Co-inoculation withBacillussp. CECT 450 improves nodulation inPhaseolus vulgarisL.

2001 ◽  
Vol 47 (11) ◽  
pp. 1058-1062 ◽  
Author(s):  
M Camacho ◽  
C Santamaría ◽  
F Temprano ◽  
D N Rodriguez-Navarro ◽  
A Daza

The strain Bacillus sp. CECT 450 increased nodulation on bean (Phaseolus vulgaris L.) when co-inoculated with Rhizobium tropici CIAT 899. This positive effect occured under controlled conditions on perlite–vermiculite, sand, or in a mixture of soil and sand. This increase was also observed in a field assay. Nodulation kinetic studies suggested that the synergistic effect is pronounced during the latter stages of cultivation. In contrast, the same bacteria co-inoculated with Bradyrhizobium japonicum USDA 110 reduced nodulation on soybean (Glycine max (L.) Merr.). Inoculation with Bacillus sp. CECT 450 alone had no effect on bean plants, but reduced root growth in soybean. The survival of Bacillus sp. CECT 450 on inoculated seeds was high, even when inoculated seeds were maintained for several months at room temperature.Key words: Rhizobium spp., Bacillus sp., Phaseolus vulgaris, nodulation, co-inoculation.

2003 ◽  
Vol 27 (5) ◽  
pp. 833-840 ◽  
Author(s):  
L. M. O. Chueire ◽  
E. V. Bangel ◽  
F. L. Mostasso ◽  
R. J. Campo ◽  
F. O. Pedrosa ◽  
...  

As culturas da soja [Glycine max (L.) Merrill] e do feijoeiro (Phaseolus vulgaris L.) são de grande importância econômica e social para o Brasil e ambas podem ter seu requerimento de nitrogênio suprido pela simbiose com bactérias da ordem Rhizobiales. Para garantir a maximização do processo biológico, deve-se proceder à inoculação de estirpes de rizóbio eficientes e competitivas, recomendadas pela pesquisa. No Brasil, foram comercializados, na safra 2001/2002, 14 milhões de doses de inoculantes, dos quais 99 % para as culturas da soja e do feijoeiro. Neste trabalho, determinou-se a posição taxonômica das estirpes utilizadas em inoculantes comerciais para as duas culturas, pelo seqüenciamento da região do DNA que codifica o gene 16S rRNA, que é suficientemente variável, mas carrega as informações necessárias para permitir a análise filogenética de bactérias. O seqüenciamento permitiu definir que duas das estirpes recomendadas para a cultura da soja, SEMIA 587 e SEMIA 5019 (= 29 w), pertencem à espécie Bradyrhizobium elkanii e as duas outras, SEMIA 5079 (=CPAC 15) e SEMIA 5080 (= CPAC 7), à espécie B. japonicum. Determinou-se, ainda, que a estirpe SEMIA 4080 (=PRF 81), recomendada para o cultura do feijoeiro, pertence à espécie Rhizobium tropici. As seqüências obtidas foram depositadas no banco mundial de genes do National Center for Biotechnology Information.


2019 ◽  
Vol 20 (9) ◽  
pp. 2327 ◽  
Author(s):  
Marcello Iriti ◽  
Alessio Scarafoni ◽  
Simon Pierce ◽  
Giulia Castorina ◽  
Sara Vitalini

EM (effective microorganisms) is a biofertilizer consisting of a mixed culture of potentially beneficial microorganisms. In this study, we investigated the effects of EM treatment on leaf in vivo chlorophyll a fluorescence of photosystem II (PSII), yield, and macronutrient content of bean plants grown on different substrates (nutrient rich substrate vs. nutrient poor sandy soil) in controlled environmental conditions (pot experiment in greenhouse). EM-treated plants maintained optimum leaf photosynthetic efficiency two weeks longer than the control plants, and increased yield independent of substrate. The levels of seed nutritionally-relevant molecules (proteins, lipids, and starch) were only slightly modified, apart from the protein content, which increased in plants grown in sandy soil. Although EM can be considered a promising and environmentally friendly technology for sustainable agriculture, more studies are needed to elucidate the mechanism(s) of action of EM, as well as its efficacy under open field conditions.


2014 ◽  
Vol 10 (2) ◽  
pp. 283-293
Author(s):  
Barbara Łacicowa ◽  
Zofia Machowicz

The results obtained in pot and field experiments have shown that <i>Helminthosporium sorokinianum</i> is able to infect bean plants. The cotyledons and roots of shoots during the first three weeks of growth are attacked the most frequently. Dark brown spots occur on the above-mentined organs. The infection of roots and cotyledonsof shoots is responsible for gangrene both before and after germination. Infected plants which remain alive only show symptoms of infection in the root system. The infection of roots by <i>H. sorokinianum</i> in older plants is detromental to growth and causes a decrease in the vield obtained from bean plants.


1950 ◽  
Vol 1 (2) ◽  
pp. 148 ◽  
Author(s):  
CG Greenham

As determined by the length for which roots were killed, there was no diurnal variation in the effectiveness on skeleton weed (Chondrilla juncea L.) of spray applications of 'Methoxone' at two-hourly intervals over a period of 24 hours. As determined by reduction in growth above the primary leaves, there was a well-marked diurnal variation in the effectiveness on bean plants (Phaseolus vulgaris L.) of drop applications of 'Methoxone.'


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