Identification of 2-deoxy-D-ribono-1,4-lactone at the site of benzophenone photosensitized release of guanine in 2′-deoxyguanosine and thymidylyl-(3′-5′)-2′-deoxyguanosine

1992 ◽  
Vol 70 (6) ◽  
pp. 1827-1832 ◽  
Author(s):  
Garry W. Buchko ◽  
Jean Cadet
Keyword(s):  
Aqueous Solution ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Proton Magnetic Resonance ◽  
Proton Magnetic Resonance Spectroscopy ◽  
Fast Atom Bombardment ◽  
Resonance Spectroscopy ◽  
Coupling Constant ◽  
Reverse Phase ◽  
Dinucleoside Monophosphates

Benzophenone-mediated photosensitization of 2′-deoxyguanosine (dG) and thymidylyl-(3′-5′)-2′-deoxyguanosine (d(TpG)) at 350 nm in oxygen-saturated aqueous solution produces 2-deoxy-D-ribono-1,4-lactone (dL) and thymidylyl-(3′-5′)-2-deoxy-D-ribono-1,4-lactone (d(TpL)), respectively. These photoproducts have been isolated by reverse phase high-performance liquid chromatography and characterized by proton magnetic resonance spectroscopy and fast atom bombardment mass spectroscopy. Conformational analysis of d(TpG) and d(TpL) in aqueous solution using coupling constant data suggests no major conformational differences between the two dinucleoside monophosphates.

scholarly journals Altered peptidoglycan composition in vancomycin-resistant Enterococcus faecalis.

1996 ◽  
Vol 40 (4) ◽  
pp. 863-869 ◽  
Author(s):  
B L de Jonge ◽  
S Handwerger ◽  
D Gage
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Enterococcus Faecalis ◽  
Resistant Strain ◽  
High Performance ◽  
Fast Atom Bombardment ◽  
Vancomycin Resistant Enterococcus ◽  
Reverse Phase ◽  
Vancomycin Resistant

The muropeptide compositions of isogenic vancomycin-resistant and -susceptible Enterococcus faecalis strains were analyzed by reverse-phase high-performance liquid chromatography combined with amino acid analysis and fast atom bombardment mass spectrometry. Peptidoglycan of the susceptible strain contained pentapeptides as stem peptides, whereas peptidoglycan of the isogenic resistant strain was composed of muropeptides with tetrapeptide stem peptides. Despite the synthesis of lactate-terminating peptidoglycan precursors, no lactate-containing muropeptides were detected in peptidoglycan of the resistant strain. These findings indicate that either lactate-terminating precursors are not incorporated into peptidoglycan of the resistant strain or that the lactate residues are removed from peptidoglycan during synthesis.

scholarly journals Improvement of Water Solubility of Mercaptoundecahydrododecaborate (BSH)-Peptides by Conjugating with Ethylene Glycol Linker and Interaction with Cyclodextrin

Processes ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 167
Author(s):  
Mizuki Kitamatsu ◽  
Ayaka Nakamura-Tachibana ◽  
Yoshimichi Ishikawa ◽  
Hiroyuki Michiue
Keyword(s):  
Aqueous Solution ◽  
High Performance Liquid Chromatography ◽  
Ethylene Glycol ◽  
Neutron Capture ◽  
High Performance ◽  
Water Solubility ◽  
Neutron Capture Therapy ◽  
Reverse Phase ◽  
Boron Neutron Capture ◽  
Chain Lengths

We previously developed a conjugate consisting of 10B cluster BSH and tri-arginine peptide (BSH-3R). This could potentially be used as a boron agent for boron neutron capture therapy; however, it possesses poor water solubility and thus needs to be improved for use as medicine. In this study, we devised several means of improving the water solubility of BSH-3R. As one of them, we used cyclodextrin (CD), which was expected to improve the water solubility resulting from interaction of the BSH-3R with CD. We evaluated the solubility of BSH-3R in aqueous CD solution by using reverse-phase high-performance liquid chromatography. As we expected, the solubility of BSH-3R was increased in a manner dependent on the addition of β-CD and γ-CD in aqueous solution. Furthermore, we synthesized BSH conjugated to oligoarginine having various chain lengths (BSH-nR) and BSH-3R with ethylene glycol linkers introduced between BSH and 3R (BSH-nEg-3R). The water solubility of these BSH peptides was also evaluated and the results showed that the introduction of nEg to BSH-3R markedly improved the water solubility. Furthermore, we found that the water solubility of these peptides can be further improved by also applying CD.

N.M.R. studies of aqua exchange on trimethylaquabipyridineplatinum(IV)

1970 ◽  
Vol 23 (10) ◽  
pp. 1981 ◽  
Author(s):  
DE Clegg ◽  
JR Hall ◽  
NS Ham
Keyword(s):  
Aqueous Solution ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Proton Magnetic Resonance ◽  
Proton Magnetic Resonance Spectroscopy ◽  
Proton Spectrum ◽  
Water Ligand ◽  
Resonance Spectroscopy ◽  
Methyl Proton ◽  
Coordinated Water

Proton magnetic resonance spectroscopy has been used to study the exchange reaction which is responsible in aqueous solution for averaging the two methyl proton signals of trimethylaquabipyridineplatinum(1V) cation. The dependence of the rate upon cation concentration, pH, added sulphate ion, and temperature and the appearance of 195Pt satellites in the 2,2'-bipyridine proton spectrum lead to the conclusion that the reaction being followed is exchange of a coordinated water ligand with the bulk solvent.

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