Genetic diversity ofSinorhizobiumpopulations recovered from differentMedicagovarieties cultivated in Tunisian soils

2001 ◽  
Vol 47 (2) ◽  
pp. 139-147 ◽  
Author(s):  
M Jebara ◽  
R Mhamdi ◽  
M E Aouani ◽  
R Ghrir ◽  
M Mars
Keyword(s):  
Sinorhizobium Meliloti ◽  
Dna Analysis ◽  
Rflp Analysis ◽  
Plasmid Profile ◽  
Enzyme Electrophoresis ◽  
Phenotypic Properties ◽  
Sinorhizobium Medicae ◽  
Pcr Rflp ◽  
Medicago Ciliaris ◽  
Inoculation Group

A collection of 468 rhizobial isolates was obtained from different ecological areas of Tunisia by trapping them on Medicago sativa cv. Gabes, Medicago scutelleta cv. Kelson, Medicago truncatula, and Medicago ciliaris. A subsample of 134 rhizobia was chosen to determine their plasmid profile, and 89 isolates were subjected to multilocus enzyme electrophoresis (MLEE) and PCR/RFLP analysis using 16S, IGS (inter genic spacer), and nifKD probes. Twenty-five representatives from these isolates were evaluated for their nodulation and nitrogen fixation capacities. MLEE studies revealed two groups with highly heterogeneous host specificity and geographical origin. The discriminatory power was found to be slightly better with the amplified ribosomal intergenic region, than the nifKD genes. Divisions detected by nifKD amplified DNA analysis matched those established by ribosomal PCR- RFLPs. The comparison between different analyses revealed that MLEE illustrated better phenotypic properties of isolates than PCR-RFLP or plasmid content analysis. Clear distinction between Sinorhizobium meliloti and Sinorhizobium medicae were observed by analysis of the IGS symbiotic regions between nifD and nifK genes. Were able to distinguish three inoculation groups; isolates trapped from M. sativa cv. Gabes and M. scutelleta cv. Kelson formed one inoculation group which was more closely related to isolates trapped from M. truncatula than those trapped from M. ciliaris.Key words: Sinorhizobium, Medicago, diversity, MLEE, PCR, symbiotic effectiveness.

Medicago ciliaris growing in Tunisian soils is preferentially nodulated by Sinorhizobium medicae

Soil Research ◽  
2007 ◽  
Vol 45 (6) ◽  
pp. 473 ◽  
Author(s):  
K. Zribi ◽  
Y. Badri ◽  
S. Saidi ◽  
P. van Berkum ◽  
M. E. Aouani
Keyword(s):  
Nitrogen Fixation ◽  
16S Rrna ◽  
Plant Growth ◽  
Soil Types ◽  
Inoculation Experiment ◽  
Climatic Zones ◽  
Nodule Number ◽  
Sinorhizobium Medicae ◽  
Pcr Rflp ◽  
Medicago Ciliaris

Variation in growth of Medicago ciliaris was recorded across soils from 5 different regions in Tunisia that represented different soil types and climatic zones. In 4 of these soils (Mateur, Enfidha, Rhayet, and Soliman) this variation appeared to be related to the nodule number on the roots of the plants. With the exception of one isolate the rhizobia isolated from these nodules had 16S rRNA PCR-RFLP fingerprint patterns that were characteristic of Sinorhizobium medicae. Plant growth in the fifth soil (Jelma) was the poorest; plants had few nodules that yielded exclusively rhizobia with 16S rRNA fingerprint patterns characteristic of S. meliloti. In subsequent plant tests, S. medicae isolates formed effective nitrogen fixation symbioses with M. ciliaris, while S. meliloti formed small, white, ineffective nodules. Therefore, plant growth in Jelma soil was poor because only S. meliloti are present and this species is ineffective with M. ciliaris. In a co-inoculation experiment with M. ciliaris, S. medicae was more competitive for nodulation than S. meliloti, perhaps explaining why the majority of the isolates from Enfidha and Rhayet were S. medicae, since S. meliloti is present in these soils. However, it is not clear how the host influences rhizobia for nodulation by S. medicae in preference to S. meliloti when present.

