The α(C—C)/β(C—H) ratio of the primary processes in the 184.9 nm photolysis of gaseous cis- and trans-2-butene

1987 ◽  
Vol 65 (7) ◽  
pp. 1631-1638 ◽  
Author(s):  
Hélène Deslauriers ◽  
Wlodzimiercz Makulski ◽  
Guy J. Collin
Keyword(s):  
Quantum Yield ◽  
Gas Phase ◽  
Molecular Property ◽  
Bond Rupture ◽  
Complete Study ◽  
Yield Values ◽  
In Trans ◽  
Cis And Trans ◽  
In Cis

A complete study of the 184.9 nm photolysis of cis- and trans-2-butene has been done in the gas phase. The main primary processes (>90%) are the α(C—C) and β(C—H) bond ruptures. Both processes have similar quantum yield values in cis-2-butene: [Formula: see text]. Conversely, the α(C—C) bond rupture is more important in trans-2-butene: [Formula: see text]. These values are compared with those measured in propene (0.57) and isobutene (0.95). No simple molecular property seems sufficient to explain such an effect.

A silencer element from the alpha-globin gene inhibits expression of beta-like genes

1988 ◽  
Vol 8 (11) ◽  
pp. 5047-5051
Author(s):  
G F Atweh ◽  
J M Liu ◽  
H E Brickner ◽  
X X Zhu
Keyword(s):  
Globin Gene ◽  
Expression System ◽  
Globin Genes ◽  
Beta Globin ◽  
Base Pair Fragment ◽  
Alpha Globin ◽  
In Trans ◽  
Cis And Trans ◽  
In Cis ◽  
Silencer Element

We have studied the cis and trans interactions of the alpha- and beta-globin genes in a transient expression system. We found that the alpha-globin gene inhibited beta-globin expression in cis but not in trans. The silencer element responsible for this inhibition was localized to a 259-base-pair fragment at the 5' end of the alpha-globin gene.

scholarly journals The Yeast Casein Kinase Yck3p Is Palmitoylated, then Sorted to the Vacuolar Membrane with AP-3-dependent Recognition of a YXXϕ Adaptin Sorting Signal

2004 ◽  
Vol 15 (3) ◽  
pp. 1397-1406 ◽  
Author(s):  
Beimeng Sun ◽  
Linyi Chen ◽  
Wei Cao ◽  
Amy F. Roth ◽  
Nicholas G. Davis
Keyword(s):  
Plasma Membrane ◽  
Mammalian Cells ◽  
Casein Kinase ◽  
Vacuolar Membrane ◽  
Sorting Signal ◽  
Casein Kinases ◽  
In Trans ◽  
Vacuolar Sorting ◽  
Cis And Trans ◽  
In Cis

Our previous work found the two yeast plasma membrane-localized casein kinases Yck1p and Yck2p to be palmitoylated on C-terminal Cys-Cys sequences by the palmitoyl transferase Akr1p. The present work examines a third casein kinase, Yck3p, which ends with the C-terminal sequence Cys-Cys-Cys-Cys-Phe-Cys-Cys-Cys. Yck3p is palmitoylated and localized to the vacuolar membrane. While the C-terminal cysteines are required for this palmitoylation, Akr1p is not. Palmitoylation requires the C-terminal Yck3p residues 463-524, whereas information for vacuolar sorting maps to the 409-462 interval. Vacuolar sorting is disrupted in cis through deletion of the 409-462 sequences and in trans through mutation of the AP-3 adaptin complex; both cis- and trans-mutations result in Yck3p missorting to the plasma membrane. This missorted Yck3p restores 37°C viability to yck1Δ yck2-ts cells. yck1Δ yck2-ts suppressor mutations isolated within the YCK3 gene identify the Yck3p vacuolar sorting signal—the tetrapeptide YDSI, a perfect fit to the YXXϕ adaptin-binding consensus. Although YXXϕ signals have a well-appreciated role in the adaptin-mediated sorting of mammalian cells, this is the first signal of this class to be identified in yeast.

