Studies on γ-irradiated aqueous solutions of amino acids with functional groups by the spin-trap high-performance liquid chromatography – electron spin resonance method

Aqueous solutions of L-glutamine, L-asparagine, sodium L-glutamate, sodium L-aspartate, L-serine, and L-threonine were γ-irradiated in the presence of a spin trap, 2-methyl-2-nitrosopropane. Stable spin adducts produced in the irradiated solutions were analyzed by the spin-trap hplc–esr method. The spin adducts of the following short-lived radicals were found and identified: H2NCOCH2CH2ĊHCOO− (1), [Formula: see text] and [Formula: see text] for L-glutamine; [Formula: see text] (2) for L-asparagine; −OOCCH2CH2ĊHCOO− (3) and [Formula: see text] for sodium L-glutamate; −OOCCH2ĊHCOO− (4) and [Formula: see text] (5) for sodium L-aspartate; [Formula: see text] and HOCH2ĊHCOO− (6) for L-serine; CH3CH(OH)ĊHCOO− (7) and [Formula: see text] for L-threonine. The free radicals, 1, 3, 4, 6, and 7, were due to the reaction of the hydrated electron [Formula: see text] with these amino acids and the other adducts were due to the attack of hydroxyl radical (•OH). The two diastereomeric pairs of spin adducts of the short-lived radicals, 2 and 5, could be individually separated by the technique utilized.