Glucans from Candida albicans (serotype B) and from Candida parapsilosis

1967 ◽  
Vol 45 (19) ◽  
pp. 2264-2267 ◽  
Author(s):  
R. J. Yu ◽  
C. T. Bishop ◽  
F. Blank ◽  
H. F. Hasenclever
Keyword(s):  
Candida Albicans ◽  
Candida Parapsilosis ◽  
Serotype B

Structural studies of mannans from Candida albicans (serotypes A and B), Candida parapsilosis, Candida stellatoidea, and Candida tropicalis

1967 ◽  
Vol 45 (19) ◽  
pp. 2205-2211 ◽  
Author(s):  
R. J. Yu ◽  
C. T. Bishop ◽  
F. P. Cooper ◽  
H. F. Hasenclever ◽  
F. Blank
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Species ◽  
Candida Parapsilosis ◽  
Cross Reactivity ◽  
Structural Studies ◽  
Serotype A ◽  
Serotype B ◽  
Hydrolysis Of

Mannans have been isolated from cells of the following Candida species: C. albicans (serotype A), C. albicans (serotype B), C. parapsilosis, C. stellatoidea, and C. tropicalis. Methylation and hydrolysis of each mannan yielded the following methyl ethers of d-mannose (with only small variations in the relative amounts): 2,3,4,6-tetra-O-methyl-d-mannose, 3,4,6-tri-O-methyl-d-mannose (major), 2,3,4-tri-O-methyl-d-mannose (minor), 2,4,6-tri-O-methyl-d-mannose (minor), 3,4-di-O-methyl-d-mannose, and 3,5-di-O-methyl-d-mannose. The mannans therefore contained a predominance of 1 → 2 linkages in the linear portions, with smaller amounts of 1 → 6 and 1 → 3 linkages. Branching occurred through C-2 and C-6 of d-mannopyranose and d-mannofuranose units, and branches were terminated by d-mannopyranose units. The specific rotations of the mannans indicated that most of the glycosidic linkages were in the α configuration. The structural studies support the observation that the mannans are very similar serologically and show cross-reactivity in antisera to any of the parent organisms.

scholarly journals Infecções oportunistas por leveduras e perfil enzimático dos agentes etiológicos

2009 ◽  
Vol 42 (2) ◽  
pp. 188-191 ◽  
Author(s):  
Danielle Patrícia Cerqueira Macêdo ◽  
Aline Mary de Almeida Farias ◽  
Reginaldo Gonçalves de Lima Neto ◽  
Vanessa Karina Alves da Silva ◽  
André Ferraz Goiana Leal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei

Infecções por leveduras são freqüentes em imunocomprometidos, contudo espécies emergentes têm alterado o perfil epidemiológico. A habilidade de secretar proteases tem sido associada à patogenicidade do gênero Candida. Esta pesquisa teve como objetivos diagnosticar leveduroses em pacientes imunocomprometidos e avaliar a virulência dos agentes etiológicos baseado em teste de secreção de protease utilizando soro de albumina bovina como substrato. Do total de 104 pacientes estudados, 19 apresentaram episódios de leveduroses. O trato respiratório (63,2%), seguido pelo trato urinário (10,5%) foram os locais mais comuns de infecção. Candida albicans, Candida parapsilosis, Candida tropicalis e espécies emergentes como Candida krusei e Candida guilliermondii foram isoladas. Cinco isolados de Candida parapsilosis e um de Candida albicans e Candida guilliermondii exibiram alta atividade enzimática. Concluímos que a caracterização enzimática de isolados de Candida pode ser um útil marcador prognóstico, especialmente em imunocomprometidos, uma vez que leveduroses nestes pacientes são geralmente graves.

scholarly journals Differential Activities of Newer Antifungal Agents against Candida albicans and Candida parapsilosis Biofilms

2007 ◽  
Vol 52 (1) ◽  
pp. 357-360 ◽  
Author(s):  
Αspasia Katragkou ◽  
Athanasios Chatzimoschou ◽  
Maria Simitsopoulou ◽  
Maria Dalakiouridou ◽  
Eudoxia Diza-Mataftsi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Agents ◽  
Candida Parapsilosis ◽  
Planktonic Cells

ABSTRACT The activities of voriconazole, posaconazole, caspofungin, and anidulafungin against Candida albicans and Candida parapsilosis biofilms were evaluated. In contrast to planktonic cells, the MICs for voriconazole and posaconazole for the biofilms of the two species were high (≥256 and >64 mg/liter, respectively) but relatively low for the echinocandins caspofungin and anidulafungin (≤1 and ≤2 mg/liter, respectively).

scholarly journals Conserved and Divergent Roles of Bcr1 and CFEM Proteins in Candida parapsilosis and Candida albicans

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28151 ◽  
Author(s):  
Chen Ding ◽  
Genevieve M. Vidanes ◽  
Sarah L. Maguire ◽  
Alessandro Guida ◽  
John M. Synnott ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Parapsilosis

scholarly journals INCIDENCES OF CANDIDA ALBICANS AND NON-ALBICANS AMONG CATHETER-ASSOCIATED URINARY TRACT INFECTION PATIENTS OF AKOLA CITY

2017 ◽  
Vol 10 (11) ◽  
pp. 115
Author(s):  
Khandare Ln ◽  
Barate Dl
Keyword(s):  
Urinary Tract Infection ◽  
Candida Albicans ◽  
Urinary Tract ◽  
Tract Infection ◽  
Candida Parapsilosis ◽  
Opportunistic Infections ◽  
Urine Samples ◽  
Age Group ◽  
Candida Spp ◽  
Catheter Urine

