Structural characterization of the O-chain polysaccharide from Proteusmirabilis strain 7570

1995 ◽  
Vol 73 (10) ◽  
pp. 1600-1604 ◽  
Author(s):  
Dušan Uhrín ◽  
Vandana Chandan ◽  
Eleonora Altman
Keyword(s):  
Molecular Weight ◽  
Nmr Spectroscopy ◽  
Chemical Analysis ◽  
High Molecular Weight ◽  
Structural Characterization ◽  
Galacturonic Acid ◽  
2D Nmr ◽  
Chain Structure ◽  
2D Nmr Spectroscopy

The O-chain polysaccharide produced by Proteusmirabilis strain 7570 was shown by chemical analysis and 1D and 2D NMR spectroscopy to be a high molecular weight acidic linear polymer of tetrasaccharide repeating units, composed of D-galacturonic acid, 2-acetamido-2-deoxy-D-galactose, and 2-acetamido-2-deoxy-D-glucose (2:1:1). In addition, native O-chain was randomly substituted by O-acetyl groups. Keywords: Proteus, lipopolysaccharide, O-chain, structure, NMR.

Chemical constituents from Penianthus camerounensis Dekker (Menispermaceae)

2019 ◽  
Vol 74 (10) ◽  
pp. 703-708 ◽  
Author(s):  
Georges Bellier Tabekoueng ◽  
Carine Mvot Akak ◽  
Moses K. Langat ◽  
Anatole Guy Blaise Azebaze ◽  
Alain François Kamdem Waffo ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Chemical Analysis ◽  
Betulinic Acid ◽  
Chemical Constituents ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Phytochemical Study ◽  
Chemical Structures ◽  
First Time

AbstractThe phytochemical study of the roots, leaves and twigs of Penianthus camerounensis Dekker (Menispermaceae) has led to the isolation and the characterization of 20 compounds. A ceramide, camerounamide (1), and a furoclerodanediterpenoid, camerounin (2), have not been described previously, while the compounds xylopic acid (3), syringaresinol (4), iso-propylmethylcyclohexa-1,4-diol (5), 1-(28-hydroxyoctacosanoyl)glycerol (6), scoparone (7), friedelin (8), friedelanol (9) and betulinic acid (10) are being reported for the first time from the genus Penianthus alongside 10 known compounds (11–20). Chemical structures were determined using 1D- and 2D-NMR spectroscopy, MS and chemical analysis. Their chemotaxonomic importance is discussed.

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

scholarly journals Secondary Metabolites from the Roots of Salvia Palaestina Bentham

2008 ◽  
Vol 3 (12) ◽  
pp. 1934578X0800301 ◽  
Author(s):  
Antonio Vassallo ◽  
Ammar Bader ◽  
Alessandra Braca ◽  
Angela Bisio ◽  
Luca Rastrelli ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Secondary Metabolites ◽  
Chemical Analysis ◽  
2D Nmr ◽  
2D Nmr Spectroscopy

Two new sesquiterpenes (1–2), and one diterpene (3) were isolated from the roots of Salvia palaestina Bentham (Lamiaceae), together with eight known diterpenes and two triterpenes. Their structures were elucidated by 1D and 2D NMR spectroscopy, including 1D-TOCSY, DQF-COSY, ROESY, HSQC, and HMBC experiments, as well as ESIMS and chemical analysis.

Structural Characterization of High Molecular Weight Starch Granule-Bound Proteins in Wheat (Triticum aestivumL.)

1997 ◽  
Vol 45 (8) ◽  
pp. 2929-2934 ◽  
Author(s):  
Motoko Takaoka ◽  
Shogo Watanabe ◽  
Hidenori Sassa ◽  
Makoto Yamamori ◽  
Toshiki Nakamura ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Structural Characterization ◽  
Starch Granule
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