Mycelial differentiation and spore formation by Streptomyces brasiliensis in submerged culture

2001 ◽  
Vol 47 (11) ◽  
pp. 1042-1047 ◽  
Author(s):  
Begoña Rueda ◽  
Elisa M Miguélez ◽  
Carlos Hardisson ◽  
Manuel B Manzanal
Keyword(s):  
Electron Microscopy ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Ultrastructural Changes ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Solid Media ◽  
Aerial Hyphae

Streptomyces brasiliensis ATCC 23727 showed extensive sporulation when cultured in a liquid medium containing galactose and glutamic acid as carbon and nitrogen sources. Sporogenic hyphae formed under these conditions were morphologically similar and developmentally equivalent to aerial hyphae and metamorphosed into chains of spores by following a sequence of ultrastructural changes similar to that observed during growth on solid media. In addition, our electron microscopy study revealed two previously unrecognized aspects of hyphal development in streptomycetes: the formation of sporogenic hyphae was always preceded by changes in the structure of the nucleoid, and the sheath that characteristically covered these hyphae was not deposited coincidently with wall formation in the apical growing portion of the hypha.Key words: Streptomyces brasiliensis, mycelial differentiation, sporulation.

scholarly journals Development of a Semiselective Medium for Detection of Nalanthamala psidii, Causal Agent of Wilt of Guava

Plant Disease ◽  
2013 ◽  
Vol 97 (9) ◽  
pp. 1132-1136 ◽  
Author(s):  
C. F. Hong ◽  
H. Y. Hsieh ◽  
C. T. Chen ◽  
H. C. Huang
Keyword(s):  
Mycelial Growth ◽  
Nitrogen Sources ◽  
Psidium Guajava ◽  
Epidemiological Studies ◽  
Potato Dextrose Agar ◽  
Infested Soil ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Aerial Hyphae ◽  
Important Disease

Guava wilt, caused by Nalanthamala psidii, has become an important disease of guava (Psidium guajava) in Taiwan since the 1970s. This study was conducted to develop a semiselective medium for detecting N. psidii in soil and in tissues of diseased guava trees. Among 9 carbon and 21 nitrogen compounds tested in a modified Czapek-Dox medium, the most effective carbon and nitrogen sources for mycelial growth of N. psidii were sucrose and glycine, respectively. Among eight fungicides tested, iprodione at 5 μg ml–1 and azoxystrobin at 1 μg ml–1 were the most effective fungicides for detection of N. psidii in artificially infested soil or in naturally infected guava debris. Based on the requirement for carbon and nitrogen sources and response to fungicides, a semiselective medium designated as modified sucrose-glycine semiselective medium (mSGSSM) was developed for isolation of N. psidii, using the modified Czapek-Dox medium containing 3% sucrose, 0.3% glycine, iprodione at 5 μg ml–1, azoxystrobin at 1 μg ml–1, streptomycin at 200 μg ml–1, and neomycin at 200 μg ml–1. Colonies of N. psidii on mSGSSM at 30°C for 5 to 10 days were white to orange with sparse aerial hyphae. N. psidii was detected more accurately and efficiently on mSGSSM than on other media, including potato dextrose agar, modified Nash-Snyder medium, and modified Czapek-Dox medium. This semiselective medium is effective in detection of N. psidii from various parts of diseased guava trees and in soil; therefore, it would be a useful medium for etiological, ecological, and epidemiological studies of guava wilt.

The Screening of Optimal Ganoderma lucidum and the Effect of Medium Substrate on Bioactive Metabolite in Submerged Culture

2012 ◽  
Vol 554-556 ◽  
pp. 887-890
Author(s):  
Mei Lin Cui ◽  
Guo Qing He
Keyword(s):  
Yeast Extract ◽  
Ganoderma Lucidum ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
The Other ◽  
Carbon And Nitrogen Sources ◽  
Bioactive Metabolite ◽  
Carbon And Nitrogen ◽  
Other Hand

To determine an optimal G. lucidum strain, we made the production of mycelia biomass, EPS, IPS, IT, ET as indicators, among the five tested strains, the American G. lucidum was screened, and the production of mycelia biomass, EPS, IPS, IT, ET could reach 2.013g/100 ml, 11.2988 mg/100ml, 23.7800 mg/10ml, 45.5412 mg/100ml, 11.1417 mg/100ml, respectively. On the other hand, according to the screening of carbon and nitrogen sources as well as their concentration, the suitable carbon and nitrogen was 3% malt powder, 1.5% yeast extract. So we can use the fermentation culture for the following research, which was as follows：3% malt powder, 1.5%yeast extract, 0.3% KH2PO4, 0.15% MgSO4, 0.005% VB1.

