scholarly journals Genetic diversity of rhizobia nodulating Trifolium ambiguum in North America

2001 ◽  
Vol 47 (1) ◽  
pp. 81-85 ◽  
Author(s):  
P Seguin ◽  
P H Graham ◽  
C C Sheaffer ◽  
N J Ehlke ◽  
M P Russelle
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
North American ◽  
Diversity Index ◽  
Diversity Indices ◽  
Clustering Methods ◽  
Center Of Origin ◽  
Trifolium Ambiguum ◽  
Kura Clover ◽  
The North

Kura clover (Trifolium ambiguum M.B.) is a persistent rhizomatous forage legume, whose use in the U.S.A. is limited by establishment difficulties in part attributable to nodulation problems. In this study, soil was collected from established stands of Kura clover growing in 9 diverse North American environments. Rhizobia were plant-trapped using Kura clover cv. Endura as host, then rhizobia from nodules fingerprinted using BOX-PCR. The diversity of isolates from North America was then contrasted to that of rhizobia from a single Caucasian environment (Russia), the center of origin for this species. Populations were characterized using clustering methods, and genetic diversity estimated using the Shannon-Weaver diversity index. The genetic diversity of the North American populations was extremely limited, all isolates being closely related to two of the strains found in a locally available commercial inoculant. In contrast, Russian isolates formed a distinct cluster with significant internal genetic diversity. Genetic diversity indices for the North American and Russian populations were 3.5 and 10.76, respectively. The implication of this and other studies is that Kura clover is highly specific in Rhizobium requirement. If the performance of this legume in the U.S.A. is to be improved, either by modifying current establishment practices or plant breeding, it is essential that these studies be paralleled by more collections and evaluation of rhizobia from its center of origin, given the extremely limited diversity of rhizobia found in North America.Key words: genetic diversity, rhizobia, Kura clover, BOX-PCR.

scholarly journals Phylogenetics of a Fungal Invasion: Origins and Widespread Dispersal of White-Nose Syndrome

mBio ◽  
2017 ◽  
Vol 8 (6) ◽  
Author(s):  
Kevin P. Drees ◽  
Jeffrey M. Lorch ◽  
Sebastien J. Puechmaille ◽  
Katy L. Parise ◽  
Gudrun Wibbelt ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
North American ◽  
Fungal Pathogen ◽  
Phylogenetic Study ◽  
Pseudogymnoascus Destructans ◽  
Long Distance ◽  
Recent Introduction ◽  
White Nose Syndrome ◽  
The North

ABSTRACT Globalization has facilitated the worldwide movement and introduction of pathogens, but epizoological reconstructions of these invasions are often hindered by limited sampling and insufficient genetic resolution among isolates. Pseudogymnoascus destructans , a fungal pathogen causing the epizootic of white-nose syndrome in North American bats, has exhibited few genetic polymorphisms in previous studies, presenting challenges for both epizoological tracking of the spread of this fungus and for determining its evolutionary history. We used single nucleotide polymorphisms (SNPs) from whole-genome sequencing and microsatellites to construct high-resolution phylogenies of P. destructans . Shallow genetic diversity and the lack of geographic structuring among North American isolates support a recent introduction followed by expansion via clonal reproduction across the epizootic zone. Moreover, the genetic relationships of isolates within North America suggest widespread mixing and long-distance movement of the fungus. Genetic diversity among isolates of P. destructans from Europe was substantially higher than in those from North America. However, genetic distance between the North American isolates and any given European isolate was similar to the distance between the individual European isolates. In contrast, the isolates we examined from Asia were highly divergent from both European and North American isolates. Although the definitive source for introduction of the North American population has not been conclusively identified, our data support the origin of the North American invasion by P. destructans from Europe rather than Asia. IMPORTANCE This phylogenetic study of the bat white-nose syndrome agent, P. destructans , uses genomics to elucidate evolutionary relationships among populations of the fungal pathogen to understand the epizoology of this biological invasion. We analyze hypervariable and abundant genetic characters (microsatellites and genomic SNPs, respectively) to reveal previously uncharacterized diversity among populations of the pathogen from North America and Eurasia. We present new evidence supporting recent introduction of the fungus to North America from a diverse Eurasian population, with limited increase in genetic variation in North America since that introduction.

scholarly journals Strong Genetic Differentiation Between North American and European Populations of Phytophthora alni subsp. uniformis

