PREPARATION, THIN LAYER CHROMATOGRAPHY, AND ULTRAVIOLET SPECTRA OF SOME O-BENZYL DERIVATIVES OF D-GLUCOSE

1963 ◽  
Vol 41 (7) ◽  
pp. 1801-1806 ◽  
Author(s):  
M. E. Tate ◽  
C. T. Bishop
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Absorption Spectroscopy ◽  
Preparative Method ◽  
New Method ◽  
Hydroxyl Groups ◽  
Good Criterion ◽  
Ultraviolet Absorption Spectroscopy ◽  
Layer Chromatography ◽  
Derivatives Of

A new method for benzylation of carbohydrates has been developed which facilitated complete substitution of hydroxyl groups in one of the compounds prepared. Thin layer chromatography provides a good criterion of purity and is also a preparative method for separation of identifiable amounts of O-benzyl derivatives of carbohydrates. The number of benzyl groups in a compound can be determined by ultraviolet absorption spectroscopy. The following compounds were prepared, some of which have not been reported previously: benzyl-2,3,4,6-tetra-O-benzyl-α-D-glucoside; benzyl 2,3,4,6-tetra-O-benzyl-β-D-glucoside; methyl 2,3,4,6-tetra-O-benzyl-α-D-glucoside; 2,3,4,6-tetra-O-benzyl-α-D-glucose; l-O-(p-nitro-benzoyl)-2,3,4,6-tetra-O-benzyl-α-D-glucose; octa-O-benzyl sucrose; 3,5-di-O-benzyl-1,2-O-cyclohexylidene-α-D-xylose.

Oscillopolarographic Analysis of Morestan® Residues in Valencia Orange Rind Following Thin Layer Chromatography

1966 ◽  
Vol 49 (4) ◽  
pp. 774-778
Author(s):  
Fred E Hearth ◽  
Daniel E Ott ◽  
Francis A Gunther
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Colorimetric Method ◽  
New Method ◽  
Valencia Orange ◽  
Layer Chromatography

Abstract A new method combining oscillopolarography and thin layer chromatography is described for the microdetermination of residual Morestan in Valencia orange rind; 0.5 ppm is readily detectable, but accurate quantitation is difficult below 1.0 ppm. Data obtained by this method are comparable to those from a colorimetric method.

A new method to detect homocysteine in dried blood spots using thin layer chromatography

Screening ◽  
1995 ◽  
Vol 4 (1) ◽  
pp. 17-26 ◽  
Author(s):  
H. Ohtake ◽  
Y. Hase ◽  
K. Sakemoto ◽  
T. Oura ◽  
Y. Wada ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Dried Blood Spots ◽  
New Method ◽  
Blood Spots ◽  
Layer Chromatography

scholarly journals The reaction of iodobenzene-p-sulphonyl chloride (pipsyl chloride) with certain amino acids and peptides, and with insulin

1967 ◽  
Vol 102 (3) ◽  
pp. 815-824 ◽  
Author(s):  
J. C. Fletcher
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Cysteic Acid ◽  
Buffer System ◽  
The Common ◽  
Layer Chromatography ◽  
Derivatives Of

1. A system of separation using buffered Celite columns is described that enables the pipsyl derivatives of most of the common amino acids to be separated. 2. The reaction of pipsyl chloride with several amino acids not included in previous studies has been investigated. In particular, knowledge of the acid-soluble pipsyl derivatives of arginine, histidine, lysine, tyrosine and cysteic acid has been extended. 3. Reproducible factors have been obtained that enable corrections to be applied for the breakdown of pipsylamino acids on acid hydrolysis. 4. The reaction of pipsyl chloride with peptides has been studied under various conditions. 5. The extent of the reaction between pipsyl chloride and insulin depends on the nature of the solvent-buffer system, and under the best conditions so far found is about 75% complete. 6. In an Appendix, the separation of pipsylamino acids by thin-layer chromatography is described.

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