scholarly journals Sıvı Kültürde Lepista Nuda Tarafından Miselyal Biyokütle ve Ekzopolisakkarit Üretimi İçin Karbon ve Azot Kaynaklarının Belirlenmesi

2018 ◽  
Vol 6 (5) ◽  
pp. 581
Author(s):  
Murat Özdal
Keyword(s):  
Biomass Production ◽  
Submerged Culture ◽  
Nitrogen Sources ◽  
Edible Mushroom ◽  
Mycelial Biomass ◽  
Carbon And Nitrogen Sources ◽  
Medium Carbon ◽  
Carbon And Nitrogen ◽  
Initial Medium ◽  
Optimized Conditions

In the present study, Lepista nuda, an edible mushroom, was used for the production of exopolysaccharide in the submerged culture. The effects of initial medium carbon and nitrogen sources on exopolysaccharide and mycelial biomass production by L. nuda IT strain were investigated. The highest exopolysaccharide production was obtained when culture parameters were used as initial medium 50 g/L glucose and 5 g/L yeast extract. As a result, 2.12 g/L exopolysaccharide and 12.3 g/L biomass production in optimized conditions at flask scales were achieved. This is the first study showing the production of exopolysaccharide in liquid culture of Lepista nuda.

The production of constitutive invertase and inulinase by the mushroom Panaeolus papillonaceus in submerged culture

1985 ◽  
Vol 31 (9) ◽  
pp. 773-777 ◽  
Author(s):  
Khana Mukherjee (Chakraborty) ◽  
S. Sengupta
Keyword(s):  
Submerged Culture ◽  
Agaricus Bisporus ◽  
Nitrogen Sources ◽  
Invertase Activity ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Inulinase Activity ◽  
Temperature Optima ◽  
Optimum Production ◽  
Starch Medium

Five different mushrooms were screened for extracellular inulinase and invertase activities. The enzymes were found to be produced constitutively by the mushrooms, but the carbon source, glucose, sucrose, and starch, in the medium influenced the production of individual enzymes. The highest producers of inulinase and invertase were Panaeolus papillonaceus growing in starch medium and Agaricus bisporus growing in glucose medium, respectively. A medium for the optimum production of invertase and inulinase by P. papillonaceus was developed by determining the optimum concentrations of suitable carbon and nitrogen sources. Some of the physicochemical properties of the enzymes present in the culture filtrate were studied. Both of the activities have pH optima at 6.5 and temperature optima at 60 °C. Km values for both enzyme activities were found to be identical at 2.5 mg of substrate/mL, whereas the νm for the inulinase activity was more than two times greater than that for invertase activity.

Factors influencing adhesive bonding at the bone/implant interface

1992 ◽  
Vol 50 (2) ◽  
pp. 1114-1115
Author(s):  
Julie A. Martini ◽  
Robert H. Doremus
Keyword(s):  
Electron Microscopy ◽  
Adhesive Bonding ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Bone Implant ◽  
Factors Influencing ◽  
Lr White ◽  
Transmission Electron ◽  
New Zealand White Rabbits ◽  
Scanning Electron

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

Transmission Electron Microscopy study of Bi-based superconducting phases

1990 ◽  
Vol 48 (4) ◽  
pp. 50-51
Author(s):  
J.G. Wen ◽  
K.K. Fung
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Single Crystals ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Space Groups ◽  
Superconducting Phases ◽  
Ceramic Samples ◽  
Transmission Electron ◽  
Modulated Structures

Bi-based superconducting phases have been found to be members of a structural series represented by Bi2Sr2Can−1Cun−1On+4, n=1,2,3, and are referred to as 2201, 2212, 2223 phases. All these phases are incommensurate modulated structures. The super space groups are P2/b, NBbmb 2201, 2212 phases respectively. Pb-doped ceramic samples and single crystals and Y-doped single crystals have been studied by transmission electron microscopy.Modulated structures of all Bi-based superconducting phases are in b-c plane, therefore, it is the best way to determine modulated structure and c parameter in diffraction pattern. FIG. 1,2,3 show diffraction patterns of three kinds of modulations in Pb-doped ceramic samples. Energy dispersive X-ray analysis (EDAX) confirms the presence of Pb in the three modulated structures. Parameters c are 3 0.06, 38.29, 30.24Å, ie 2212, 2223, 2212 phases for FIG. 1,2,3 respectively. Their average space groups are all Bbmb.

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