2013 ◽  
Vol 103 (2) ◽  
pp. 190-199 ◽  
Author(s):  
Jaime Aguayo ◽  
Gerard C. Adams ◽  
Fabien Halkett ◽  
Mursel Catal ◽  
Claude Husson ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
North America ◽  
Mating System ◽  
North American ◽  
Parental Species ◽  
European Countries ◽  
North American Population ◽  
Natural Ecosystems ◽  
Reproduction Mode ◽  
The North

Alder decline caused by Phytophthora alni has been one of the most important diseases of natural ecosystems in Europe during the last 20 years. The emergence of P. alni subsp. alni —the pathogen responsible for the epidemic—is linked to an interspecific hybridization event between two parental species: P. alni subsp. multiformis and P. alni subsp. uniformis. One of the parental species, P. alni subsp. uniformis, has been isolated in several European countries and, recently, in North America. The objective of this work was to assess the level of genetic diversity, the population genetic structure, and the putative reproduction mode and mating system of P. alni subsp. uniformis. Five new polymorphic microsatellite markers were used to contrast both geographical populations. The study comprised 71 isolates of P. alni subsp. uniformis collected from eight European countries and 10 locations in North America. Our results revealed strong differences between continental populations (Fst = 0.88; Rst = 0.74), with no evidence for gene flow. European isolates showed extremely low genetic diversity compared with the North American collection. Selfing appears to be the predominant mating system in both continental collections. The results suggest that the European P. alni subsp. uniformis population is most likely alien and derives from the introduction of a few individuals, whereas the North American population probably is an indigenous population.

scholarly journals Progress in Diagnosing Primary Ciliary Dyskinesia: The North American Perspective

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1278
Author(s):  
Michael Glenn O’Connor ◽  
Amjad Horani ◽  
Adam J. Shapiro
Keyword(s):  
Genetic Testing ◽  
North America ◽  
North American ◽  
Primary Ciliary Dyskinesia ◽  
Clinical Phenotype ◽  
The United States ◽  
Diagnostic Process ◽  
The North ◽  
Diagnostic Guideline ◽  
Ciliary Dyskinesia

Primary Ciliary Dyskinesia (PCD) is a rare, under-recognized disease that affects respiratory ciliary function, resulting in chronic oto-sino-pulmonary disease. The PCD clinical phenotype overlaps with other common respiratory conditions and no single diagnostic test detects all forms of PCD. In 2018, PCD experts collaborated with the American Thoracic Society (ATS) to create a clinical diagnostic guideline for patients across North America, specifically considering the local resources and limitations for PCD diagnosis in the United States and Canada. Nasal nitric oxide (nNO) testing is recommended for first-line testing in patients ≥5 years old with a compatible clinical phenotype; however, all low nNO values require confirmation with genetic testing or ciliary electron micrograph (EM) analysis. Furthermore, these guidelines recognize that not all North American patients have access to nNO testing and isolated genetic testing is appropriate in cases with strong clinical PCD phenotypes. For unresolved diagnostic cases, referral to a PCD Foundation accredited center is recommended. The purpose of this narrative review is to provide insight on the North American PCD diagnostic process, to enhance the understanding of and adherence to current guidelines, and to promote collaboration with diagnostic pathways used outside of North America.

New multituberculate mammal from the Early Cretaceous of eastern North America

2013 ◽  
Vol 50 (3) ◽  
pp. 315-323 ◽  
Author(s):  
Richard L. Cifelli ◽  
Cynthia L. Gordon ◽  
Thomas R. Lipka
Keyword(s):  
North America ◽  
Iberian Peninsula ◽  
Early Cretaceous ◽  
North American ◽  
Lower Cretaceous ◽  
Late Jurassic ◽  
Relative Length ◽  
Central Position ◽  
Eastern North America ◽  
The North

Multituberculates, though among the most commonly encountered mammalian fossils of the Mesozoic, are poorly known from the North American Early Cretaceous, with only one taxon named to date. Herein we describe Argillomys marylandensis, gen. et sp. nov., from the Early Cretaceous of Maryland, based on an isolated M2. Argillomys represents the second mammal known from the Arundel Clay facies of the Patuxent Formation (Lower Cretaceous: Aptian). Though distinctive in its combination of characters (e.g., enamel ornamentation consisting of ribs and grooves only, cusp formula 2:4, presence of distinct cusp on anterobuccal ridge, enlargement of second cusp on buccal row, central position of ultimate cusp in lingual row, great relative length), the broader affinities of Argillomys cannot be established because of non-representation of the antemolar dentition. Based on lack of apomorphies commonly seen among Cimolodonta (e.g., three or more cusps present in buccal row, fusion of cusps in lingual row, cusps strongly pyramidal and separated by narrow grooves), we provisionally regard Argillomys as a multituberculate of “plagiaulacidan” grade. Intriguingly, it is comparable in certain respects to some unnamed Paulchoffatiidae, a family otherwise known from the Late Jurassic – Early Cretaceous of the Iberian Peninsula.