Objective: Candida spp. is the third leading cause of catheter-related infections. Candida species is a part of human microflora and it becomes pathogenic when certain conditions are present and cause an opportunistic infections. The present study was undertaken to determine incidences of Candida albicans and non-albicans among catheterized urinary tract infection (UTI) patients of Akola city.Methods: A total 60 catheter urine samples were collected from patient of all the age group and both sex who had indwelling urinary catheter. The collected catheterized urine samples of patients from various hospitals of Akola city were used for isolation using HiCrome Candida differential agar.Results: It was found that highest frequency of isolation of Candida spp. was from age group 61-70 years. The predominance of male candidate was more than female having Candida spp. in catheter-associated UTI (C-UTI). Among the Candida spp. C. albicans (64.81%) was predominant over non-albicans spp. while in non-albicans Candida krusei and Candida glabrata were predominant showing 11.11% incidences. It was followed by Candida tropicalis (9.2%) and Candida parapsilosis (3.7%).Conclusion: The incidences of C. albicans and non-albicans were high among catheter-associated UTI patients.

Extracellular Aspartic Proteinases from Candida albicans, Candida tropicalis, and Candida parapsilosis Yeasts Differ Substantially in Their Specificities

Biochemistry ◽  
1994 ◽  
Vol 33 (32) ◽  
pp. 9791-9799 ◽  
Author(s):  
Martin Fusek ◽  
Elizabeth A. Smith ◽  
Michael Monod ◽  
Ben M. Dunn ◽  
Stephen I. Foundling
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Parapsilosis ◽  
Aspartic Proteinases

scholarly journals Aspectos micológicos e suscetibilidade in vitro de leveduras do gênero Candida em pacientes HIV-positivos provenientes do Estado de Mato Grosso

2010 ◽  
Vol 43 (6) ◽  
pp. 673-677 ◽  
Author(s):  
Olivia Cometti Favalessa ◽  
Marilena dos Anjos Martins ◽  
Rosane Christine Hahn
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Mato Grosso ◽  
Candida Sp

INTRODUÇÃO: A candidíase é uma das infecções fúngicas mais frequentes entre os pacientes infectados pelo vírus da imunodeficiência humana. O presente estudo objetivou a caracterização das leveduras do gênero Candida de distintas amostras clínicas, provenientes de pacientes HIV - positivos, assim como a determinação do perfil de suscetibilidade in vitro a cinco drogas antifúngicas. MÉTODOS: A caracterização dos isolados de Candida sp foi realizada através da metodologia clássica, testes bioquímicos (zimograma e auxanograma) e morfológicos (prova do tubo germinativo e microcultivo em lâmina). Também, foram realizadas a técnica genotípica (PCR) e identificação pelo método comercial API 20C AUX (BioMeriéux). Para a determinação do perfil de suscetibilidade in vitro, foram utilizadas cinco drogas antifúngicas (cetoconazol, fluconazol, itraconazol, voriconazol e anfotericina B), através do método comercialmente disponível - Etest. RESULTADOS: Foram identificados 105 isolados de leveduras do gênero Candida provenientes de 102 pacientes infectados pelo vírus HIV. Destes, foram caracterizadas 82 (78,1%) Candida albicans, 8 (7,6%) Candida parapsilosis, 8 (7,6%) Candida tropicalis, 4 (3,8%) Candida krusei, 2 (1,9%) Candida glabrata e 1 (1%) Candida guilliermondii. CONCLUSÕES: Considerando o perfil geral de sensibilidade, 60% dos isolados foram suscetíveis a todos os antifúngicos testados, porém as espécies C. tropicalis e C. krusei demonstraram uma tendência a valores mais elevados de CIMs para os azóis do que os encontrados paraC. albicans, sugerindo resistência.

scholarly journals Biological Activities of Naturally Occurring Antibodies Reactive with Candida albicans Mannan

2004 ◽  
Vol 72 (1) ◽  
pp. 209-218 ◽  
Author(s):  
Thomas R. Kozel ◽  
Randall S. MacGill ◽  
Ann Percival ◽  
Qing Zhou
Keyword(s):  
Candida Albicans ◽  
B Cells ◽  
Complement System ◽  
Biological Activities ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serotype A ◽  
Naturally Occurring ◽  
Serotype B ◽  
Opsonophagocytic Killing ◽  
The Complement System

ABSTRACT Sera from normal adult humans may contain high levels of antibody reactive with Candida albicans mannan. This study examined selected biological activities of such antibodies, focusing on sera that were collected from 34 donors and analyzed individually. The results showed that antimannan titers were normally distributed. Reactivity as determined by enzyme-linked immunosorbent assay with serotype A mannan generally paralleled reactivity with serotype B. Analysis of the kinetics for activation of the complement system and deposition of complement component 3 (C3) onto serotype A and serotype B cells showed a decrease in the lag time that occurred before the onset of rapid accumulation of C3 that correlated with increasing antimannan titers. In contrast, there was a decrease in the overall rate of accumulation of C3 on serotype A cells that was strongly correlated with increasing antibody titers; serotype B cells showed no such decrease. An evaluation of the contribution of mannan antibody to opsonophagocytic killing showed that mannan antibody in individual sera and antimannan immunoglobulin G (IgG) affinity purified from human plasma contributed to killing by neutrophils in a dose-dependent fashion in the absence of a functional complement system. However, affinity-purified antibody in very high concentrations was inhibitory to both complement-dependent and complement-independent opsonophagocytosis, and this finding suggests a prozone-like effect. In contrast, if the complement system was functional, antimannan IgG was not needed for opsonophagocytic killing. These results suggest that naturally occurring mannan antibodies and the complement system are functionally redundant for opsonophagocytic killing by neutrophils.

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