Distinct genetic profiles of Leishmania (Viannia) braziliensis associate with clinical variations in cutaneous-leishmaniasis patients from an endemic area in Brazil

Parasitology ◽  
2018 ◽  
Vol 145 (9) ◽  
pp. 1161-1169 ◽  
Author(s):  
Patrícia Flávia Quaresma ◽  
Cristiana Ferreira Alves de Brito ◽  
Jeronimo Marteleto Nunes Rugani ◽  
Janaína de Moura Freire ◽  
Rodrigo de Paula Baptista ◽  
...  
Keyword(s):  
Clinical Manifestations ◽  
Rflp Analysis ◽  
Control Measures ◽  
Clinical Samples ◽  
Genetic Profile ◽  
Leishmania Braziliensis ◽  
Enzyme Electrophoresis ◽  
Restriction Profile ◽  
Pcr Rflp ◽  
American Tegumentary Leishmaniasis

AbstractAmerican tegumentary leishmaniasis (ATL) samples obtained from the lesions of patients with typical (n = 25, 29%), atypical (n = 60, 69%) or both (n = 2%) clinical manifestations were analysed by multilocus enzyme electrophoresis, hsp70 restriction-fragment length polymorphism (PCR-RFLP), hsp70 sequencing and phylogenetics methods. The hsp70 PCR-RFLP analysis revealed two different profiles whose the most samples differed from those expected for Leishmania braziliensis and the other Leishmania species tested: of 39 samples evaluated, two (5%) had a restriction profile corresponding to L. braziliensis, and 37 (95%) had a restriction profile corresponding to a variant pattern. A 1300-bp hsp70 gene fragment was sequenced to aid in parasite identification and a phylogenetic analysis was performed including 26 consensus sequences from the ATL patient's samples and comparing to other Leishmania and trypanosomatids species. The dendrogram allowed to observe a potential population structure of L. braziliensis complex in the studied region, emphasizing that the majority of clinical samples presented a variant genetic profile. Of interest, the L. braziliensis diversity was associated with different clinical manifestations whose parasites with hsp70 variant profile were associated with atypical lesions. The results may be helpful to improve the diagnosis, treatment and control measures of the ATL in endemic areas.

Molecular authentication of Thai medicinal plant Khamin khruea by PCR-RFLP analysis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
P Rojsanga ◽  
W Gritsanapan ◽  
W Leelamanit ◽  
S Sukrong
Keyword(s):  
Medicinal Plant ◽  
Rflp Analysis ◽  
Molecular Authentication ◽  
Pcr Rflp

Genetic and Biochemical Markers of Ovarian Function and Their Impact on Women Reproductive Status

2020 ◽  
Vol 71 (10) ◽  
pp. 212-217
Author(s):  
Adina-Elena Tanase ◽  
Roxana Popescu ◽  
Mircea Onofriescu ◽  
Roxana Daniela Matasariu
Keyword(s):  
Ovarian Function ◽  
Rflp Analysis ◽  
Fsh Receptor ◽  
Gene Encoding ◽  
Female Population ◽  
Fshr Gene ◽  
Pcr Rflp ◽  
Regulation Mechanisms ◽  
American Society ◽  
Dependent Mechanism

Endometriosis is a disease very common nowadays affecting 1-2% of the female population, by estrogen-dependent mechanism. The identification of mutations in the gene encoding for the FSH receptor (FSHR) has been reported since 1995. Physiology teaches us that follicle-stimulating hormone (FSH) is a hormone that is vital in the steroidogenesis regulation mechanisms, while FSH receptor (FSHR) activation helps to promote folliculogenesis and estrogensynthesis. Therefore, studies to show if there are any correlations between endometriosis and FSHR are acquired. Genotyping of FSHR gene polymorphisms were performed using PCR - Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. We analysed a total of 78 patients, 44 infertile patients with endometriosis and 34 controls (non-infertile, pregnant patients). The endometriosis group included women with diagnosis of endo-metriosis confirmed by laparoscopy and /or laparotomy and histological evidence of disease with the endometriosis staging according to American Society for Reproductive Medicine (ASRM). Corroborated with the severity of endometriosis, A919G and A2039G tests found that 71.4% of the M (GG) results were associated with primary infertility, not statistically significant (p=0.994) and 42.9% of the total M results had moderate or severe forms of endometriosis (p = 0.185). The genetic involvement in different pathologies such as endometriosis, has yet to be understood, but knowing more about its mechanism, will help physician target the disease at a more profound level.

Sign in / Sign up

Export Citation Format

Share Document