A Kinematic Reconstruction of Jurassic ocean spreading from the ophiolites of California, the western U.S. using structural geology and paleomagnetism.

2021 ◽  
Author(s):  
Cemil Arkula ◽  
Nalan Lom ◽  
John Wakabayashi ◽  
Grant Rea-Downing ◽  
Mark Dekkers ◽  
...  
Keyword(s):  
North America ◽  
Relative Position ◽  
North American ◽  
Fault System ◽  
Paleomagnetic Data ◽  
Geochemical Data ◽  
Ophiolite Belt ◽  
The North ◽  
Western Us ◽  
Jurassic Ophiolites

<p>The western edge of the North America plate contains geological records that formed during the long-lived convergence between plates of the Panthalassa Ocean and North America. The geology of different segments along western North America indicates different polarities (eastward and westward) for subducted slabs and thereby various tectonic histories and settings. The western United States (together with Mexico) plays a key role in this debate, many geologic interpretations assume continuous eastward subduction in contrast to observations within proximal geologic segments and tomographic images of the lower mantle below North America and the eastern Pacific Ocean which suggest a more complex subduction history. In this study, we aim to evaluate the plate tectonic setting in which the Jurassic ophiolites of California formed. Geochemical data from these ophiolites suggest that they formed above a nascent intra-oceanic or continental margin subduction zone. We first developed a kinematic reconstruction of the western US geology back to the Jurassic based on published structural geological data. Importantly, we update the reconstruction of the various branches of the San Andreas fault system to determine the relative position of the ophiolite fragments and adopt a previous restoration of Basin and Range extension which we expand northward towards Washington state. We then reconstruct North American margin deformation associated with Cretaceous to Paleogene shortening and strike-slip faulting. We find no clear candidates in the geological record that may have accommodated major subduction between the Jurassic ophiolite belt and the North American margin and consequently concur with the school of thought that considers that the ophiolite belt, as well as the underlying subduction-accretionary Franciscan Complex, likely formed in the North American fore-arc. We collected paleomagnetic data to reconstruct the spreading direction of the Jurassic Californian ophiolites, by providing new paleomagnetic data from sheeted dykes of the Josephine and Mt. Diablo Ophiolites. These suggest a NE-SW paleo-ridge orientation, oblique to the North American margin which may be explained by partitioning of a dextral component of subduction obliquity relative to North America. We used this spreading direction in combination with published ages of the ophiolites and our restoration of the relative position of these ophiolites prior to post-Jurassic deformation to construct a ridge-transform system at which the Jurassic ophiolites accreted. The results will be used to evaluate which parts of the subduction systems that existed in the eastern Panthalassa Ocean may reside in the western US, and which parts may be better sought in the northern Canadian Segment or/and in the southern Caribbean region.</p>

Geographic patterns of genetic diversity in North American trees: a continent-wide analysis

2021 ◽  
Author(s):  
Andrew V. Gougherty
Keyword(s):  
Genetic Diversity ◽  
Population Genetics ◽  
North America ◽  
North American ◽  
Tree Species ◽  
Geographic Patterns ◽  
Study Characteristics ◽  
Dispersal Traits ◽  
The Last Glacial Maximum ◽  
The Last Glacial

In the northern hemisphere, many species have been reported to have greater genetic diversity in southern populations than northern populations - ostensibly due to migration northward following the last glacial maximum (LGM). The generality of this pattern, while well-established for some taxa, remains unclear for North American trees. To address this issue, I collected published population genetics data for 73 North American tree species, and tested whether genetic diversity was associated with latitude or longitude and whether geographic trends were associated with dispersal traits, range or study characteristics. I found there were no general geographic patterns in genetic diversity, and the strength of the geographic gradients were not associated with any species or study characteristics. Species in the northern and western regions of North America tended to have more species with genetic diversity that declined with latitude, but most species had no significant trend. This work shows that North American trees have complex, individualistic, patterns of genetic diversity that may negate explanation by any particular dispersal trait or range characteristic.

scholarly journals Contrastive Influence of ENSO and PNA on Variability and Predictability of North American Winter Precipitation

