks for each patient. Results indicated no significant difference in correct response between cursive and manuscript writing style for these aphasic patients as a group; however, it was noted that individual patients varied widely in their success using one writing form over the other. It appeared that since neither writing form showed better facilitation of performance, the writing style used should be determined according to the individual patient’s own preference and best performance.

Multi-Center Study of Thromboplastin Calibration Precision – Influence of Reagent Species, Composition, and International Sensitivity Index (ISI)

1993 ◽  
Vol 69 (01) ◽  
pp. 035-040 ◽  
Author(s):  
A M H P van den Besselaar ◽  
R M Bertina
Keyword(s):  
Oral Anticoagulants ◽  
International Normalized Ratio ◽  
The Other ◽  
World Health ◽  
Rabbit Brain ◽  
Sensitivity Index ◽  
Bovine Plasma ◽  
Significant Difference ◽  
The Mean ◽  
International Sensitivity Index

SummaryFour thromboplastin reagents were tested by 18 laboratories in Europe, North-America, and Australasia, according to a detailed protocol. One thromboplastin was the International Reference Preparation for ox brain thromboplastin combined with adsorbed bovine plasma (coded OBT/79), and the second was a certified reference material for rabbit brain thromboplastin, plain (coded CRM 149R). The other two thromboplastin reagents were another rabbit plain brain thromboplastin (RP) with a lower ISI than CRM 149R and a rabbit brain thromboplastin combined with adsorbed bovine plasma (RC). Calibration of the latter two reagents was performed according to methods recommended by the World Health Organization (W. H. O.).The purpose of this study was to answer the following questions: 1) Is the calibration of the RC reagent more precise against the bovine/combined (OBT/79) than against the rabbit/plain reagent (CRM 149R)? 2) Is the precision of calibration influenced by the magnitude of the International Sensitivity Index (ISI)?The lowest inter-laboratory variation of ISI was observed in the calibration of the rabbit/plain reagent (RP) against the other rabbit/plain reagent (CRM 149R) (CV 1.6%). The highest interlaboratory variation was obtained in the calibration of rabbit/plain (RP) against bovine/combined (OBT/79) (CV 5.1%). In the calibration of the rabbit/combined (RC) reagent, there was no difference in precision between OBT/79 (CV 4.3%) and CRM 149R (CV 4.2%). Furthermore, there was no significant difference in the precision of the ISI of RC obtained with CRM 149R (ISI = 1.343) and the rabbit/plain (RP) reagent with ISI = 1.14. In conclusion, the calibration of RC could be performed with similar precision with either OBT/79 or CRM 149R, or RP.The mean ISI values calculated with OBT/79 and CRM 149R were practically identical, indicating that there is no bias in the ISI of these reference preparations and that these reference preparations have been stable since their original calibration studies in 1979 and 1987, respectively.International Normalized Ratio (INR) equivalents were calculated for a lyophilized control plasma derived from patients treated with oral anticoagulants. There were small but significant differences in the mean INR equivalents between the bovine and rabbit thromboplastins. There were no differences in the interlaboratory variation of the INR equivalents, when the four thromboplastins were compared.

IMMUNOLOGICAL REACTIVITY OF INSULIN IN HUMAN SERUM

1966 ◽  
Vol 53 (4) ◽  
pp. 673-680 ◽  
Author(s):  
Torsten Deckert ◽  
Kai R. Jorgensen
Keyword(s):  
Normal Subjects ◽  
The Other ◽  
Human Pancreas ◽  
Porcine Pancreas ◽  
Crystalline Insulin ◽  
Endogenous Insulin ◽  
Significant Difference ◽  
Human Sera ◽  
Normal Human ◽  
The One

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.

On the Future of the Financial Redistribution across Member States in the EU

2006 ◽  
Vol 56 (1) ◽  
pp. 1-43
Author(s):  
Sándor Richter
Keyword(s):  
Value Added ◽  
The Other ◽  
Current Debate ◽  
Member States ◽  
New Member States ◽  
Financial Position ◽  
Cross Member ◽  
Expenditure Structure ◽  
The Eu ◽  
Made In

The order and modalities of cross-member state redistribution as well as the net financial position of the member states are one of the most widely discussed aspects of European integration. The paper addresses selected issues in the current debate on the EU budget for the period 2007 to 2013 and introduces four scenarios. The first is identical to the European Commission's proposal; the second is based on reducing the budget to 1% of the EU's GNI, as proposed by the six net-payer countries, while maintaining the expenditure structure of the Commission's proposal. The next two scenarios represent radical reforms: one of them also features a '1% EU GNI'; however, the expenditures for providing 'EU-wide value-added' are left unchanged and it is envisaged that the requisite cuts will be made in the expenditures earmarked for cohesion. The other reform scenario is different from the former one in that the cohesion-related expenditures are left unchanged and the expenditures for providing 'EU-wide value-added' are reduced. After the comparison of the various scenarios, the allocation of transfers to the new member states in terms of the conditions prevailing in the different scenarios is analysed.

Physical and Mechanical Properties of Tenun Pahang Fabrics using Alternative Yarns

2016 ◽  
Vol 13 (2) ◽  
pp. 67
Author(s):  
Engku Liyana Zafirah Engku Mohd Suhaimi ◽  
Jamil Salleh ◽  
Suzaini Abd Ghani ◽  
Mohamad Faizul Yahya ◽  
Mohd Rozi Ahmad
Keyword(s):  
Mechanical Properties ◽  
Physical And Mechanical Properties ◽  
The Other ◽  
Silk Fabrics ◽  
Recovery Angle ◽  
Made In

An investigation on the properties of Tenun Pahang fabric performances using alternative yarns was conducted. The studies were made in order to evaluate whether the Tenun Pahang fabric could be produced economically and at the same time maintain the fabric quality. Traditional Tenun Pahang fabric uses silk for both warp and weft. For this project, two alternative yarns were used which were bamboo and modal, which were a little lower in cost compared to silk. These yarns were woven with two variations, one with the yarns as weft only while maintaining the silk warp and the other with both warp and weft using the alternative yarns. Four (4) physical testings and three (3) mechanical testings conducted on the fabric samples. The fabric samples were evaluated including weight, thickness, thread density, crease recovery angle, stiffness and drapability. The results show that modal/silk and bamboo silk fabrics are comparable in terms of stiffness and drapability, hence they have the potential to replace 100% silk Tenun Pahang.

Sign in / Sign up

Export Citation Format

Share Document