Stereochemical effects in the gas-phase pinacol rearrangement. 2. Ring contraction versus methyl migration in cis- and trans-1,2-dimethylcyclohexane-1,2-diol

1988 ◽  
Vol 110 (4) ◽  
pp. 1098-1103 ◽  
Author(s):  
Giulia. De Petris ◽  
Pierluigi. Giacomello ◽  
Adriano. Pizzabiocca ◽  
Gabriele. Renzi ◽  
Maurizio. Speranza
Keyword(s):  
Gas Phase ◽  
Ring Contraction ◽  
Pinacol Rearrangement ◽  
Cis And Trans ◽  
In Cis

scholarly journals Bethe-Salpeter Study of the Optical Absorption of trans and cis Azobenzene-Functionalized Metal-Organic Frameworks using Molecular and Periodic Models

2021 ◽  
Author(s):  
Aseem Rajan Kshirsagar ◽  
Claudio Attaccalite ◽  
Xavier Blase ◽  
Jing Li ◽  
Roberta Poloni
Keyword(s):  
Optical Absorption ◽  
Gas Phase ◽  
Metal Organic Framework ◽  
Molecular Models ◽  
Gw Approximation ◽  
Periodic Models ◽  
Metal Organic ◽  
Reasonable Description ◽  
Cis And Trans ◽  
In Cis

The optical absorption spectra of the azobenzene-functionalized metal-organic framework, PCN-123, are calculated in cis and trans configurations using the Bethe-Salpeter equation (BSE) formalism and the GW approximation using periodic and non-periodic models. In the visible, near-UV and mid-UV region the optical excitations in the MOF are associated with the azobenzene functionalities and this results in spectral features similar to the case of the gas phase azobenzene and the azo-functionalized ligand. The most noticeable difference is the significantly more intense S<sub>1</sub> band for cis in the MOF as compared to the free molecules which points to a faster and more complete cis→trans isomerization in the framework, with strong implications for the design of MOFs with high photoconversion efficiencies. Consistent with these findings, all the molecular models employed to represent the MOF, including the smallest, are found to yield a reasonable description of the low energy optical spectra (between 2 and 5 eV) of the periodic framework, with the exception of the stronger S<sub>1</sub> band of cis in the MOF, a feature that we attribute to a limitation of the fragment model to correctly represent the wavefunction of the extended framework.

scholarly journals Cis and trans RET signaling control the survival and central projection growth of rapidly adapting mechanoreceptors

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Michael S Fleming ◽  
Anna Vysochan ◽  
Sόnia Paixão ◽  
Jingwen Niu ◽  
Rüdiger Klein ◽  
...  
Keyword(s):  
Cell Survival ◽  
Dorsal Root Ganglia ◽  
Dorsal Root ◽  
Developmental Process ◽  
Central Projection ◽  
Drg Neurons ◽  
In Trans ◽  
Cis And Trans ◽  
In Cis

RET can be activated in cis or trans by its co-receptors and ligands in vitro, but the physiological roles of trans signaling are unclear. Rapidly adapting (RA) mechanoreceptors in dorsal root ganglia (DRGs) express Ret and the co-receptor Gfrα2 and depend on Ret for survival and central projection growth. Here, we show that Ret and Gfrα2 null mice display comparable early central projection deficits, but Gfrα2 null RA mechanoreceptors recover later. Loss of Gfrα1, the co-receptor implicated in activating RET in trans, causes no significant central projection or cell survival deficit, but Gfrα1;Gfrα2 double nulls phenocopy Ret nulls. Finally, we demonstrate that GFRα1 produced by neighboring DRG neurons activates RET in RA mechanoreceptors. Taken together, our results suggest that trans and cis RET signaling could function in the same developmental process and that the availability of both forms of activation likely enhances but not diversifies outcomes of RET signaling.

scholarly journals A silencer element from the alpha-globin gene inhibits expression of beta-like genes.

1988 ◽  
Vol 8 (11) ◽  
pp. 5047-5051 ◽  
Author(s):  
G F Atweh ◽  
J M Liu ◽  
H E Brickner ◽  
X X Zhu
Keyword(s):  
Globin Gene ◽  
Expression System ◽  
Globin Genes ◽  
Beta Globin ◽  
Base Pair Fragment ◽  
Alpha Globin ◽  
In Trans ◽  
Cis And Trans ◽  
In Cis ◽  
Silencer Element

We have studied the cis and trans interactions of the alpha- and beta-globin genes in a transient expression system. We found that the alpha-globin gene inhibited beta-globin expression in cis but not in trans. The silencer element responsible for this inhibition was localized to a 259-base-pair fragment at the 5' end of the alpha-globin gene.

scholarly journals Polarized secretion of lysosomal enzymes: co-distribution of cation-independent mannose-6-phosphate receptors and lysosomal enzymes along the osteoclast exocytic pathway.

