CONSTRUCTION AND USE OF A SEMIMICRO LIGHT-SCATTERING APPARATUS

1953 ◽  
Vol 31 (11) ◽  
pp. 1078-1092 ◽  
Author(s):  
D. A. I. Goring
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Rayleigh Scattering ◽  
Colloidal Silica ◽  
Incident Beam ◽  
Calibration Factor ◽  
Scattering System

A light-scattering apparatus requiring only 4 ml. of liquid was designed and built. The device has permitted the measurement of intensity for angles between [Formula: see text] and 135° to the incident beam. In this range the unpolarized, horizontal, and vertical components of the light emitted from a solution of fluorescein were constant to within ± 0.2%, 0.4%, and 0.6% respectively. The apparatus was calibrated with a suspension of Ludox colloidal silica which absorbed no light for λ = 3800 Å to 6000 Å. The suspension was found to possess all the properties of a Rayleigh scattering system except randomness. This condition was achieved by extrapolating the light-scattering and spectrophotometric functions to zero concentration when computing the calibration factor. The molecular weight of a sample of sodium carrageenate, determined by the method of Zimm, was 1,700,000. The distribution of intensity at. zero concentration fell between that for stiff rods and polydisperse coils.

A light-scattering study of the effect of calcium chloride on the molecular weight of busycon hemocyanin

1978 ◽  
Vol 8 (2) ◽  
pp. 133-138 ◽  
Author(s):  
M.Constance Loeffler ◽  
Shian-shi Su ◽  
Norman C. Li ◽  
Edward F. Casassa
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Calcium Chloride ◽  
Scattering Study

A STUDY OF A WATER-SOLUBLE COMPLEX OF PRODIGIOSIN PRODUCED BY A STRAIN OF SERRATIA MARCESCENS

1962 ◽  
Vol 40 (1) ◽  
pp. 1019-1024 ◽  
Author(s):  
Seiichi Yoshida
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Serratia Marcescens ◽  
Average Molecular Weight ◽  
Electrophoretic Analysis ◽  
Water Soluble ◽  
Soluble Complex ◽  
Ph Values ◽  
Scattering Measurements ◽  
Water Soluble Complex

A water-soluble pigment excreted from Serratia marcescens has been purified by precipitation with ammonium sulphate, dialysis, and ultracentrifugation at different pH values. The purified pigment showed a single band in the ultracentrifuge and by electrophoretic analysis at several pH values. An average molecular weight of 5 × 106 was calculated from light-scattering measurements. This pigment is composed of carbohydrate and protein combined with prodigiosin, and several properties of the complex are described.

A Progress in Development and Application of Size Exclusion Chromatograph/Multi-Angle Laser Light Scattering

2014 ◽  
Vol 936 ◽  
pp. 1597-1602
Author(s):  
Xue Yang ◽  
Ze Quan Liu ◽  
Qiang Zhang
Keyword(s):  
Molecular Weight ◽  
Light Scattering ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Laser Light ◽  
Laser Light Scattering ◽  
Size Exclusion

This paper reveals the development and significance of size exclusion chromatograph/multi-angle laser light scattering(SEC-MALLS) for determination of the molecular weight and characterization of the molecular weight distribution. This technque has been applied in many aspects, such as biology, polymer and carboxylate surfactant. It has made a big contribution to study the property of macromolecular substances.

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