2019 ◽  
Vol 32 (19) ◽  
pp. 6271-6284 ◽  
Author(s):  
Xiaofan Li ◽  
Zeng-Zhen Hu ◽  
Ping Liang ◽  
Jieshun Zhu
Keyword(s):  
North America ◽  
Surface Temperature ◽  
Sea Surface Temperature ◽  
North American ◽  
North Pacific ◽  
Winter Precipitation ◽  
Sea Surface ◽  
The North ◽  
Remote Forcing ◽  
The North Pacific

Abstract In this work, the roles of El Niño–Southern Oscillation (ENSO) in the variability and predictability of the Pacific–North American (PNA) pattern and precipitation in North America in winter are examined. It is noted that statistically about 29% of the variance of PNA is linearly linked to ENSO, while the remaining 71% of the variance of PNA might be explained by other processes, including atmospheric internal dynamics and sea surface temperature variations in the North Pacific. The ENSO impact is mainly meridional from the tropics to the mid–high latitudes, while a major fraction of the non-ENSO variability associated with PNA is confined in the zonal direction from the North Pacific to the North American continent. Such interferential connection on PNA as well as on North American climate variability may reflect a competition between local internal dynamical processes (unpredictable fraction) and remote forcing (predictable fraction). Model responses to observed sea surface temperature and model forecasts confirm that the remote forcing is mainly associated with ENSO and it is the major source of predictability of PNA and winter precipitation in North America.

scholarly journals Novel Highly Pathogenic Avian A(H5N2) and A(H5N8) Influenza Viruses of Clade 2.3.4.4 from North America Have Limited Capacity for Replication and Transmission in Mammals

mSphere ◽  
2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Bryan S. Kaplan ◽  
Marion Russier ◽  
Trushar Jeevan ◽  
Bindumadhav Marathe ◽  
Elena A. Govorkova ◽  
...  
Keyword(s):  
North America ◽  
Avian Influenza ◽  
North American ◽  
Influenza Viruses ◽  
Morbidity And Mortality ◽  
Avian Influenza Viruses ◽  
Highly Pathogenic ◽  
The North ◽  
Domestic Poultry ◽  
The Impact

ABSTRACT Highly pathogenic H5 influenza viruses have been introduced into North America from Asia, causing extensive morbidity and mortality in domestic poultry. The introduced viruses have reassorted with North American avian influenza viruses, generating viral genotypes not seen on other continents. The experiments and analyses presented here were designed to assess the impact of this genetic diversification on viral phenotypes, particularly as regards mammalian hosts, by comparing the North American viruses with their Eurasian precursor viruses. Highly pathogenic influenza A(H5N8) viruses from clade 2.3.4.4 were introduced to North America by migratory birds in the fall of 2014. Reassortment of A(H5N8) viruses with avian viruses of North American lineage resulted in the generation of novel A(H5N2) viruses with novel genotypes. Through sequencing of recent avian influenza viruses, we identified PB1 and NP gene segments very similar to those in the viruses isolated from North American waterfowl prior to the introduction of A(H5N8) to North America, highlighting these bird species in the origin of reassortant A(H5N2) viruses. While they were highly virulent and transmissible in poultry, we found A(H5N2) viruses to be low pathogenic in mice and ferrets, and replication was limited in both hosts compared with those of recent highly pathogenic avian influenza (HPAI) H5N1 viruses. Molecular characterization of the hemagglutinin protein from A(H5N2) viruses showed that the receptor binding preference, cleavage, and pH of activation were highly adapted for replication in avian species and similar to those of other 2.3.4.4 viruses. In addition, North American and Eurasian clade 2.3.4.4 H5NX viruses replicated to significantly lower titers in differentiated normal human bronchial epithelial cells than did seasonal human A(H1N1) and highly pathogenic A(H5N1) viruses isolated from a human case. Thus, despite their having a high impact on poultry, our findings suggest that the recently emerging North American A(H5N2) viruses are not expected to pose a substantial threat to humans and other mammals without further reassortment and/or adaptation and that reassortment with North American viruses has not had a major impact on viral phenotype. IMPORTANCE Highly pathogenic H5 influenza viruses have been introduced into North America from Asia, causing extensive morbidity and mortality in domestic poultry. The introduced viruses have reassorted with North American avian influenza viruses, generating viral genotypes not seen on other continents. The experiments and analyses presented here were designed to assess the impact of this genetic diversification on viral phenotypes, particularly as regards mammalian hosts, by comparing the North American viruses with their Eurasian precursor viruses.

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