1988 ◽  
Vol 106 (6) ◽  
pp. 1863-1872 ◽  
Author(s):  
R Baron ◽  
L Neff ◽  
W Brown ◽  
P J Courtoy ◽  
D Louvard ◽  
...  
Keyword(s):  
Lysosomal Enzymes ◽  
Polarized Secretion ◽  
Transport Vesicles ◽  
Immunocytochemical Techniques ◽  
In Trans ◽  
Ruffled Border ◽  
Mannose 6 Phosphate ◽  
Exocytic Pathway ◽  
Cis And Trans ◽  
In Cis

The osteoclast is a polarized cell which secretes large amounts of newly synthesized lysosomal enzymes into an apical extracellular lacuna where bone resorption takes place. Using immunocytochemical techniques, we have localized the cation-independent mannose-6-phosphate (Man6P) receptor and lysosomal enzymes in this cell type in order to determine the expression and distribution of this receptor and its ligands. The results demonstrate that the osteoclast expresses large amounts of immunoreactive cation-independent Man6P receptors, despite the fact that most of the lysosomal enzymes it synthesizes are secreted. The lysosomal enzymes and the receptors are co-distributed along the exocytic pathway, i.e., the endoplasmic reticulum, including the perinuclear envelope, the Golgi stacks as well as numerous small transport vesicles that appear to fuse with the ruffled border membrane. Within the Golgi complex, the receptors and lysosomal enzymes were found distributed in two predominant patterns; (a) in all the cisternae, from cis to trans, or (b) predominantly in cis- and trans-Golgi cisternae, with the middle Golgi cisternae being unstained or depleted in antigen. This pattern suggests that enzymes and receptors traverse the Golgi from cis to trans and preferentially accumulate in cis- and in trans-cisternae. This study therefore suggests that, in the osteoclast, Man6P receptors are involved in the vectorial transport and targeting of newly synthesized lysosomal enzymes, presumably via a constitutive pathway, to the apical membrane where they are secreted into the bone-resorbing compartment. This mechanism could insure polarized secretion of lysosomal enzymes into the bone-resorbing lacuna.

scholarly journals Bethe-Salpeter Study of the Optical Absorption of trans and cis Azobenzene-Functionalized Metal-Organic Frameworks using Molecular and Periodic Models

2021 ◽  
Author(s):  
Aseem Rajan Kshirsagar ◽  
Claudio Attaccalite ◽  
Xavier Blase ◽  
Jing Li ◽  
Roberta Poloni
Keyword(s):  
Optical Absorption ◽  
Gas Phase ◽  
Metal Organic Framework ◽  
Molecular Models ◽  
Gw Approximation ◽  
Periodic Models ◽  
Metal Organic ◽  
Reasonable Description ◽  
Cis And Trans ◽  
In Cis

The optical absorption spectra of the azobenzene-functionalized metal-organic framework, PCN-123, are calculated in cis and trans configurations using the Bethe-Salpeter equation (BSE) formalism and the GW approximation using periodic and non-periodic models. In the visible, near-UV and mid-UV region the optical excitations in the MOF are associated with the azobenzene functionalities and this results in spectral features similar to the case of the gas phase azobenzene and the azo-functionalized ligand. The most noticeable difference is the significantly more intense S<sub>1</sub> band for cis in the MOF as compared to the free molecules which points to a faster and more complete cis→trans isomerization in the framework, with strong implications for the design of MOFs with high photoconversion efficiencies. Consistent with these findings, all the molecular models employed to represent the MOF, including the smallest, are found to yield a reasonable description of the low energy optical spectra (between 2 and 5 eV) of the periodic framework, with the exception of the stronger S<sub>1</sub> band of cis in the MOF, a feature that we attribute to a limitation of the fragment model to correctly represent the wavefunction of the extended framework.

scholarly journals Locus-associated R-loop repression in cis

2021 ◽  
Author(s):  
Negin Khosraviani ◽  
Karan J. Abraham ◽  
Janet N. Y. Chan ◽  
Karim Mekhail
Keyword(s):  
Genetic Locus ◽  
Proximal Site ◽  
In Trans ◽  
Study Designs ◽  
Cis And Trans ◽  
In Cis

R-loops exert varied beneficial or detrimental effects. To assess the function of an R-loop at a specific genetic locus, we had developed an inducible RNaseH1-EGFP-dCas9 (RED) protein chimaera as part of a locus-associated R-loop-modulating system (LasR). LasR is compatible with R-loop modulation in trans, which targets RED to one locus to repress R-loops at another spatially proximal site. Here we use the LasR system for R-loop modulation in cis, which consists of targeting RED directly to an R-loop. The combination of LasR in cis and trans will be essential to ascribe functions to specific R-loops within varied molecular contexts and study designs.

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